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Protein

Probable ATP-dependent RNA helicase DDX5

Gene

Ddx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi114 – 116ATPPROSITE-ProRule annotation3
Nucleotide bindingi138 – 145ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Biological rhythms, mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi3.6.4.13. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX5 (EC:3.6.4.13)
Alternative name(s):
DEAD box RNA helicase DEAD1
Short name:
mDEAD1
DEAD box protein 5
RNA helicase p68
Gene namesi
Name:Ddx5
Synonyms:Tnz2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105037. Ddx5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549921 – 614Probable ATP-dependent RNA helicase DDX5Add BLAST614

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Modified residuei32N6-acetyllysineBy similarity1
Modified residuei33N6-acetyllysineBy similarity1
Modified residuei40N6-acetyllysineCombined sources1
Cross-linki53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei236N6-acetyllysineCombined sources1
Modified residuei297PhosphotyrosineBy similarity1
Modified residuei480PhosphoserineBy similarity1
Modified residuei502Omega-N-methylated arginineBy similarity1

Post-translational modificationi

Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination (By similarity).By similarity
Polyubiquitinated, leading to proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61656.
MaxQBiQ61656.
PaxDbiQ61656.
PRIDEiQ61656.

PTM databases

iPTMnetiQ61656.
PhosphoSitePlusiQ61656.
SwissPalmiQ61656.

Expressioni

Gene expression databases

CleanExiMM_DDX5.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with RBM4; the interaction occurs in an RNA-independent manner. Interacts with AGO1 and AGO2. Interacts with ESR1; the interaction is enhanced by phosphorylation of ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interacts with DDX17. Interacts with BRDT. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Akap8Q630144EBI-643076,EBI-11617845From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-32293N.
IntActiQ61656. 12 interactors.
MINTiMINT-1549637.
STRINGi10090.ENSMUSP00000021062.

Structurei

3D structure databases

ProteinModelPortaliQ61656.
SMRiQ61656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 300Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini328 – 475Helicase C-terminalPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni477 – 614Transactivation domainBy similarityAdd BLAST138

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi94 – 122Q motifAdd BLAST29
Motifi248 – 251DEAD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
HOVERGENiHBG015893.
InParanoidiQ61656.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR033488. DDX5.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR012587. P68_rpt.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF257. PTHR24031:SF257. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF08061. P68HR. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM01414. P68HR. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSYSSDRDR GRDRGFGAPR FGGSRTGPLS GKKFGNPGEK LVKKKWNLDE
60 70 80 90 100
LPKFEKNFYQ EHPDLARRTA QEVDTYRRSK EITVRGHNCP KPVLNFYEAN
110 120 130 140 150
FPANVMDVIA RQNFTEPTAI QAQGWPVALS GLDMVGVAQT GSGKTLSYLL
160 170 180 190 200
PAIVHINHQP FLERGDGPIC LVLAPTRELA QQVQQVAAEY CRACRLKSTC
210 220 230 240 250
IYGGAPKGPQ IRDLERGVEI CIATPGRLID FLECGKTNLR RTTYLVLDEA
260 270 280 290 300
DRMLDMGFEP QIRKIVDQIR PDRQTLMWSA TWPKEVRQLA EDFLKDYIHI
310 320 330 340 350
NIGALELSAN HNILQIVDVC HDVEKDEKLI RLMEEIMSEK ENKTIVFVET
360 370 380 390 400
KRRCDELTRK MRRDGWPAMG IHGDKSQQER DWVLNEFKHG KAPILIATDV
410 420 430 440 450
ASRGLDVEDV KFVINYDYPN SSEDYIHRIG RTARSTKTGT AYTFFTPNNI
460 470 480 490 500
KQVSDLISVL REANQAINPK LLQLVEDRGS GRSRGRGGMK DDRRDRYSAG
510 520 530 540 550
KRGGFNTFRD RENYDRGYSN LLKRDFGAKT QNGVYSAANY TNGSFGSNFV
560 570 580 590 600
SAGIQTSFRT GNPTGTYQNG YDSTQQYGSN VANMHNGMNQ QAYAYPLPQA
610
APMIGYPMPT GYSQ
Length:614
Mass (Da):69,290
Last modified:July 27, 2011 - v2
Checksum:iA9BBB25D9B7B8819
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112Q → H in CAA46581 (PubMed:8445986).Curated1
Sequence conflicti159Q → H in CAA46581 (PubMed:8445986).Curated1
Sequence conflicti597L → V in CAA46581 (PubMed:8445986).Curated1
Sequence conflicti600A → P in CAA46581 (PubMed:8445986).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65627 mRNA. Translation: CAA46581.1.
AL603664 Genomic DNA. No translation available.
PIRiI48385.
UniGeneiMm.491096.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65627 mRNA. Translation: CAA46581.1.
AL603664 Genomic DNA. No translation available.
PIRiI48385.
UniGeneiMm.491096.

3D structure databases

ProteinModelPortaliQ61656.
SMRiQ61656.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-32293N.
IntActiQ61656. 12 interactors.
MINTiMINT-1549637.
STRINGi10090.ENSMUSP00000021062.

PTM databases

iPTMnetiQ61656.
PhosphoSitePlusiQ61656.
SwissPalmiQ61656.

Proteomic databases

EPDiQ61656.
MaxQBiQ61656.
PaxDbiQ61656.
PRIDEiQ61656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:105037. Ddx5.

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
HOVERGENiHBG015893.
InParanoidiQ61656.

Enzyme and pathway databases

BRENDAi3.6.4.13. 3474.

Miscellaneous databases

ChiTaRSiDdx5. mouse.
PROiQ61656.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DDX5.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR033488. DDX5.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR012587. P68_rpt.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF257. PTHR24031:SF257. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF08061. P68HR. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM01414. P68HR. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX5_MOUSE
AccessioniPrimary (citable) accession number: Q61656
Secondary accession number(s): E9Q105
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Silenced gene expression via RNA interference in PubMed:17011493 and PubMed:17960593 was simultaneously performed with DDX5 and DDX17; siRNA-resistant DDX5 expression was able to rescue the effect in muscle differentiation.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.