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Q61666 (HIRA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein HIRA
Alternative name(s):
TUP1-like enhancer of split protein 1
Gene names
Name:Hira
Synonyms:Tuple1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1015 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the periodic repression of histone gene transcription during the cell cycle By similarity. Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. Ref.9

Subunit structure

Interacts with CCNA1, HIRIP3 and NFU1/HIRIP5. Part of a complex which includes ASF1A, CABIN1, histone H3.3, histone H4 and UBN1 By similarity. Interacts with histone H2B, histone H3F3B, PAX3 and PAX7. Ref.6

Subcellular location

Nucleus By similarity. NucleusPML body By similarity. Note: Primarily, though not exclusively, localized to the nucleus By similarity. Localizes to PML bodies immediately prior to onset of senescence By similarity. Localizes specifically to the male nucleus in fertilized eggs. This localization persists from the initiation of sperm nucleus decondensation to pronucleus formation. Ref.6 Ref.8

Tissue specificity

Expressed in cerebrum, cerebellum, heart, kidney, liver, lung and spleen.

Developmental stage

Throughout development the long isoform ismore abundant. In embryos, ubiquitously expressed with high levels detected in cranial neural folds, subregions of pharyngeal arches 1 and 2, circumpharyngeal neural crest and limb buds. Ref.2 Ref.6

Post-translational modification

Sumoylated By similarity.

Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-554 in vitro By similarity. Also phosphorylated on Thr-554 in vivo. Ref.7

Disruption phenotype

Embryonic stem cells (ES cells) exhibit accelerated differentiation in the early stages which may be attributable to increased availability of soluble histones. Ref.9

Sequence similarities

Belongs to the WD repeat HIR1 family.

Contains 7 WD repeats.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q61666-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q61666-2)

The sequence of this isoform differs from the canonical sequence as follows:
     556-562: SKIEPMK → PQYIICSP
     563-1015: Missing.
Isoform 3 (identifier: Q61666-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     473-497: DFSTAFFNSIPLSSSLAGTMLSSPS → YSLLHQAFCGLVWGGLLFFCCQLAS
     498-1015: Missing.
Isoform 4 (identifier: Q61666-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-652: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10151015Protein HIRA
PRO_0000051020

Regions

Repeat11 – 5343WD 1
Repeat68 – 10740WD 2
Repeat129 – 16840WD 3
Repeat172 – 21140WD 4
Repeat220 – 26344WD 5
Repeat266 – 32257WD 6
Repeat326 – 36742WD 7
Region421 – 727307Interaction with CCNA1 By similarity
Region421 – 47959Interaction with ASF1A By similarity
Region439 – 47537Required for repression of histone gene transcription By similarity
Region593 – 737145Interaction with PAX3
Region594 – 824231Interaction with histone H2B By similarity
Region736 – 1015280Interaction with histone H4 By similarity
Region738 – 82689Interaction with PAX3
Compositional bias408 – 4125Poly-Gln
Compositional bias638 – 6414Poly-Lys
Compositional bias803 – 8064Poly-Val

Amino acid modifications

Modified residue5481Phosphoserine By similarity
Modified residue5541Phosphothreonine Ref.7
Modified residue5561Phosphoserine By similarity
Modified residue5751Phosphothreonine By similarity
Modified residue5831Phosphoserine By similarity
Modified residue6081Phosphoserine By similarity
Modified residue6091Phosphoserine By similarity
Modified residue6101Phosphoserine By similarity
Modified residue6121Phosphoserine By similarity
Modified residue6591Phosphoserine By similarity

Natural variations

Alternative sequence1 – 652652Missing in isoform 4.
VSP_025049
Alternative sequence1 – 4444Missing in isoform 3.
VSP_025050
Alternative sequence473 – 49725DFSTA…LSSPS → YSLLHQAFCGLVWGGLLFFC CQLAS in isoform 3.
VSP_025051
Alternative sequence498 – 1015518Missing in isoform 3.
VSP_025052
Alternative sequence556 – 5627SKIEPMK → PQYIICSP in isoform Short.
VSP_006773
Alternative sequence563 – 1015453Missing in isoform Short.
VSP_006774

Experimental info

Sequence conflict2161F → C in CAA63334. Ref.1
Sequence conflict4001E → G in AAH52856. Ref.5
Sequence conflict4591R → S in CAA63334. Ref.1
Sequence conflict6501L → F in CAA63334. Ref.1
Sequence conflict7121G → R in CAA68049. Ref.2
Sequence conflict10071E → G in CAA63334. Ref.1
Sequence conflict1013 – 10142RD → WG in CAA63334. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 1, 2007. Version 3.
Checksum: 2D84294D45B7A967

FASTA1,015111,767
        10         20         30         40         50         60 
MKLLKPTWVN HNGKPIFSVD IHPDGTKFAT GGQGQDSGKV VIWNMSPVLQ EDDEKDENIP 

        70         80         90        100        110        120 
KMLCQMDNHL ACVNCVRWSN SGMYLASGGD DKLIMVWKRA TYIGPSTVFG SSGKLANVEQ 

       130        140        150        160        170        180 
WRCVSILRSH SGDVMDVAWS PHDAWLASCS VDNTVVIWNA VKFPEILATL RGHSGLVKGL 

       190        200        210        220        230        240 
TWDPVGKYIA SQADDRSLKV WRTLDWQLET SITKPFDECG GTTHVLRLSW SPDGHYLVSA 

       250        260        270        280        290        300 
HAMNNSGPTA QIIEREGWKT NMDFVGHRKA VTVVKFNPKI FKKKQKNGSS TKPSCPYCCC 

       310        320        330        340        350        360 
AVGSKDRSLS VWLTCLKRPL VVIHELFDKS IMDISWTLNG LGILVCSMDG SVAFLDFSQD 

       370        380        390        400        410        420 
ELGDPLSEEE KSRIHQSTYG KSLAIMTEAQ LSTAVIENPE MLKYQRRQQQ QQLDQKNATT 

       430        440        450        460        470        480 
RETSSASSVT GVVNGESLED IRKNLLKKQV ETRTADGRRR ITPLCIAQLD TGDFSTAFFN 

       490        500        510        520        530        540 
SIPLSSSLAG TMLSSPSGQQ LLPLDSSTPS FGASKPCTEP VAATSARPTG ESVSKDSMNA 

       550        560        570        580        590        600 
TSTPAASSPS VLTTPSKIEP MKAFDSRFTE RSKATPGAPS LTSVIPTAVE RLKEQNLVKE 

       610        620        630        640        650        660 
LRSRELESSS DSDEKVHLAK PSSLSKRKLE LEVETVEKKK KGRPRKDSRL LPMSLSVQSP 

       670        680        690        700        710        720 
AALSTEKEAM CLSAPALALK LPIPGPQRAF TLQVSSDPSM YIEVENEVTT VGGIRLSRLK 

       730        740        750        760        770        780 
CNREGKEWET VLSSRVLTAA GSCDVVCVAC EKRMLSVFST CGRRLLPPIL LPSPISTLHC 

       790        800        810        820        830        840 
TGPYVMALTA AATLSVWDVH RQVVVVKEES LHSILSGSDM TVSQILLTQH GIPVMNLSDG 

       850        860        870        880        890        900 
KAYCFNPSLS TWNLVSDKQD SLAQCADFRN SLPSQDAMLC SGPLAIIQGR TSNSGRQAAR 

       910        920        930        940        950        960 
LFSVPHVVQQ ETTLAYLENQ VAAALTLQSS HEYRHWLLLY ARYLVNEGFE YRLREICKDL 

       970        980        990       1000       1010 
LGPVHCSTGS QWESTVVGLR KRELLKELLP VIGQNLRFQR LFTECQEQLD ILRDK 

« Hide

Isoform Short [UniParc].

Checksum: 7F687DA1201815BD
Show »

FASTA56361,642
Isoform 3 [UniParc].

Checksum: FF189AF5E97CF083
Show »

FASTA45350,449
Isoform 4 [UniParc].

Checksum: FD785BD8473346A1
Show »

FASTA36340,090

References

« Hide 'large scale' references
[1]"The HIR protein family: isolation and characterization of a complete murine cDNA."
Scamps C., Lorain S., Lamour V., Lipinski M.
Biochim. Biophys. Acta 1306:5-8(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
Strain: DBA/2.
[2]"The murine homologue of HIRA, a DiGeorge syndrome candidate gene, is expressed in embryonic structures affected in human CATCH22 patients."
Wilming L.G., Snoeren C.A.S., van Rijswijk A., Grosveld F., Meijers C.
Hum. Mol. Genet. 6:247-258(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), DEVELOPMENTAL STAGE.
Strain: ICR X Swiss Webster.
Tissue: Embryo.
[3]"Isolation of a putative transcriptional regulator from the region of 22q11 deleted in DiGeorge syndrome, Shprintzen syndrome and familial congenital heart disease."
Halford S., Wadey R., Roberts C., Daw S.C.M., Whiting J.A., O'Donnell H., Dunham I., Bentley D., Lindsay E., Baldini A., Francis F., Lehrach H., Williamson R., Wilson D.I., Goodship J., Cross I., Burn J., Scambler P.J.
Hum. Mol. Genet. 2:2099-2107(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-562 (ISOFORM SHORT).
Strain: BALB/c and C57BL/6J.
Tissue: Embryo and Kidney.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 748-1015 (ISOFORMS LONG/4).
Strain: C57BL/6J.
Tissue: Egg.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: C3H/He.
Tissue: Mesenchymal stem cell.
[6]"HIRA, a mammalian homologue of Saccharomyces cerevisiae transcriptional co-repressors, interacts with Pax3."
Magnaghi P., Roberts C., Lorain S., Lipinski M., Scambler P.J.
Nat. Genet. 20:74-77(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HISTONE H2B; HISTONE H3F3B; PAX3 AND PAX7, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[7]"HIRA, the human homologue of yeast Hir1p and Hir2p, is a novel cyclin-cdk2 substrate whose expression blocks S-phase progression."
Hall C., Nelson D.M., Ye X., Baker K., DeCaprio J.A., Seeholzer S., Lipinski M., Adams P.D.
Mol. Cell. Biol. 21:1854-1865(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-554.
[8]"Asymmetry in histone H3 variants and lysine methylation between paternal and maternal chromatin of the early mouse zygote."
van der Heijden G.W., Dieker J.W., Derijck A.A.H.A., Muller S., Berden J.H.M., Braat D.D.M., van der Vlag J., de Boer P.
Mech. Dev. 122:1008-1022(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells."
Meshorer E., Yellajoshula D., George E., Scambler P.J., Brown D.T., Misteli T.
Dev. Cell 10:105-116(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92590 mRNA. Translation: CAA63334.1.
X99712 mRNA. Translation: CAA68049.1.
X75295 mRNA. Translation: CAA53043.1.
AK135928 mRNA. Translation: BAE22728.1.
AK168963 mRNA. Translation: BAE40768.1.
BC052856 mRNA. Translation: AAH52856.1.
CCDSCCDS28031.1. [Q61666-1]
PIRS45345.
S68141.
RefSeqNP_034565.2. NM_010435.2. [Q61666-1]
UniGeneMm.15694.
Mm.453041.

3D structure databases

ProteinModelPortalQ61666.
SMRQ61666. Positions 10-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200309. 8 interactions.
IntActQ61666. 7 interactions.
STRING10090.ENSMUSP00000004222.

PTM databases

PhosphoSiteQ61666.

Proteomic databases

MaxQBQ61666.
PRIDEQ61666.

Protocols and materials databases

DNASU15260.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004222; ENSMUSP00000004222; ENSMUSG00000022702. [Q61666-1]
ENSMUST00000120532; ENSMUSP00000112614; ENSMUSG00000022702. [Q61666-3]
GeneID15260.
KEGGmmu:15260.
UCSCuc007yoq.1. mouse. [Q61666-1]
uc007yor.1. mouse. [Q61666-3]

Organism-specific databases

CTD7290.
MGIMGI:99430. Hira.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00550000074919.
HOGENOMHOG000007670.
HOVERGENHBG005969.
InParanoidQ61666.
KOK11293.
OMAAVTNPCM.
OrthoDBEOG74J975.
PhylomeDBQ61666.
TreeFamTF323161.

Gene expression databases

ArrayExpressQ61666.
BgeeQ61666.
GenevestigatorQ61666.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR011494. Hira.
IPR019015. HIRA_B_motif.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF07569. Hira. 1 hit.
PF09453. HIRA_B. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287885.
PROQ61666.
SOURCESearch...

Entry information

Entry nameHIRA_MOUSE
AccessionPrimary (citable) accession number: Q61666
Secondary accession number(s): O08845 expand/collapse secondary AC list , Q3TFY0, Q3UX35, Q62365, Q7TMW4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 2007
Last modified: July 9, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot