Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q61647 (HYAS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronan synthase 1

EC=2.4.1.212
Alternative name(s):
Hyaluronate synthase 1
Hyaluronic acid synthase 1
Short name=HA synthase 1
Gene names
Name:Has1
Synonyms:Has
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate. Ref.3

Catalytic activity

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). Ref.3

UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan). Ref.3

Cofactor

Magnesium.

Pathway

Glycan biosynthesis; hyaluronan biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the NodC/HAS family.

Biophysicochemical properties

Kinetic parameters:

KM=0.8 mM for UDP-Glc-NAc (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2) Ref.3

KM=0.7 mM for UDP-Glc-UA (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 583583Hyaluronan synthase 1
PRO_0000197170

Regions

Topological domain1 – 2424Cytoplasmic Potential
Transmembrane25 – 4521Helical; Name=1; Potential
Topological domain46 – 516Extracellular Potential
Transmembrane52 – 7221Helical; Name=2; Potential
Topological domain73 – 404332Cytoplasmic Potential
Transmembrane405 – 42521Helical; Name=3; Potential
Topological domain426 – 43510Extracellular Potential
Transmembrane436 – 45621Helical; Name=4; Potential
Topological domain457 – 4626Cytoplasmic Potential
Transmembrane463 – 48321Helical; Name=5; Potential
Topological domain484 – 50118Extracellular Potential
Transmembrane502 – 52221Helical; Name=6; Potential
Topological domain523 – 54523Cytoplasmic Potential
Transmembrane546 – 56621Helical; Name=7; Potential
Topological domain567 – 58317Extracellular Potential
Compositional bias81 – 844Poly-Ala
Compositional bias516 – 5194Poly-Leu

Amino acid modifications

Glycosylation4891N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis2421D → E: Loss of both activities.
Mutagenesis3111S → N: No effect.
Mutagenesis3121G → P: No effect.
Mutagenesis3131P → G: No effect.
Mutagenesis3141L → I: 75% decrease of both activities.
Mutagenesis3141L → V: Loss of HA activity.
Mutagenesis3441D → E: Loss of both activities.
Mutagenesis3801Q → N: 85%-90% decrease of both activities.
Mutagenesis3831R → K: 85%-90% decrease of both activities.
Mutagenesis3841W → Y: Loss of both activities.

Sequences

Sequence LengthMass (Da)Tools
Q61647 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7AF9273E7B314728

FASTA58365,545
        10         20         30         40         50         60 
MRQDMPKPSE AARCCSGLAR RALTIIFALL ILGLMTWAYA AGVPLASDRY GLLAFGLYGA 

        70         80         90        100        110        120 
FLSAHLVAQS LFAYLEHRRV AAAARRSLAK GPLDAATARS VALTISAYQE DPAYLRQCLT 

       130        140        150        160        170        180 
SARALLYPHT RLRVLMVVDG NRAEDLYMVD MFREVFADED PATYVWDGNY HQPWEPAEAT 

       190        200        210        220        230        240 
GAVGEGAYRE VEAEDPGRLA VEALVRTRRC VCVAQRWGGK REVMYTAFKA LGDSVDYVQV 

       250        260        270        280        290        300 
CDSDTRLDPM ALLELVRVLD EDPRVGAVGG DVRILNPLDS WVSFLSSLRY WVAFNVERAC 

       310        320        330        340        350        360 
QSYFHCVSCI SGPLGLYRNN LLQQFLEAWY NQKFLGTHCT FGDDRHLTNR MLSMGYATKY 

       370        380        390        400        410        420 
TSRSRCYSET PSSFLRWLSQ QTRWSKSYFR EWLYNALWWH RHHAWMTYEA VVSGLFPFFV 

       430        440        450        460        470        480 
AATVLRLFYA GRPWALLWVL LCVQGVALAK AAFAAWLRGC VRMVLLSLYA PLYMCGLLPA 

       490        500        510        520        530        540 
KFLALVTMNQ SGWGTSGRKK LAANYVPVLP LALWALLLLG GLARSVAQEA RADWSGPSRA 

       550        560        570        580 
AEAYHLAAGA GAYVAYWVVM LTIYWVGVRR LCRRRSGGYR VQV 

« Hide

References

[1]"Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase."
Itano N., Kimata K.
J. Biol. Chem. 271:9875-9878(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 199-206, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[3]"Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties."
Itano N., Sawai T., Yoshida M., Lenas P., Yamada Y., Imagawa M., Shinomura T., Hamaguchi M., Yoshida Y., Ohnuki Y., Miyauchi S., Spicer A.P., McDonald J.A., Kimata K.
J. Biol. Chem. 274:25085-25092(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, KINETIC PARAMETERS.
[4]"In vitro synthesis of hyaluronan by a single protein derived from mouse HAS1 gene and characterization of amino acid residues essential for the activity."
Yoshida M., Itano N., Yamada Y., Kimata K.
J. Biol. Chem. 275:497-506(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D82964 mRNA. Translation: BAA11654.1.
CCDSCCDS37459.1.
RefSeqNP_032241.1. NM_008215.2.
UniGeneMm.255701.

3D structure databases

ProteinModelPortalQ61647.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT2. Glycosyltransferase Family 2.

PTM databases

PhosphoSiteQ61647.

Proteomic databases

PRIDEQ61647.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003762; ENSMUSP00000003762; ENSMUSG00000003665.
GeneID15116.
KEGGmmu:15116.
UCSCuc008apr.2. mouse.

Organism-specific databases

CTD3036.
MGIMGI:106590. Has1.

Phylogenomic databases

eggNOGCOG1215.
GeneTreeENSGT00390000010337.
HOGENOMHOG000112847.
HOVERGENHBG000189.
InParanoidQ61647.
KOK00752.
OMAHRCVCVA.
OrthoDBEOG77WWBZ.
PhylomeDBQ61647.
TreeFamTF332506.

Enzyme and pathway databases

BRENDA2.4.1.212. 3474.
UniPathwayUPA00341.

Gene expression databases

ArrayExpressQ61647.
BgeeQ61647.
CleanExMM_HAS1.
GenevestigatorQ61647.

Family and domain databases

Gene3D3.90.550.10. 2 hits.
InterProIPR026107. HAS/NodC.
IPR028385. HAS1.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF4. PTHR22913:SF4. 1 hit.
SUPFAMSSF53448. SSF53448. 2 hits.
ProtoNetSearch...

Other

NextBio287530.
PROQ61647.
SOURCESearch...

Entry information

Entry nameHYAS1_MOUSE
AccessionPrimary (citable) accession number: Q61647
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot