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Q61624

- ZN148_MOUSE

UniProt

Q61624 - ZN148_MOUSE

Protein

Zinc finger protein 148

Gene

Znf148

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri171 – 19323C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri227 – 24923C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri255 – 27824C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. double-stranded DNA binding Source: Ensembl
    3. metal ion binding Source: UniProtKB-KW
    4. sequence-specific DNA binding transcription factor activity Source: MGI
    5. transcription regulatory region sequence-specific DNA binding Source: Ensembl

    GO - Biological processi

    1. gamete generation Source: MGI
    2. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    4. protein complex assembly Source: Ensembl
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein 148
    Alternative name(s):
    Beta enolase repressor factor 1
    G-rich box-binding protein
    Transcription factor BFCOL1
    Transcription factor ZBP-89
    Zinc finger DNA-binding protein 89
    Gene namesi
    Name:Znf148
    Synonyms:Zbp89, Zfp148
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1332234. Zfp148.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: Ensembl
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 794794Zinc finger protein 148PRO_0000047428Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei194 – 1941PhosphothreonineBy similarity
    Modified residuei250 – 2501PhosphoserineBy similarity
    Modified residuei306 – 3061PhosphoserineBy similarity
    Modified residuei412 – 4121PhosphoserineBy similarity
    Modified residuei607 – 6071N6-acetyllysineBy similarity
    Modified residuei665 – 6651PhosphoserineBy similarity
    Modified residuei784 – 7841PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ61624.
    PRIDEiQ61624.

    PTM databases

    PhosphoSiteiQ61624.

    Expressioni

    Tissue specificityi

    Strong expression detected in brain, lung, liver and kidney, with lower levels detected in spleen, skeletal muscle, testis and heart.1 Publication

    Developmental stagei

    Detected in embryos from E7 to E17. Expression decreases in developing skeletal muscles.2 Publications

    Gene expression databases

    ArrayExpressiQ61624.
    BgeeiQ61624.
    CleanExiMM_ZFP148.
    GenevestigatoriQ61624.

    Interactioni

    Subunit structurei

    Interacts with HNRNPDL By similarity. Interacts with the 5FMC complex; the interaction requires association with CHTOP.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi204640. 1 interaction.
    IntActiQ61624. 1 interaction.
    MINTiMINT-4140637.
    STRINGi10090.ENSMUSP00000087106.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61624.
    SMRiQ61624. Positions 138-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri171 – 19323C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri227 – 24923C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri255 – 27824C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00530000063238.
    HOGENOMiHOG000293204.
    HOVERGENiHBG054247.
    InParanoidiQ61624.
    OMAiNDAYLNN.
    OrthoDBiEOG76QFGM.
    PhylomeDBiQ61624.
    TreeFamiTF331779.

    Family and domain databases

    Gene3Di3.30.160.60. 4 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 4 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61624-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIDDKLEGL FLKCGGIDEM QSSRAMVVMG GVSGQSAVSG ELQESVLQDR    50
    SLPHQEILAA DEVLQESEMR QQDMISHDEL MVHEETVKND EEQMDTHERL 100
    PQGLQYALNV PISVKQEITF TDVSEQLMRD KKQVREPVDL QKKKKRKQRS 150
    PAKILTINED GSLGLKTPKS HVCEHCNAAF RTNYHLQRHV FIHTGEKPFQ 200
    CSQCDMRFIQ KYLLQRHEKI HTGEKPFRCD ECGMRFIQKY HMERHKRTHS 250
    GEKPYQCEYC LQYFSRTDRV LKHKRMCHEN HDKKLNRCAI KGGLLTSEED 300
    SGFSTSPKDN SLPKKKRQKT EKKSSGMDKE SVLDKSDLKK DKNDYLPLYS 350
    SSTKVKDEYM VAEYAVEMPH SSVGGSHLED ASGEIHPPKL VLKKINSKRS 400
    LKQPLEQSQT ISPLSSYEDS KVSKYAFELV DKQALLDSEG SADIDQVDNL 450
    QEGPSKPVHS STNYDDAMQF LKKKRYLQAA SNNSREYALN VGTIASQPSV 500
    TQAAVASVID ESTTASILDS QALNVEIKSN HDKNVIPDEV LQTLLDHYSH 550
    KPNGQHEISF SVADTEVTSS ISINSSDVPE VTQSENVGSS SQASSSDKAN 600
    MLQEYSKFLQ QALDRTSQND AYLNSPSLNF VTDNQTLPNP PAFSSIDKQV 650
    YAAMPINSFR SGMNSPLRTT PDKSHFGLIV GDSQHPFPFS GDETNHASAT 700
    STADFLDQVT SQKKAEAQPV HQAYQMSSFE QPFRAPYHGS RAGIATQFST 750
    ANGQVNLRGP GTSAEFSEFP LVNVNDNRAG MTSSPDATTG QTFG 794
    Length:794
    Mass (Da):88,751
    Last modified:June 1, 1998 - v2
    Checksum:i4CB1C2A1822703FD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti283 – 2831K → N in AAB38507. (PubMed:9030551)Curated
    Sequence conflicti314 – 3141K → P in AAB38507. (PubMed:9030551)Curated
    Sequence conflicti319 – 3191K → Q in AAB38507. (PubMed:9030551)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98096 mRNA. Translation: CAA66725.1.
    BC026144 mRNA. Translation: AAH26144.1.
    U80078 mRNA. Translation: AAB38507.1.
    CCDSiCCDS28130.1.
    RefSeqiNP_035879.1. NM_011749.4.
    XP_006522165.1. XM_006522102.1.
    UniGeneiMm.392667.

    Genome annotation databases

    EnsembliENSMUST00000089677; ENSMUSP00000087106; ENSMUSG00000022811.
    ENSMUST00000165418; ENSMUSP00000126338; ENSMUSG00000022811.
    GeneIDi22661.
    KEGGimmu:22661.
    UCSCiuc007zab.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98096 mRNA. Translation: CAA66725.1 .
    BC026144 mRNA. Translation: AAH26144.1 .
    U80078 mRNA. Translation: AAB38507.1 .
    CCDSi CCDS28130.1.
    RefSeqi NP_035879.1. NM_011749.4.
    XP_006522165.1. XM_006522102.1.
    UniGenei Mm.392667.

    3D structure databases

    ProteinModelPortali Q61624.
    SMRi Q61624. Positions 138-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204640. 1 interaction.
    IntActi Q61624. 1 interaction.
    MINTi MINT-4140637.
    STRINGi 10090.ENSMUSP00000087106.

    PTM databases

    PhosphoSitei Q61624.

    Proteomic databases

    PaxDbi Q61624.
    PRIDEi Q61624.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000089677 ; ENSMUSP00000087106 ; ENSMUSG00000022811 .
    ENSMUST00000165418 ; ENSMUSP00000126338 ; ENSMUSG00000022811 .
    GeneIDi 22661.
    KEGGi mmu:22661.
    UCSCi uc007zab.1. mouse.

    Organism-specific databases

    CTDi 22661.
    MGIi MGI:1332234. Zfp148.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00530000063238.
    HOGENOMi HOG000293204.
    HOVERGENi HBG054247.
    InParanoidi Q61624.
    OMAi NDAYLNN.
    OrthoDBi EOG76QFGM.
    PhylomeDBi Q61624.
    TreeFami TF331779.

    Miscellaneous databases

    ChiTaRSi ZNF148. mouse.
    NextBioi 303071.
    PROi Q61624.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61624.
    Bgeei Q61624.
    CleanExi MM_ZFP148.
    Genevestigatori Q61624.

    Family and domain databases

    Gene3Di 3.30.160.60. 4 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 4 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Negative regulation of beta enolase gene transcription in embryonic muscle is dependent upon a zinc finger factor that binds to the G-rich box within the muscle-specific enhancer."
      Passantino R., Antona V., Barbieri G., Rubino P., Melchionna R., Cossu G., Feo S., Giallongo A.
      J. Biol. Chem. 273:484-494(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: CD-1.
      Tissue: Limb.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    3. "Cloning and characterization of a transcription factor that binds to the proximal promoters of the two mouse type I collagen genes."
      Hasegawa T., Takeuchi A., Miyaishi O., Isobe K., de Crombrugghe B.
      J. Biol. Chem. 272:4915-4923(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-794.
    4. "ZBP-89, a Kruppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter."
      Merchant J.L., Iyer G.R., Taylor B.R., Kitchen J.R., Mortensen E.R., Wang Z., Flintoft R.J., Michel J., Bassel-Duby R.
      Mol. Cell. Biol. 16:6644-6653(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
      Tissue: Pituitary adenoma.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation."
      Fanis P., Gillemans N., Aghajanirefah A., Pourfarzad F., Demmers J., Esteghamat F., Vadlamudi R.K., Grosveld F., Philipsen S., van Dijk T.B.
      Mol. Cell. Proteomics 11:1263-1273(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE 5FMC COMPLEX AND CHTOP, SUMOYLATION, DESUMOYLATION BY SENP3.

    Entry informationi

    Entry nameiZN148_MOUSE
    AccessioniPrimary (citable) accession number: Q61624
    Secondary accession number(s): P97475
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3