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Q61603 (GLRA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine receptor subunit alpha-4
Gene names
Name:Glra4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). May play a role in the maturation of reproductive organs. Ref.1

Subunit structure

Pentamer composed of alpha and beta subunits By similarity.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Miscellaneous

The alpha subunit binds strychnine.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA4 sub-subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 456429Glycine receptor subunit alpha-4
PRO_0000000422

Regions

Topological domain28 – 257230Extracellular Potential
Transmembrane258 – 27821Helical; Potential
Topological domain279 – 31537Cytoplasmic Potential
Transmembrane316 – 33621Helical; Potential
Topological domain337 – 42690Extracellular Potential
Transmembrane427 – 44721Helical; Potential
Topological domain448 – 4569Cytoplasmic Potential

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential
Disulfide bond171 ↔ 185 By similarity
Disulfide bond232 ↔ 243 By similarity

Experimental info

Sequence conflict1191D → N in AAL69899. Ref.4
Sequence conflict1241D → E in AAL69899. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q61603 [UniParc].

Last modified October 25, 2005. Version 3.
Checksum: 4199CB8404E05BEA

FASTA45652,514
        10         20         30         40         50         60 
MTTLVPASLF LLLWTLPGKV LLSVALAKED VKSGLKGSQP MSPSDFLDKL MGRTSGYDAR 

        70         80         90        100        110        120 
IRPNFKGPPV NVTCNIFINS FGSVTETTMD YRVNVFLRQQ WNDPRLAYRE YPDDSLDLDP 

       130        140        150        160        170        180 
SMLDSIWKPD LFFANEKGAN FHEVTTDNKL LRIFKNGNVL YSIRLTLILS CPMDLKNFPM 

       190        200        210        220        230        240 
DIQTCTMQLE SFGYTMNDLM FEWLEDAPAV QVAEGLTLPQ FILRDEKDLG YCTKHYNTGK 

       250        260        270        280        290        300 
FTCIEVKFHL ERQMGYYLIQ MYIPSLLIVI LSWVSFWINM DAAPARVGLG ITTVLTMTTQ 

       310        320        330        340        350        360 
SSGSRASLPK VSYVKAIDIW MAVCLLFVFA ALLEYAAVNF VSRQHKEFMR LRRRQRRQRM 

       370        380        390        400        410        420 
EEDIIRESRF YFRGYGLGHC LQARDGGPME GSSIYSPQPP TPLLKEGETM RKLYVDRAKR 

       430        440        450 
IDTISRAVFP FTFLVFNIFY WVVYKVLRSE DIHQAL 

« Hide

References

« Hide 'large scale' references
[1]"Glycine receptors containing the alpha4 subunit in the embryonic sympathetic nervous system, spinal cord and male genital ridge."
Harvey R.J., Schmieden V., Von Holst A., Laube B., Rohrer H., Betz H.
Eur. J. Neurosci. 12:994-1001(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: C57BL/6J.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Structural analysis of mouse glycine receptor alpha subunit genes. Identification and chromosomal localization of a novel variant."
Matzenbach B., Maulet Y., Sefton L., Courtier B., Avner P., Guenet J.-L., Betz H.
J. Biol. Chem. 269:2607-2612(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-360.
Strain: BALB/c.
[4]"Localization of different glycine receptor isoforms in murine spinal cord."
Groemer T.-W., Becker C.-M., Becker K.
Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 41-456.
Strain: B6C3/Fe.
Tissue: Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ109809 mRNA. Translation: AAZ17380.1.
AL671887 Genomic DNA. Translation: CAM17223.1.
X75850 expand/collapse EMBL AC list , X75851, X75852, X75853 Genomic DNA. Translation: CAA53468.1.
AF462147 mRNA. Translation: AAL69899.1.
CCDSCCDS53195.1.
PIRA49970.
RefSeqNP_034427.2. NM_010297.2.
XP_006528569.1. XM_006528506.1.
UniGeneMm.215168.

3D structure databases

ProteinModelPortalQ61603.
SMRQ61603. Positions 54-451.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ61603.
ChEMBLCHEMBL3500.
GuidetoPHARMACOLOGY426.

PTM databases

PhosphoSiteQ61603.

Proteomic databases

PRIDEQ61603.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000018739; ENSMUSP00000018739; ENSMUSG00000018595.
GeneID14657.
KEGGmmu:14657.
UCSCuc009uiz.2. mouse.

Organism-specific databases

CTD441509.
MGIMGI:95750. Glra4.

Phylogenomic databases

eggNOGNOG265706.
GeneTreeENSGT00550000074453.
HOGENOMHOG000231336.
HOVERGENHBG051707.
InParanoidA2AEA9.
KOK05271.
OMASYREYPD.
OrthoDBEOG712TVZ.
PhylomeDBQ61603.
TreeFamTF315453.

Gene expression databases

BgeeQ61603.
CleanExMM_GLRA4.
GenevestigatorQ61603.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286518.
PROQ61603.
SOURCESearch...

Entry information

Entry nameGLRA4_MOUSE
AccessionPrimary (citable) accession number: Q61603
Secondary accession number(s): A2AEA9, Q45V76, Q8VHF3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 25, 2005
Last modified: July 9, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot