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Q61624

- ZN148_MOUSE

UniProt

Q61624 - ZN148_MOUSE

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Protein

Zinc finger protein 148

Gene

Znf148

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri171 – 19323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri227 – 24923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri255 – 27824C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. double-stranded DNA binding Source: Ensembl
  3. metal ion binding Source: UniProtKB-KW
  4. sequence-specific DNA binding transcription factor activity Source: MGI
  5. transcription regulatory region sequence-specific DNA binding Source: Ensembl

GO - Biological processi

  1. gamete generation Source: MGI
  2. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  4. protein complex assembly Source: Ensembl
  5. substantia nigra development Source: Ensembl
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 148
Alternative name(s):
Beta enolase repressor factor 1
G-rich box-binding protein
Transcription factor BFCOL1
Transcription factor ZBP-89
Zinc finger DNA-binding protein 89
Gene namesi
Name:Znf148
Synonyms:Zbp89, Zfp148
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1332234. Zfp148.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 794794Zinc finger protein 148PRO_0000047428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei194 – 1941PhosphothreonineBy similarity
Modified residuei250 – 2501PhosphoserineBy similarity
Modified residuei306 – 3061PhosphoserineBy similarity
Modified residuei412 – 4121PhosphoserineBy similarity
Modified residuei607 – 6071N6-acetyllysineBy similarity
Modified residuei665 – 6651PhosphoserineBy similarity
Modified residuei784 – 7841PhosphoserineBy similarity

Post-translational modificationi

Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ61624.
PaxDbiQ61624.
PRIDEiQ61624.

PTM databases

PhosphoSiteiQ61624.

Expressioni

Tissue specificityi

Strong expression detected in brain, lung, liver and kidney, with lower levels detected in spleen, skeletal muscle, testis and heart.1 Publication

Developmental stagei

Detected in embryos from E7 to E17. Expression decreases in developing skeletal muscles.2 Publications

Gene expression databases

BgeeiQ61624.
CleanExiMM_ZFP148.
ExpressionAtlasiQ61624. baseline and differential.
GenevestigatoriQ61624.

Interactioni

Subunit structurei

Interacts with HNRNPDL (By similarity). Interacts with the 5FMC complex; the interaction requires association with CHTOP.By similarity1 Publication

Protein-protein interaction databases

BioGridi204640. 1 interaction.
IntActiQ61624. 1 interaction.
MINTiMINT-4140637.
STRINGi10090.ENSMUSP00000087106.

Structurei

3D structure databases

ProteinModelPortaliQ61624.
SMRiQ61624. Positions 162-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri171 – 19323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri227 – 24923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri255 – 27824C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00530000063238.
HOGENOMiHOG000293204.
HOVERGENiHBG054247.
InParanoidiQ61624.
OMAiNDAYLNN.
OrthoDBiEOG76QFGM.
PhylomeDBiQ61624.
TreeFamiTF331779.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61624-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNIDDKLEGL FLKCGGIDEM QSSRAMVVMG GVSGQSAVSG ELQESVLQDR
60 70 80 90 100
SLPHQEILAA DEVLQESEMR QQDMISHDEL MVHEETVKND EEQMDTHERL
110 120 130 140 150
PQGLQYALNV PISVKQEITF TDVSEQLMRD KKQVREPVDL QKKKKRKQRS
160 170 180 190 200
PAKILTINED GSLGLKTPKS HVCEHCNAAF RTNYHLQRHV FIHTGEKPFQ
210 220 230 240 250
CSQCDMRFIQ KYLLQRHEKI HTGEKPFRCD ECGMRFIQKY HMERHKRTHS
260 270 280 290 300
GEKPYQCEYC LQYFSRTDRV LKHKRMCHEN HDKKLNRCAI KGGLLTSEED
310 320 330 340 350
SGFSTSPKDN SLPKKKRQKT EKKSSGMDKE SVLDKSDLKK DKNDYLPLYS
360 370 380 390 400
SSTKVKDEYM VAEYAVEMPH SSVGGSHLED ASGEIHPPKL VLKKINSKRS
410 420 430 440 450
LKQPLEQSQT ISPLSSYEDS KVSKYAFELV DKQALLDSEG SADIDQVDNL
460 470 480 490 500
QEGPSKPVHS STNYDDAMQF LKKKRYLQAA SNNSREYALN VGTIASQPSV
510 520 530 540 550
TQAAVASVID ESTTASILDS QALNVEIKSN HDKNVIPDEV LQTLLDHYSH
560 570 580 590 600
KPNGQHEISF SVADTEVTSS ISINSSDVPE VTQSENVGSS SQASSSDKAN
610 620 630 640 650
MLQEYSKFLQ QALDRTSQND AYLNSPSLNF VTDNQTLPNP PAFSSIDKQV
660 670 680 690 700
YAAMPINSFR SGMNSPLRTT PDKSHFGLIV GDSQHPFPFS GDETNHASAT
710 720 730 740 750
STADFLDQVT SQKKAEAQPV HQAYQMSSFE QPFRAPYHGS RAGIATQFST
760 770 780 790
ANGQVNLRGP GTSAEFSEFP LVNVNDNRAG MTSSPDATTG QTFG
Length:794
Mass (Da):88,751
Last modified:June 1, 1998 - v2
Checksum:i4CB1C2A1822703FD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti283 – 2831K → N in AAB38507. (PubMed:9030551)Curated
Sequence conflicti314 – 3141K → P in AAB38507. (PubMed:9030551)Curated
Sequence conflicti319 – 3191K → Q in AAB38507. (PubMed:9030551)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98096 mRNA. Translation: CAA66725.1.
BC026144 mRNA. Translation: AAH26144.1.
U80078 mRNA. Translation: AAB38507.1.
CCDSiCCDS28130.1.
RefSeqiNP_035879.1. NM_011749.4.
XP_006522165.1. XM_006522102.1.
UniGeneiMm.392667.

Genome annotation databases

EnsembliENSMUST00000089677; ENSMUSP00000087106; ENSMUSG00000022811.
ENSMUST00000165418; ENSMUSP00000126338; ENSMUSG00000022811.
GeneIDi22661.
KEGGimmu:22661.
UCSCiuc007zab.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98096 mRNA. Translation: CAA66725.1 .
BC026144 mRNA. Translation: AAH26144.1 .
U80078 mRNA. Translation: AAB38507.1 .
CCDSi CCDS28130.1.
RefSeqi NP_035879.1. NM_011749.4.
XP_006522165.1. XM_006522102.1.
UniGenei Mm.392667.

3D structure databases

ProteinModelPortali Q61624.
SMRi Q61624. Positions 162-296.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204640. 1 interaction.
IntActi Q61624. 1 interaction.
MINTi MINT-4140637.
STRINGi 10090.ENSMUSP00000087106.

PTM databases

PhosphoSitei Q61624.

Proteomic databases

MaxQBi Q61624.
PaxDbi Q61624.
PRIDEi Q61624.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000089677 ; ENSMUSP00000087106 ; ENSMUSG00000022811 .
ENSMUST00000165418 ; ENSMUSP00000126338 ; ENSMUSG00000022811 .
GeneIDi 22661.
KEGGi mmu:22661.
UCSCi uc007zab.1. mouse.

Organism-specific databases

CTDi 22661.
MGIi MGI:1332234. Zfp148.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00530000063238.
HOGENOMi HOG000293204.
HOVERGENi HBG054247.
InParanoidi Q61624.
OMAi NDAYLNN.
OrthoDBi EOG76QFGM.
PhylomeDBi Q61624.
TreeFami TF331779.

Miscellaneous databases

NextBioi 303071.
PROi Q61624.
SOURCEi Search...

Gene expression databases

Bgeei Q61624.
CleanExi MM_ZFP148.
ExpressionAtlasi Q61624. baseline and differential.
Genevestigatori Q61624.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 4 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Negative regulation of beta enolase gene transcription in embryonic muscle is dependent upon a zinc finger factor that binds to the G-rich box within the muscle-specific enhancer."
    Passantino R., Antona V., Barbieri G., Rubino P., Melchionna R., Cossu G., Feo S., Giallongo A.
    J. Biol. Chem. 273:484-494(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: CD-1.
    Tissue: Limb.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  3. "Cloning and characterization of a transcription factor that binds to the proximal promoters of the two mouse type I collagen genes."
    Hasegawa T., Takeuchi A., Miyaishi O., Isobe K., de Crombrugghe B.
    J. Biol. Chem. 272:4915-4923(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-794.
  4. "ZBP-89, a Kruppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter."
    Merchant J.L., Iyer G.R., Taylor B.R., Kitchen J.R., Mortensen E.R., Wang Z., Flintoft R.J., Michel J., Bassel-Duby R.
    Mol. Cell. Biol. 16:6644-6653(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
    Tissue: Pituitary adenoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation."
    Fanis P., Gillemans N., Aghajanirefah A., Pourfarzad F., Demmers J., Esteghamat F., Vadlamudi R.K., Grosveld F., Philipsen S., van Dijk T.B.
    Mol. Cell. Proteomics 11:1263-1273(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE 5FMC COMPLEX AND CHTOP, SUMOYLATION, DESUMOYLATION BY SENP3.

Entry informationi

Entry nameiZN148_MOUSE
AccessioniPrimary (citable) accession number: Q61624
Secondary accession number(s): P97475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3