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Protein

Glycine receptor subunit alpha-4

Gene

Glra4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). May play a role in the maturation of reproductive organs.1 Publication

GO - Molecular functioni

  1. chloride channel activity Source: UniProtKB-KW
  2. extracellular ligand-gated ion channel activity Source: InterPro
  3. glycine binding Source: InterPro
  4. transmitter-gated ion channel activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-4
Gene namesi
Name:Glra4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95750. Glra4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 257230ExtracellularSequence AnalysisAdd
BLAST
Transmembranei258 – 27821HelicalSequence AnalysisAdd
BLAST
Topological domaini279 – 31537CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei316 – 33621HelicalSequence AnalysisAdd
BLAST
Topological domaini337 – 42690ExtracellularSequence AnalysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence AnalysisAdd
BLAST
Topological domaini448 – 4569CytoplasmicSequence Analysis

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chloride channel complex Source: UniProtKB-KW
  3. integral component of plasma membrane Source: InterPro
  4. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 456429Glycine receptor subunit alpha-4PRO_0000000422Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi171 ↔ 185By similarity
Disulfide bondi232 ↔ 243By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ61603.

PTM databases

PhosphoSiteiQ61603.

Expressioni

Gene expression databases

BgeeiQ61603.
CleanExiMM_GLRA4.
ExpressionAtlasiQ61603. baseline and differential.
GenevestigatoriQ61603.

Interactioni

Subunit structurei

Pentamer composed of alpha and beta subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ61603.
SMRiQ61603. Positions 47-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG265706.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ61603.
KOiK05271.
OMAiTKGSQPM.
OrthoDBiEOG712TVZ.
PhylomeDBiQ61603.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLVPASLF LLLWTLPGKV LLSVALAKED VKSGLKGSQP MSPSDFLDKL
60 70 80 90 100
MGRTSGYDAR IRPNFKGPPV NVTCNIFINS FGSVTETTMD YRVNVFLRQQ
110 120 130 140 150
WNDPRLAYRE YPDDSLDLDP SMLDSIWKPD LFFANEKGAN FHEVTTDNKL
160 170 180 190 200
LRIFKNGNVL YSIRLTLILS CPMDLKNFPM DIQTCTMQLE SFGYTMNDLM
210 220 230 240 250
FEWLEDAPAV QVAEGLTLPQ FILRDEKDLG YCTKHYNTGK FTCIEVKFHL
260 270 280 290 300
ERQMGYYLIQ MYIPSLLIVI LSWVSFWINM DAAPARVGLG ITTVLTMTTQ
310 320 330 340 350
SSGSRASLPK VSYVKAIDIW MAVCLLFVFA ALLEYAAVNF VSRQHKEFMR
360 370 380 390 400
LRRRQRRQRM EEDIIRESRF YFRGYGLGHC LQARDGGPME GSSIYSPQPP
410 420 430 440 450
TPLLKEGETM RKLYVDRAKR IDTISRAVFP FTFLVFNIFY WVVYKVLRSE

DIHQAL
Length:456
Mass (Da):52,514
Last modified:October 24, 2005 - v3
Checksum:i4199CB8404E05BEA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191D → N in AAL69899 (Ref. 4) Curated
Sequence conflicti124 – 1241D → E in AAL69899 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ109809 mRNA. Translation: AAZ17380.1.
AL671887 Genomic DNA. Translation: CAM17223.1.
X75850
, X75851, X75852, X75853 Genomic DNA. Translation: CAA53468.1.
AF462147 mRNA. Translation: AAL69899.1.
CCDSiCCDS53195.1.
PIRiA49970.
RefSeqiNP_034427.2. NM_010297.2.
XP_006528569.1. XM_006528506.2.
UniGeneiMm.215168.

Genome annotation databases

EnsembliENSMUST00000018739; ENSMUSP00000018739; ENSMUSG00000018595.
GeneIDi14657.
KEGGimmu:14657.
UCSCiuc009uiz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ109809 mRNA. Translation: AAZ17380.1.
AL671887 Genomic DNA. Translation: CAM17223.1.
X75850
, X75851, X75852, X75853 Genomic DNA. Translation: CAA53468.1.
AF462147 mRNA. Translation: AAL69899.1.
CCDSiCCDS53195.1.
PIRiA49970.
RefSeqiNP_034427.2. NM_010297.2.
XP_006528569.1. XM_006528506.2.
UniGeneiMm.215168.

3D structure databases

ProteinModelPortaliQ61603.
SMRiQ61603. Positions 47-451.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ61603.
ChEMBLiCHEMBL3500.
GuidetoPHARMACOLOGYi426.

PTM databases

PhosphoSiteiQ61603.

Proteomic databases

PRIDEiQ61603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018739; ENSMUSP00000018739; ENSMUSG00000018595.
GeneIDi14657.
KEGGimmu:14657.
UCSCiuc009uiz.2. mouse.

Organism-specific databases

CTDi441509.
MGIiMGI:95750. Glra4.

Phylogenomic databases

eggNOGiNOG265706.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ61603.
KOiK05271.
OMAiTKGSQPM.
OrthoDBiEOG712TVZ.
PhylomeDBiQ61603.
TreeFamiTF315453.

Miscellaneous databases

NextBioi286518.
PROiQ61603.
SOURCEiSearch...

Gene expression databases

BgeeiQ61603.
CleanExiMM_GLRA4.
ExpressionAtlasiQ61603. baseline and differential.
GenevestigatoriQ61603.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Glycine receptors containing the alpha4 subunit in the embryonic sympathetic nervous system, spinal cord and male genital ridge."
    Harvey R.J., Schmieden V., Von Holst A., Laube B., Rohrer H., Betz H.
    Eur. J. Neurosci. 12:994-1001(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Structural analysis of mouse glycine receptor alpha subunit genes. Identification and chromosomal localization of a novel variant."
    Matzenbach B., Maulet Y., Sefton L., Courtier B., Avner P., Guenet J.-L., Betz H.
    J. Biol. Chem. 269:2607-2612(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-360.
    Strain: BALB/c.
  4. "Localization of different glycine receptor isoforms in murine spinal cord."
    Groemer T.-W., Becker C.-M., Becker K.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 41-456.
    Strain: B6C3/Fe.
    Tissue: Spinal cord.

Entry informationi

Entry nameiGLRA4_MOUSE
AccessioniPrimary (citable) accession number: Q61603
Secondary accession number(s): A2AEA9, Q45V76, Q8VHF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: October 24, 2005
Last modified: March 31, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.