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Protein

Glycine receptor subunit alpha-4

Gene

Glra4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine (PubMed:10762330). Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents (Probable).Curated1 Publication

Enzyme regulationi

Inhibited by strychnine (PubMed:10762330).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei295 – 2951Important for obstruction of the ion pore in the closed conformationBy similarity

GO - Molecular functioni

GO - Biological processi

  • chloride transmembrane transport Source: UniProtKB
  • neuropeptide signaling pathway Source: GO_Central
  • response to amino acid Source: UniProtKB
  • synaptic transmission, glycinergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-4
Gene namesi
Name:Glra4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95750. Glra4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 256229ExtracellularBy similarityAdd
BLAST
Transmembranei257 – 27822Helical; Name=1By similarityAdd
BLAST
Topological domaini279 – 2835CytoplasmicBy similarity
Transmembranei284 – 30421Helical; Name=2By similarityAdd
BLAST
Topological domaini305 – 31511ExtracellularBy similarityAdd
BLAST
Transmembranei316 – 33621Helical; Name=3By similarityAdd
BLAST
Topological domaini337 – 42387CytoplasmicBy similarityAdd
BLAST
Transmembranei424 – 44421Helical; Name=4By similarityAdd
BLAST
Topological domaini445 – 45612ExtracellularBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3500.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 456429Glycine receptor subunit alpha-4PRO_0000000422Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence analysis
Disulfide bondi171 ↔ 185By similarity
Disulfide bondi232 ↔ 243By similarity
Modified residuei368 – 3681PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ61603.
PRIDEiQ61603.

PTM databases

iPTMnetiQ61603.
PhosphoSiteiQ61603.

Expressioni

Tissue specificityi

Detected in the retina inner plexiform layer, especially at the border between layer three and four (at protein level) (PubMed:17154252).2 Publications

Gene expression databases

BgeeiENSMUSG00000018595.
CleanExiMM_GLRA4.
GenevisibleiQ61603. MM.

Interactioni

Subunit structurei

Homopentamer (in vitro). Heteropentamer composed of GLRA4 and GLRB.Curated

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018739.

Chemistry

BindingDBiQ61603.

Structurei

3D structure databases

ProteinModelPortaliQ61603.
SMRiQ61603. Positions 42-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 2416Strychnine-bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ61603.
KOiK05271.
OMAiARDGGPM.
OrthoDBiEOG091G0805.
PhylomeDBiQ61603.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLVPASLF LLLWTLPGKV LLSVALAKED VKSGLKGSQP MSPSDFLDKL
60 70 80 90 100
MGRTSGYDAR IRPNFKGPPV NVTCNIFINS FGSVTETTMD YRVNVFLRQQ
110 120 130 140 150
WNDPRLAYRE YPDDSLDLDP SMLDSIWKPD LFFANEKGAN FHEVTTDNKL
160 170 180 190 200
LRIFKNGNVL YSIRLTLILS CPMDLKNFPM DIQTCTMQLE SFGYTMNDLM
210 220 230 240 250
FEWLEDAPAV QVAEGLTLPQ FILRDEKDLG YCTKHYNTGK FTCIEVKFHL
260 270 280 290 300
ERQMGYYLIQ MYIPSLLIVI LSWVSFWINM DAAPARVGLG ITTVLTMTTQ
310 320 330 340 350
SSGSRASLPK VSYVKAIDIW MAVCLLFVFA ALLEYAAVNF VSRQHKEFMR
360 370 380 390 400
LRRRQRRQRM EEDIIRESRF YFRGYGLGHC LQARDGGPME GSSIYSPQPP
410 420 430 440 450
TPLLKEGETM RKLYVDRAKR IDTISRAVFP FTFLVFNIFY WVVYKVLRSE

DIHQAL
Length:456
Mass (Da):52,514
Last modified:October 25, 2005 - v3
Checksum:i4199CB8404E05BEA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191D → N in AAL69899 (Ref. 4) Curated
Sequence conflicti124 – 1241D → E in AAL69899 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ109809 mRNA. Translation: AAZ17380.1.
AL671887 Genomic DNA. Translation: CAM17223.1.
X75850
, X75851, X75852, X75853 Genomic DNA. Translation: CAA53468.1.
AF462147 mRNA. Translation: AAL69899.1.
CCDSiCCDS53195.1.
PIRiA49970.
RefSeqiNP_034427.2. NM_010297.2.
XP_006528569.1. XM_006528506.2.
XP_011246033.1. XM_011247731.1.
UniGeneiMm.215168.

Genome annotation databases

EnsembliENSMUST00000018739; ENSMUSP00000018739; ENSMUSG00000018595.
GeneIDi14657.
KEGGimmu:14657.
UCSCiuc009uiz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ109809 mRNA. Translation: AAZ17380.1.
AL671887 Genomic DNA. Translation: CAM17223.1.
X75850
, X75851, X75852, X75853 Genomic DNA. Translation: CAA53468.1.
AF462147 mRNA. Translation: AAL69899.1.
CCDSiCCDS53195.1.
PIRiA49970.
RefSeqiNP_034427.2. NM_010297.2.
XP_006528569.1. XM_006528506.2.
XP_011246033.1. XM_011247731.1.
UniGeneiMm.215168.

3D structure databases

ProteinModelPortaliQ61603.
SMRiQ61603. Positions 42-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018739.

Chemistry

BindingDBiQ61603.
ChEMBLiCHEMBL3500.

PTM databases

iPTMnetiQ61603.
PhosphoSiteiQ61603.

Proteomic databases

PaxDbiQ61603.
PRIDEiQ61603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018739; ENSMUSP00000018739; ENSMUSG00000018595.
GeneIDi14657.
KEGGimmu:14657.
UCSCiuc009uiz.2. mouse.

Organism-specific databases

CTDi441509.
MGIiMGI:95750. Glra4.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ61603.
KOiK05271.
OMAiARDGGPM.
OrthoDBiEOG091G0805.
PhylomeDBiQ61603.
TreeFamiTF315453.

Miscellaneous databases

PROiQ61603.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018595.
CleanExiMM_GLRA4.
GenevisibleiQ61603. MM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRA4_MOUSE
AccessioniPrimary (citable) accession number: Q61603
Secondary accession number(s): A2AEA9, Q45V76, Q8VHF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 25, 2005
Last modified: September 7, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.