Reviewed, UniProtKB/Swiss-Prot Q61609 (S20A1_MOUSE)

Names and origin

Protein names

Recommended name:
    Sodium-dependent phosphate transporter 1
Alternative name(s):
    Solute carrier family 20 member 1
    Phosphate transporter 1
    PiT-1
    Gibbon ape leukemia virus receptor 1
      Short name=GLVR-1
    Leukemia virus receptor 1 homolog

Gene names

Name:	Slc20a1
Synonyms:	Glvr1, Pit1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor but do not confer infection susceptibility to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B). Ref.1 Ref.7 Ref.9 Ref.10 Ref.11
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Ubiquitously expressed. Ref.10
Developmental stage	Detected at E17 of embryonic development in a subpopulation of early hypertrophic chondrocytes in bone, but not when fully differentiated. Ref.10
Induction	By growth hormone. Ref.9
Domain	Region A does not confer susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.
Sequence similarities	Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. [View classification]
Sequence caution	The sequence BAC29234.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention. The sequence differs at the 3'end due to intron retention.

Ontologies

Keywords
Biological process	Phosphate transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Receptor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	phosphate transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	inorganic phosphate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: UniProtKB-KW symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 681	681	Sodium-dependent phosphate transporter 1		PRO_0000080772
Regions
	Topological domain	1 – 24	24	Extracellular Potential
	Transmembrane	25 – 45	21	Potential
	Topological domain	46 – 65	20	Cytoplasmic Potential
	Transmembrane	66 – 86	21	Potential
	Topological domain	87 – 105	19	Extracellular Potential
	Transmembrane	106 – 126	21	Potential
	Topological domain	127 – 161	35	Cytoplasmic Potential
	Transmembrane	162 – 182	21	Potential
	Topological domain	183 – 200	18	Extracellular Potential
	Transmembrane	201 – 221	21	Potential
	Topological domain	222 – 233	12	Cytoplasmic Potential
	Transmembrane	234 – 254	21	Potential
	Topological domain	255 – 513	259	Extracellular Potential
	Transmembrane	514 – 534	21	Potential
	Topological domain	535 – 560	26	Cytoplasmic Potential
	Transmembrane	561 – 581	21	Potential
	Topological domain	582 – 601	20	Extracellular Potential
	Transmembrane	602 – 622	21	Potential
	Topological domain	623 – 651	29	Cytoplasmic Potential
	Transmembrane	652 – 672	21	Potential
	Topological domain	673 – 681	9	Extracellular Potential
	Region	553 – 560	8	A
Amino acid modifications
	Modified residue	268	1	Phosphoserine
	Modified residue	269	1	Phosphoserine Ref.12
	Modified residue	273	1	Phosphoserine Ref.12
Experimental info
	Mutagenesis	553 – 560	8	KQEASTKA → DTGDVSSKV: Confers virus infectibility. Ref.8
	Mutagenesis	553 – 555	3	KQE → DTGD: Confers virus infectibility. Ref.8
	Mutagenesis	553	1	K → DT: Confers virus infectibility. Ref.8
	Sequence conflict	190	1	R → H Ref.2
	Sequence conflict	368	1	T → A Ref.2
	Sequence conflict	446	1	D → E Ref.2
	Sequence conflict	490	1	E → G in AAH15085. Ref.6
	Sequence conflict	568	1	L → P in BAE33298. Ref.4
	Sequence conflict	569	1	Y → T Ref.2
	Sequence conflict	639	1	R → I Ref.2
	Sequence conflict	672	1	A → AA Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61609-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F337B0982B5A297D	FASTA	681	74,153
	10 20 30 40 50 60 MESTVATITS TLAAVTASAP PKYDNLWMLI LGFIIAFVLA FSVGANDVAN SFGTAVGSGV 70 80 90 100 110 120 VTLKQACILA SIFETVGSAL LGAKVSETIR NGLIDVELYN ETQDLLMAGS VSAMFGSAVW 130 140 150 160 170 180 QLVASFLKLP ISGTHCIVGA TIGFSLVANG QKGVKWSELI KIVMSWFVSP LLSGIMSGIL 190 200 210 220 230 240 FFLVRAFILR KADPVPNGLR ALPIFYACTI GINLFSIMYT GAPLLGFDKL PLWGTILISV 250 260 270 280 290 300 GCAVFCALIV WFFVCPRMKR KIEREVKSSP SESPLMEKKS NLKEDHEETK MAPGDVEHRN 310 320 330 340 350 360 PVSEVVCATG PLRAVVEERT VSFKLGDLEE APERERLPMD LKEETSIDST INGAVQLPNG 370 380 390 400 410 420 NLVQFSQTVS NQINSSGHYQ YHTVHKDSGL YKELLHKLHL AKVGDCMGDS GDKPLRRNNS 430 440 450 460 470 480 YTSYTMAICG MPLDSFRAKE GEQKGDEMET LTWPNADTKK RIRMDSYTSY CNAVSDLHSE 490 500 510 520 530 540 SEMDMSVKAE MGLGDRKGSS GSLEEWYDQD KPEVSLLFQF LQILTACFGS FAHGGNDVSN 550 560 570 580 590 600 AIGPLVALYL VYKQEASTKA ATPIWLLLYG GVGICMGLWV WGRRVIQTMG KDLTPITPSS 610 620 630 640 650 660 GFSIELASAL TVVIASNIGL PISTTHCKVG SVVSVGWLRS KKAVDWRLFR NIFMAWFVTV 670 680 PISGVISAAI MAVFKYIILP V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus." Johann S.V., Gibbons J.J., O'Hara B. J. Virol. 66:1635-1640(1992) [PubMed: 1531369] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Comparison of cDNAs encoding the gibbon ape leukaemia virus receptor from susceptible and non-susceptible murine cells." Wilson C.A., Farrell K.B., Eiden M.V. J. Gen. Virol. 75:1901-1908(1994) [PubMed: 8046392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure of the murine Pit1 phosphate transporter/retrovirus receptor gene and functional characterization of its promoter region." Palmer G., Manen D., Bonjour J.-P., Caverzasio J. Gene 244:35-45(2000) [PubMed: 10689185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD.
[5]	The mouse genome sequencing consortium Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
[7]	"Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus." Takeuchi Y., Vile R.G., Simpson G., O'Hara B., Collins M.K., Weiss R.A. J. Virol. 66:1219-1222(1992) [PubMed: 1309898] [Abstract] Cited for: FUNCTION.
[8]	"Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species." Johann S.V., van Zeijl M., Cekleniak J., O'Hara B. J. Virol. 67:6733-6736(1993) [PubMed: 8411375] [Abstract] Cited for: MUTAGENESIS OF 553-LYS--ALA-560; 553-LYS--GLU-555 AND LYS-553, REGION.
[9]	"Differential expression, abundance, and regulation of Na+-phosphate cotransporter genes in murine kidney." Tenenhouse H.S., Roy S., Martel J., Gauthier C. Am. J. Physiol. 275:F527-F534(1998) [PubMed: 9755124] [Abstract] Cited for: FUNCTION, INDUCTION.
[10]	"In vivo expression of transcripts encoding the Glvr-1 phosphate transporter/retrovirus receptor during bone development." Palmer G., Zhao J., Bonjour J.-P., Hofstetter W., Caverzasio J. Bone 24:1-7(1999) [PubMed: 9916777] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[11]	"Reassessing the role of region A in Pit1-mediated viral entry." Farrell K.B., Russ J.L., Murthy R.K., Eiden M.V. J. Virol. 76:7683-7693(2002) [PubMed: 12097582] [Abstract] Cited for: FUNCTION, REGION.
[12]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed: 15572359] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269 AND SER-273, MASS SPECTROMETRY. Tissue: Brain.
[13]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268; SER-269 AND SER-273, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73696 mRNA. Translation: AAA74887.1. AF172634 , AF172628, AF172629, AF172630, AF172631, AF172632, AF172633 Genomic DNA. Translation: AAF45041.1. AK035898 mRNA. Translation: BAC29234.1. Sequence problems. AK155506 mRNA. Translation: BAE33298.1. AL772347 Genomic DNA. Translation: CAM15802.1. BC015085 mRNA. Translation: AAH15085.1.
IPI	IPI00322626.
PIR	S27868.
RefSeq	NP_056562.1.
UniGene	Mm.272675 Mm.457995
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q61609.
Proteomic databases
PRIDE	Q61609.
Genome annotation databases
Ensembl	ENSMUSG00000027397. Mus musculus. [Contig view]
GeneID	20515.
KEGG	mmu:20515.
Organism-specific databases
MGI	MGI:108392. Slc20a1.
Phylogenomic databases
HOGENOM	Q61609.
HOVERGEN	Q61609.
OMA	Q61609. VEMYNST.
Gene expression databases
ArrayExpress	Q61609.
Bgee	Q61609.
CleanEx	MM_SLC20A1.
GermOnline	ENSMUSG00000027397. Mus musculus.
Family and domain databases
InterPro	IPR001204. Phos_transporter. [Graphical view]
PANTHER	PTHR11101. Phos_transporter. 1 hit.
Pfam	PF01384. PHO4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	298723.
SOURCE	Search...

Entry information

Entry name

S20A1_MOUSE

Accession

Primary (citable) accession number: Q61609
Secondary accession number(s): A2AKR9 , Q3U244, Q8CBJ1, Q91YQ9, Q9QVW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)