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Protein

Homeobox expressed in ES cells 1

Gene

Hesx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi108 – 167HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • protein C-terminus binding Source: MGI
  • protein N-terminus binding Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • brain development Source: MGI
  • forebrain morphogenesis Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • nose development Source: MGI
  • otic vesicle formation Source: MGI
  • pituitary gland development Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox expressed in ES cells 1
Alternative name(s):
Anterior-restricted homeobox protein
Homeobox protein ANF
Rathke pouch homeo box
Gene namesi
Name:Hesx1
Synonyms:Hes-1, Rpx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:96071. Hesx1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000489231 – 185Homeobox expressed in ES cells 1Add BLAST185

Proteomic databases

PaxDbiQ61658.
PRIDEiQ61658.

PTM databases

PhosphoSitePlusiQ61658.

Expressioni

Tissue specificityi

High levels found in the embryonic liver, lower level expression seen in the viscera, amnion and yolk sac.

Developmental stagei

Early expression seen in the anterior midline endoderm and prechordal plate precursor, subsequently activated in the overlying ectoderm of the cephalic neural plate. Later disappears in the mesendoderm while remaining in the prospective prosencephalic region of the neural plate ectoderm, ultimately expression is restricted to Rathke pouch, the primordium of the pituitary. Expressed in embryonic stem cells.1 Publication

Inductioni

Down-regulated during embryonic stem (ES) cell differentiation.

Gene expression databases

BgeeiENSMUSG00000040726.
CleanExiMM_HESX1.
GenevisibleiQ61658. MM.

Interactioni

Subunit structurei

Can form heterodimers with PROP1 in binding to DNA Interacts with TLE1.By similarity

GO - Molecular functioni

  • protein C-terminus binding Source: MGI
  • protein N-terminus binding Source: MGI
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi200279. 5 interactors.
STRINGi10090.ENSMUSP00000041999.

Structurei

3D structure databases

ProteinModelPortaliQ61658.
SMRiQ61658.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ANF homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00860000133740.
HOGENOMiHOG000112889.
HOVERGENiHBG004226.
InParanoidiQ61658.
KOiK09354.
OMAiKPSTCSF.
OrthoDBiEOG091G112W.
TreeFamiTF335506.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like_sf.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPSLREGAQ LRESKPAPCS FSIESILGLD QKKDCTTSVR PHRPWTDTCG
60 70 80 90 100
DSEKGGNPPL HAPDLPSETS FPCPVDHPRP EERAPKYENY FSASETRSLK
110 120 130 140 150
RELSWYRGRR PRTAFTQNQV EVLENVFRVN CYPGIDIRED LAQKLNLEED
160 170 180
RIQIWFQNRR AKMKRSRRES QFLMAKKPFN PDLLK
Length:185
Mass (Da):21,504
Last modified:October 3, 2012 - v2
Checksum:i5C18A524BF281246
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51D → N in CAA56344 (PubMed:7876132).Curated1
Sequence conflicti51D → N in AAA97869 (PubMed:8565852).Curated1
Sequence conflicti55G → D in CAA56344 (PubMed:7876132).Curated1
Sequence conflicti55G → D in AAA97869 (PubMed:8565852).Curated1
Sequence conflicti176K → R in CAA56344 (PubMed:7876132).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80040 mRNA. Translation: CAA56344.1.
U40720 mRNA. Translation: AAA97869.1.
AK082831 mRNA. Translation: BAC38643.1.
AC124603 Genomic DNA. No translation available.
BC132051 mRNA. Translation: AAI32052.1.
BC132053 mRNA. Translation: AAI32054.1.
BC145262 mRNA. Translation: AAI45263.1.
CCDSiCCDS26884.1.
PIRiA55882.
RefSeqiNP_034550.2. NM_010420.2.
XP_006518633.1. XM_006518570.3.
UniGeneiMm.4802.

Genome annotation databases

EnsembliENSMUST00000035433; ENSMUSP00000041999; ENSMUSG00000040726.
ENSMUST00000224331; ENSMUSP00000152952; ENSMUSG00000040726.
GeneIDi15209.
KEGGimmu:15209.
UCSCiuc007stj.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiHESX1_MOUSE
AccessioniPrimary (citable) accession number: Q61658
Secondary accession number(s): Q61047, Q8C477
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 3, 2012
Last modified: November 22, 2017
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families