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Q61656

- DDX5_MOUSE

UniProt

Q61656 - DDX5_MOUSE

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Protein

Probable ATP-dependent RNA helicase DDX5

Gene

Ddx5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi114 – 1163ATPPROSITE-ProRule annotation
Nucleotide bindingi138 – 1458ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. androgen receptor binding Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. ATP-dependent helicase activity Source: InterPro
  4. estrogen receptor binding Source: UniProtKB
  5. RNA binding Source: UniProtKB-KW
  6. RNA helicase activity Source: UniProtKB
  7. transcription coactivator activity Source: UniProtKB
  8. transcription cofactor activity Source: MGI

GO - Biological processi

  1. circadian rhythm Source: MGI
  2. intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
  3. in utero embryonic development Source: MGI
  4. mRNA processing Source: UniProtKB-KW
  5. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  6. positive regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  7. positive regulation of transcription, DNA-templated Source: MGI
  8. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  9. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  10. regulation of androgen receptor signaling pathway Source: UniProtKB
  11. regulation of osteoblast differentiation Source: UniProtKB
  12. regulation of skeletal muscle cell differentiation Source: UniProtKB
  13. RNA splicing Source: UniProtKB-KW
  14. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Biological rhythms, mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX5 (EC:3.6.4.13)
Alternative name(s):
DEAD box RNA helicase DEAD1
Short name:
mDEAD1
DEAD box protein 5
RNA helicase p68
Gene namesi
Name:Ddx5
Synonyms:Tnz2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:105037. Ddx5.

Subcellular locationi

Nucleusnucleolus By similarity

GO - Cellular componenti

  1. nucleolus Source: UniProtKB
  2. nucleus Source: MGI
  3. ribonucleoprotein complex Source: MGI
  4. spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Probable ATP-dependent RNA helicase DDX5PRO_0000054992Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321N6-acetyllysineBy similarity
Modified residuei33 – 331N6-acetyllysineBy similarity
Modified residuei40 – 401N6-acetyllysine1 Publication
Cross-linki53 – 53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei236 – 2361N6-acetyllysine1 Publication
Modified residuei297 – 2971PhosphotyrosineBy similarity
Modified residuei480 – 4801PhosphoserineBy similarity
Modified residuei502 – 5021Omega-N-methylated arginineBy similarity

Post-translational modificationi

Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination (By similarity).By similarity
Polyubiquitinated, leading to proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ61656.
PaxDbiQ61656.
PRIDEiQ61656.

PTM databases

PhosphoSiteiQ61656.

Expressioni

Gene expression databases

CleanExiMM_DDX5.
GenevestigatoriQ61656.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with RBM4; the interaction occurs in an RNA-independent manner. Interacts with AGO1 and AGO2. Interacts with ESR1; the interaction is enhanced by phosphorylation of ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interacts with DDX17. Interacts with BRDT. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active).2 Publications

Protein-protein interaction databases

DIPiDIP-32293N.
IntActiQ61656. 9 interactions.
MINTiMINT-1549637.

Structurei

3D structure databases

ProteinModelPortaliQ61656.
SMRiQ61656. Positions 50-473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 300176Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini328 – 475148Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni477 – 614138Transactivation domainBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi94 – 12229Q motifAdd
BLAST
Motifi248 – 2514DEAD box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0513.
HOVERGENiHBG015893.
InParanoidiQ61656.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR012587. P68HR.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF08061. P68HR. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61656-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSYSSDRDR GRDRGFGAPR FGGSRTGPLS GKKFGNPGEK LVKKKWNLDE
60 70 80 90 100
LPKFEKNFYQ EHPDLARRTA QEVDTYRRSK EITVRGHNCP KPVLNFYEAN
110 120 130 140 150
FPANVMDVIA RQNFTEPTAI QAQGWPVALS GLDMVGVAQT GSGKTLSYLL
160 170 180 190 200
PAIVHINHQP FLERGDGPIC LVLAPTRELA QQVQQVAAEY CRACRLKSTC
210 220 230 240 250
IYGGAPKGPQ IRDLERGVEI CIATPGRLID FLECGKTNLR RTTYLVLDEA
260 270 280 290 300
DRMLDMGFEP QIRKIVDQIR PDRQTLMWSA TWPKEVRQLA EDFLKDYIHI
310 320 330 340 350
NIGALELSAN HNILQIVDVC HDVEKDEKLI RLMEEIMSEK ENKTIVFVET
360 370 380 390 400
KRRCDELTRK MRRDGWPAMG IHGDKSQQER DWVLNEFKHG KAPILIATDV
410 420 430 440 450
ASRGLDVEDV KFVINYDYPN SSEDYIHRIG RTARSTKTGT AYTFFTPNNI
460 470 480 490 500
KQVSDLISVL REANQAINPK LLQLVEDRGS GRSRGRGGMK DDRRDRYSAG
510 520 530 540 550
KRGGFNTFRD RENYDRGYSN LLKRDFGAKT QNGVYSAANY TNGSFGSNFV
560 570 580 590 600
SAGIQTSFRT GNPTGTYQNG YDSTQQYGSN VANMHNGMNQ QAYAYPLPQA
610
APMIGYPMPT GYSQ
Length:614
Mass (Da):69,290
Last modified:July 27, 2011 - v2
Checksum:iA9BBB25D9B7B8819
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121Q → H in CAA46581. (PubMed:8445986)Curated
Sequence conflicti159 – 1591Q → H in CAA46581. (PubMed:8445986)Curated
Sequence conflicti597 – 5971L → V in CAA46581. (PubMed:8445986)Curated
Sequence conflicti600 – 6001A → P in CAA46581. (PubMed:8445986)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65627 mRNA. Translation: CAA46581.1.
AL603664 Genomic DNA. No translation available.
PIRiI48385.
UniGeneiMm.491096.

Genome annotation databases

UCSCiuc007lzs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65627 mRNA. Translation: CAA46581.1 .
AL603664 Genomic DNA. No translation available.
PIRi I48385.
UniGenei Mm.491096.

3D structure databases

ProteinModelPortali Q61656.
SMRi Q61656. Positions 50-473.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-32293N.
IntActi Q61656. 9 interactions.
MINTi MINT-1549637.

PTM databases

PhosphoSitei Q61656.

Proteomic databases

MaxQBi Q61656.
PaxDbi Q61656.
PRIDEi Q61656.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi uc007lzs.1. mouse.

Organism-specific databases

MGIi MGI:105037. Ddx5.

Phylogenomic databases

eggNOGi COG0513.
HOVERGENi HBG015893.
InParanoidi Q61656.

Miscellaneous databases

ChiTaRSi Ddx5. mouse.
PROi Q61656.
SOURCEi Search...

Gene expression databases

CleanExi MM_DDX5.
Genevestigatori Q61656.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR012587. P68HR.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF08061. P68HR. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "High-level expression in male germ cells of murine P68 RNA helicase mRNA."
    Lemaire L., Heinlein U.A.O.
    Life Sci. 52:917-926(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation."
    Caretti G., Schiltz R.L., Dilworth F.J., Di Padova M., Zhao P., Ogryzko V., Fuller-Pace F.V., Hoffman E.P., Tapscott S.J., Sartorelli V.
    Dev. Cell 11:547-560(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: FUNCTION.
  5. "The testis-specific double bromodomain-containing protein BRDT forms a complex with multiple spliceosome components and is required for mRNA splicing and 3'-UTR truncation in round spermatids."
    Berkovits B.D., Wang L., Guarnieri P., Wolgemuth D.J.
    Nucleic Acids Res. 40:7162-7175(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BRDT.
  6. "Feedback regulation of transcriptional termination by the mammalian circadian clock PERIOD complex."
    Padmanabhan K., Robles M.S., Westerling T., Weitz C.J.
    Science 337:599-602(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CIRCADIAN RHYTHMS, IDENTIFICATION IN A LARGE PER COMPLEX, SUBCELLULAR LOCATION.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40 AND LYS-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiDDX5_MOUSE
AccessioniPrimary (citable) accession number: Q61656
Secondary accession number(s): E9Q105
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Silenced gene expression via RNA interference in PubMed:17011493 and PubMed:17960593 was simultaneously performed with DDX5 and DDX17; siRNA-resistant DDX5 expression was able to rescue the effect in muscle differentiation.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3