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Protein

ATP-dependent RNA helicase DDX19A

Gene

Ddx19a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19 functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118ATPBy similarity1
Binding sitei428ATPBy similarity1
Binding sitei431ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 144ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX19A (EC:3.6.4.13)
Alternative name(s):
DEAD box RNA helicase DEAD5
Short name:
mDEAD5
DEAD box protein 19A
Eukaryotic translation initiation factor 4A-related sequence 1
Gene namesi
Name:Ddx19a
Synonyms:Ddx19, Eif4a-rs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:99526. Ddx19a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000550232 – 478ATP-dependent RNA helicase DDX19AAdd BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki26Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki26Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ61655.
MaxQBiQ61655.
PaxDbiQ61655.
PeptideAtlasiQ61655.
PRIDEiQ61655.

2D gel databases

REPRODUCTION-2DPAGEQ61655.

PTM databases

iPTMnetiQ61655.
PhosphoSitePlusiQ61655.

Expressioni

Tissue specificityi

Found in testis, heart, brain, liver, skeletal muscle, and kidney.

Gene expression databases

BgeeiENSMUSG00000015023.
CleanExiMM_DDX19A.
GenevisibleiQ61655. MM.

Interactioni

Protein-protein interaction databases

BioGridi199417. 2 interactors.
IntActiQ61655. 1 interactor.
STRINGi10090.ENSMUSP00000047898.

Structurei

3D structure databases

ProteinModelPortaliQ61655.
SMRiQ61655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 294Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini305 – 473Helicase C-terminalPROSITE-ProRule annotationAdd BLAST169

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 299N-terminal lobeBy similarityAdd BLAST298
Regioni54 – 67N-terminal helixBy similarityAdd BLAST14
Regioni300 – 478C-terminal lobeBy similarityAdd BLAST179

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi91 – 119Q motifAdd BLAST29
Motifi241 – 244DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi48 – 53Poly-Glu6

Domaini

The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.By similarity

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ61655.
KOiK18655.
OMAiQAICISP.
OrthoDBiEOG091G03PS.
TreeFamiTF314957.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61655-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDSWALAV DEQEAAVKSM SSLQIKEEKV KADTNGVIKT STTAEKTEEE
60 70 80 90 100
EKEDRAAQSL LNKLIRSNLV DNTNQVEVLQ RDPSSPLYSV KSFEELRLKP
110 120 130 140 150
QLLQGVYAMG FNRPSKIQEN ALPMMLAEPP QNLIAQSQSG TGKTAAFVLA
160 170 180 190 200
MLSRVEPADR YPQCLCLSPT YELALQTGKV IEQMGKFHPE LKLAYAVRGN
210 220 230 240 250
KLERGQKVSE QIVIGTPGTV LDWCSKLKFI DPKKIKVFVL DEADVMIATQ
260 270 280 290 300
GHQDQSIRIQ RMLPRNCQML LFSATFEDSV WKFAQKVVPD PNIIKLKREE
310 320 330 340 350
ETLDTIKQYY VLCNNREEKF QALCNLYGAI TIAQAMIFCH TRKTASWLAA
360 370 380 390 400
ELSKEGHQVA LLSGEMMVEQ RAAVIERFRE GKEKVLVTTN VCARGIDVEQ
410 420 430 440 450
VSVVINFDLP VDKDGNPDNE TYLHRIGRTG RFGKRGLAVN MVDSKHSMNI
460 470
LNRIQEHFNK KIERLDTDDL DEIEKIAN
Length:478
Mass (Da):53,933
Last modified:July 27, 2011 - v2
Checksum:i46BDE331544EDA7E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30 – 32VKA → AKS in AAA53629 (PubMed:8144024).Curated3
Sequence conflicti262 – 263ML → IV in AAA53629 (PubMed:8144024).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25125 mRNA. Translation: AAA53629.1.
AK049467 mRNA. Translation: BAC33762.1.
AK146548 mRNA. Translation: BAE27252.1.
CH466525 Genomic DNA. Translation: EDL11480.1.
BC011270 mRNA. Translation: AAH11270.1.
CCDSiCCDS22668.1.
PIRiI49731.
RefSeqiNP_031942.2. NM_007916.2.
UniGeneiMm.287901.

Genome annotation databases

EnsembliENSMUST00000040416; ENSMUSP00000047898; ENSMUSG00000015023.
GeneIDi13680.
KEGGimmu:13680.
UCSCiuc009nll.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25125 mRNA. Translation: AAA53629.1.
AK049467 mRNA. Translation: BAC33762.1.
AK146548 mRNA. Translation: BAE27252.1.
CH466525 Genomic DNA. Translation: EDL11480.1.
BC011270 mRNA. Translation: AAH11270.1.
CCDSiCCDS22668.1.
PIRiI49731.
RefSeqiNP_031942.2. NM_007916.2.
UniGeneiMm.287901.

3D structure databases

ProteinModelPortaliQ61655.
SMRiQ61655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199417. 2 interactors.
IntActiQ61655. 1 interactor.
STRINGi10090.ENSMUSP00000047898.

PTM databases

iPTMnetiQ61655.
PhosphoSitePlusiQ61655.

2D gel databases

REPRODUCTION-2DPAGEQ61655.

Proteomic databases

EPDiQ61655.
MaxQBiQ61655.
PaxDbiQ61655.
PeptideAtlasiQ61655.
PRIDEiQ61655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040416; ENSMUSP00000047898; ENSMUSG00000015023.
GeneIDi13680.
KEGGimmu:13680.
UCSCiuc009nll.2. mouse.

Organism-specific databases

CTDi55308.
MGIiMGI:99526. Ddx19a.

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ61655.
KOiK18655.
OMAiQAICISP.
OrthoDBiEOG091G03PS.
TreeFamiTF314957.

Miscellaneous databases

ChiTaRSiDdx19a. mouse.
PROiQ61655.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015023.
CleanExiMM_DDX19A.
GenevisibleiQ61655. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDD19A_MOUSE
AccessioniPrimary (citable) accession number: Q61655
Secondary accession number(s): Q543M2, Q921R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.