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Q61647

- HYAS1_MOUSE

UniProt

Q61647 - HYAS1_MOUSE

Protein

Hyaluronan synthase 1

Gene

Has1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.1 Publication

    Catalytic activityi

    UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).1 Publication
    UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).1 Publication

    Cofactori

    Magnesium.

    Kineticsi

    1. KM=0.8 mM for UDP-Glc-NAc (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication
    2. KM=0.7 mM for UDP-Glc-UA (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication

    Pathwayi

    GO - Molecular functioni

    1. hyaluronan synthase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to platelet-derived growth factor stimulus Source: Ensembl
    2. extracellular matrix assembly Source: UniProtKB
    3. extracellular polysaccharide biosynthetic process Source: UniProtKB
    4. hyaluronan biosynthetic process Source: MGI
    5. negative regulation of fibroblast migration Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.212. 3474.
    ReactomeiREACT_196552. Hyaluronan biosynthesis and export.
    UniPathwayiUPA00341.

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronan synthase 1 (EC:2.4.1.212)
    Alternative name(s):
    Hyaluronate synthase 1
    Hyaluronic acid synthase 1
    Short name:
    HA synthase 1
    Gene namesi
    Name:Has1
    Synonyms:Has
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:106590. Has1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi242 – 2421D → E: Loss of both activities. 1 Publication
    Mutagenesisi311 – 3111S → N: No effect. 1 Publication
    Mutagenesisi312 – 3121G → P: No effect. 1 Publication
    Mutagenesisi313 – 3131P → G: No effect. 1 Publication
    Mutagenesisi314 – 3141L → I: 75% decrease of both activities. 1 Publication
    Mutagenesisi314 – 3141L → V: Loss of HA activity. 1 Publication
    Mutagenesisi344 – 3441D → E: Loss of both activities. 1 Publication
    Mutagenesisi380 – 3801Q → N: 85%-90% decrease of both activities. 1 Publication
    Mutagenesisi383 – 3831R → K: 85%-90% decrease of both activities. 1 Publication
    Mutagenesisi384 – 3841W → Y: Loss of both activities. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 583583Hyaluronan synthase 1PRO_0000197170Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ61647.

    PTM databases

    PhosphoSiteiQ61647.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61647.
    BgeeiQ61647.
    CleanExiMM_HAS1.
    GenevestigatoriQ61647.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61647.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini46 – 516ExtracellularSequence Analysis
    Topological domaini73 – 404332CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini426 – 43510ExtracellularSequence Analysis
    Topological domaini457 – 4626CytoplasmicSequence Analysis
    Topological domaini484 – 50118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini523 – 54523CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini567 – 58317ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4521Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei52 – 7221Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei405 – 42521Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei436 – 45621Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei463 – 48321Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei502 – 52221Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei546 – 56621Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi81 – 844Poly-Ala
    Compositional biasi516 – 5194Poly-Leu

    Sequence similaritiesi

    Belongs to the NodC/HAS family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1215.
    GeneTreeiENSGT00390000010337.
    HOGENOMiHOG000112847.
    HOVERGENiHBG000189.
    InParanoidiQ61647.
    KOiK00752.
    OMAiHRCVCVA.
    OrthoDBiEOG77WWBZ.
    PhylomeDBiQ61647.
    TreeFamiTF332506.

    Family and domain databases

    Gene3Di3.90.550.10. 2 hits.
    InterProiIPR026107. HAS/NodC.
    IPR028385. HAS1.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF4. PTHR22913:SF4. 1 hit.
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q61647-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQDMPKPSE AARCCSGLAR RALTIIFALL ILGLMTWAYA AGVPLASDRY    50
    GLLAFGLYGA FLSAHLVAQS LFAYLEHRRV AAAARRSLAK GPLDAATARS 100
    VALTISAYQE DPAYLRQCLT SARALLYPHT RLRVLMVVDG NRAEDLYMVD 150
    MFREVFADED PATYVWDGNY HQPWEPAEAT GAVGEGAYRE VEAEDPGRLA 200
    VEALVRTRRC VCVAQRWGGK REVMYTAFKA LGDSVDYVQV CDSDTRLDPM 250
    ALLELVRVLD EDPRVGAVGG DVRILNPLDS WVSFLSSLRY WVAFNVERAC 300
    QSYFHCVSCI SGPLGLYRNN LLQQFLEAWY NQKFLGTHCT FGDDRHLTNR 350
    MLSMGYATKY TSRSRCYSET PSSFLRWLSQ QTRWSKSYFR EWLYNALWWH 400
    RHHAWMTYEA VVSGLFPFFV AATVLRLFYA GRPWALLWVL LCVQGVALAK 450
    AAFAAWLRGC VRMVLLSLYA PLYMCGLLPA KFLALVTMNQ SGWGTSGRKK 500
    LAANYVPVLP LALWALLLLG GLARSVAQEA RADWSGPSRA AEAYHLAAGA 550
    GAYVAYWVVM LTIYWVGVRR LCRRRSGGYR VQV 583
    Length:583
    Mass (Da):65,545
    Last modified:November 1, 1996 - v1
    Checksum:i7AF9273E7B314728
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D82964 mRNA. Translation: BAA11654.1.
    CCDSiCCDS37459.1.
    RefSeqiNP_032241.1. NM_008215.2.
    UniGeneiMm.255701.

    Genome annotation databases

    EnsembliENSMUST00000003762; ENSMUSP00000003762; ENSMUSG00000003665.
    GeneIDi15116.
    KEGGimmu:15116.
    UCSCiuc008apr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D82964 mRNA. Translation: BAA11654.1 .
    CCDSi CCDS37459.1.
    RefSeqi NP_032241.1. NM_008215.2.
    UniGenei Mm.255701.

    3D structure databases

    ProteinModelPortali Q61647.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT2. Glycosyltransferase Family 2.

    PTM databases

    PhosphoSitei Q61647.

    Proteomic databases

    PRIDEi Q61647.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003762 ; ENSMUSP00000003762 ; ENSMUSG00000003665 .
    GeneIDi 15116.
    KEGGi mmu:15116.
    UCSCi uc008apr.2. mouse.

    Organism-specific databases

    CTDi 3036.
    MGIi MGI:106590. Has1.

    Phylogenomic databases

    eggNOGi COG1215.
    GeneTreei ENSGT00390000010337.
    HOGENOMi HOG000112847.
    HOVERGENi HBG000189.
    InParanoidi Q61647.
    KOi K00752.
    OMAi HRCVCVA.
    OrthoDBi EOG77WWBZ.
    PhylomeDBi Q61647.
    TreeFami TF332506.

    Enzyme and pathway databases

    UniPathwayi UPA00341 .
    BRENDAi 2.4.1.212. 3474.
    Reactomei REACT_196552. Hyaluronan biosynthesis and export.

    Miscellaneous databases

    NextBioi 287530.
    PROi Q61647.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61647.
    Bgeei Q61647.
    CleanExi MM_HAS1.
    Genevestigatori Q61647.

    Family and domain databases

    Gene3Di 3.90.550.10. 2 hits.
    InterProi IPR026107. HAS/NodC.
    IPR028385. HAS1.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF4. PTHR22913:SF4. 1 hit.
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase."
      Itano N., Kimata K.
      J. Biol. Chem. 271:9875-9878(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 199-206, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    3. Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, KINETIC PARAMETERS.
    4. "In vitro synthesis of hyaluronan by a single protein derived from mouse HAS1 gene and characterization of amino acid residues essential for the activity."
      Yoshida M., Itano N., Yamada Y., Kimata K.
      J. Biol. Chem. 275:497-506(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.

    Entry informationi

    Entry nameiHYAS1_MOUSE
    AccessioniPrimary (citable) accession number: Q61647
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3