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Protein

Chromobox protein homolog 5

Gene

Cbx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins (By similarity).By similarity

GO - Molecular functioni

  • chromatin binding Source: MGI
  • histone deacetylase binding Source: BHF-UCL
  • identical protein binding Source: MGI
  • methylated histone binding Source: MGI
  • protein binding, bridging Source: BHF-UCL
  • protein-containing complex binding Source: UniProtKB
  • protein homodimerization activity Source: MGI
  • repressing transcription factor binding Source: BHF-UCL
  • ribonucleoprotein complex binding Source: UniProtKB

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 5
Alternative name(s):
Heterochromatin protein 1 homolog alpha
Short name:
HP1 alpha
Gene namesi
Name:Cbx5
Synonyms:Hp1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:109372 Cbx5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802091 – 191Chromobox protein homolog 5Add BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineBy similarity1
Modified residuei12PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei14PhosphoserineCombined sources1
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei40N6-acetyllysineBy similarity1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei92PhosphoserineCombined sources1
Modified residuei93PhosphoserineCombined sources1
Modified residuei95PhosphoserineBy similarity1
Modified residuei97PhosphoserineBy similarity1
Cross-linki102Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki106Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki184Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis (By similarity).By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61686
MaxQBiQ61686
PaxDbiQ61686
PeptideAtlasiQ61686
PRIDEiQ61686

PTM databases

iPTMnetiQ61686
PhosphoSitePlusiQ61686

Expressioni

Gene expression databases

BgeeiENSMUSG00000009575
CleanExiMM_CBX5
ExpressionAtlasiQ61686 baseline and differential
GenevisibleiQ61686 MM

Interactioni

Subunit structurei

Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100 and STAM2 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with BAHD1, SETDB1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP. Interacts with CHAMP1 (By similarity). Interacts directly with TRIM28 via the chromoshadow domain (PubMed:8978696). Interacts with KMT5B and KMT5C (PubMed:15145825). Interacts with HP1BP3 and TRIM24 (PubMed:8978696). May form homodimers (PubMed:8978696). Interacts with ASXL1 (By similarity). Interacts with PRR14 (via N-terminus) (By similarity). Interacts with RRP1B (By similarity). Interacts with HNRNPU (via C-terminus); this interaction is, at least in part, RNA-dependent (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • histone deacetylase binding Source: BHF-UCL
  • identical protein binding Source: MGI
  • methylated histone binding Source: MGI
  • protein binding, bridging Source: BHF-UCL
  • protein homodimerization activity Source: MGI
  • repressing transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi198538, 25 interactors
DIPiDIP-28137N
IntActiQ61686, 11 interactors
MINTiQ61686
STRINGi10090.ENSMUSP00000104441

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 14Combined sources3
Beta strandi22 – 31Combined sources10
Beta strandi34 – 41Combined sources8
Helixi46 – 48Combined sources3
Beta strandi51 – 53Combined sources3
Turni54 – 56Combined sources3
Helixi60 – 73Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RVLNMR-A1-80[»]
2RVMNMR-A1-80[»]
2RVNNMR-A1-80[»]
ProteinModelPortaliQ61686
SMRiQ61686
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 78Chromo 1PROSITE-ProRule annotationAdd BLAST59
Domaini121 – 179Chromo 2; shadow subtypePROSITE-ProRule annotationAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 14Poly-Ser4

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410KDV4 Eukaryota
ENOG4111I3N LUCA
GeneTreeiENSGT00510000046310
HOGENOMiHOG000220852
HOVERGENiHBG000400
InParanoidiQ61686
KOiK11587
OMAiYSEKHNT
OrthoDBiEOG091G0RBS
PhylomeDBiQ61686
TreeFamiTF350503

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR017984 Chromo_dom_subgr
IPR023780 Chromo_domain
IPR008251 Chromo_shadow_dom
IPR023779 Chromodomain_CS
PfamiView protein in Pfam
PF00385 Chromo, 1 hit
PF01393 Chromo_shadow, 1 hit
PRINTSiPR00504 CHROMODOMAIN
SMARTiView protein in SMART
SM00298 CHROMO, 2 hits
SM00300 ChSh, 1 hit
SUPFAMiSSF54160 SSF54160, 2 hits
PROSITEiView protein in PROSITE
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q61686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKTKRTAD SSSSEDEEEY VVEKVLDRRM VKGQVEYLLK WKGFSEEHNT
60 70 80 90 100
WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSEGNKR KSSFSNSADD
110 120 130 140 150
IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL
160 170 180 190
VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKESAK S
Length:191
Mass (Da):22,186
Last modified:November 1, 1997 - v1
Checksum:i8AB87E2F00DB8C40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68K → R in BAB31173 (PubMed:16141072).Curated1
Sequence conflicti165I → M in BAB31596 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99641 mRNA Translation: CAA67960.1
AF216290 mRNA Translation: AAF80993.1
AK008792 mRNA Translation: BAB25897.1
AK018349 mRNA Translation: BAB31173.1
AK030366 mRNA Translation: BAC26923.1
AK030442 mRNA Translation: BAC26966.1
AK032975 mRNA Translation: BAC28107.1
AK019198 mRNA Translation: BAB31596.1
BC004707 mRNA Translation: AAH04707.1
CCDSiCCDS37231.1
RefSeqiNP_001070257.1, NM_001076789.1
NP_001103686.1, NM_001110216.1
NP_031652.1, NM_007626.3
XP_006520435.1, XM_006520372.3
XP_006520437.1, XM_006520374.3
XP_006520438.1, XM_006520375.3
XP_006520439.1, XM_006520376.3
UniGeneiMm.262059

Genome annotation databases

EnsembliENSMUST00000108813; ENSMUSP00000104441; ENSMUSG00000009575
ENSMUST00000118152; ENSMUSP00000113157; ENSMUSG00000009575
ENSMUST00000122182; ENSMUSP00000113158; ENSMUSG00000009575
GeneIDi12419
KEGGimmu:12419
UCSCiuc007xxl.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCBX5_MOUSE
AccessioniPrimary (citable) accession number: Q61686
Secondary accession number(s): Q9CS35, Q9CXD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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