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Protein

Hyaluronan synthase 1

Gene

Has1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.1 Publication

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).1 Publication
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).1 Publication

Cofactori

Kineticsi

  1. KM=0.8 mM for UDP-Glc-NAc (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication
  2. KM=0.7 mM for UDP-Glc-UA (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication

Pathwayi

GO - Molecular functioni

  1. hyaluronan synthase activity Source: UniProtKB

GO - Biological processi

  1. cell adhesion Source: GO_Central
  2. cellular response to platelet-derived growth factor stimulus Source: MGI
  3. extracellular matrix assembly Source: UniProtKB
  4. extracellular polysaccharide biosynthetic process Source: UniProtKB
  5. hyaluronan biosynthetic process Source: MGI
  6. negative regulation of fibroblast migration Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.212. 3474.
ReactomeiREACT_299251. Hyaluronan biosynthesis and export.
UniPathwayiUPA00341.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronan synthase 1 (EC:2.4.1.212)
Alternative name(s):
Hyaluronate synthase 1
Hyaluronic acid synthase 1
Short name:
HA synthase 1
Gene namesi
Name:Has1
Synonyms:Has
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:106590. Has1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei25 – 4521Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini46 – 516ExtracellularSequence Analysis
Transmembranei52 – 7221Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini73 – 404332CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei405 – 42521Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini426 – 43510ExtracellularSequence Analysis
Transmembranei436 – 45621Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini457 – 4626CytoplasmicSequence Analysis
Transmembranei463 – 48321Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini484 – 50118ExtracellularSequence AnalysisAdd
BLAST
Transmembranei502 – 52221Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini523 – 54523CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei546 – 56621Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini567 – 58317ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: GO_Central
  2. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi242 – 2421D → E: Loss of both activities. 1 Publication
Mutagenesisi311 – 3111S → N: No effect. 1 Publication
Mutagenesisi312 – 3121G → P: No effect. 1 Publication
Mutagenesisi313 – 3131P → G: No effect. 1 Publication
Mutagenesisi314 – 3141L → I: 75% decrease of both activities. 1 Publication
Mutagenesisi314 – 3141L → V: Loss of HA activity. 1 Publication
Mutagenesisi344 – 3441D → E: Loss of both activities. 1 Publication
Mutagenesisi380 – 3801Q → N: 85%-90% decrease of both activities. 1 Publication
Mutagenesisi383 – 3831R → K: 85%-90% decrease of both activities. 1 Publication
Mutagenesisi384 – 3841W → Y: Loss of both activities. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 583583Hyaluronan synthase 1PRO_0000197170Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ61647.

PTM databases

PhosphoSiteiQ61647.

Expressioni

Gene expression databases

BgeeiQ61647.
CleanExiMM_HAS1.
ExpressionAtlasiQ61647. baseline and differential.
GenevestigatoriQ61647.

Structurei

3D structure databases

ProteinModelPortaliQ61647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 844Poly-Ala
Compositional biasi516 – 5194Poly-Leu

Sequence similaritiesi

Belongs to the NodC/HAS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000010337.
HOGENOMiHOG000112847.
HOVERGENiHBG000189.
InParanoidiQ61647.
KOiK00752.
OMAiRTHRCVC.
OrthoDBiEOG77WWBZ.
PhylomeDBiQ61647.
TreeFamiTF332506.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR026107. HAS/NodC.
IPR028385. HAS1.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF4. PTHR22913:SF4. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q61647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQDMPKPSE AARCCSGLAR RALTIIFALL ILGLMTWAYA AGVPLASDRY
60 70 80 90 100
GLLAFGLYGA FLSAHLVAQS LFAYLEHRRV AAAARRSLAK GPLDAATARS
110 120 130 140 150
VALTISAYQE DPAYLRQCLT SARALLYPHT RLRVLMVVDG NRAEDLYMVD
160 170 180 190 200
MFREVFADED PATYVWDGNY HQPWEPAEAT GAVGEGAYRE VEAEDPGRLA
210 220 230 240 250
VEALVRTRRC VCVAQRWGGK REVMYTAFKA LGDSVDYVQV CDSDTRLDPM
260 270 280 290 300
ALLELVRVLD EDPRVGAVGG DVRILNPLDS WVSFLSSLRY WVAFNVERAC
310 320 330 340 350
QSYFHCVSCI SGPLGLYRNN LLQQFLEAWY NQKFLGTHCT FGDDRHLTNR
360 370 380 390 400
MLSMGYATKY TSRSRCYSET PSSFLRWLSQ QTRWSKSYFR EWLYNALWWH
410 420 430 440 450
RHHAWMTYEA VVSGLFPFFV AATVLRLFYA GRPWALLWVL LCVQGVALAK
460 470 480 490 500
AAFAAWLRGC VRMVLLSLYA PLYMCGLLPA KFLALVTMNQ SGWGTSGRKK
510 520 530 540 550
LAANYVPVLP LALWALLLLG GLARSVAQEA RADWSGPSRA AEAYHLAAGA
560 570 580
GAYVAYWVVM LTIYWVGVRR LCRRRSGGYR VQV
Length:583
Mass (Da):65,545
Last modified:November 1, 1996 - v1
Checksum:i7AF9273E7B314728
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82964 mRNA. Translation: BAA11654.1.
CCDSiCCDS37459.1.
RefSeqiNP_032241.1. NM_008215.2.
UniGeneiMm.255701.

Genome annotation databases

EnsembliENSMUST00000003762; ENSMUSP00000003762; ENSMUSG00000003665.
GeneIDi15116.
KEGGimmu:15116.
UCSCiuc008apr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82964 mRNA. Translation: BAA11654.1.
CCDSiCCDS37459.1.
RefSeqiNP_032241.1. NM_008215.2.
UniGeneiMm.255701.

3D structure databases

ProteinModelPortaliQ61647.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

PTM databases

PhosphoSiteiQ61647.

Proteomic databases

PRIDEiQ61647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003762; ENSMUSP00000003762; ENSMUSG00000003665.
GeneIDi15116.
KEGGimmu:15116.
UCSCiuc008apr.2. mouse.

Organism-specific databases

CTDi3036.
MGIiMGI:106590. Has1.

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000010337.
HOGENOMiHOG000112847.
HOVERGENiHBG000189.
InParanoidiQ61647.
KOiK00752.
OMAiRTHRCVC.
OrthoDBiEOG77WWBZ.
PhylomeDBiQ61647.
TreeFamiTF332506.

Enzyme and pathway databases

UniPathwayiUPA00341.
BRENDAi2.4.1.212. 3474.
ReactomeiREACT_299251. Hyaluronan biosynthesis and export.

Miscellaneous databases

NextBioi287530.
PROiQ61647.
SOURCEiSearch...

Gene expression databases

BgeeiQ61647.
CleanExiMM_HAS1.
ExpressionAtlasiQ61647. baseline and differential.
GenevestigatoriQ61647.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR026107. HAS/NodC.
IPR028385. HAS1.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF4. PTHR22913:SF4. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase."
    Itano N., Kimata K.
    J. Biol. Chem. 271:9875-9878(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 199-206, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  3. Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, KINETIC PARAMETERS.
  4. "In vitro synthesis of hyaluronan by a single protein derived from mouse HAS1 gene and characterization of amino acid residues essential for the activity."
    Yoshida M., Itano N., Yamada Y., Kimata K.
    J. Biol. Chem. 275:497-506(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiHYAS1_MOUSE
AccessioniPrimary (citable) accession number: Q61647
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.