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Q61609 (S20A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-dependent phosphate transporter 1
Alternative name(s):
Gibbon ape leukemia virus receptor 1
Short name=GLVR-1
Leukemia virus receptor 1 homolog
Phosphate transporter 1
Short name=PiT-1
Solute carrier family 20 member 1
Gene names
Name:Slc20a1
Synonyms:Glvr1, Pit1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length681 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor but do not confer infection susceptibility to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B). Ref.1 Ref.7 Ref.9 Ref.10 Ref.11

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Ubiquitously expressed. Ref.10

Developmental stage

Detected at E17 of embryonic development in a subpopulation of early hypertrophic chondrocytes in bone, but not when fully differentiated. Ref.10

Induction

By growth hormone. Ref.9

Domain

Region A does not confer susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.

Sequence similarities

Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. [View classification]

Sequence caution

The sequence BAC29234.1 differs from that shown. Reason: Intron retention. The sequence differs at the 3'end due to intron retention.

Ontologies

Keywords
   Biological processPhosphate transport
Symport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay. Source: MGI

   Molecular functioninorganic phosphate transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 681681Sodium-dependent phosphate transporter 1
PRO_0000080772

Regions

Topological domain1 – 2424Extracellular Potential
Transmembrane25 – 4521Helical; Potential
Topological domain46 – 6520Cytoplasmic Potential
Transmembrane66 – 8621Helical; Potential
Topological domain87 – 10519Extracellular Potential
Transmembrane106 – 12621Helical; Potential
Topological domain127 – 16135Cytoplasmic Potential
Transmembrane162 – 18221Helical; Potential
Topological domain183 – 20018Extracellular Potential
Transmembrane201 – 22121Helical; Potential
Topological domain222 – 23312Cytoplasmic Potential
Transmembrane234 – 25421Helical; Potential
Topological domain255 – 513259Extracellular Potential
Transmembrane514 – 53421Helical; Potential
Topological domain535 – 56026Cytoplasmic Potential
Transmembrane561 – 58121Helical; Potential
Topological domain582 – 60120Extracellular Potential
Transmembrane602 – 62221Helical; Potential
Topological domain623 – 65129Cytoplasmic Potential
Transmembrane652 – 67221Helical; Potential
Topological domain673 – 6819Extracellular Potential
Region553 – 5608A

Amino acid modifications

Modified residue2681Phosphoserine Ref.13
Modified residue2691Phosphoserine Ref.12 Ref.13
Modified residue2711Phosphoserine By similarity
Modified residue2731Phosphoserine Ref.12 Ref.13

Experimental info

Mutagenesis553 – 5608KQEASTKA → DTGDVSSKV: Confers virus infectibility. Ref.8
Mutagenesis553 – 5553KQE → DTGD: Confers virus infectibility. Ref.8
Mutagenesis5531K → DT: Confers virus infectibility. Ref.8
Sequence conflict1901R → H Ref.2
Sequence conflict3681T → A Ref.2
Sequence conflict4461D → E Ref.2
Sequence conflict4901E → G in AAH15085. Ref.6
Sequence conflict5681L → P in BAE33298. Ref.4
Sequence conflict5691Y → T Ref.2
Sequence conflict6391R → I Ref.2
Sequence conflict6721A → AA Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q61609 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F337B0982B5A297D

FASTA68174,153
        10         20         30         40         50         60 
MESTVATITS TLAAVTASAP PKYDNLWMLI LGFIIAFVLA FSVGANDVAN SFGTAVGSGV 

        70         80         90        100        110        120 
VTLKQACILA SIFETVGSAL LGAKVSETIR NGLIDVELYN ETQDLLMAGS VSAMFGSAVW 

       130        140        150        160        170        180 
QLVASFLKLP ISGTHCIVGA TIGFSLVANG QKGVKWSELI KIVMSWFVSP LLSGIMSGIL 

       190        200        210        220        230        240 
FFLVRAFILR KADPVPNGLR ALPIFYACTI GINLFSIMYT GAPLLGFDKL PLWGTILISV 

       250        260        270        280        290        300 
GCAVFCALIV WFFVCPRMKR KIEREVKSSP SESPLMEKKS NLKEDHEETK MAPGDVEHRN 

       310        320        330        340        350        360 
PVSEVVCATG PLRAVVEERT VSFKLGDLEE APERERLPMD LKEETSIDST INGAVQLPNG 

       370        380        390        400        410        420 
NLVQFSQTVS NQINSSGHYQ YHTVHKDSGL YKELLHKLHL AKVGDCMGDS GDKPLRRNNS 

       430        440        450        460        470        480 
YTSYTMAICG MPLDSFRAKE GEQKGDEMET LTWPNADTKK RIRMDSYTSY CNAVSDLHSE 

       490        500        510        520        530        540 
SEMDMSVKAE MGLGDRKGSS GSLEEWYDQD KPEVSLLFQF LQILTACFGS FAHGGNDVSN 

       550        560        570        580        590        600 
AIGPLVALYL VYKQEASTKA ATPIWLLLYG GVGICMGLWV WGRRVIQTMG KDLTPITPSS 

       610        620        630        640        650        660 
GFSIELASAL TVVIASNIGL PISTTHCKVG SVVSVGWLRS KKAVDWRLFR NIFMAWFVTV 

       670        680 
PISGVISAAI MAVFKYIILP V

« Hide

References

« Hide 'large scale' references
[1]"GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus."
Johann S.V., Gibbons J.J., O'Hara B.
J. Virol. 66:1635-1640(1992) [PubMed: 1531369] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"Comparison of cDNAs encoding the gibbon ape leukaemia virus receptor from susceptible and non-susceptible murine cells."
Wilson C.A., Farrell K.B., Eiden M.V.
J. Gen. Virol. 75:1901-1908(1994) [PubMed: 8046392] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure of the murine Pit1 phosphate transporter/retrovirus receptor gene and functional characterization of its promoter region."
Palmer G., Manen D., Bonjour J.-P., Caverzasio J.
Gene 244:35-45(2000) [PubMed: 10689185] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
[7]"Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus."
Takeuchi Y., Vile R.G., Simpson G., O'Hara B., Collins M.K., Weiss R.A.
J. Virol. 66:1219-1222(1992) [PubMed: 1309898] [Abstract]
Cited for: FUNCTION.
[8]"Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species."
Johann S.V., van Zeijl M., Cekleniak J., O'Hara B.
J. Virol. 67:6733-6736(1993) [PubMed: 8411375] [Abstract]
Cited for: MUTAGENESIS OF 553-LYS--ALA-560; 553-LYS--GLU-555 AND LYS-553, REGION.
[9]"Differential expression, abundance, and regulation of Na+-phosphate cotransporter genes in murine kidney."
Tenenhouse H.S., Roy S., Martel J., Gauthier C.
Am. J. Physiol. 275:F527-F534(1998) [PubMed: 9755124] [Abstract]
Cited for: FUNCTION, INDUCTION.
[10]"In vivo expression of transcripts encoding the Glvr-1 phosphate transporter/retrovirus receptor during bone development."
Palmer G., Zhao J., Bonjour J.-P., Hofstetter W., Caverzasio J.
Bone 24:1-7(1999) [PubMed: 9916777] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[11]"Reassessing the role of region A in Pit1-mediated viral entry."
Farrell K.B., Russ J.L., Murthy R.K., Eiden M.V.
J. Virol. 76:7683-7693(2002) [PubMed: 12097582] [Abstract]
Cited for: FUNCTION, REGION.
[12]"Proteomic analysis of in vivo phosphorylated synaptic proteins."
Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G.
J. Biol. Chem. 280:5972-5982(2005) [PubMed: 15572359] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269 AND SER-273, MASS SPECTROMETRY.
Tissue: Forebrain.
[13]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268; SER-269 AND SER-273, MASS SPECTROMETRY.
Tissue: Macrophage.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M73696 mRNA. Translation: AAA74887.1.
AF172634 expand/collapse EMBL AC list , AF172628, AF172629, AF172630, AF172631, AF172632, AF172633 Genomic DNA. Translation: AAF45041.1.
AK035898 mRNA. Translation: BAC29234.1. Sequence problems.
AK155506 mRNA. Translation: BAE33298.1.
AL772347 Genomic DNA. Translation: CAM15802.1.
BC015085 mRNA. Translation: AAH15085.1.
IPIIPI00322626.
PIRS27868.
RefSeqNP_001153065.1. NM_001159593.1.
NP_056562.1. NM_015747.2.
UniGeneMm.272675.

3D structure databases

ProteinModelPortalQ61609.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ61609.

PTM databases

PhosphoSiteQ61609.

Proteomic databases

PRIDEQ61609.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028880; ENSMUSP00000028880; ENSMUSG00000027397.
ENSMUST00000110315; ENSMUSP00000105944; ENSMUSG00000027397.
GeneID20515.
KEGGmmu:20515.
UCSCuc008mhl.2. mouse.

Organism-specific databases

CTD6574.
MGIMGI:108392. Slc20a1.

Phylogenomic databases

eggNOGroNOG09188.
GeneTreeENSGT00390000014879.
HOGENOMHBG681698.
HOVERGENHBG053358.
InParanoidQ61609.
OMAVEMYNST.
OrthoDBEOG428212.
PhylomeDBQ61609.

Gene expression databases

ArrayExpressQ61609.
BgeeQ61609.
CleanExMM_SLC20A1.
GenevestigatorQ61609.
GermOnlineENSMUSG00000027397. Mus musculus.

Family and domain databases

InterProIPR001204. Phos_transporter.
[Graphical view]
KOK03306.
PANTHERPTHR11101. Phos_transporter. 1 hit.
PfamPF01384. PHO4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298723.
SOURCESearch...

Entry information

Entry nameS20A1_MOUSE
AccessionPrimary (citable) accession number: Q61609
Secondary accession number(s): A2AKR9 expand/collapse secondary AC list , Q3U244, Q8CBJ1, Q91YQ9, Q9QVW6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1996
Last modified: November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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