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Protein

Haptoglobin

Gene

Hp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).By similarity
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Antioxidant, Serine protease homolog

Keywords - Biological processi

Acute phase, Immunity

Keywords - Ligandi

Hemoglobin-binding

Enzyme and pathway databases

ReactomeiR-MMU-2168880. Scavenging of heme from plasma.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiS01.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Haptoglobin
Alternative name(s):
Zonulin
Cleaved into the following 2 chains:
Gene namesi
Name:Hp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:96211. Hp.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002846219 – 347HaptoglobinAdd BLAST329
ChainiPRO_000002846319 – 101Haptoglobin alpha chainAdd BLAST83
ChainiPRO_0000028464103 – 347Haptoglobin beta chainAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33InterchainBy similarity
Disulfide bondi52 ↔ 86By similarity
Disulfide bondi90 ↔ 207Interchain (between alpha and beta chains)PROSITE-ProRule annotation
Glycosylationi148N-linked (GlcNAc...)1 Publication1
Glycosylationi182N-linked (GlcNAc...)1 Publication1
Disulfide bondi250 ↔ 281By similarity
Glycosylationi256N-linked (GlcNAc...)1 Publication1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi292 ↔ 322By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61646.
PaxDbiQ61646.
PeptideAtlasiQ61646.
PRIDEiQ61646.

PTM databases

iPTMnetiQ61646.
PhosphoSitePlusiQ61646.
SwissPalmiQ61646.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiENSMUSG00000031722.
CleanExiMM_HP.
ExpressionAtlasiQ61646. baseline and differential.
GenevisibleiQ61646. MM.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains; disufide-linked. The Hemoglobin/haptoglobin complex is composed of a haptoglobin dimer bound to two hemoglobin alpha-beta dimers. Interacts with CD163 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ61646. 2 interactors.
MINTiMINT-1870167.
STRINGi10090.ENSMUSP00000074436.

Structurei

3D structure databases

ProteinModelPortaliQ61646.
SMRiQ61646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 88SushiPROSITE-ProRule annotationAdd BLAST58
Domaini103 – 345Peptidase S1PROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni259 – 264Interaction with CD163By similarity6

Domaini

The beta chain mediates most of the interactions with both subunits of hemoglobin, while the alpha chain forms the homodimeric interface.By similarity

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 Sushi (CCP/SCR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118890.
HOGENOMiHOG000112945.
HOVERGENiHBG005989.
InParanoidiQ61646.
KOiK16142.
OMAiWQAKMIS.
OrthoDBiEOG091G099M.
PhylomeDBiQ61646.
TreeFamiTF334326.

Family and domain databases

CDDicd00033. CCP. 1 hit.
cd00190. Tryp_SPc. 1 hit.
InterProiIPR008292. Haptoglobin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP_dom.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001137. Haptoglobin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEiPS50923. SUSHI. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALGAVVTL LLWGQLFAVE LGNDAMDFED DSCPKPPEIA NGYVEHLVRY
60 70 80 90 100
RCRQFYRLRA EGDGVYTLND EKQWVNTVAG EKLPECEAVC GKPKHPVDQV
110 120 130 140 150
QRIIGGSMDA KGSFPWQAKM ISRHGLTTGA TLISDQWLLT TAKNLFLNHS
160 170 180 190 200
ETASAKDITP TLTLYVGKNQ LVEIEKVVLH PNHSVVDIGL IKLKQRVLVT
210 220 230 240 250
ERVMPICLPS KDYIAPGRVG YVSGWGRNAN FRFTDRLKYV MLPVADQDKC
260 270 280 290 300
VVHYENSTVP EKKNLTSPVG VQPILNEHTF CAGLTKYQED TCYGDAGSAF
310 320 330 340
AIHDMEEDTW YAAGILSFDK SCAVAEYGVY VRATDLKDWV QETMAKN
Length:347
Mass (Da):38,752
Last modified:November 1, 1996 - v1
Checksum:i63822D121232F130
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96827 mRNA. Translation: AAA37779.1.
CCDSiCCDS40470.1.
RefSeqiNP_059066.1. NM_017370.2.
UniGeneiMm.26730.

Genome annotation databases

EnsembliENSMUST00000074898; ENSMUSP00000074436; ENSMUSG00000031722.
GeneIDi15439.
KEGGimmu:15439.
UCSCiuc009nin.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96827 mRNA. Translation: AAA37779.1.
CCDSiCCDS40470.1.
RefSeqiNP_059066.1. NM_017370.2.
UniGeneiMm.26730.

3D structure databases

ProteinModelPortaliQ61646.
SMRiQ61646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61646. 2 interactors.
MINTiMINT-1870167.
STRINGi10090.ENSMUSP00000074436.

Protein family/group databases

MEROPSiS01.972.

PTM databases

iPTMnetiQ61646.
PhosphoSitePlusiQ61646.
SwissPalmiQ61646.

Proteomic databases

MaxQBiQ61646.
PaxDbiQ61646.
PeptideAtlasiQ61646.
PRIDEiQ61646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074898; ENSMUSP00000074436; ENSMUSG00000031722.
GeneIDi15439.
KEGGimmu:15439.
UCSCiuc009nin.2. mouse.

Organism-specific databases

CTDi3240.
MGIiMGI:96211. Hp.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118890.
HOGENOMiHOG000112945.
HOVERGENiHBG005989.
InParanoidiQ61646.
KOiK16142.
OMAiWQAKMIS.
OrthoDBiEOG091G099M.
PhylomeDBiQ61646.
TreeFamiTF334326.

Enzyme and pathway databases

ReactomeiR-MMU-2168880. Scavenging of heme from plasma.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiHp. mouse.
PROiQ61646.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031722.
CleanExiMM_HP.
ExpressionAtlasiQ61646. baseline and differential.
GenevisibleiQ61646. MM.

Family and domain databases

CDDicd00033. CCP. 1 hit.
cd00190. Tryp_SPc. 1 hit.
InterProiIPR008292. Haptoglobin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP_dom.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001137. Haptoglobin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEiPS50923. SUSHI. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHPT_MOUSE
AccessioniPrimary (citable) accession number: Q61646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although homologous to serine proteases, it has lost all essential catalytic residues and has no enzymatic activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.