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Protein

Equilibrative nucleoside transporter 2

Gene

Slc29a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates equilibrative transport of purine and pyrimidine nucleosides, and the purine base hypoxanthine.

Kineticsi

  1. KM=0.75 mM for adenosine

    GO - Molecular functioni

    • nucleoside transmembrane transporter activity Source: MGI

    GO - Biological processi

    • nucleoside transmembrane transport Source: GOC
    • nucleoside transport Source: MGI
    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

    Protein family/group databases

    TCDBi2.A.57.1.2. the equilibrative nucleoside transporter (ent) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Equilibrative nucleoside transporter 2
    Alternative name(s):
    36 kDa hydrophobic nucleolar protein
    36 kDa nucleolar protein HNP36
    Delayed-early response protein 12
    Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter
    Short name:
    Equilibrative NBMPR-insensitive nucleoside transporter
    Nucleoside transporter, ei-type
    Solute carrier family 29 member 2
    Gene namesi
    Name:Slc29a2
    Synonyms:Der12, Ent2, Hnp36
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 19

    Organism-specific databases

    MGIiMGI:1345278. Slc29a2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei98 – 11821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei161 – 18121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei192 – 21221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei323 – 34321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei360 – 38021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei396 – 41621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei432 – 45221HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456Equilibrative nucleoside transporter 2PRO_0000209341Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Modified residuei252 – 2521PhosphoserineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ61672.
    PRIDEiQ61672.

    PTM databases

    PhosphoSiteiQ61672.

    Expressioni

    Inductioni

    By platelet derived growth factor (PDGF) and fibroblast growth factor (FGF).

    Gene expression databases

    BgeeiQ61672.
    GenevisibleiQ61672. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025826.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61672.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249415.
    GeneTreeiENSGT00390000002232.
    HOGENOMiHOG000007684.
    HOVERGENiHBG074626.
    InParanoidiQ61672.
    KOiK15014.
    OMAiFNIMDCL.
    OrthoDBiEOG7PP56W.
    PhylomeDBiQ61672.
    TreeFamiTF313950.

    Family and domain databases

    InterProiIPR030197. ENT2.
    IPR002259. Eqnu_transpt.
    IPR020846. MFS_dom.
    [Graphical view]
    PANTHERiPTHR10332. PTHR10332. 1 hit.
    PTHR10332:SF8. PTHR10332:SF8. 1 hit.
    PfamiPF01733. Nucleoside_tran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016379. ENT. 1 hit.
    PRINTSiPR01130. DERENTRNSPRT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00939. 2a57. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q61672-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MARGNAPRDS YHLVGISFFI LGLGTLLPWN FFITAIPYFQ GRLAGTNSSA
    60 70 80 90 100
    ETMGTNHTSP TDTFNFNNWV TLLSQLPLLL FTLLNSFLYQ CIPESVRILG
    110 120 130 140 150
    SLLAILLLFA LTAALVKVDL SPGLFFSVTM ASVWFINSFC AVLQGSLFGQ
    160 170 180 190 200
    LGTMPSTYST LFLSGQGLAG IFAALAMLMS LASGVDAQTS ALGYFITPCV
    210 220 230 240 250
    GILLSIVCYL SLPHLKFARY YLTEKLSQAP TQELETKAEL LQADEKNGVP
    260 270 280 290 300
    ISPQQASPTL DLDPEKEPEP EEPQKPGKPS VFVVFRKIWL TALCLVLVFT
    310 320 330 340 350
    VTLSVFPAIT AMVTTSSNSP GKWGLFFNPI CCFLLFNVMD WLGRSLTSYF
    360 370 380 390 400
    LWPDEDSQQL LPLLVCLRFL FVPLFMLCHV PQHARLPIIF RQDAYFITFM
    410 420 430 440 450
    LLFAVSNGYL VSLTMCLAPR QVLPHEREVA GALMTFFLAL GLSCGASLSF

    LFKALL
    Length:456
    Mass (Da):50,255
    Last modified:March 8, 2011 - v2
    Checksum:iA9FE7CA037A4468F
    GO

    Sequence cautioni

    The sequence CAA60381.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF183397 mRNA. Translation: AAF78477.1.
    CH466612 Genomic DNA. Translation: EDL33088.1.
    BC048958 mRNA. Translation: AAH48958.1.
    X86682 mRNA. Translation: CAA60381.1. Different initiation.
    CCDSiCCDS50354.1.
    PIRiJC4195.
    RefSeqiNP_031880.2. NM_007854.3.
    UniGeneiMm.4930.

    Genome annotation databases

    EnsembliENSMUST00000025826; ENSMUSP00000025826; ENSMUSG00000024891.
    GeneIDi13340.
    KEGGimmu:13340.
    UCSCiuc008gbv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF183397 mRNA. Translation: AAF78477.1.
    CH466612 Genomic DNA. Translation: EDL33088.1.
    BC048958 mRNA. Translation: AAH48958.1.
    X86682 mRNA. Translation: CAA60381.1. Different initiation.
    CCDSiCCDS50354.1.
    PIRiJC4195.
    RefSeqiNP_031880.2. NM_007854.3.
    UniGeneiMm.4930.

    3D structure databases

    ProteinModelPortaliQ61672.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025826.

    Chemistry

    ChEMBLiCHEMBL1287612.

    Protein family/group databases

    TCDBi2.A.57.1.2. the equilibrative nucleoside transporter (ent) family.

    PTM databases

    PhosphoSiteiQ61672.

    Proteomic databases

    MaxQBiQ61672.
    PRIDEiQ61672.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000025826; ENSMUSP00000025826; ENSMUSG00000024891.
    GeneIDi13340.
    KEGGimmu:13340.
    UCSCiuc008gbv.2. mouse.

    Organism-specific databases

    CTDi3177.
    MGIiMGI:1345278. Slc29a2.

    Phylogenomic databases

    eggNOGiNOG249415.
    GeneTreeiENSGT00390000002232.
    HOGENOMiHOG000007684.
    HOVERGENiHBG074626.
    InParanoidiQ61672.
    KOiK15014.
    OMAiFNIMDCL.
    OrthoDBiEOG7PP56W.
    PhylomeDBiQ61672.
    TreeFamiTF313950.

    Enzyme and pathway databases

    ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

    Miscellaneous databases

    NextBioi283654.
    PROiQ61672.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ61672.
    GenevisibleiQ61672. MM.

    Family and domain databases

    InterProiIPR030197. ENT2.
    IPR002259. Eqnu_transpt.
    IPR020846. MFS_dom.
    [Graphical view]
    PANTHERiPTHR10332. PTHR10332. 1 hit.
    PTHR10332:SF8. PTHR10332:SF8. 1 hit.
    PfamiPF01733. Nucleoside_tran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016379. ENT. 1 hit.
    PRINTSiPR01130. DERENTRNSPRT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00939. 2a57. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain."
      Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.
      Biochem. J. 352:363-372(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD-1.
      Tissue: Brain.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    4. "A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein."
      Williams J.B., Lanahan A.A.
      Biochem. Biophys. Res. Commun. 213:325-333(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 64-456.
      Strain: BALB/c.
      Tissue: Fibroblast.

    Entry informationi

    Entry nameiS29A2_MOUSE
    AccessioniPrimary (citable) accession number: Q61672
    Secondary accession number(s): Q9JIT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 8, 2011
    Last modified: June 24, 2015
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.