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Q61625

- GRID2_MOUSE

UniProt

Q61625 - GRID2_MOUSE

Protein

Glutamate receptor ionotropic, delta-2

Gene

Grid2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.

    GO - Molecular functioni

    1. extracellular-glutamate-gated ion channel activity Source: RefGenome
    2. ionotropic glutamate receptor activity Source: RefGenome
    3. PDZ domain binding Source: BHF-UCL
    4. protein binding Source: IntAct
    5. scaffold protein binding Source: BHF-UCL

    GO - Biological processi

    1. cellular protein localization Source: BHF-UCL
    2. cerebellar granule cell differentiation Source: BHF-UCL
    3. heterophilic cell-cell adhesion Source: BHF-UCL
    4. ionotropic glutamate receptor signaling pathway Source: RefGenome
    5. ion transmembrane transport Source: RefGenome
    6. prepulse inhibition Source: MGI
    7. regulation of excitatory postsynaptic membrane potential Source: MGI
    8. regulation of neuron apoptotic process Source: MGI
    9. regulation of neuron projection development Source: MGI
    10. synaptic transmission, glutamatergic Source: MGI

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Protein family/group databases

    TCDBi1.A.10.1.8. the glutamate-gated ion channel (gic) family of neurotransmitter receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate receptor ionotropic, delta-2
    Short name:
    GluD2
    Short name:
    GluR delta-2 subunit
    Gene namesi
    Name:Grid2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:95813. Grid2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. dendritic spine Source: BHF-UCL
    3. ionotropic glutamate receptor complex Source: BHF-UCL
    4. membrane Source: MGI
    5. plasma membrane Source: BHF-UCL
    6. postsynaptic membrane Source: BHF-UCL
    7. synapse Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Grid2 are the cause of the Lurcher phenotype. Heterozygous animals display a characteristic swaying of the hind quarters and jerky up and down movements following cerebellar Purkinje cell degeneration during postnatal development. Homozygous animals die shortly after birth because of a massive loss of midbrain and hindbrain neurons during late embryogenesis.

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi920 – 9201S → A: Abolishes interaction with SHANK1 and SHANK2. 1 Publication

    Keywords - Diseasei

    Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 1007984Glutamate receptor ionotropic, delta-2PRO_0000011565Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ61625.
    PaxDbiQ61625.
    PRIDEiQ61625.

    PTM databases

    PhosphoSiteiQ61625.

    Expressioni

    Tissue specificityi

    Expressed selectively in cerebellar Purkinje cells where it is localized in dendritic spines.2 Publications

    Gene expression databases

    BgeeiQ61625.
    CleanExiMM_GRID2.
    GenevestigatoriQ61625.

    Interactioni

    Subunit structurei

    Interacts with AIP1 and AP4M1. Interacts with EML2 By similarity. Interacts with BECN1, GOPC, GRID2IP, SHANK1 and SHANK2. Interacts with CBLN1 and CBLN2, but not with CBLN4. CBLN1-binding is calcium-independent.By similarity6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cbln1Q9R1718EBI-2794106,EBI-2794140
    GopcQ8BH605EBI-2794106,EBI-296357

    Protein-protein interaction databases

    BioGridi200062. 3 interactions.
    IntActiQ61625. 37 interactions.
    MINTiMINT-126960.
    STRINGi10090.ENSMUSP00000093536.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61625.
    SMRiQ61625. Positions 26-426, 441-809.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 566543ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini588 – 63548CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini657 – 830174ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini852 – 1007156CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei567 – 58721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei636 – 65621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei831 – 85121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni921 – 99171Interaction with AP4M1By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1005 – 10073PDZ-binding

    Domaini

    The PDZ-binding motif mediates interaction with GOPC.1 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG276334.
    GeneTreeiENSGT00740000115107.
    HOGENOMiHOG000264260.
    HOVERGENiHBG051840.
    InParanoidiA4QPG1.
    KOiK05207.
    OMAiHGNYAFV.
    OrthoDBiEOG7KQ20V.
    PhylomeDBiQ61625.
    TreeFamiTF352434.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    IPR001638. SBP_bac_3.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF00497. SBP_bac_3. 1 hit.
    [Graphical view]
    PRINTSiPR00177. NMDARECEPTOR.
    SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61625-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVFPLLLFL SFCWSRTWDL ATADSIIHIG AIFDESAKKD DEVFRTAVGD     50
    LNQNEEILQT EKITFSVTFV DGNNPFQAVQ EACELMNQGI LALVSSIGCT 100
    SAGSLQSLAD AMHIPHLFIQ RSTAGTPRSG CGLTRSNRND DYTLSVRPPV 150
    YLNEVILRVV TEYAWQKFII FYDSEYDIRG IQEFLDKVSQ QGMDVALQKV 200
    ENNINKMITT LFDTMRIEEL NRYRDTLRRA ILVMNPATAK SFISEVVETN 250
    LVAFDCHWII INEEINDVDV QELVRRSIGR LTIIRQTFPV PQNISQRCFR 300
    GNHRISSSLC DPKDPFAQNM EISNLYIYDT VLLLANAFHK KLEDRKWHSM 350
    ASLSCIRKNS KPWQGGRSML ETIKKGGVNG LTGDLEFGEN GGNPNVHFEI 400
    LGTNYGEELG RGVRKLGCWN PVTGLNGSLT DKKLENNMRG VVLRVVTVLE 450
    EPFVMVSENV LGKPKKYQGF SIDVLDALSN YLGFNYEIYV APDHKYGSPQ 500
    EDGTWNGLVG ELVFKRADIG ISALTITPDR ENVVDFTTRY MDYSVGVLLR 550
    RAEKTVDMFA CLAPFDLSLW ACIAGTVLLV GLLVYLLNWL NPPRLQMGSM 600
    TSTTLYNSMW FVYGSFVQQG GEVPYTTLAT RMMMGAWWLF ALIVISSYTA 650
    NLAAFLTITR IESSIQSLQD LSKQTDIPYG TVLDSAVYQH VRMKGLNPFE 700
    RDSMYSQMWR MINRSNGSEN NVLESQAGIQ KVKYGNYAFV WDAAVLEYVA 750
    INDPDCSFYT VGNTVADRGY GIALQHGSPY RDVFSQRILE LQQSGDMDIL 800
    KHKWWPKNGQ CDLYSSVDAK QKGGALDIKS LAGVFCILAA GIVLSCLIAV 850
    LETWWSRRKG SRVPSKEDDK EIDLEHLHRR VNSLCTDDDS PHKQFSTSSI 900
    DLTPLDIDTL PTRQALEQIS DFRNTHITTT TFIPEQIQTL SRTLSAKAAS 950
    GFAFGSVPEH RTGPFRHRAP NGGFFRSPIK TMSSIPYQPT PTLGLNLGND 1000
    PDRGTSI 1007
    Length:1,007
    Mass (Da):113,082
    Last modified:November 1, 1996 - v1
    Checksum:iA456166CC782A44B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti654 – 6541A → T in Lurcher. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13266 mRNA. Translation: BAA02524.1.
    BC139823 mRNA. Translation: AAI39824.1.
    CCDSiCCDS20202.1.
    PIRiPN0156.
    RefSeqiNP_032193.1. NM_008167.2.
    UniGeneiMm.439651.
    Mm.447390.

    Genome annotation databases

    EnsembliENSMUST00000095852; ENSMUSP00000093536; ENSMUSG00000071424.
    GeneIDi14804.
    KEGGimmu:14804.
    UCSCiuc009cdz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13266 mRNA. Translation: BAA02524.1 .
    BC139823 mRNA. Translation: AAI39824.1 .
    CCDSi CCDS20202.1.
    PIRi PN0156.
    RefSeqi NP_032193.1. NM_008167.2.
    UniGenei Mm.439651.
    Mm.447390.

    3D structure databases

    ProteinModelPortali Q61625.
    SMRi Q61625. Positions 26-426, 441-809.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200062. 3 interactions.
    IntActi Q61625. 37 interactions.
    MINTi MINT-126960.
    STRINGi 10090.ENSMUSP00000093536.

    Chemistry

    BindingDBi Q61625.
    GuidetoPHARMACOLOGYi 449.

    Protein family/group databases

    TCDBi 1.A.10.1.8. the glutamate-gated ion channel (gic) family of neurotransmitter receptors.

    PTM databases

    PhosphoSitei Q61625.

    Proteomic databases

    MaxQBi Q61625.
    PaxDbi Q61625.
    PRIDEi Q61625.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000095852 ; ENSMUSP00000093536 ; ENSMUSG00000071424 .
    GeneIDi 14804.
    KEGGi mmu:14804.
    UCSCi uc009cdz.1. mouse.

    Organism-specific databases

    CTDi 2895.
    MGIi MGI:95813. Grid2.

    Phylogenomic databases

    eggNOGi NOG276334.
    GeneTreei ENSGT00740000115107.
    HOGENOMi HOG000264260.
    HOVERGENi HBG051840.
    InParanoidi A4QPG1.
    KOi K05207.
    OMAi HGNYAFV.
    OrthoDBi EOG7KQ20V.
    PhylomeDBi Q61625.
    TreeFami TF352434.

    Miscellaneous databases

    ChiTaRSi GRID2. mouse.
    NextBioi 286973.
    PROi Q61625.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61625.
    CleanExi MM_GRID2.
    Genevestigatori Q61625.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    IPR001638. SBP_bac_3.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF00497. SBP_bac_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00177. NMDARECEPTOR.
    SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Selective expression of the glutamate receptor channel delta 2 subunit in cerebellar Purkinje cells."
      Araki K., Meguro H., Kushiya E., Takayama C., Inoue Y., Mishina M.
      Biochem. Biophys. Res. Commun. 197:1267-1276(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: ICR.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice."
      Yue Z., Horton A., Bravin M., DeJager P.L., Selimi F., Heintz N.
      Neuron 35:921-933(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GOPC AND BECN1, DOMAIN.
    4. "Direct interaction of GluRdelta2 with Shank scaffold proteins in cerebellar Purkinje cells."
      Uemura T., Mori H., Mishina M.
      Mol. Cell. Neurosci. 26:330-341(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHANK1 AND SHANK2, MUTAGENESIS OF SER-920, TISSUE SPECIFICITY.
    5. "Binding of glutamate receptor delta2 to its scaffold protein, Delphilin, is regulated by PKA."
      Sonoda T., Mochizuki C., Yamashita T., Watanabe-Kaneko K., Miyagi Y., Shigeri Y., Yazama F., Okuda K., Kawamoto S.
      Biochem. Biophys. Res. Commun. 350:748-752(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRID2IP.
    6. "Cbln1 is a ligand for an orphan glutamate receptor delta2, a bidirectional synapse organizer."
      Matsuda K., Miura E., Miyazaki T., Kakegawa W., Emi K., Narumi S., Fukazawa Y., Ito-Ishida A., Kondo T., Shigemoto R., Watanabe M., Yuzaki M.
      Science 328:363-368(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBLN1.
    7. "Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions."
      Matsuda K., Yuzaki M.
      Eur. J. Neurosci. 33:1447-1461(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBLN1.
    8. "The Cbln family of proteins interact with multiple signaling pathways."
      Wei P., Pattarini R., Rong Y., Guo H., Bansal P.K., Kusnoor S.V., Deutch A.Y., Parris J., Morgan J.I.
      J. Neurochem. 121:717-729(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBLN1 AND CBLN2.
    9. "Neurodegeneration in Lurcher mice caused by mutation in delta2 glutamate receptor gene."
      Zuo J., De Jager P.L., Takahashi K.A., Jiang W., Linden D.J., Heintz N.
      Nature 388:769-773(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT LURCHER THR-654.
      Tissue: Purkinje cell.

    Entry informationi

    Entry nameiGRID2_MOUSE
    AccessioniPrimary (citable) accession number: Q61625
    Secondary accession number(s): A4QPG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3