Q61625 (GRID2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate receptor ionotropic, delta-2
Short name=GluD2
Short name=GluR delta-2 subunit

Gene names

Name:

Grid2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1007 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
Subunit structure	Interacts with AIP1 and AP4M1 By similarity. Interacts with BECN1, GOPC, GRID2IP, SHANK1 and SHANK2. Interacts with CBLN1 and CBLN2, but not with CBLN4. CBLN1-binding is calcium-independent. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed selectively in cerebellar Purkinje cells where it is localized in dendritic spines. Ref.1 Ref.4
Domain	The PDZ-binding motif mediates interaction with GOPC. Ref.3
Involvement in disease	Defects in Grid2 are the cause of the Lurcher phenotype. Heterozygous animals display a characteristic swaying of the hind quarters and jerky up and down movements following cerebellar Purkinje cell degeneration during postnatal development. Homozygous animals die shortly after birth because of a massive loss of midbrain and hindbrain neurons during late embryogenesis.
Sequence similarities	Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Disease	Disease mutation
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein localization Inferred from direct assay Ref.4. Source: BHF-UCL ionotropic glutamate receptor signaling pathway Inferred from electronic annotation. Source: GOC prepulse inhibition Inferred from mutant phenotype PubMed 11488959. Source: MGI regulation of excitatory postsynaptic membrane potential Inferred from mutant phenotype PubMed 7766857. Source: MGI regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 10884318. Source: MGI regulation of neuron projection development Inferred from mutant phenotype PubMed 7891151. Source: MGI synaptic transmission, glutamatergic Inferred from mutant phenotype PubMed 7766857. Source: MGI
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW dendritic spine Inferred from direct assay Ref.4. Source: BHF-UCL ionotropic glutamate receptor complex Inferred from direct assay Ref.4. Source: BHF-UCL outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro postsynaptic membrane Inferred from direct assay Ref.4. Source: BHF-UCL
Molecular_function	extracellular-glutamate-gated ion channel activity Inferred from electronic annotation. Source: InterPro ionotropic glutamate receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cbln1	Q9R171	8	EBI-2794106,EBI-2794140

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 1007	984	Glutamate receptor ionotropic, delta-2		PRO_0000011565
Regions
	Topological domain	24 – 566	543	Extracellular Potential
	Transmembrane	567 – 587	21	Helical; Potential
	Topological domain	588 – 635	48	Cytoplasmic Potential
	Transmembrane	636 – 656	21	Helical; Potential
	Topological domain	657 – 830	174	Extracellular Potential
	Transmembrane	831 – 851	21	Helical; Potential
	Topological domain	852 – 1007	156	Cytoplasmic Potential
	Region	921 – 991	71	Interaction with AP4M1 By similarity
	Motif	1005 – 1007	3	PDZ-binding
Amino acid modifications
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	654	1	A → T in Lurcher. Ref.9
Experimental info
	Mutagenesis	920	1	S → A: Abolishes interaction with SHANK1 and SHANK2. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q61625 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A456166CC782A44B	FASTA	1,007	113,082
	10 20 30 40 50 60 MEVFPLLLFL SFCWSRTWDL ATADSIIHIG AIFDESAKKD DEVFRTAVGD LNQNEEILQT 70 80 90 100 110 120 EKITFSVTFV DGNNPFQAVQ EACELMNQGI LALVSSIGCT SAGSLQSLAD AMHIPHLFIQ 130 140 150 160 170 180 RSTAGTPRSG CGLTRSNRND DYTLSVRPPV YLNEVILRVV TEYAWQKFII FYDSEYDIRG 190 200 210 220 230 240 IQEFLDKVSQ QGMDVALQKV ENNINKMITT LFDTMRIEEL NRYRDTLRRA ILVMNPATAK 250 260 270 280 290 300 SFISEVVETN LVAFDCHWII INEEINDVDV QELVRRSIGR LTIIRQTFPV PQNISQRCFR 310 320 330 340 350 360 GNHRISSSLC DPKDPFAQNM EISNLYIYDT VLLLANAFHK KLEDRKWHSM ASLSCIRKNS 370 380 390 400 410 420 KPWQGGRSML ETIKKGGVNG LTGDLEFGEN GGNPNVHFEI LGTNYGEELG RGVRKLGCWN 430 440 450 460 470 480 PVTGLNGSLT DKKLENNMRG VVLRVVTVLE EPFVMVSENV LGKPKKYQGF SIDVLDALSN 490 500 510 520 530 540 YLGFNYEIYV APDHKYGSPQ EDGTWNGLVG ELVFKRADIG ISALTITPDR ENVVDFTTRY 550 560 570 580 590 600 MDYSVGVLLR RAEKTVDMFA CLAPFDLSLW ACIAGTVLLV GLLVYLLNWL NPPRLQMGSM 610 620 630 640 650 660 TSTTLYNSMW FVYGSFVQQG GEVPYTTLAT RMMMGAWWLF ALIVISSYTA NLAAFLTITR 670 680 690 700 710 720 IESSIQSLQD LSKQTDIPYG TVLDSAVYQH VRMKGLNPFE RDSMYSQMWR MINRSNGSEN 730 740 750 760 770 780 NVLESQAGIQ KVKYGNYAFV WDAAVLEYVA INDPDCSFYT VGNTVADRGY GIALQHGSPY 790 800 810 820 830 840 RDVFSQRILE LQQSGDMDIL KHKWWPKNGQ CDLYSSVDAK QKGGALDIKS LAGVFCILAA 850 860 870 880 890 900 GIVLSCLIAV LETWWSRRKG SRVPSKEDDK EIDLEHLHRR VNSLCTDDDS PHKQFSTSSI 910 920 930 940 950 960 DLTPLDIDTL PTRQALEQIS DFRNTHITTT TFIPEQIQTL SRTLSAKAAS GFAFGSVPEH 970 980 990 1000 RTGPFRHRAP NGGFFRSPIK TMSSIPYQPT PTLGLNLGND PDRGTSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Selective expression of the glutamate receptor channel delta 2 subunit in cerebellar Purkinje cells." Araki K., Meguro H., Kushiya E., Takayama C., Inoue Y., Mishina M. Biochem. Biophys. Res. Commun. 197:1267-1276(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: ICR. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice." Yue Z., Horton A., Bravin M., DeJager P.L., Selimi F., Heintz N. Neuron 35:921-933(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GOPC AND BECN1, DOMAIN.
[4]	"Direct interaction of GluRdelta2 with Shank scaffold proteins in cerebellar Purkinje cells." Uemura T., Mori H., Mishina M. Mol. Cell. Neurosci. 26:330-341(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SHANK1 AND SHANK2, MUTAGENESIS OF SER-920, TISSUE SPECIFICITY.
[5]	"Binding of glutamate receptor delta2 to its scaffold protein, Delphilin, is regulated by PKA." Sonoda T., Mochizuki C., Yamashita T., Watanabe-Kaneko K., Miyagi Y., Shigeri Y., Yazama F., Okuda K., Kawamoto S. Biochem. Biophys. Res. Commun. 350:748-752(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GRID2IP.
[6]	"Cbln1 is a ligand for an orphan glutamate receptor delta2, a bidirectional synapse organizer." Matsuda K., Miura E., Miyazaki T., Kakegawa W., Emi K., Narumi S., Fukazawa Y., Ito-Ishida A., Kondo T., Shigemoto R., Watanabe M., Yuzaki M. Science 328:363-368(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CBLN1.
[7]	"Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions." Matsuda K., Yuzaki M. Eur. J. Neurosci. 33:1447-1461(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CBLN1.
[8]	"The Cbln family of proteins interact with multiple signaling pathways." Wei P., Pattarini R., Rong Y., Guo H., Bansal P.K., Kusnoor S.V., Deutch A.Y., Parris J., Morgan J.I. J. Neurochem. 121:717-729(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CBLN1 AND CBLN2.
[9]	"Neurodegeneration in Lurcher mice caused by mutation in delta2 glutamate receptor gene." Zuo J., De Jager P.L., Takahashi K.A., Jiang W., Linden D.J., Heintz N. Nature 388:769-773(1997) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LURCHER THR-654. Tissue: Purkinje cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13266 mRNA. Translation: BAA02524.1. BC139823 mRNA. Translation: AAI39824.1.
IPI	IPI00123534.
PIR	PN0156.
RefSeq	NP_032193.1. NM_008167.2.
UniGene	Mm.439651. Mm.447390.
3D structure databases
ProteinModelPortal	Q61625.
SMR	Q61625. Positions 26-426, 441-610, 658-806.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61625. 35 interactions.
MINT	MINT-126960.
STRING	10090.ENSMUSP00000093536.
Protein family/group databases
TCDB	1.A.10.1.8. glutamate-gated ion channel (GIC) family of neurotransmitter receptors.
PTM databases
PhosphoSite	Q61625.
Proteomic databases
PaxDb	Q61625.
PRIDE	Q61625.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000095852; ENSMUSP00000093536; ENSMUSG00000071424.
GeneID	14804.
KEGG	mmu:14804.
UCSC	uc009cdz.1. mouse.
Organism-specific databases
CTD	2895.
MGI	MGI:95813. Grid2.
Phylogenomic databases
eggNOG	NOG276334.
GeneTree	ENSGT00700000104113.
HOGENOM	HOG000264260.
HOVERGEN	HBG051840.
InParanoid	A4QPG1.
KO	K05207.
OMA	CSFYTVG.
OrthoDB	EOG4SBDX4.
Gene expression databases
ArrayExpress	Q61625.
Bgee	Q61625.
CleanEx	MM_GRID2.
Genevestigator	Q61625.
GermOnline	ENSMUSG00000071424. Mus musculus.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR019594. Glu_rcpt_Glu/Gly-bd. IPR001320. Iontro_glu_rcpt. IPR001508. NMDA_rcpt. IPR001638. SBP_bac_3. [Graphical view]
Pfam	PF01094. ANF_receptor. 1 hit. PF00060. Lig_chan. 1 hit. PF00497. SBP_bac_3. 1 hit. [Graphical view]
PRINTS	PR00177. NMDARECEPTOR.
SMART	SM00918. Lig_chan-Glu_bd. 1 hit. SM00079. PBPe. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q61625.
ChiTaRS	GRID2. mouse.
NextBio	286973.
SOURCE	Search...

Entry information

Entry name

GRID2_MOUSE

Accession

Primary (citable) accession number: Q61625
Secondary accession number(s): A4QPG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program