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Q61655

- DD19A_MOUSE

UniProt

Q61655 - DD19A_MOUSE

Protein

ATP-dependent RNA helicase DDX19A

Gene

Ddx19a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19 functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181ATPBy similarity
    Binding sitei428 – 4281ATPBy similarity
    Binding sitei431 – 4311ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi137 – 1448ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA transport Source: UniProtKB-KW
    2. positive regulation of apoptotic process Source: Ensembl
    3. protein transport Source: UniProtKB-KW
    4. response to zinc ion Source: Ensembl

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    mRNA transport, Protein transport, Translocation, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent RNA helicase DDX19A (EC:3.6.4.13)
    Alternative name(s):
    DEAD box RNA helicase DEAD5
    Short name:
    mDEAD5
    DEAD box protein 19A
    Eukaryotic translation initiation factor 4A-related sequence 1
    Gene namesi
    Name:Ddx19a
    Synonyms:Ddx19, Eif4a-rs1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:99526. Ddx19a.

    Subcellular locationi

    Cytoplasm By similarity. Nucleusnuclear pore complex. Nucleus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Nuclear pore complex cytoplasmic fibrils.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear membrane Source: UniProtKB-SubCell
    3. nuclear pore Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nuclear pore complex, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 478477ATP-dependent RNA helicase DDX19APRO_0000055023Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ61655.
    PaxDbiQ61655.
    PRIDEiQ61655.

    2D gel databases

    REPRODUCTION-2DPAGEQ61655.

    PTM databases

    PhosphoSiteiQ61655.

    Expressioni

    Tissue specificityi

    Found in testis, heart, brain, liver, skeletal muscle, and kidney.

    Gene expression databases

    BgeeiQ61655.
    CleanExiMM_DDX19A.
    GenevestigatoriQ61655.

    Interactioni

    Protein-protein interaction databases

    BioGridi199417. 2 interactions.
    IntActiQ61655. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61655.
    SMRiQ61655. Positions 59-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini124 – 294171Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini305 – 473169Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 299298N-terminal lobeBy similarityAdd
    BLAST
    Regioni54 – 6714N-terminal helixBy similarityAdd
    BLAST
    Regioni300 – 478179C-terminal lobeBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi91 – 11929Q motifAdd
    BLAST
    Motifi241 – 2444DEAD box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi48 – 536Poly-Glu

    Domaini

    The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.By similarity

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    GeneTreeiENSGT00530000063236.
    HOGENOMiHOG000268797.
    HOVERGENiHBG107989.
    InParanoidiQ543M2.
    OMAiMMAINTI.
    OrthoDBiEOG7TMZRJ.
    TreeFamiTF314957.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61655-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATDSWALAV DEQEAAVKSM SSLQIKEEKV KADTNGVIKT STTAEKTEEE    50
    EKEDRAAQSL LNKLIRSNLV DNTNQVEVLQ RDPSSPLYSV KSFEELRLKP 100
    QLLQGVYAMG FNRPSKIQEN ALPMMLAEPP QNLIAQSQSG TGKTAAFVLA 150
    MLSRVEPADR YPQCLCLSPT YELALQTGKV IEQMGKFHPE LKLAYAVRGN 200
    KLERGQKVSE QIVIGTPGTV LDWCSKLKFI DPKKIKVFVL DEADVMIATQ 250
    GHQDQSIRIQ RMLPRNCQML LFSATFEDSV WKFAQKVVPD PNIIKLKREE 300
    ETLDTIKQYY VLCNNREEKF QALCNLYGAI TIAQAMIFCH TRKTASWLAA 350
    ELSKEGHQVA LLSGEMMVEQ RAAVIERFRE GKEKVLVTTN VCARGIDVEQ 400
    VSVVINFDLP VDKDGNPDNE TYLHRIGRTG RFGKRGLAVN MVDSKHSMNI 450
    LNRIQEHFNK KIERLDTDDL DEIEKIAN 478
    Length:478
    Mass (Da):53,933
    Last modified:July 27, 2011 - v2
    Checksum:i46BDE331544EDA7E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 323VKA → AKS in AAA53629. (PubMed:8144024)Curated
    Sequence conflicti262 – 2632ML → IV in AAA53629. (PubMed:8144024)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25125 mRNA. Translation: AAA53629.1.
    AK049467 mRNA. Translation: BAC33762.1.
    AK146548 mRNA. Translation: BAE27252.1.
    CH466525 Genomic DNA. Translation: EDL11480.1.
    BC011270 mRNA. Translation: AAH11270.1.
    CCDSiCCDS22668.1.
    PIRiI49731.
    RefSeqiNP_031942.2. NM_007916.2.
    UniGeneiMm.287901.

    Genome annotation databases

    EnsembliENSMUST00000040416; ENSMUSP00000047898; ENSMUSG00000015023.
    GeneIDi13680.
    KEGGimmu:13680.
    UCSCiuc009nll.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25125 mRNA. Translation: AAA53629.1 .
    AK049467 mRNA. Translation: BAC33762.1 .
    AK146548 mRNA. Translation: BAE27252.1 .
    CH466525 Genomic DNA. Translation: EDL11480.1 .
    BC011270 mRNA. Translation: AAH11270.1 .
    CCDSi CCDS22668.1.
    PIRi I49731.
    RefSeqi NP_031942.2. NM_007916.2.
    UniGenei Mm.287901.

    3D structure databases

    ProteinModelPortali Q61655.
    SMRi Q61655. Positions 59-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199417. 2 interactions.
    IntActi Q61655. 1 interaction.

    PTM databases

    PhosphoSitei Q61655.

    2D gel databases

    REPRODUCTION-2DPAGE Q61655.

    Proteomic databases

    MaxQBi Q61655.
    PaxDbi Q61655.
    PRIDEi Q61655.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040416 ; ENSMUSP00000047898 ; ENSMUSG00000015023 .
    GeneIDi 13680.
    KEGGi mmu:13680.
    UCSCi uc009nll.2. mouse.

    Organism-specific databases

    CTDi 55308.
    MGIi MGI:99526. Ddx19a.

    Phylogenomic databases

    eggNOGi COG0513.
    GeneTreei ENSGT00530000063236.
    HOGENOMi HOG000268797.
    HOVERGENi HBG107989.
    InParanoidi Q543M2.
    OMAi MMAINTI.
    OrthoDBi EOG7TMZRJ.
    TreeFami TF314957.

    Miscellaneous databases

    ChiTaRSi DDX19A. mouse.
    NextBioi 284434.
    PROi Q61655.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61655.
    CleanExi MM_DDX19A.
    Genevestigatori Q61655.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse erythroid cells express multiple putative RNA helicase genes exhibiting high sequence conservation from yeast to mammals."
      Gee S.L., Conboy J.G.
      Gene 140:171-177(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Erythroleukemia.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo and Stomach.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiDD19A_MOUSE
    AccessioniPrimary (citable) accession number: Q61655
    Secondary accession number(s): Q543M2, Q921R0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3