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Protein

Equilibrative nucleoside transporter 2

Gene

Slc29a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates equilibrative transport of purine and pyrimidine nucleosides, and the purine base hypoxanthine.

Kineticsi

  1. KM=0.75 mM for adenosine

GO - Molecular functioni

  1. nucleoside transmembrane transporter activity Source: MGI

GO - Biological processi

  1. nucleoside transmembrane transport Source: GOC
  2. nucleoside transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Protein family/group databases

TCDBi2.A.57.1.2. the equilibrative nucleoside transporter (ent) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 2
Alternative name(s):
36 kDa hydrophobic nucleolar protein
36 kDa nucleolar protein HNP36
Delayed-early response protein 12
Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter
Short name:
Equilibrative NBMPR-insensitive nucleoside transporter
Nucleoside transporter, ei-type
Solute carrier family 29 member 2
Gene namesi
Name:Slc29a2
Synonyms:Der12, Ent2, Hnp36
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1345278. Slc29a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Transmembranei69 – 8921HelicalSequence AnalysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence AnalysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence AnalysisAdd
BLAST
Transmembranei192 – 21221HelicalSequence AnalysisAdd
BLAST
Transmembranei288 – 30821HelicalSequence AnalysisAdd
BLAST
Transmembranei323 – 34321HelicalSequence AnalysisAdd
BLAST
Transmembranei360 – 38021HelicalSequence AnalysisAdd
BLAST
Transmembranei396 – 41621HelicalSequence AnalysisAdd
BLAST
Transmembranei432 – 45221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB
  2. integral component of plasma membrane Source: InterPro
  3. nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Equilibrative nucleoside transporter 2PRO_0000209341Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Modified residuei252 – 2521PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ61672.
PRIDEiQ61672.

PTM databases

PhosphoSiteiQ61672.

Expressioni

Inductioni

By platelet derived growth factor (PDGF) and fibroblast growth factor (FGF).

Gene expression databases

BgeeiQ61672.
GenevestigatoriQ61672.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025826.

Structurei

3D structure databases

ProteinModelPortaliQ61672.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiQ61672.
KOiK15014.
OMAiFNIMDCL.
OrthoDBiEOG7PP56W.
PhylomeDBiQ61672.
TreeFamiTF313950.

Family and domain databases

InterProiIPR030197. ENT2.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF8. PTHR10332:SF8. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61672-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGNAPRDS YHLVGISFFI LGLGTLLPWN FFITAIPYFQ GRLAGTNSSA
60 70 80 90 100
ETMGTNHTSP TDTFNFNNWV TLLSQLPLLL FTLLNSFLYQ CIPESVRILG
110 120 130 140 150
SLLAILLLFA LTAALVKVDL SPGLFFSVTM ASVWFINSFC AVLQGSLFGQ
160 170 180 190 200
LGTMPSTYST LFLSGQGLAG IFAALAMLMS LASGVDAQTS ALGYFITPCV
210 220 230 240 250
GILLSIVCYL SLPHLKFARY YLTEKLSQAP TQELETKAEL LQADEKNGVP
260 270 280 290 300
ISPQQASPTL DLDPEKEPEP EEPQKPGKPS VFVVFRKIWL TALCLVLVFT
310 320 330 340 350
VTLSVFPAIT AMVTTSSNSP GKWGLFFNPI CCFLLFNVMD WLGRSLTSYF
360 370 380 390 400
LWPDEDSQQL LPLLVCLRFL FVPLFMLCHV PQHARLPIIF RQDAYFITFM
410 420 430 440 450
LLFAVSNGYL VSLTMCLAPR QVLPHEREVA GALMTFFLAL GLSCGASLSF

LFKALL
Length:456
Mass (Da):50,255
Last modified:March 7, 2011 - v2
Checksum:iA9FE7CA037A4468F
GO

Sequence cautioni

The sequence CAA60381.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF183397 mRNA. Translation: AAF78477.1.
CH466612 Genomic DNA. Translation: EDL33088.1.
BC048958 mRNA. Translation: AAH48958.1.
X86682 mRNA. Translation: CAA60381.1. Different initiation.
CCDSiCCDS50354.1.
PIRiJC4195.
RefSeqiNP_031880.2. NM_007854.3.
UniGeneiMm.4930.

Genome annotation databases

EnsembliENSMUST00000025826; ENSMUSP00000025826; ENSMUSG00000024891.
GeneIDi13340.
KEGGimmu:13340.
UCSCiuc008gbv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF183397 mRNA. Translation: AAF78477.1.
CH466612 Genomic DNA. Translation: EDL33088.1.
BC048958 mRNA. Translation: AAH48958.1.
X86682 mRNA. Translation: CAA60381.1. Different initiation.
CCDSiCCDS50354.1.
PIRiJC4195.
RefSeqiNP_031880.2. NM_007854.3.
UniGeneiMm.4930.

3D structure databases

ProteinModelPortaliQ61672.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025826.

Chemistry

ChEMBLiCHEMBL1287612.

Protein family/group databases

TCDBi2.A.57.1.2. the equilibrative nucleoside transporter (ent) family.

PTM databases

PhosphoSiteiQ61672.

Proteomic databases

MaxQBiQ61672.
PRIDEiQ61672.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025826; ENSMUSP00000025826; ENSMUSG00000024891.
GeneIDi13340.
KEGGimmu:13340.
UCSCiuc008gbv.2. mouse.

Organism-specific databases

CTDi3177.
MGIiMGI:1345278. Slc29a2.

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiQ61672.
KOiK15014.
OMAiFNIMDCL.
OrthoDBiEOG7PP56W.
PhylomeDBiQ61672.
TreeFamiTF313950.

Enzyme and pathway databases

ReactomeiREACT_339322. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

NextBioi283654.
PROiQ61672.
SOURCEiSearch...

Gene expression databases

BgeeiQ61672.
GenevestigatoriQ61672.

Family and domain databases

InterProiIPR030197. ENT2.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF8. PTHR10332:SF8. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain."
    Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.
    Biochem. J. 352:363-372(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Brain.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. "A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein."
    Williams J.B., Lanahan A.A.
    Biochem. Biophys. Res. Commun. 213:325-333(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 64-456.
    Strain: BALB/c.
    Tissue: Fibroblast.

Entry informationi

Entry nameiS29A2_MOUSE
AccessioniPrimary (citable) accession number: Q61672
Secondary accession number(s): Q9JIT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: March 7, 2011
Last modified: March 31, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.