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Q61658 (HESX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox expressed in ES cells 1
Alternative name(s):
Anterior-restricted homeobox protein
Homeobox protein ANF
Rathke pouch homeo box
Gene names
Name:Hesx1
Synonyms:Hes-1, Rpx
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation. Ref.7

Subunit structure

Can form heterodimers with PROP1 in binding to DNA Interacts with TLE1 By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

High levels found in the embryonic liver, lower level expression seen in the viscera, amnion and yolk sac.

Developmental stage

Early expression seen in the anterior midline endoderm and prechordal plate precursor, subsequently activated in the overlying ectoderm of the cephalic neural plate. Later disappears in the mesendoderm while remaining in the prospective prosencephalic region of the neural plate ectoderm, ultimately expression is restricted to Rathke pouch, the primordium of the pituitary. Expressed in embryonic stem cells. Ref.6

Induction

Down-regulated during embryonic stem (ES) cell differentiation.

Sequence similarities

Belongs to the ANF homeobox family.

Contains 1 homeobox DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185Homeobox expressed in ES cells 1
PRO_0000048923

Regions

DNA binding108 – 16760Homeobox

Experimental info

Sequence conflict511D → N in CAA56344. Ref.1
Sequence conflict511D → N in AAA97869. Ref.2
Sequence conflict551G → D in CAA56344. Ref.1
Sequence conflict551G → D in AAA97869. Ref.2
Sequence conflict1761K → R in CAA56344. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61658 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 5C18A524BF281246

FASTA18521,504
        10         20         30         40         50         60 
MSPSLREGAQ LRESKPAPCS FSIESILGLD QKKDCTTSVR PHRPWTDTCG DSEKGGNPPL 

        70         80         90        100        110        120 
HAPDLPSETS FPCPVDHPRP EERAPKYENY FSASETRSLK RELSWYRGRR PRTAFTQNQV 

       130        140        150        160        170        180 
EVLENVFRVN CYPGIDIRED LAQKLNLEED RIQIWFQNRR AKMKRSRRES QFLMAKKPFN 


PDLLK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence, genomic organization, and expression of the novel homeobox gene Hesx1."
Thomas P.Q., Johnson B.V., Rathjen J., Rathjen P.D.
J. Biol. Chem. 270:3869-3875(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
Tissue: Embryonic stem cell.
[2]"Rpx: a novel anterior-restricted homeobox gene progressively activated in the prechordal plate, anterior neural plate and Rathke's pouch of the mouse embryo."
Hermesz E., Mackem S., Mahon K.A.
Development 122:41-52(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"HES-1, a novel homeobox gene expressed by murine embryonic stem cells, identifies a new class of homeobox genes."
Thomas P.Q., Rathjen P.D.
Nucleic Acids Res. 20:5840-5840(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[7]"Mutations in the homeobox gene HESX1/Hesx1 associated with septo-optic dysplasia in human and mouse."
Dattani M.T., Martinez-Barbera J.-P., Thomas P.Q., Brickman J.M., Gupta R., Maartensson I.-L., Toresson H., Fox M., Wales J.K.H., Hindmarsh P.C., Krauss S., Beddington R.S.P., Robinson I.C.A.F.
Nat. Genet. 19:125-133(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80040 mRNA. Translation: CAA56344.1.
U40720 mRNA. Translation: AAA97869.1.
AK082831 mRNA. Translation: BAC38643.1.
AC124603 Genomic DNA. No translation available.
BC132051 mRNA. Translation: AAI32052.1.
BC132053 mRNA. Translation: AAI32054.1.
BC145262 mRNA. Translation: AAI45263.1.
PIRA55882.
RefSeqNP_034550.2. NM_010420.2.
XP_006518633.1. XM_006518570.1.
UniGeneMm.4802.

3D structure databases

ProteinModelPortalQ61658.
SMRQ61658. Positions 108-174.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200279. 5 interactions.
STRING10090.ENSMUSP00000041999.

Proteomic databases

PRIDEQ61658.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035433; ENSMUSP00000041999; ENSMUSG00000040726.
GeneID15209.
KEGGmmu:15209.
UCSCuc007stj.1. mouse.

Organism-specific databases

CTD8820.
MGIMGI:96071. Hesx1.

Phylogenomic databases

eggNOGNOG326168.
GeneTreeENSGT00750000117223.
HOGENOMHOG000112889.
HOVERGENHBG004226.
InParanoidQ61658.
KOK09354.
OMAHPMPEER.
OrthoDBEOG7WMCMW.
TreeFamTF335506.

Gene expression databases

CleanExMM_HESX1.
GenevestigatorQ61658.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287773.
PROQ61658.
SOURCESearch...

Entry information

Entry nameHESX1_MOUSE
AccessionPrimary (citable) accession number: Q61658
Secondary accession number(s): Q61047, Q8C477
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot