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Q61646 (HPT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Haptoglobin
Alternative name(s):
Zonulin

Cleaved into the following 2 chains:

  1. Haptoglobin alpha chain
  2. Haptoglobin beta chain
Gene names
Name:Hp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway By similarity.

Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens By similarity.

Subunit structure

Tetramer of two alpha and two beta chains; disufide-linked. The Hemoglobin/haptoglobin complex is composed of a haptoglobin dimer bound to two hemoglobin alpha-beta dimers. Interacts with CD163 By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed by the liver and secreted in plasma.

Domain

The beta chain mediates most of the interactions with both subunits of hemoglobin, while the alpha chain forms the homodimeric interface By similarity.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Contains 1 Sushi (CCP/SCR) domain.

Caution

Although homologous to serine proteases, it has lost all essential catalytic residues and has no enzymatic activity.

Ontologies

Keywords
   Biological processAcute phase
Immunity
   Cellular componentSecreted
   DomainSignal
Sushi
   LigandHemoglobin-binding
   Molecular functionAntibiotic
Antimicrobial
Antioxidant
Serine protease homolog
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacute-phase response

Inferred from electronic annotation. Source: UniProtKB-KW

defense response to bacterium

Inferred from electronic annotation. Source: UniProtKB-KW

immune system process

Inferred from electronic annotation. Source: UniProtKB-KW

liver development

Inferred from electronic annotation. Source: Ensembl

organ regeneration

Inferred from electronic annotation. Source: Ensembl

response to L-ascorbic acid

Inferred from electronic annotation. Source: Ensembl

response to X-ray

Inferred from electronic annotation. Source: Ensembl

response to cobalamin

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

response to heat

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to lead ion

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to magnesium ion

Inferred from electronic annotation. Source: Ensembl

spermatogenesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionantioxidant activity

Inferred from electronic annotation. Source: UniProtKB-KW

catalytic activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 347329Haptoglobin
PRO_0000028462
Chain19 – 10183Haptoglobin alpha chain
PRO_0000028463
Chain103 – 347245Haptoglobin beta chain
PRO_0000028464

Regions

Domain31 – 8858Sushi
Domain103 – 345243Peptidase S1
Region259 – 2646Interaction with CD163 By similarity

Amino acid modifications

Glycosylation1481N-linked (GlcNAc...) Ref.2
Glycosylation1821N-linked (GlcNAc...) Ref.2
Glycosylation2561N-linked (GlcNAc...) Ref.2
Glycosylation2641N-linked (GlcNAc...) Potential
Disulfide bond33Interchain By similarity
Disulfide bond52 ↔ 86 By similarity
Disulfide bond90 ↔ 207Interchain (between alpha and beta chains) By similarity
Disulfide bond250 ↔ 281 By similarity
Disulfide bond292 ↔ 322 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q61646 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 63822D121232F130

FASTA34738,752
        10         20         30         40         50         60 
MRALGAVVTL LLWGQLFAVE LGNDAMDFED DSCPKPPEIA NGYVEHLVRY RCRQFYRLRA 

        70         80         90        100        110        120 
EGDGVYTLND EKQWVNTVAG EKLPECEAVC GKPKHPVDQV QRIIGGSMDA KGSFPWQAKM 

       130        140        150        160        170        180 
ISRHGLTTGA TLISDQWLLT TAKNLFLNHS ETASAKDITP TLTLYVGKNQ LVEIEKVVLH 

       190        200        210        220        230        240 
PNHSVVDIGL IKLKQRVLVT ERVMPICLPS KDYIAPGRVG YVSGWGRNAN FRFTDRLKYV 

       250        260        270        280        290        300 
MLPVADQDKC VVHYENSTVP EKKNLTSPVG VQPILNEHTF CAGLTKYQED TCYGDAGSAF 

       310        320        330        340 
AIHDMEEDTW YAAGILSFDK SCAVAEYGVY VRATDLKDWV QETMAKN 

« Hide

References

« Hide 'large scale' references
[1]"Expression of haptoglobin in mouse adipose tissue."
Ponte P.A., White R.T., Uyeda C.M., Coleman R.T.
Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
[2]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-148; ASN-182 AND ASN-256.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96827 mRNA. Translation: AAA37779.1.
CCDSCCDS40470.1.
RefSeqNP_059066.1. NM_017370.2.
UniGeneMm.26730.

3D structure databases

ProteinModelPortalQ61646.
SMRQ61646. Positions 33-345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61646. 2 interactions.
MINTMINT-1870167.

Protein family/group databases

MEROPSS01.972.

PTM databases

PhosphoSiteQ61646.

Proteomic databases

MaxQBQ61646.
PaxDbQ61646.
PRIDEQ61646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000074898; ENSMUSP00000074436; ENSMUSG00000031722.
GeneID15439.
KEGGmmu:15439.
UCSCuc009nin.2. mouse.

Organism-specific databases

CTD3240.
MGIMGI:96211. Hp.

Phylogenomic databases

eggNOGNOG246387.
GeneTreeENSGT00560000076882.
HOGENOMHOG000112945.
HOVERGENHBG005989.
InParanoidQ61646.
KOK16142.
OMADDTWYAA.
OrthoDBEOG722J8R.
PhylomeDBQ61646.
TreeFamTF334326.

Gene expression databases

ArrayExpressQ61646.
BgeeQ61646.
CleanExMM_HP.
GenevestigatorQ61646.

Family and domain databases

InterProIPR008292. Haptoglobin.
IPR001254. Peptidase_S1.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFPIRSF001137. Haptoglobin. 1 hit.
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00032. CCP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEPS50923. SUSHI. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHP. mouse.
NextBio288222.
PROQ61646.
SOURCESearch...

Entry information

Entry nameHPT_MOUSE
AccessionPrimary (citable) accession number: Q61646
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot