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Protein

Glutamate receptor ionotropic, delta-2

Gene

Grid2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.

GO - Molecular functioni

  • extracellular-glutamate-gated ion channel activity Source: GO_Central
  • ionotropic glutamate receptor activity Source: GO_Central
  • PDZ domain binding Source: BHF-UCL
  • scaffold protein binding Source: BHF-UCL

GO - Biological processi

  • cellular protein localization Source: BHF-UCL
  • cerebellar granule cell differentiation Source: BHF-UCL
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: BHF-UCL
  • ionotropic glutamate receptor signaling pathway Source: GO_Central
  • ion transmembrane transport Source: GO_Central
  • prepulse inhibition Source: MGI
  • regulation of excitatory postsynaptic membrane potential Source: MGI
  • regulation of neuron apoptotic process Source: MGI
  • regulation of neuron projection development Source: MGI
  • synaptic transmission, glutamatergic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi1.A.10.1.8. the glutamate-gated ion channel (gic) family of neurotransmitter receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, delta-2
Short name:
GluD2
Short name:
GluR delta-2 subunit
Gene namesi
Name:Grid2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95813. Grid2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 566543ExtracellularSequence AnalysisAdd
BLAST
Transmembranei567 – 58721HelicalSequence AnalysisAdd
BLAST
Topological domaini588 – 63548CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei636 – 65621HelicalSequence AnalysisAdd
BLAST
Topological domaini657 – 830174ExtracellularSequence AnalysisAdd
BLAST
Transmembranei831 – 85121HelicalSequence AnalysisAdd
BLAST
Topological domaini852 – 1007156CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • dendrite Source: GO_Central
  • dendritic spine Source: BHF-UCL
  • ionotropic glutamate receptor complex Source: BHF-UCL
  • membrane Source: MGI
  • plasma membrane Source: BHF-UCL
  • postsynaptic membrane Source: BHF-UCL
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Defects in Grid2 are the cause of the Lurcher phenotype. Heterozygous animals display a characteristic swaying of the hind quarters and jerky up and down movements following cerebellar Purkinje cell degeneration during postnatal development. Homozygous animals die shortly after birth because of a massive loss of midbrain and hindbrain neurons during late embryogenesis.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi920 – 9201S → A: Abolishes interaction with SHANK1 and SHANK2. 1 Publication

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 1007984Glutamate receptor ionotropic, delta-2PRO_0000011565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ61625.
PaxDbiQ61625.
PRIDEiQ61625.

PTM databases

PhosphoSiteiQ61625.

Expressioni

Tissue specificityi

Expressed selectively in cerebellar Purkinje cells where it is localized in dendritic spines.2 Publications

Gene expression databases

BgeeiQ61625.
CleanExiMM_GRID2.
ExpressionAtlasiQ61625. baseline and differential.
GenevisibleiQ61625. MM.

Interactioni

Subunit structurei

Interacts with AIP1 and AP4M1. Interacts with EML2 (By similarity). Interacts with BECN1, GOPC, GRID2IP, SHANK1 and SHANK2. Interacts with CBLN1 and CBLN2, but not with CBLN4. CBLN1-binding is calcium-independent.By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cbln1Q9R1718EBI-2794106,EBI-2794140
GopcQ8BH605EBI-2794106,EBI-296357

Protein-protein interaction databases

BioGridi200062. 3 interactions.
IntActiQ61625. 37 interactions.
MINTiMINT-126960.
STRINGi10090.ENSMUSP00000093536.

Structurei

3D structure databases

ProteinModelPortaliQ61625.
SMRiQ61625. Positions 441-610, 658-806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni921 – 99171Interaction with AP4M1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1005 – 10073PDZ-binding

Domaini

The PDZ-binding motif mediates interaction with GOPC.1 Publication

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG276334.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000264260.
HOVERGENiHBG051840.
InParanoidiQ61625.
KOiK05207.
OMAiHGNYAFV.
OrthoDBiEOG7KQ20V.
PhylomeDBiQ61625.
TreeFamiTF352434.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61625-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVFPLLLFL SFCWSRTWDL ATADSIIHIG AIFDESAKKD DEVFRTAVGD
60 70 80 90 100
LNQNEEILQT EKITFSVTFV DGNNPFQAVQ EACELMNQGI LALVSSIGCT
110 120 130 140 150
SAGSLQSLAD AMHIPHLFIQ RSTAGTPRSG CGLTRSNRND DYTLSVRPPV
160 170 180 190 200
YLNEVILRVV TEYAWQKFII FYDSEYDIRG IQEFLDKVSQ QGMDVALQKV
210 220 230 240 250
ENNINKMITT LFDTMRIEEL NRYRDTLRRA ILVMNPATAK SFISEVVETN
260 270 280 290 300
LVAFDCHWII INEEINDVDV QELVRRSIGR LTIIRQTFPV PQNISQRCFR
310 320 330 340 350
GNHRISSSLC DPKDPFAQNM EISNLYIYDT VLLLANAFHK KLEDRKWHSM
360 370 380 390 400
ASLSCIRKNS KPWQGGRSML ETIKKGGVNG LTGDLEFGEN GGNPNVHFEI
410 420 430 440 450
LGTNYGEELG RGVRKLGCWN PVTGLNGSLT DKKLENNMRG VVLRVVTVLE
460 470 480 490 500
EPFVMVSENV LGKPKKYQGF SIDVLDALSN YLGFNYEIYV APDHKYGSPQ
510 520 530 540 550
EDGTWNGLVG ELVFKRADIG ISALTITPDR ENVVDFTTRY MDYSVGVLLR
560 570 580 590 600
RAEKTVDMFA CLAPFDLSLW ACIAGTVLLV GLLVYLLNWL NPPRLQMGSM
610 620 630 640 650
TSTTLYNSMW FVYGSFVQQG GEVPYTTLAT RMMMGAWWLF ALIVISSYTA
660 670 680 690 700
NLAAFLTITR IESSIQSLQD LSKQTDIPYG TVLDSAVYQH VRMKGLNPFE
710 720 730 740 750
RDSMYSQMWR MINRSNGSEN NVLESQAGIQ KVKYGNYAFV WDAAVLEYVA
760 770 780 790 800
INDPDCSFYT VGNTVADRGY GIALQHGSPY RDVFSQRILE LQQSGDMDIL
810 820 830 840 850
KHKWWPKNGQ CDLYSSVDAK QKGGALDIKS LAGVFCILAA GIVLSCLIAV
860 870 880 890 900
LETWWSRRKG SRVPSKEDDK EIDLEHLHRR VNSLCTDDDS PHKQFSTSSI
910 920 930 940 950
DLTPLDIDTL PTRQALEQIS DFRNTHITTT TFIPEQIQTL SRTLSAKAAS
960 970 980 990 1000
GFAFGSVPEH RTGPFRHRAP NGGFFRSPIK TMSSIPYQPT PTLGLNLGND

PDRGTSI
Length:1,007
Mass (Da):113,082
Last modified:November 1, 1996 - v1
Checksum:iA456166CC782A44B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti654 – 6541A → T in Lurcher. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13266 mRNA. Translation: BAA02524.1.
BC139823 mRNA. Translation: AAI39824.1.
CCDSiCCDS20202.1.
PIRiPN0156.
RefSeqiNP_032193.1. NM_008167.2.
UniGeneiMm.439651.
Mm.447390.

Genome annotation databases

EnsembliENSMUST00000095852; ENSMUSP00000093536; ENSMUSG00000071424.
GeneIDi14804.
KEGGimmu:14804.
UCSCiuc009cdz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13266 mRNA. Translation: BAA02524.1.
BC139823 mRNA. Translation: AAI39824.1.
CCDSiCCDS20202.1.
PIRiPN0156.
RefSeqiNP_032193.1. NM_008167.2.
UniGeneiMm.439651.
Mm.447390.

3D structure databases

ProteinModelPortaliQ61625.
SMRiQ61625. Positions 441-610, 658-806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200062. 3 interactions.
IntActiQ61625. 37 interactions.
MINTiMINT-126960.
STRINGi10090.ENSMUSP00000093536.

Chemistry

BindingDBiQ61625.
GuidetoPHARMACOLOGYi449.

Protein family/group databases

TCDBi1.A.10.1.8. the glutamate-gated ion channel (gic) family of neurotransmitter receptors.

PTM databases

PhosphoSiteiQ61625.

Proteomic databases

MaxQBiQ61625.
PaxDbiQ61625.
PRIDEiQ61625.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095852; ENSMUSP00000093536; ENSMUSG00000071424.
GeneIDi14804.
KEGGimmu:14804.
UCSCiuc009cdz.1. mouse.

Organism-specific databases

CTDi2895.
MGIiMGI:95813. Grid2.

Phylogenomic databases

eggNOGiNOG276334.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000264260.
HOVERGENiHBG051840.
InParanoidiQ61625.
KOiK05207.
OMAiHGNYAFV.
OrthoDBiEOG7KQ20V.
PhylomeDBiQ61625.
TreeFamiTF352434.

Miscellaneous databases

ChiTaRSiGrid2. mouse.
NextBioi286973.
PROiQ61625.
SOURCEiSearch...

Gene expression databases

BgeeiQ61625.
CleanExiMM_GRID2.
ExpressionAtlasiQ61625. baseline and differential.
GenevisibleiQ61625. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Selective expression of the glutamate receptor channel delta 2 subunit in cerebellar Purkinje cells."
    Araki K., Meguro H., Kushiya E., Takayama C., Inoue Y., Mishina M.
    Biochem. Biophys. Res. Commun. 197:1267-1276(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: ICR.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice."
    Yue Z., Horton A., Bravin M., DeJager P.L., Selimi F., Heintz N.
    Neuron 35:921-933(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOPC AND BECN1, DOMAIN.
  4. "Direct interaction of GluRdelta2 with Shank scaffold proteins in cerebellar Purkinje cells."
    Uemura T., Mori H., Mishina M.
    Mol. Cell. Neurosci. 26:330-341(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHANK1 AND SHANK2, MUTAGENESIS OF SER-920, TISSUE SPECIFICITY.
  5. "Binding of glutamate receptor delta2 to its scaffold protein, Delphilin, is regulated by PKA."
    Sonoda T., Mochizuki C., Yamashita T., Watanabe-Kaneko K., Miyagi Y., Shigeri Y., Yazama F., Okuda K., Kawamoto S.
    Biochem. Biophys. Res. Commun. 350:748-752(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRID2IP.
  6. "Cbln1 is a ligand for an orphan glutamate receptor delta2, a bidirectional synapse organizer."
    Matsuda K., Miura E., Miyazaki T., Kakegawa W., Emi K., Narumi S., Fukazawa Y., Ito-Ishida A., Kondo T., Shigemoto R., Watanabe M., Yuzaki M.
    Science 328:363-368(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBLN1.
  7. "Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions."
    Matsuda K., Yuzaki M.
    Eur. J. Neurosci. 33:1447-1461(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBLN1.
  8. "The Cbln family of proteins interact with multiple signaling pathways."
    Wei P., Pattarini R., Rong Y., Guo H., Bansal P.K., Kusnoor S.V., Deutch A.Y., Parris J., Morgan J.I.
    J. Neurochem. 121:717-729(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBLN1 AND CBLN2.
  9. "Neurodegeneration in Lurcher mice caused by mutation in delta2 glutamate receptor gene."
    Zuo J., De Jager P.L., Takahashi K.A., Jiang W., Linden D.J., Heintz N.
    Nature 388:769-773(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT LURCHER THR-654.
    Tissue: Purkinje cell.

Entry informationi

Entry nameiGRID2_MOUSE
AccessioniPrimary (citable) accession number: Q61625
Secondary accession number(s): A4QPG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.