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Q61606

- GLR_MOUSE

UniProt

Q61606 - GLR_MOUSE

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Protein

Glucagon receptor

Gene

Gcgr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.2 Publications

GO - Molecular functioni

  1. glucagon receptor activity Source: UniProtKB
  2. peptide hormone binding Source: Ensembl

GO - Biological processi

  1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: Ensembl
  2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
  3. cellular response to glucagon stimulus Source: UniProtKB
  4. exocytosis Source: Ensembl
  5. glucose homeostasis Source: UniProtKB
  6. hormone-mediated signaling pathway Source: Ensembl
  7. regulation of glycogen metabolic process Source: UniProtKB
  8. response to starvation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_210399. Glucagon signaling in metabolic regulation.
REACT_231304. Glucagon-type ligand receptors.
REACT_242871. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucagon receptor
Short name:
GL-R
Gene namesi
Name:Gcgr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:99572. Gcgr.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Is rapidly internalized after ligand-binding.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 137111ExtracellularBy similarityAdd
BLAST
Transmembranei138 – 16225Helical; Name=1By similarityAdd
BLAST
Topological domaini163 – 17412CytoplasmicBy similarityAdd
BLAST
Transmembranei175 – 19925Helical; Name=2By similarityAdd
BLAST
Topological domaini200 – 22627ExtracellularBy similarityAdd
BLAST
Transmembranei227 – 25024Helical; Name=3By similarityAdd
BLAST
Topological domaini251 – 26414CytoplasmicBy similarityAdd
BLAST
Transmembranei265 – 28622Helical; Name=4By similarityAdd
BLAST
Topological domaini287 – 30418ExtracellularBy similarityAdd
BLAST
Transmembranei305 – 32723Helical; Name=5By similarityAdd
BLAST
Topological domaini328 – 35124CytoplasmicBy similarityAdd
BLAST
Transmembranei352 – 37019Helical; Name=6By similarityAdd
BLAST
Topological domaini371 – 38212ExtracellularBy similarityAdd
BLAST
Transmembranei383 – 40321Helical; Name=7By similarityAdd
BLAST
Topological domaini404 – 48582CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. endosome Source: Ensembl
  2. integral component of plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate. They display pancreas islet and alpha-cell hyperplasia and increased glucagon levels, but normal insulin levels. Mice display low blood glucose levels combined with increased hepatic glycogen levels. They develop severe hypoglycemia after prolonged fasting. Mutant mice are fertile, but the females produce only few pups; half of the embryos die before birth, and liveborn pups do not survive more than one day. These pups are much smaller than their littermates and exhibit severe hypoglycemia.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 485459Glucagon receptorPRO_0000012833Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 68By similarity
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi59 ↔ 101By similarity
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi82 ↔ 122By similarity
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ61606.

PTM databases

PhosphoSiteiQ61606.

Expressioni

Tissue specificityi

Expressed predominantly in liver, kidney, adrenal, lung and stomach, while lower levels of expression are detected in brown and white adipose tissue, cerebellum, duodenum and heart.1 Publication

Gene expression databases

BgeeiQ61606.
CleanExiMM_GCGR.
GenevestigatoriQ61606.

Interactioni

Protein-protein interaction databases

BioGridi199863. 1 interaction.
STRINGi10090.ENSMUSP00000026119.

Structurei

3D structure databases

ProteinModelPortaliQ61606.
SMRiQ61606. Positions 30-429.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263329.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008250.
HOVERGENiHBG008318.
InParanoidiQ61606.
KOiK04583.
OMAiLPWHHKV.
OrthoDBiEOG7TF78W.
TreeFamiTF315710.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003290. GPCR_2_GLP1/glucagon_rcpt.
IPR003291. GPCR_2_glucagon_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01353. GLUCAGNFAMLY.
PR01354. GLUCAGONR.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61606-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLTQLHCPH LLLLLLVLSC LPEAPSAQVM DFLFEKWKLY SDQCHHNLSL
60 70 80 90 100
LPPPTELVCN RTFDKYSCWP DTPPNTTANI SCPWYLPWYH KVQHRLVFKR
110 120 130 140 150
CGPDGQWVRG PRGQPWRNAS QCQLDDEEIE VQKGVAKMYS SQQVMYTVGY
160 170 180 190 200
SLSLGALLLA LVILLGLRKL HCTRNYIHGN LFASFVLKAG SVLVIDWLLK
210 220 230 240 250
TRYSQKIGDD LSVSVWLSDG AMAGCRVATV IMQYGIIANY CWLLVEGVYL
260 270 280 290 300
YSLLSLATFS ERSFFSLYLG IGWGAPLLFV IPWVVVKCLF ENVQCWTSND
310 320 330 340 350
NMGFWWILRI PVFLALLINF FIFVHIIHLL VAKLRAHQMH YADYKFRLAR
360 370 380 390 400
STLTLIPLLG VHEVVFAFVT DEHAQGTLRS TKLFFDLFLS SFQGLLVAVL
410 420 430 440 450
YCFLNKEVQA ELMRRWRQWQ EGKALQEERL ASSHGSHMAP AGPCHGDPCE
460 470 480
KLQLMSAGSS SGTGCVPSME TSLASSLPRL ADSPT
Length:485
Mass (Da):54,929
Last modified:July 27, 2011 - v2
Checksum:i578EB30BF281E67A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651K → N in AAA88244. (PubMed:7590348)Curated
Sequence conflicti89 – 891Y → C in AAA88244. (PubMed:7590348)Curated
Sequence conflicti238 – 2381A → P in AAA88244. (PubMed:7590348)Curated
Sequence conflicti325 – 3251H → R in AAB30529. 1 PublicationCurated
Sequence conflicti328 – 3281H → Q in AAA88244. (PubMed:7590348)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38613 mRNA. Translation: AAA88244.1.
AL669855 Genomic DNA. Translation: CAM27053.1.
CH466558 Genomic DNA. Translation: EDL34761.1.
BC031885 mRNA. Translation: AAH31885.1.
BC057988 mRNA. Translation: AAH57988.1.
S69384 mRNA. Translation: AAB30529.2.
CCDSiCCDS25739.1.
PIRiJC4363.
RefSeqiNP_032127.2. NM_008101.2.
UniGeneiMm.22329.

Genome annotation databases

EnsembliENSMUST00000026119; ENSMUSP00000026119; ENSMUSG00000025127.
GeneIDi14527.
KEGGimmu:14527.
UCSCiuc007mtc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38613 mRNA. Translation: AAA88244.1 .
AL669855 Genomic DNA. Translation: CAM27053.1 .
CH466558 Genomic DNA. Translation: EDL34761.1 .
BC031885 mRNA. Translation: AAH31885.1 .
BC057988 mRNA. Translation: AAH57988.1 .
S69384 mRNA. Translation: AAB30529.2 .
CCDSi CCDS25739.1.
PIRi JC4363.
RefSeqi NP_032127.2. NM_008101.2.
UniGenei Mm.22329.

3D structure databases

ProteinModelPortali Q61606.
SMRi Q61606. Positions 30-429.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199863. 1 interaction.
STRINGi 10090.ENSMUSP00000026119.

Chemistry

BindingDBi Q61606.
ChEMBLi CHEMBL4773.
GuidetoPHARMACOLOGYi 251.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q61606.

Proteomic databases

PRIDEi Q61606.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026119 ; ENSMUSP00000026119 ; ENSMUSG00000025127 .
GeneIDi 14527.
KEGGi mmu:14527.
UCSCi uc007mtc.2. mouse.

Organism-specific databases

CTDi 2642.
MGIi MGI:99572. Gcgr.

Phylogenomic databases

eggNOGi NOG263329.
GeneTreei ENSGT00760000118800.
HOGENOMi HOG000008250.
HOVERGENi HBG008318.
InParanoidi Q61606.
KOi K04583.
OMAi LPWHHKV.
OrthoDBi EOG7TF78W.
TreeFami TF315710.

Enzyme and pathway databases

Reactomei REACT_207651. G alpha (q) signalling events.
REACT_210399. Glucagon signaling in metabolic regulation.
REACT_231304. Glucagon-type ligand receptors.
REACT_242871. G alpha (s) signalling events.

Miscellaneous databases

ChiTaRSi Gcgr. mouse.
NextBioi 286172.
PROi Q61606.
SOURCEi Search...

Gene expression databases

Bgeei Q61606.
CleanExi MM_GCGR.
Genevestigatori Q61606.

Family and domain databases

InterProi IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003290. GPCR_2_GLP1/glucagon_rcpt.
IPR003291. GPCR_2_glucagon_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view ]
Pfami PF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view ]
PRINTSi PR01353. GLUCAGNFAMLY.
PR01354. GLUCAGONR.
PR00249. GPCRSECRETIN.
SMARTi SM00008. HormR. 1 hit.
[Graphical view ]
PROSITEi PS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of the murine glucagon receptor-encoding gene."
    Burcelin R., Li J., Charron M.J.
    Gene 164:305-310(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  5. "Divergent tissue-specific and developmental expression of receptors for glucagon and glucagon-like peptide-1 in the mouse."
    Campos R.V., Lee Y.C., Drucker D.J.
    Endocrinology 134:2156-2164(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 324-458.
    Tissue: Brain.
  6. Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
  7. "Ablation of the glucagon receptor gene increases fetal lethality and produces alterations in islet development and maturation."
    Vuguin P.M., Kedees M.H., Cui L., Guz Y., Gelling R.W., Nejathaim M., Charron M.J., Teitelman G.
    Endocrinology 147:3995-4006(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  8. "The glucagon receptor is required for the adaptive metabolic response to fasting."
    Longuet C., Sinclair E.M., Maida A., Baggio L.L., Maziarz M., Charron M.J., Drucker D.J.
    Cell Metab. 8:359-371(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiGLR_MOUSE
AccessioniPrimary (citable) accession number: Q61606
Secondary accession number(s): Q63960, Q8K0B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3