ID   HESX1_MOUSE             Reviewed;         185 AA.
AC   Q61658; Q61047; Q8C477;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 2.
DT   24-JAN-2024, entry version 179.
DE   RecName: Full=Homeobox expressed in ES cells 1;
DE   AltName: Full=Anterior-restricted homeobox protein;
DE   AltName: Full=Homeobox protein ANF;
DE   AltName: Full=Rathke pouch homeo box;
GN   Name=Hesx1; Synonyms=Hes-1, Rpx;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=129/Sv; TISSUE=Embryonic stem cell;
RX   PubMed=7876132; DOI=10.1074/jbc.270.8.3869;
RA   Thomas P.Q., Johnson B.V., Rathjen J., Rathjen P.D.;
RT   "Sequence, genomic organization, and expression of the novel homeobox gene
RT   Hesx1.";
RL   J. Biol. Chem. 270:3869-3875(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Embryo;
RX   PubMed=8565852; DOI=10.1242/dev.122.1.41;
RA   Hermesz E., Mackem S., Mahon K.A.;
RT   "Rpx: a novel anterior-restricted homeobox gene progressively activated in
RT   the prechordal plate, anterior neural plate and Rathke's pouch of the mouse
RT   embryo.";
RL   Development 122:41-52(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=1360650; DOI=10.1093/nar/20.21.5840;
RA   Thomas P.Q., Rathjen P.D.;
RT   "HES-1, a novel homeobox gene expressed by murine embryonic stem cells,
RT   identifies a new class of homeobox genes.";
RL   Nucleic Acids Res. 20:5840-5840(1992).
RN   [7]
RP   FUNCTION.
RX   PubMed=9620767; DOI=10.1038/477;
RA   Dattani M.T., Martinez-Barbera J.-P., Thomas P.Q., Brickman J.M., Gupta R.,
RA   Maartensson I.-L., Toresson H., Fox M., Wales J.K.H., Hindmarsh P.C.,
RA   Krauss S., Beddington R.S.P., Robinson I.C.A.F.;
RT   "Mutations in the homeobox gene HESX1/Hesx1 associated with septo-optic
RT   dysplasia in human and mouse.";
RL   Nat. Genet. 19:125-133(1998).
CC   -!- FUNCTION: Required for the normal development of the forebrain, eyes
CC       and other anterior structures such as the olfactory placodes and
CC       pituitary gland. Possible transcriptional repressor. Binds to the
CC       palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1
CC       and PROP1 bind as heterodimers on this palindromic site, and, in vitro,
CC       HESX1 can antagonize PROP1 activation. {ECO:0000269|PubMed:9620767}.
CC   -!- SUBUNIT: Can form heterodimers with PROP1 in binding to DNA Interacts
CC       with TLE1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: High levels found in the embryonic liver, lower
CC       level expression seen in the viscera, amnion and yolk sac.
CC   -!- DEVELOPMENTAL STAGE: Early expression seen in the anterior midline
CC       endoderm and prechordal plate precursor, subsequently activated in the
CC       overlying ectoderm of the cephalic neural plate. Later disappears in
CC       the mesendoderm while remaining in the prospective prosencephalic
CC       region of the neural plate ectoderm, ultimately expression is
CC       restricted to Rathke pouch, the primordium of the pituitary. Expressed
CC       in embryonic stem cells. {ECO:0000269|PubMed:1360650}.
CC   -!- INDUCTION: Down-regulated during embryonic stem (ES) cell
CC       differentiation.
CC   -!- SIMILARITY: Belongs to the ANF homeobox family. {ECO:0000305}.
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DR   EMBL; X80040; CAA56344.1; -; mRNA.
DR   EMBL; U40720; AAA97869.1; -; mRNA.
DR   EMBL; AK082831; BAC38643.1; -; mRNA.
DR   EMBL; AC124603; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC132051; AAI32052.1; -; mRNA.
DR   EMBL; BC132053; AAI32054.1; -; mRNA.
DR   EMBL; BC145262; AAI45263.1; -; mRNA.
DR   CCDS; CCDS26884.1; -.
DR   PIR; A55882; A55882.
DR   RefSeq; NP_034550.2; NM_010420.2.
DR   RefSeq; XP_006518633.1; XM_006518570.3.
DR   AlphaFoldDB; Q61658; -.
DR   SMR; Q61658; -.
DR   BioGRID; 200279; 5.
DR   STRING; 10090.ENSMUSP00000041999; -.
DR   PhosphoSitePlus; Q61658; -.
DR   PaxDb; 10090-ENSMUSP00000041999; -.
DR   Antibodypedia; 14999; 105 antibodies from 21 providers.
DR   DNASU; 15209; -.
DR   Ensembl; ENSMUST00000035433.10; ENSMUSP00000041999.8; ENSMUSG00000040726.11.
DR   Ensembl; ENSMUST00000224331.2; ENSMUSP00000152952.2; ENSMUSG00000040726.11.
DR   GeneID; 15209; -.
DR   KEGG; mmu:15209; -.
DR   UCSC; uc007stj.1; mouse.
DR   AGR; MGI:96071; -.
DR   CTD; 8820; -.
DR   MGI; MGI:96071; Hesx1.
DR   VEuPathDB; HostDB:ENSMUSG00000040726; -.
DR   eggNOG; KOG0490; Eukaryota.
DR   GeneTree; ENSGT00940000156780; -.
DR   HOGENOM; CLU_125528_0_0_1; -.
DR   InParanoid; Q61658; -.
DR   OMA; FHARSDP; -.
DR   OrthoDB; 5401018at2759; -.
DR   PhylomeDB; Q61658; -.
DR   TreeFam; TF335506; -.
DR   BioGRID-ORCS; 15209; 0 hits in 81 CRISPR screens.
DR   PRO; PR:Q61658; -.
DR   Proteomes; UP000000589; Chromosome 14.
DR   RNAct; Q61658; Protein.
DR   Bgee; ENSMUSG00000040726; Expressed in remnant of Rathke's pouch and 47 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; ISO:MGI.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:MGI.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:MGI.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IMP:MGI.
DR   GO; GO:0007420; P:brain development; IMP:MGI.
DR   GO; GO:0043010; P:camera-type eye development; IMP:MGI.
DR   GO; GO:0060070; P:canonical Wnt signaling pathway; IMP:MGI.
DR   GO; GO:0071276; P:cellular response to cadmium ion; IDA:MGI.
DR   GO; GO:0070371; P:ERK1 and ERK2 cascade; IMP:MGI.
DR   GO; GO:0030900; P:forebrain development; IDA:MGI.
DR   GO; GO:0048853; P:forebrain morphogenesis; IMP:MGI.
DR   GO; GO:0010467; P:gene expression; IDA:MGI.
DR   GO; GO:0008406; P:gonad development; IGI:MGI.
DR   GO; GO:0048861; P:leukemia inhibitory factor signaling pathway; IMP:MGI.
DR   GO; GO:0035264; P:multicellular organism growth; IGI:MGI.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IDA:MGI.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0043584; P:nose development; IMP:MGI.
DR   GO; GO:0030916; P:otic vesicle formation; IMP:MGI.
DR   GO; GO:0021983; P:pituitary gland development; IMP:MGI.
DR   GO; GO:0045995; P:regulation of embryonic development; IMP:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0048863; P:stem cell differentiation; IMP:MGI.
DR   GO; GO:0019827; P:stem cell population maintenance; IMP:MGI.
DR   GO; GO:0030878; P:thyroid gland development; IGI:MGI.
DR   GO; GO:0016055; P:Wnt signaling pathway; IDA:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR043402; Hesx1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   PANTHER; PTHR46966; HOMEOBOX EXPRESSED IN ES CELLS 1; 1.
DR   PANTHER; PTHR46966:SF1; HOMEOBOX EXPRESSED IN ES CELLS 1; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; Q61658; MM.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..185
FT                   /note="Homeobox expressed in ES cells 1"
FT                   /id="PRO_0000048923"
FT   DNA_BIND        108..167
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          32..69
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        51
FT                   /note="D -> N (in Ref. 1; CAA56344 and 2; AAA97869)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        55
FT                   /note="G -> D (in Ref. 1; CAA56344 and 2; AAA97869)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        176
FT                   /note="K -> R (in Ref. 1; CAA56344)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   185 AA;  21504 MW;  5C18A524BF281246 CRC64;
     MSPSLREGAQ LRESKPAPCS FSIESILGLD QKKDCTTSVR PHRPWTDTCG DSEKGGNPPL
     HAPDLPSETS FPCPVDHPRP EERAPKYENY FSASETRSLK RELSWYRGRR PRTAFTQNQV
     EVLENVFRVN CYPGIDIRED LAQKLNLEED RIQIWFQNRR AKMKRSRRES QFLMAKKPFN
     PDLLK
//