ID   HLX_MOUSE               Reviewed;         476 AA.
AC   Q61670;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   24-JAN-2024, entry version 164.
DE   RecName: Full=H2.0-like homeobox protein;
DE   AltName: Full=Homeobox protein HLX1;
GN   Name=Hlx; Synonyms=Hlx1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6 X DBA/2J;
RX   PubMed=1672660; DOI=10.1101/gad.5.4.509;
RA   Allen J.D., Lints T., Jenkins N.A., Copeland N.G., Strasser A.,
RA   Harvey R.P., Adams J.M.;
RT   "Novel murine homeo box gene on chromosome 1 expressed in specific
RT   hematopoietic lineages and during embryogenesis.";
RL   Genes Dev. 5:509-520(1991).
RN   [2]
RP   FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=12055627; DOI=10.1038/ni807;
RA   Mullen A.C., Hutchins A.S., High F.A., Lee H.W., Sykes K.J., Chodosh L.A.,
RA   Reiner S.L.;
RT   "Hlx is induced by and genetically interacts with T-bet to promote
RT   heritable T(H)1 gene induction.";
RL   Nat. Immunol. 3:652-658(2002).
CC   -!- FUNCTION: Transcription factor required for TBX21/T-bet-dependent
CC       maturation of Th1 cells as well as maintenance of Th1-specific gene
CC       expression. Involved in embryogenesis and hematopoiesis.
CC       {ECO:0000269|PubMed:12055627}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in Th1 cells, CD8-positive T-cells, B-
CC       cells and NK cells. {ECO:0000269|PubMed:12055627}.
CC   -!- INDUCTION: By TBX21 in developing as well as mature Th1 cells.
CC       {ECO:0000269|PubMed:12055627}.
CC   -!- DISRUPTION PHENOTYPE: Th1 cells lacking Hlx or Tbx21 fail to express
CC       normal levels of the Th1-specific cytokine Ifng.
CC       {ECO:0000269|PubMed:12055627}.
CC   -!- SIMILARITY: Belongs to the H2.0 homeobox family. {ECO:0000305}.
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DR   EMBL; X58250; CAA41204.1; -; mRNA.
DR   CCDS; CCDS15593.1; -.
DR   PIR; A39621; A39621.
DR   RefSeq; NP_032276.1; NM_008250.2.
DR   AlphaFoldDB; Q61670; -.
DR   SMR; Q61670; -.
DR   STRING; 10090.ENSMUSP00000040505; -.
DR   GlyGen; Q61670; 1 site, 1 O-linked glycan (1 site).
DR   PhosphoSitePlus; Q61670; -.
DR   MaxQB; Q61670; -.
DR   PaxDb; 10090-ENSMUSP00000040505; -.
DR   ProteomicsDB; 273364; -.
DR   Pumba; Q61670; -.
DR   Antibodypedia; 910; 283 antibodies from 32 providers.
DR   DNASU; 15284; -.
DR   Ensembl; ENSMUST00000048572.7; ENSMUSP00000040505.7; ENSMUSG00000039377.8.
DR   GeneID; 15284; -.
DR   KEGG; mmu:15284; -.
DR   UCSC; uc007dyn.2; mouse.
DR   AGR; MGI:96109; -.
DR   CTD; 3142; -.
DR   MGI; MGI:96109; Hlx.
DR   VEuPathDB; HostDB:ENSMUSG00000039377; -.
DR   eggNOG; KOG0488; Eukaryota.
DR   GeneTree; ENSGT00950000183093; -.
DR   HOGENOM; CLU_043671_1_0_1; -.
DR   InParanoid; Q61670; -.
DR   OMA; EPMNEAS; -.
DR   OrthoDB; 2911948at2759; -.
DR   PhylomeDB; Q61670; -.
DR   TreeFam; TF350735; -.
DR   BioGRID-ORCS; 15284; 2 hits in 78 CRISPR screens.
DR   ChiTaRS; Hlx; mouse.
DR   PRO; PR:Q61670; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; Q61670; Protein.
DR   Bgee; ENSMUSG00000039377; Expressed in granulocyte and 162 other cell types or tissues.
DR   ExpressionAtlas; Q61670; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR   GO; GO:0048513; P:animal organ development; IMP:MGI.
DR   GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR   GO; GO:0048557; P:embryonic digestive tract morphogenesis; IMP:MGI.
DR   GO; GO:0048484; P:enteric nervous system development; IMP:MGI.
DR   GO; GO:0050673; P:epithelial cell proliferation; IMP:MGI.
DR   GO; GO:0001889; P:liver development; IMP:MGI.
DR   GO; GO:0045629; P:negative regulation of T-helper 2 cell differentiation; IMP:MGI.
DR   GO; GO:0035265; P:organ growth; IMP:MGI.
DR   GO; GO:0050679; P:positive regulation of epithelial cell proliferation; IMP:MGI.
DR   GO; GO:0046622; P:positive regulation of organ growth; IMP:MGI.
DR   GO; GO:0045627; P:positive regulation of T-helper 1 cell differentiation; IMP:MGI.
DR   GO; GO:0007519; P:skeletal muscle tissue development; IMP:MGI.
DR   GO; GO:0045063; P:T-helper 1 cell differentiation; IMP:MGI.
DR   GO; GO:0045064; P:T-helper 2 cell differentiation; IMP:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR000047; HTH_motif.
DR   PANTHER; PTHR46808; H2.0-LIKE HOMEOBOX PROTEIN; 1.
DR   PANTHER; PTHR46808:SF1; H2.0-LIKE HOMEOBOX PROTEIN; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; Q61670; MM.
PE   2: Evidence at transcript level;
KW   Differentiation; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..476
FT                   /note="H2.0-like homeobox protein"
FT                   /id="PRO_0000048979"
FT   DNA_BIND        273..332
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          120..169
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          328..401
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          413..476
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        132..146
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        328..372
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        413..468
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   476 AA;  50079 MW;  F1C1B5F29218AC45 CRC64;
     MFAAGLAPFY ASNFSLWSAA YCSSAGPGGC SFALDPAAVK KPSFCIADIL HAGVGEPGPA
     AEGLVGASAA LTAHLGSVHP HASFQAAARS PLRPTPVVAP SEVPAGFPQR LSPLSAAFHQ
     HLPQQSPTQQ QQPQQQPPPP PRAVSLQPPT SGTRVVPHHS GSAPAPSSKD LKFGIDRILS
     AEFDPKVKEG NTLRDLTSLL TGGRPAGVHL AGLQPSAGQF FASLDPISEA SAILSPLSSN
     PRNSVQHQFQ DTFPGPYAVL TKDTMPQTYK RKRSWSRAVF SNLQRKGLEK RFEIQKYVTK
     PDRKQLAAML GLTDAQVKVW FQNRRMKWRH SKEAQAQKDK DKEAGEKPSG GVPAEGEREE
     RSPSRSEGEA ESESSDSESL DMAPSDTERT EGTERSLHQT TVIKASAAGA LITASSSTSG
     SSFSFSSTSS LGSGNTHVGS ASSLGGNCSE LPSAHQPSVT SSPQSPEIAQ APLAGL
//