Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q61656

- DDX5_MOUSE

UniProt

Q61656 - DDX5_MOUSE

Protein

Probable ATP-dependent RNA helicase DDX5

Gene

Ddx5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.3 Publications

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi114 – 1163ATPPROSITE-ProRule annotation
    Nucleotide bindingi138 – 1458ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. androgen receptor binding Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. ATP-dependent helicase activity Source: InterPro
    4. estrogen receptor binding Source: UniProtKB
    5. protein binding Source: UniProtKB
    6. RNA binding Source: UniProtKB-KW
    7. RNA helicase activity Source: UniProtKB
    8. transcription coactivator activity Source: UniProtKB
    9. transcription cofactor activity Source: MGI

    GO - Biological processi

    1. circadian rhythm Source: MGI
    2. intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
    3. in utero embryonic development Source: MGI
    4. mRNA processing Source: UniProtKB-KW
    5. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    6. positive regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
    7. positive regulation of transcription, DNA-templated Source: MGI
    8. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    9. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
    10. regulation of androgen receptor signaling pathway Source: UniProtKB
    11. regulation of osteoblast differentiation Source: UniProtKB
    12. regulation of skeletal muscle cell differentiation Source: UniProtKB
    13. RNA splicing Source: UniProtKB-KW
    14. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Biological rhythms, mRNA processing, mRNA splicing, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DDX5 (EC:3.6.4.13)
    Alternative name(s):
    DEAD box RNA helicase DEAD1
    Short name:
    mDEAD1
    DEAD box protein 5
    RNA helicase p68
    Gene namesi
    Name:Ddx5
    Synonyms:Tnz2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:105037. Ddx5.

    Subcellular locationi

    Nucleusnucleolus By similarity

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB
    2. nucleus Source: MGI
    3. ribonucleoprotein complex Source: MGI
    4. spliceosomal complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 614614Probable ATP-dependent RNA helicase DDX5PRO_0000054992Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei32 – 321N6-acetyllysineBy similarity
    Modified residuei33 – 331N6-acetyllysineBy similarity
    Modified residuei40 – 401N6-acetyllysine1 Publication
    Cross-linki53 – 53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei236 – 2361N6-acetyllysine1 Publication
    Modified residuei297 – 2971PhosphotyrosineBy similarity
    Modified residuei480 – 4801PhosphoserineBy similarity
    Modified residuei502 – 5021Omega-N-methylated arginineBy similarity

    Post-translational modificationi

    Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination By similarity.By similarity
    Polyubiquitinated, leading to proteasomal degradation.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ61656.
    PaxDbiQ61656.
    PRIDEiQ61656.

    PTM databases

    PhosphoSiteiQ61656.

    Expressioni

    Gene expression databases

    CleanExiMM_DDX5.
    GenevestigatoriQ61656.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex. Interacts with RBM4; the interaction occurs in an RNA-independent manner. Interacts with AGO1 and AGO2. Interacts with ESR1; the interaction is enhanced by phosphorylation of ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interacts with DDX17. Interacts with BRDT. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active).2 Publications

    Protein-protein interaction databases

    DIPiDIP-32293N.
    IntActiQ61656. 9 interactions.
    MINTiMINT-1549637.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61656.
    SMRiQ61656. Positions 50-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini125 – 300176Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini328 – 475148Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni477 – 614138Transactivation domainBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi94 – 12229Q motifAdd
    BLAST
    Motifi248 – 2514DEAD box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    HOVERGENiHBG015893.
    InParanoidiQ61656.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR012587. P68HR.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF08061. P68HR. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61656-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSYSSDRDR GRDRGFGAPR FGGSRTGPLS GKKFGNPGEK LVKKKWNLDE    50
    LPKFEKNFYQ EHPDLARRTA QEVDTYRRSK EITVRGHNCP KPVLNFYEAN 100
    FPANVMDVIA RQNFTEPTAI QAQGWPVALS GLDMVGVAQT GSGKTLSYLL 150
    PAIVHINHQP FLERGDGPIC LVLAPTRELA QQVQQVAAEY CRACRLKSTC 200
    IYGGAPKGPQ IRDLERGVEI CIATPGRLID FLECGKTNLR RTTYLVLDEA 250
    DRMLDMGFEP QIRKIVDQIR PDRQTLMWSA TWPKEVRQLA EDFLKDYIHI 300
    NIGALELSAN HNILQIVDVC HDVEKDEKLI RLMEEIMSEK ENKTIVFVET 350
    KRRCDELTRK MRRDGWPAMG IHGDKSQQER DWVLNEFKHG KAPILIATDV 400
    ASRGLDVEDV KFVINYDYPN SSEDYIHRIG RTARSTKTGT AYTFFTPNNI 450
    KQVSDLISVL REANQAINPK LLQLVEDRGS GRSRGRGGMK DDRRDRYSAG 500
    KRGGFNTFRD RENYDRGYSN LLKRDFGAKT QNGVYSAANY TNGSFGSNFV 550
    SAGIQTSFRT GNPTGTYQNG YDSTQQYGSN VANMHNGMNQ QAYAYPLPQA 600
    APMIGYPMPT GYSQ 614
    Length:614
    Mass (Da):69,290
    Last modified:July 27, 2011 - v2
    Checksum:iA9BBB25D9B7B8819
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti112 – 1121Q → H in CAA46581. (PubMed:8445986)Curated
    Sequence conflicti159 – 1591Q → H in CAA46581. (PubMed:8445986)Curated
    Sequence conflicti597 – 5971L → V in CAA46581. (PubMed:8445986)Curated
    Sequence conflicti600 – 6001A → P in CAA46581. (PubMed:8445986)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65627 mRNA. Translation: CAA46581.1.
    AL603664 Genomic DNA. No translation available.
    PIRiI48385.
    UniGeneiMm.491096.

    Genome annotation databases

    UCSCiuc007lzs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65627 mRNA. Translation: CAA46581.1 .
    AL603664 Genomic DNA. No translation available.
    PIRi I48385.
    UniGenei Mm.491096.

    3D structure databases

    ProteinModelPortali Q61656.
    SMRi Q61656. Positions 50-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-32293N.
    IntActi Q61656. 9 interactions.
    MINTi MINT-1549637.

    PTM databases

    PhosphoSitei Q61656.

    Proteomic databases

    MaxQBi Q61656.
    PaxDbi Q61656.
    PRIDEi Q61656.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi uc007lzs.1. mouse.

    Organism-specific databases

    MGIi MGI:105037. Ddx5.

    Phylogenomic databases

    eggNOGi COG0513.
    HOVERGENi HBG015893.
    InParanoidi Q61656.

    Miscellaneous databases

    ChiTaRSi DDX5. mouse.
    PROi Q61656.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_DDX5.
    Genevestigatori Q61656.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR012587. P68HR.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF08061. P68HR. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "High-level expression in male germ cells of murine P68 RNA helicase mRNA."
      Lemaire L., Heinlein U.A.O.
      Life Sci. 52:917-926(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation."
      Caretti G., Schiltz R.L., Dilworth F.J., Di Padova M., Zhao P., Ogryzko V., Fuller-Pace F.V., Hoffman E.P., Tapscott S.J., Sartorelli V.
      Dev. Cell 11:547-560(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: FUNCTION.
    5. "The testis-specific double bromodomain-containing protein BRDT forms a complex with multiple spliceosome components and is required for mRNA splicing and 3'-UTR truncation in round spermatids."
      Berkovits B.D., Wang L., Guarnieri P., Wolgemuth D.J.
      Nucleic Acids Res. 40:7162-7175(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BRDT.
    6. "Feedback regulation of transcriptional termination by the mammalian circadian clock PERIOD complex."
      Padmanabhan K., Robles M.S., Westerling T., Weitz C.J.
      Science 337:599-602(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CIRCADIAN RHYTHMS, IDENTIFICATION IN A LARGE PER COMPLEX, SUBCELLULAR LOCATION.
    7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40 AND LYS-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiDDX5_MOUSE
    AccessioniPrimary (citable) accession number: Q61656
    Secondary accession number(s): E9Q105
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Silenced gene expression via RNA interference in PubMed:17011493 and PubMed:17960593 was simultaneously performed with DDX5 and DDX17; siRNA-resistant DDX5 expression was able to rescue the effect in muscle differentiation.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3