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Protein

Protein HIRA

Gene

Hira

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the periodic repression of histone gene transcription during the cell cycle (By similarity). Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.By similarity1 Publication

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • DNA replication-independent nucleosome assembly Source: MGI
  • gastrulation Source: MGI
  • muscle cell differentiation Source: MGI
  • osteoblast differentiation Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2559584. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HIRA
Alternative name(s):
TUP1-like enhancer of split protein 1
Gene namesi
Name:Hira
Synonyms:Tuple1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:99430. Hira.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • nuclear chromatin Source: MGI
  • nucleus Source: MGI
  • PML body Source: UniProtKB-SubCell
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic stem cells (ES cells) exhibit accelerated differentiation in the early stages which may be attributable to increased availability of soluble histones.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510201 – 1015Protein HIRAAdd BLAST1015

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111PhosphoserineBy similarity1
Modified residuei548PhosphoserineBy similarity1
Modified residuei554Phosphothreonine1 Publication1
Modified residuei556PhosphoserineBy similarity1
Modified residuei575PhosphothreonineBy similarity1
Modified residuei583PhosphoserineBy similarity1
Modified residuei608PhosphoserineBy similarity1
Modified residuei609PhosphoserineBy similarity1
Modified residuei610PhosphoserineBy similarity1
Modified residuei612PhosphoserineBy similarity1
Modified residuei659PhosphoserineBy similarity1
Modified residuei673PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated.By similarity
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-554 in vitro (By similarity). Also phosphorylated on Thr-554 in vivo.By similarity1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61666.
PaxDbiQ61666.
PeptideAtlasiQ61666.
PRIDEiQ61666.

PTM databases

iPTMnetiQ61666.
PhosphoSitePlusiQ61666.

Expressioni

Tissue specificityi

Expressed in cerebrum, cerebellum, heart, kidney, liver, lung and spleen.

Developmental stagei

Throughout development the long isoform is more abundant. In embryos, ubiquitously expressed with high levels detected in cranial neural folds, subregions of pharyngeal arches 1 and 2, circumpharyngeal neural crest and limb buds.2 Publications

Gene expression databases

BgeeiENSMUSG00000022702.
ExpressionAtlasiQ61666. baseline and differential.
GenevisibleiQ61666. MM.

Interactioni

Subunit structurei

Interacts with CCNA1, HIRIP3 and NFU1/HIRIP5. Part of a complex which includes ASF1A, CABIN1, histone H3.3, histone H4 and UBN1 (By similarity). Interacts with histone H2B, histone H3F3B, PAX3 and PAX7.By similarity1 Publication

Protein-protein interaction databases

BioGridi200309. 9 interactors.
IntActiQ61666. 7 interactors.
STRINGi10090.ENSMUSP00000004222.

Structurei

3D structure databases

ProteinModelPortaliQ61666.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 53WD 1Add BLAST43
Repeati68 – 107WD 2Add BLAST40
Repeati129 – 168WD 3Add BLAST40
Repeati172 – 211WD 4Add BLAST40
Repeati220 – 263WD 5Add BLAST44
Repeati266 – 322WD 6Add BLAST57
Repeati326 – 367WD 7Add BLAST42

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni421 – 727Interaction with CCNA1By similarityAdd BLAST307
Regioni421 – 479Interaction with ASF1ABy similarityAdd BLAST59
Regioni439 – 475Required for repression of histone gene transcriptionBy similarityAdd BLAST37
Regioni593 – 737Interaction with PAX31 PublicationAdd BLAST145
Regioni594 – 824Interaction with histone H2BBy similarityAdd BLAST231
Regioni736 – 1015Interaction with histone H4By similarityAdd BLAST280
Regioni738 – 826Interaction with PAX31 PublicationAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi408 – 412Poly-Gln5
Compositional biasi638 – 641Poly-Lys4
Compositional biasi803 – 806Poly-Val4

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0973. Eukaryota.
ENOG410XP1H. LUCA.
GeneTreeiENSGT00550000074919.
HOGENOMiHOG000007670.
HOVERGENiHBG005969.
InParanoidiQ61666.
KOiK11293.
OMAiATREMGS.
OrthoDBiEOG091G04F0.
PhylomeDBiQ61666.
TreeFamiTF323161.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR031120. HIR1.
IPR011494. Hira.
IPR019015. HIRA_B_motif.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13831. PTHR13831. 2 hits.
PfamiPF07569. Hira. 1 hit.
PF09453. HIRA_B. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q61666-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLLKPTWVN HNGKPIFSVD IHPDGTKFAT GGQGQDSGKV VIWNMSPVLQ
60 70 80 90 100
EDDEKDENIP KMLCQMDNHL ACVNCVRWSN SGMYLASGGD DKLIMVWKRA
110 120 130 140 150
TYIGPSTVFG SSGKLANVEQ WRCVSILRSH SGDVMDVAWS PHDAWLASCS
160 170 180 190 200
VDNTVVIWNA VKFPEILATL RGHSGLVKGL TWDPVGKYIA SQADDRSLKV
210 220 230 240 250
WRTLDWQLET SITKPFDECG GTTHVLRLSW SPDGHYLVSA HAMNNSGPTA
260 270 280 290 300
QIIEREGWKT NMDFVGHRKA VTVVKFNPKI FKKKQKNGSS TKPSCPYCCC
310 320 330 340 350
AVGSKDRSLS VWLTCLKRPL VVIHELFDKS IMDISWTLNG LGILVCSMDG
360 370 380 390 400
SVAFLDFSQD ELGDPLSEEE KSRIHQSTYG KSLAIMTEAQ LSTAVIENPE
410 420 430 440 450
MLKYQRRQQQ QQLDQKNATT RETSSASSVT GVVNGESLED IRKNLLKKQV
460 470 480 490 500
ETRTADGRRR ITPLCIAQLD TGDFSTAFFN SIPLSSSLAG TMLSSPSGQQ
510 520 530 540 550
LLPLDSSTPS FGASKPCTEP VAATSARPTG ESVSKDSMNA TSTPAASSPS
560 570 580 590 600
VLTTPSKIEP MKAFDSRFTE RSKATPGAPS LTSVIPTAVE RLKEQNLVKE
610 620 630 640 650
LRSRELESSS DSDEKVHLAK PSSLSKRKLE LEVETVEKKK KGRPRKDSRL
660 670 680 690 700
LPMSLSVQSP AALSTEKEAM CLSAPALALK LPIPGPQRAF TLQVSSDPSM
710 720 730 740 750
YIEVENEVTT VGGIRLSRLK CNREGKEWET VLSSRVLTAA GSCDVVCVAC
760 770 780 790 800
EKRMLSVFST CGRRLLPPIL LPSPISTLHC TGPYVMALTA AATLSVWDVH
810 820 830 840 850
RQVVVVKEES LHSILSGSDM TVSQILLTQH GIPVMNLSDG KAYCFNPSLS
860 870 880 890 900
TWNLVSDKQD SLAQCADFRN SLPSQDAMLC SGPLAIIQGR TSNSGRQAAR
910 920 930 940 950
LFSVPHVVQQ ETTLAYLENQ VAAALTLQSS HEYRHWLLLY ARYLVNEGFE
960 970 980 990 1000
YRLREICKDL LGPVHCSTGS QWESTVVGLR KRELLKELLP VIGQNLRFQR
1010
LFTECQEQLD ILRDK
Length:1,015
Mass (Da):111,767
Last modified:May 1, 2007 - v3
Checksum:i2D84294D45B7A967
GO
Isoform Short (identifier: Q61666-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     556-562: SKIEPMK → PQYIICSP
     563-1015: Missing.

Show »
Length:563
Mass (Da):61,642
Checksum:i7F687DA1201815BD
GO
Isoform 3 (identifier: Q61666-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     473-497: DFSTAFFNSIPLSSSLAGTMLSSPS → YSLLHQAFCGLVWGGLLFFCCQLAS
     498-1015: Missing.

Show »
Length:453
Mass (Da):50,449
Checksum:iFF189AF5E97CF083
GO
Isoform 4 (identifier: Q61666-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-652: Missing.

Note: No experimental confirmation available.
Show »
Length:363
Mass (Da):40,090
Checksum:iFD785BD8473346A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216F → C in CAA63334 (PubMed:8611624).Curated1
Sequence conflicti400E → G in AAH52856 (PubMed:15489334).Curated1
Sequence conflicti459R → S in CAA63334 (PubMed:8611624).Curated1
Sequence conflicti650L → F in CAA63334 (PubMed:8611624).Curated1
Sequence conflicti712G → R in CAA68049 (PubMed:9063745).Curated1
Sequence conflicti1007E → G in CAA63334 (PubMed:8611624).Curated1
Sequence conflicti1013 – 1014RD → WG in CAA63334 (PubMed:8611624).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0250491 – 652Missing in isoform 4. 1 PublicationAdd BLAST652
Alternative sequenceiVSP_0250501 – 44Missing in isoform 3. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_025051473 – 497DFSTA…LSSPS → YSLLHQAFCGLVWGGLLFFC CQLAS in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_025052498 – 1015Missing in isoform 3. 1 PublicationAdd BLAST518
Alternative sequenceiVSP_006773556 – 562SKIEPMK → PQYIICSP in isoform Short. 1 Publication7
Alternative sequenceiVSP_006774563 – 1015Missing in isoform Short. 1 PublicationAdd BLAST453

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92590 mRNA. Translation: CAA63334.1.
X99712 mRNA. Translation: CAA68049.1.
X75295 mRNA. Translation: CAA53043.1.
AK135928 mRNA. Translation: BAE22728.1.
AK168963 mRNA. Translation: BAE40768.1.
BC052856 mRNA. Translation: AAH52856.1.
CCDSiCCDS28031.1. [Q61666-1]
PIRiS45345.
S68141.
RefSeqiNP_034565.2. NM_010435.2. [Q61666-1]
UniGeneiMm.15694.
Mm.453041.

Genome annotation databases

EnsembliENSMUST00000004222; ENSMUSP00000004222; ENSMUSG00000022702. [Q61666-1]
ENSMUST00000120532; ENSMUSP00000112614; ENSMUSG00000022702. [Q61666-3]
GeneIDi15260.
KEGGimmu:15260.
UCSCiuc007yoq.1. mouse. [Q61666-1]
uc007yor.1. mouse. [Q61666-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92590 mRNA. Translation: CAA63334.1.
X99712 mRNA. Translation: CAA68049.1.
X75295 mRNA. Translation: CAA53043.1.
AK135928 mRNA. Translation: BAE22728.1.
AK168963 mRNA. Translation: BAE40768.1.
BC052856 mRNA. Translation: AAH52856.1.
CCDSiCCDS28031.1. [Q61666-1]
PIRiS45345.
S68141.
RefSeqiNP_034565.2. NM_010435.2. [Q61666-1]
UniGeneiMm.15694.
Mm.453041.

3D structure databases

ProteinModelPortaliQ61666.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200309. 9 interactors.
IntActiQ61666. 7 interactors.
STRINGi10090.ENSMUSP00000004222.

PTM databases

iPTMnetiQ61666.
PhosphoSitePlusiQ61666.

Proteomic databases

EPDiQ61666.
PaxDbiQ61666.
PeptideAtlasiQ61666.
PRIDEiQ61666.

Protocols and materials databases

DNASUi15260.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004222; ENSMUSP00000004222; ENSMUSG00000022702. [Q61666-1]
ENSMUST00000120532; ENSMUSP00000112614; ENSMUSG00000022702. [Q61666-3]
GeneIDi15260.
KEGGimmu:15260.
UCSCiuc007yoq.1. mouse. [Q61666-1]
uc007yor.1. mouse. [Q61666-3]

Organism-specific databases

CTDi7290.
MGIiMGI:99430. Hira.

Phylogenomic databases

eggNOGiKOG0973. Eukaryota.
ENOG410XP1H. LUCA.
GeneTreeiENSGT00550000074919.
HOGENOMiHOG000007670.
HOVERGENiHBG005969.
InParanoidiQ61666.
KOiK11293.
OMAiATREMGS.
OrthoDBiEOG091G04F0.
PhylomeDBiQ61666.
TreeFamiTF323161.

Enzyme and pathway databases

ReactomeiR-MMU-2559584. Formation of Senescence-Associated Heterochromatin Foci (SAHF).

Miscellaneous databases

PROiQ61666.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022702.
ExpressionAtlasiQ61666. baseline and differential.
GenevisibleiQ61666. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR031120. HIR1.
IPR011494. Hira.
IPR019015. HIRA_B_motif.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13831. PTHR13831. 2 hits.
PfamiPF07569. Hira. 1 hit.
PF09453. HIRA_B. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIRA_MOUSE
AccessioniPrimary (citable) accession number: Q61666
Secondary accession number(s): O08845
, Q3TFY0, Q3UX35, Q62365, Q7TMW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.