Q61618 (AA3R_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine receptor A3

Alternative name(s):
A3AR

Gene names

Name:	Adora3
Synonyms:	Gpcr2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylation on Thr-317 and Thr-318 may be crucial for rapid desensitization. Phosphorylation on Thr-317 may be necessary for phosphorylation on Thr-318 to occur By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled adenosine receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Adenosine receptor A3		PRO_0000069011
Regions
	Topological domain	1 – 15	15	Extracellular By similarity
	Transmembrane	16 – 38	23	Helical; Name=1; By similarity
	Topological domain	39 – 49	11	Cytoplasmic By similarity
	Transmembrane	50 – 73	24	Helical; Name=2; By similarity
	Topological domain	74 – 85	12	Extracellular By similarity
	Transmembrane	86 – 107	22	Helical; Name=3; By similarity
	Topological domain	108 – 127	20	Cytoplasmic By similarity
	Transmembrane	128 – 149	22	Helical; Name=4; By similarity
	Topological domain	150 – 178	29	Extracellular By similarity
	Transmembrane	179 – 199	21	Helical; Name=5; By similarity
	Topological domain	200 – 232	33	Cytoplasmic By similarity
	Transmembrane	233 – 256	24	Helical; Name=6; By similarity
	Topological domain	257 – 262	6	Extracellular By similarity
	Transmembrane	263 – 285	23	Helical; Name=7; By similarity
	Topological domain	286 – 319	34	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	306	1	Phosphothreonine By similarity
	Modified residue	317	1	Phosphothreonine By similarity
	Modified residue	318	1	Phosphothreonine By similarity
	Lipidation	304	1	S-palmitoyl cysteine Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Disulfide bond	84 ↔ 167		By similarity
Experimental info
	Sequence conflict	184	1	I → V in AAA16851. Ref.2
	Sequence conflict	196	1	I → V in AAA16851. Ref.2
	Sequence conflict	214	1	T → S in AAA16851. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61618 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: 6AD7F46A9F03D9CC	FASTA	319	36,449
	10 20 30 40 50 60 MEADNTTETD WLNITYITME AAIGLCAVVG NMLVIWVVKL NPTLRTTTVY FIVSLALADI 70 80 90 100 110 120 AVGVLVIPLA IAVSLQVKMH FYACLFMSCV LLIFTHASIM SLLAIAVHRY LRVKLTVRYR 130 140 150 160 170 180 TVTTQRRIWL FLGLCWLVSF LVGLTPMFGW NRKATLASSQ NSSTLLCHFR SVVSLDYMVF 190 200 210 220 230 240 FSFITWILVP LVVMCIIYLD IFYIIRNKLS QNLTGFRETR AFYGREFKTA KSLFLVLFLF 250 260 270 280 290 300 ALCWLPLSII NFVSYFDVKI PDVAMCLGIL LSHANSMMNP IVYACKIKKF KETYFLILRA 310 VRLCQTSDSL DSNMEQTTE

References

[1]	"Characterization of the mouse A3 adenosine receptor gene: exon/intron organization and promoter activity." Zhao Z., Francis C., Ravid K. Genomics 57:152-155(1999) [PubMed: 10191095] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors." Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A. Genomics 18:175-184(1993) [PubMed: 8288218] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 111-239. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069778 Genomic DNA. Translation: AAC82643.1. L20331 mRNA. Translation: AAA16851.1.
IPI	IPI00123499.
PIR	B48909.
UniGene	Mm.235024.
3D structure databases
ProteinModelPortal	Q61618.
SMR	Q61618. Positions 14-316.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61618.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q61618.
Proteomic databases
PRIDE	Q61618.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:104847. Adora3.
Phylogenomic databases
HOGENOM	HBG445348.
HOVERGEN	HBG106962.
InParanoid	Q61618.
OrthoDB	EOG4MKNH1.
Gene expression databases
ArrayExpress	Q61618.
Bgee	Q61618.
CleanEx	MM_ADORA3.
Genevestigator	Q61618.
GermOnline	ENSMUSG00000000562. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR000466. Adeno_A3_rcpt. IPR001634. Adenosn_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00555. ADENOSINEA3R. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

AA3R_MOUSE

Accession

Primary (citable) accession number: Q61618
Secondary accession number(s): Q9R202

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program