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Q61606 (GLR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucagon receptor

Short name=GL-R
Gene names
Name:Gcgr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system. Ref.6 Ref.8

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Is rapidly internalized after ligand-binding By similarity. Ref.6

Tissue specificity

Expressed predominantly in liver, kidney, adrenal, lung and stomach, while lower levels of expression are detected in brown and white adipose tissue, cerebellum, duodenum and heart. Ref.1

Post-translational modification

Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding By similarity.

Disruption phenotype

Mice are born at the expected Mendelian rate. They display pancreas islet and alpha-cell hyperplasia and increased glucagon levels, but normal insulin levels. Mice display low blood glucose levels combined with increased hepatic glycogen levels. They develop severe hypoglycemia after prolonged fasting. Mutant mice are fertile, but the females produce only few pups; half of the embryos die before birth, and liveborn pups do not survive more than one day. These pups are much smaller than their littermates and exhibit severe hypoglycemia. Ref.6 Ref.7 Ref.8

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 485459Glucagon receptor
PRO_0000012833

Regions

Topological domain27 – 137111Extracellular By similarity
Transmembrane138 – 16225Helical; Name=1; By similarity
Topological domain163 – 17412Cytoplasmic By similarity
Transmembrane175 – 19925Helical; Name=2; By similarity
Topological domain200 – 22627Extracellular By similarity
Transmembrane227 – 25024Helical; Name=3; By similarity
Topological domain251 – 26414Cytoplasmic By similarity
Transmembrane265 – 28622Helical; Name=4; By similarity
Topological domain287 – 30418Extracellular By similarity
Transmembrane305 – 32723Helical; Name=5; By similarity
Topological domain328 – 35124Cytoplasmic By similarity
Transmembrane352 – 37019Helical; Name=6; By similarity
Topological domain371 – 38212Extracellular By similarity
Transmembrane383 – 40321Helical; Name=7; By similarity
Topological domain404 – 48582Cytoplasmic By similarity

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation1181N-linked (GlcNAc...) Potential
Disulfide bond44 ↔ 68 By similarity
Disulfide bond59 ↔ 101 By similarity
Disulfide bond82 ↔ 122 By similarity

Experimental info

Sequence conflict651K → N in AAA88244. Ref.1
Sequence conflict891Y → C in AAA88244. Ref.1
Sequence conflict2381A → P in AAA88244. Ref.1
Sequence conflict3251H → R in AAB30529. Ref.3
Sequence conflict3281H → Q in AAA88244. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61606 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 578EB30BF281E67A

FASTA48554,929
        10         20         30         40         50         60 
MPLTQLHCPH LLLLLLVLSC LPEAPSAQVM DFLFEKWKLY SDQCHHNLSL LPPPTELVCN 

        70         80         90        100        110        120 
RTFDKYSCWP DTPPNTTANI SCPWYLPWYH KVQHRLVFKR CGPDGQWVRG PRGQPWRNAS 

       130        140        150        160        170        180 
QCQLDDEEIE VQKGVAKMYS SQQVMYTVGY SLSLGALLLA LVILLGLRKL HCTRNYIHGN 

       190        200        210        220        230        240 
LFASFVLKAG SVLVIDWLLK TRYSQKIGDD LSVSVWLSDG AMAGCRVATV IMQYGIIANY 

       250        260        270        280        290        300 
CWLLVEGVYL YSLLSLATFS ERSFFSLYLG IGWGAPLLFV IPWVVVKCLF ENVQCWTSND 

       310        320        330        340        350        360 
NMGFWWILRI PVFLALLINF FIFVHIIHLL VAKLRAHQMH YADYKFRLAR STLTLIPLLG 

       370        380        390        400        410        420 
VHEVVFAFVT DEHAQGTLRS TKLFFDLFLS SFQGLLVAVL YCFLNKEVQA ELMRRWRQWQ 

       430        440        450        460        470        480 
EGKALQEERL ASSHGSHMAP AGPCHGDPCE KLQLMSAGSS SGTGCVPSME TSLASSLPRL 


ADSPT 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of the murine glucagon receptor-encoding gene."
Burcelin R., Li J., Charron M.J.
Gene 164:305-310(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[5]"Divergent tissue-specific and developmental expression of receptors for glucagon and glucagon-like peptide-1 in the mouse."
Campos R.V., Lee Y.C., Drucker D.J.
Endocrinology 134:2156-2164(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 324-458.
Tissue: Brain.
[6]"Lower blood glucose, hyperglucagonemia, and pancreatic alpha cell hyperplasia in glucagon receptor knockout mice."
Gelling R.W., Du X.Q., Dichmann D.S., Romer J., Huang H., Cui L., Obici S., Tang B., Holst J.J., Fledelius C., Johansen P.B., Rossetti L., Jelicks L.A., Serup P., Nishimura E., Charron M.J.
Proc. Natl. Acad. Sci. U.S.A. 100:1438-1443(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
[7]"Ablation of the glucagon receptor gene increases fetal lethality and produces alterations in islet development and maturation."
Vuguin P.M., Kedees M.H., Cui L., Guz Y., Gelling R.W., Nejathaim M., Charron M.J., Teitelman G.
Endocrinology 147:3995-4006(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[8]"The glucagon receptor is required for the adaptive metabolic response to fasting."
Longuet C., Sinclair E.M., Maida A., Baggio L.L., Maziarz M., Charron M.J., Drucker D.J.
Cell Metab. 8:359-371(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L38613 mRNA. Translation: AAA88244.1.
AL669855 Genomic DNA. Translation: CAM27053.1.
CH466558 Genomic DNA. Translation: EDL34761.1.
BC031885 mRNA. Translation: AAH31885.1.
BC057988 mRNA. Translation: AAH57988.1.
S69384 mRNA. Translation: AAB30529.2.
PIRJC4363.
RefSeqNP_032127.2. NM_008101.2.
UniGeneMm.22329.

3D structure databases

ProteinModelPortalQ61606.
SMRQ61606. Positions 30-429.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199863. 1 interaction.
STRING10090.ENSMUSP00000026119.

Chemistry

BindingDBQ61606.
ChEMBLCHEMBL4773.
GuidetoPHARMACOLOGY251.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ61606.

Proteomic databases

PRIDEQ61606.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026119; ENSMUSP00000026119; ENSMUSG00000025127.
GeneID14527.
KEGGmmu:14527.
UCSCuc007mtc.2. mouse.

Organism-specific databases

CTD2642.
MGIMGI:99572. Gcgr.

Phylogenomic databases

eggNOGNOG263329.
GeneTreeENSGT00740000115231.
HOGENOMHOG000008250.
HOVERGENHBG008318.
InParanoidQ8K0B5.
KOK04583.
OMALPWHHKV.
OrthoDBEOG7TF78W.
TreeFamTF315710.

Gene expression databases

BgeeQ61606.
CleanExMM_GCGR.
GenevestigatorQ61606.

Family and domain databases

InterProIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003290. GPCR_2_GLP1/glucagon_rcpt.
IPR003291. GPCR_2_glucagon_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PANTHERPTHR12011:SF71. PTHR12011:SF71. 1 hit.
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR01353. GLUCAGNFAMLY.
PR01354. GLUCAGONR.
PR00249. GPCRSECRETIN.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGCGR. mouse.
NextBio286172.
PROQ61606.
SOURCESearch...

Entry information

Entry nameGLR_MOUSE
AccessionPrimary (citable) accession number: Q61606
Secondary accession number(s): Q63960, Q8K0B5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries