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Q61606

- GLR_MOUSE

UniProt

Q61606 - GLR_MOUSE

Protein

Glucagon receptor

Gene

Gcgr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.2 Publications

    GO - Molecular functioni

    1. glucagon receptor activity Source: UniProtKB
    2. peptide hormone binding Source: Ensembl

    GO - Biological processi

    1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: Ensembl
    2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
    3. cellular response to glucagon stimulus Source: UniProtKB
    4. exocytosis Source: Ensembl
    5. glucose homeostasis Source: UniProtKB
    6. hormone-mediated signaling pathway Source: Ensembl
    7. regulation of glycogen metabolic process Source: UniProtKB
    8. response to starvation Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.
    REACT_210399. Glucagon signaling in metabolic regulation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucagon receptor
    Short name:
    GL-R
    Gene namesi
    Name:Gcgr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:99572. Gcgr.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Is rapidly internalized after ligand-binding.By similarity

    GO - Cellular componenti

    1. endosome Source: Ensembl
    2. integral component of plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are born at the expected Mendelian rate. They display pancreas islet and alpha-cell hyperplasia and increased glucagon levels, but normal insulin levels. Mice display low blood glucose levels combined with increased hepatic glycogen levels. They develop severe hypoglycemia after prolonged fasting. Mutant mice are fertile, but the females produce only few pups; half of the embryos die before birth, and liveborn pups do not survive more than one day. These pups are much smaller than their littermates and exhibit severe hypoglycemia.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 485459Glucagon receptorPRO_0000012833Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 68By similarity
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi59 ↔ 101By similarity
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi82 ↔ 122By similarity
    Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ61606.

    PTM databases

    PhosphoSiteiQ61606.

    Expressioni

    Tissue specificityi

    Expressed predominantly in liver, kidney, adrenal, lung and stomach, while lower levels of expression are detected in brown and white adipose tissue, cerebellum, duodenum and heart.1 Publication

    Gene expression databases

    BgeeiQ61606.
    CleanExiMM_GCGR.
    GenevestigatoriQ61606.

    Interactioni

    Protein-protein interaction databases

    BioGridi199863. 1 interaction.
    STRINGi10090.ENSMUSP00000026119.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61606.
    SMRiQ61606. Positions 30-429.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 137111ExtracellularBy similarityAdd
    BLAST
    Topological domaini163 – 17412CytoplasmicBy similarityAdd
    BLAST
    Topological domaini200 – 22627ExtracellularBy similarityAdd
    BLAST
    Topological domaini251 – 26414CytoplasmicBy similarityAdd
    BLAST
    Topological domaini287 – 30418ExtracellularBy similarityAdd
    BLAST
    Topological domaini328 – 35124CytoplasmicBy similarityAdd
    BLAST
    Topological domaini371 – 38212ExtracellularBy similarityAdd
    BLAST
    Topological domaini404 – 48582CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei138 – 16225Helical; Name=1By similarityAdd
    BLAST
    Transmembranei175 – 19925Helical; Name=2By similarityAdd
    BLAST
    Transmembranei227 – 25024Helical; Name=3By similarityAdd
    BLAST
    Transmembranei265 – 28622Helical; Name=4By similarityAdd
    BLAST
    Transmembranei305 – 32723Helical; Name=5By similarityAdd
    BLAST
    Transmembranei352 – 37019Helical; Name=6By similarityAdd
    BLAST
    Transmembranei383 – 40321Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG263329.
    GeneTreeiENSGT00740000115231.
    HOGENOMiHOG000008250.
    HOVERGENiHBG008318.
    InParanoidiQ8K0B5.
    KOiK04583.
    OMAiLPWHHKV.
    OrthoDBiEOG7TF78W.
    TreeFamiTF315710.

    Family and domain databases

    InterProiIPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR003290. GPCR_2_GLP1/glucagon_rcpt.
    IPR003291. GPCR_2_glucagon_rcpt.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view]
    PfamiPF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view]
    PRINTSiPR01353. GLUCAGNFAMLY.
    PR01354. GLUCAGONR.
    PR00249. GPCRSECRETIN.
    SMARTiSM00008. HormR. 1 hit.
    [Graphical view]
    PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61606-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLTQLHCPH LLLLLLVLSC LPEAPSAQVM DFLFEKWKLY SDQCHHNLSL    50
    LPPPTELVCN RTFDKYSCWP DTPPNTTANI SCPWYLPWYH KVQHRLVFKR 100
    CGPDGQWVRG PRGQPWRNAS QCQLDDEEIE VQKGVAKMYS SQQVMYTVGY 150
    SLSLGALLLA LVILLGLRKL HCTRNYIHGN LFASFVLKAG SVLVIDWLLK 200
    TRYSQKIGDD LSVSVWLSDG AMAGCRVATV IMQYGIIANY CWLLVEGVYL 250
    YSLLSLATFS ERSFFSLYLG IGWGAPLLFV IPWVVVKCLF ENVQCWTSND 300
    NMGFWWILRI PVFLALLINF FIFVHIIHLL VAKLRAHQMH YADYKFRLAR 350
    STLTLIPLLG VHEVVFAFVT DEHAQGTLRS TKLFFDLFLS SFQGLLVAVL 400
    YCFLNKEVQA ELMRRWRQWQ EGKALQEERL ASSHGSHMAP AGPCHGDPCE 450
    KLQLMSAGSS SGTGCVPSME TSLASSLPRL ADSPT 485
    Length:485
    Mass (Da):54,929
    Last modified:July 27, 2011 - v2
    Checksum:i578EB30BF281E67A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651K → N in AAA88244. (PubMed:7590348)Curated
    Sequence conflicti89 – 891Y → C in AAA88244. (PubMed:7590348)Curated
    Sequence conflicti238 – 2381A → P in AAA88244. (PubMed:7590348)Curated
    Sequence conflicti325 – 3251H → R in AAB30529. 1 PublicationCurated
    Sequence conflicti328 – 3281H → Q in AAA88244. (PubMed:7590348)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38613 mRNA. Translation: AAA88244.1.
    AL669855 Genomic DNA. Translation: CAM27053.1.
    CH466558 Genomic DNA. Translation: EDL34761.1.
    BC031885 mRNA. Translation: AAH31885.1.
    BC057988 mRNA. Translation: AAH57988.1.
    S69384 mRNA. Translation: AAB30529.2.
    CCDSiCCDS25739.1.
    PIRiJC4363.
    RefSeqiNP_032127.2. NM_008101.2.
    UniGeneiMm.22329.

    Genome annotation databases

    EnsembliENSMUST00000026119; ENSMUSP00000026119; ENSMUSG00000025127.
    GeneIDi14527.
    KEGGimmu:14527.
    UCSCiuc007mtc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38613 mRNA. Translation: AAA88244.1 .
    AL669855 Genomic DNA. Translation: CAM27053.1 .
    CH466558 Genomic DNA. Translation: EDL34761.1 .
    BC031885 mRNA. Translation: AAH31885.1 .
    BC057988 mRNA. Translation: AAH57988.1 .
    S69384 mRNA. Translation: AAB30529.2 .
    CCDSi CCDS25739.1.
    PIRi JC4363.
    RefSeqi NP_032127.2. NM_008101.2.
    UniGenei Mm.22329.

    3D structure databases

    ProteinModelPortali Q61606.
    SMRi Q61606. Positions 30-429.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199863. 1 interaction.
    STRINGi 10090.ENSMUSP00000026119.

    Chemistry

    BindingDBi Q61606.
    ChEMBLi CHEMBL4773.
    GuidetoPHARMACOLOGYi 251.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q61606.

    Proteomic databases

    PRIDEi Q61606.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026119 ; ENSMUSP00000026119 ; ENSMUSG00000025127 .
    GeneIDi 14527.
    KEGGi mmu:14527.
    UCSCi uc007mtc.2. mouse.

    Organism-specific databases

    CTDi 2642.
    MGIi MGI:99572. Gcgr.

    Phylogenomic databases

    eggNOGi NOG263329.
    GeneTreei ENSGT00740000115231.
    HOGENOMi HOG000008250.
    HOVERGENi HBG008318.
    InParanoidi Q8K0B5.
    KOi K04583.
    OMAi LPWHHKV.
    OrthoDBi EOG7TF78W.
    TreeFami TF315710.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.
    REACT_210399. Glucagon signaling in metabolic regulation.

    Miscellaneous databases

    ChiTaRSi GCGR. mouse.
    NextBioi 286172.
    PROi Q61606.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61606.
    CleanExi MM_GCGR.
    Genevestigatori Q61606.

    Family and domain databases

    InterProi IPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR003290. GPCR_2_GLP1/glucagon_rcpt.
    IPR003291. GPCR_2_glucagon_rcpt.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view ]
    Pfami PF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view ]
    PRINTSi PR01353. GLUCAGNFAMLY.
    PR01354. GLUCAGONR.
    PR00249. GPCRSECRETIN.
    SMARTi SM00008. HormR. 1 hit.
    [Graphical view ]
    PROSITEi PS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of the murine glucagon receptor-encoding gene."
      Burcelin R., Li J., Charron M.J.
      Gene 164:305-310(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    5. "Divergent tissue-specific and developmental expression of receptors for glucagon and glucagon-like peptide-1 in the mouse."
      Campos R.V., Lee Y.C., Drucker D.J.
      Endocrinology 134:2156-2164(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 324-458.
      Tissue: Brain.
    6. Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
    7. "Ablation of the glucagon receptor gene increases fetal lethality and produces alterations in islet development and maturation."
      Vuguin P.M., Kedees M.H., Cui L., Guz Y., Gelling R.W., Nejathaim M., Charron M.J., Teitelman G.
      Endocrinology 147:3995-4006(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    8. "The glucagon receptor is required for the adaptive metabolic response to fasting."
      Longuet C., Sinclair E.M., Maida A., Baggio L.L., Maziarz M., Charron M.J., Drucker D.J.
      Cell Metab. 8:359-371(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.

    Entry informationi

    Entry nameiGLR_MOUSE
    AccessioniPrimary (citable) accession number: Q61606
    Secondary accession number(s): Q63960, Q8K0B5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3