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Protein

Hyaluronan synthase 1

Gene

Has1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.1 Publication

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).1 Publication
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).1 Publication

Cofactori

Kineticsi

  1. KM=0.8 mM for UDP-Glc-NAc (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication
  2. KM=0.7 mM for UDP-Glc-UA (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication

    Pathwayi: hyaluronan biosynthesis

    This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.

    GO - Molecular functioni

    • hyaluronan synthase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.212. 3474.
    ReactomeiR-MMU-2142850. Hyaluronan biosynthesis and export.
    UniPathwayiUPA00341.

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronan synthase 1 (EC:2.4.1.212)
    Alternative name(s):
    Hyaluronate synthase 1
    Hyaluronic acid synthase 1
    Short name:
    HA synthase 1
    Gene namesi
    Name:Has1
    Synonyms:Has
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 17

    Organism-specific databases

    MGIiMGI:106590. Has1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
    Transmembranei25 – 45Helical; Name=1Sequence analysisAdd BLAST21
    Topological domaini46 – 51ExtracellularSequence analysis6
    Transmembranei52 – 72Helical; Name=2Sequence analysisAdd BLAST21
    Topological domaini73 – 404CytoplasmicSequence analysisAdd BLAST332
    Transmembranei405 – 425Helical; Name=3Sequence analysisAdd BLAST21
    Topological domaini426 – 435ExtracellularSequence analysis10
    Transmembranei436 – 456Helical; Name=4Sequence analysisAdd BLAST21
    Topological domaini457 – 462CytoplasmicSequence analysis6
    Transmembranei463 – 483Helical; Name=5Sequence analysisAdd BLAST21
    Topological domaini484 – 501ExtracellularSequence analysisAdd BLAST18
    Transmembranei502 – 522Helical; Name=6Sequence analysisAdd BLAST21
    Topological domaini523 – 545CytoplasmicSequence analysisAdd BLAST23
    Transmembranei546 – 566Helical; Name=7Sequence analysisAdd BLAST21
    Topological domaini567 – 583ExtracellularSequence analysisAdd BLAST17

    GO - Cellular componenti

    • cytoplasm Source: CACAO
    • integral component of plasma membrane Source: GO_Central
    • membrane Source: MGI
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi242D → E: Loss of both activities. 1 Publication1
    Mutagenesisi311S → N: No effect. 1 Publication1
    Mutagenesisi312G → P: No effect. 1 Publication1
    Mutagenesisi313P → G: No effect. 1 Publication1
    Mutagenesisi314L → I: 75% decrease of both activities. 1 Publication1
    Mutagenesisi314L → V: Loss of HA activity. 1 Publication1
    Mutagenesisi344D → E: Loss of both activities. 1 Publication1
    Mutagenesisi380Q → N: 85%-90% decrease of both activities. 1 Publication1
    Mutagenesisi383R → K: 85%-90% decrease of both activities. 1 Publication1
    Mutagenesisi384W → Y: Loss of both activities. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001971701 – 583Hyaluronan synthase 1Add BLAST583

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi489N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ61647.
    PRIDEiQ61647.

    PTM databases

    iPTMnetiQ61647.
    PhosphoSitePlusiQ61647.

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000003665.
    CleanExiMM_HAS1.
    GenevisibleiQ61647. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000003762.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61647.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi81 – 84Poly-Ala4
    Compositional biasi516 – 519Poly-Leu4

    Sequence similaritiesi

    Belongs to the NodC/HAS family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2571. Eukaryota.
    COG1215. LUCA.
    GeneTreeiENSGT00390000010337.
    HOGENOMiHOG000112847.
    HOVERGENiHBG000189.
    InParanoidiQ61647.
    KOiK00752.
    OMAiRTHRCVC.
    OrthoDBiEOG091G04P3.
    PhylomeDBiQ61647.
    TreeFamiTF332506.

    Family and domain databases

    Gene3Di3.90.550.10. 2 hits.
    InterProiIPR026107. HAS/NodC.
    IPR028385. HAS1.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF4. PTHR22913:SF4. 1 hit.
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q61647-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRQDMPKPSE AARCCSGLAR RALTIIFALL ILGLMTWAYA AGVPLASDRY
    60 70 80 90 100
    GLLAFGLYGA FLSAHLVAQS LFAYLEHRRV AAAARRSLAK GPLDAATARS
    110 120 130 140 150
    VALTISAYQE DPAYLRQCLT SARALLYPHT RLRVLMVVDG NRAEDLYMVD
    160 170 180 190 200
    MFREVFADED PATYVWDGNY HQPWEPAEAT GAVGEGAYRE VEAEDPGRLA
    210 220 230 240 250
    VEALVRTRRC VCVAQRWGGK REVMYTAFKA LGDSVDYVQV CDSDTRLDPM
    260 270 280 290 300
    ALLELVRVLD EDPRVGAVGG DVRILNPLDS WVSFLSSLRY WVAFNVERAC
    310 320 330 340 350
    QSYFHCVSCI SGPLGLYRNN LLQQFLEAWY NQKFLGTHCT FGDDRHLTNR
    360 370 380 390 400
    MLSMGYATKY TSRSRCYSET PSSFLRWLSQ QTRWSKSYFR EWLYNALWWH
    410 420 430 440 450
    RHHAWMTYEA VVSGLFPFFV AATVLRLFYA GRPWALLWVL LCVQGVALAK
    460 470 480 490 500
    AAFAAWLRGC VRMVLLSLYA PLYMCGLLPA KFLALVTMNQ SGWGTSGRKK
    510 520 530 540 550
    LAANYVPVLP LALWALLLLG GLARSVAQEA RADWSGPSRA AEAYHLAAGA
    560 570 580
    GAYVAYWVVM LTIYWVGVRR LCRRRSGGYR VQV
    Length:583
    Mass (Da):65,545
    Last modified:November 1, 1996 - v1
    Checksum:i7AF9273E7B314728
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D82964 mRNA. Translation: BAA11654.1.
    CCDSiCCDS37459.1.
    RefSeqiNP_032241.1. NM_008215.2.
    UniGeneiMm.255701.

    Genome annotation databases

    EnsembliENSMUST00000003762; ENSMUSP00000003762; ENSMUSG00000003665.
    GeneIDi15116.
    KEGGimmu:15116.
    UCSCiuc008apr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D82964 mRNA. Translation: BAA11654.1.
    CCDSiCCDS37459.1.
    RefSeqiNP_032241.1. NM_008215.2.
    UniGeneiMm.255701.

    3D structure databases

    ProteinModelPortaliQ61647.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000003762.

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.

    PTM databases

    iPTMnetiQ61647.
    PhosphoSitePlusiQ61647.

    Proteomic databases

    PaxDbiQ61647.
    PRIDEiQ61647.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000003762; ENSMUSP00000003762; ENSMUSG00000003665.
    GeneIDi15116.
    KEGGimmu:15116.
    UCSCiuc008apr.2. mouse.

    Organism-specific databases

    CTDi3036.
    MGIiMGI:106590. Has1.

    Phylogenomic databases

    eggNOGiKOG2571. Eukaryota.
    COG1215. LUCA.
    GeneTreeiENSGT00390000010337.
    HOGENOMiHOG000112847.
    HOVERGENiHBG000189.
    InParanoidiQ61647.
    KOiK00752.
    OMAiRTHRCVC.
    OrthoDBiEOG091G04P3.
    PhylomeDBiQ61647.
    TreeFamiTF332506.

    Enzyme and pathway databases

    UniPathwayiUPA00341.
    BRENDAi2.4.1.212. 3474.
    ReactomeiR-MMU-2142850. Hyaluronan biosynthesis and export.

    Miscellaneous databases

    PROiQ61647.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000003665.
    CleanExiMM_HAS1.
    GenevisibleiQ61647. MM.

    Family and domain databases

    Gene3Di3.90.550.10. 2 hits.
    InterProiIPR026107. HAS/NodC.
    IPR028385. HAS1.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF4. PTHR22913:SF4. 1 hit.
    SUPFAMiSSF53448. SSF53448. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHYAS1_MOUSE
    AccessioniPrimary (citable) accession number: Q61647
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.