Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q61586

- GPAT1_MOUSE

UniProt

Q61586 - GPAT1_MOUSE

Protein

Glycerol-3-phosphate acyltransferase 1, mitochondrial

Gene

Gpam

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.By similarity

    Catalytic activityi

    Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. glycerol-3-phosphate O-acyltransferase activity Source: MGI

    GO - Biological processi

    1. acyl-CoA metabolic process Source: MGI
    2. CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
    3. defense response to virus Source: MGI
    4. fatty acid homeostasis Source: MGI
    5. fatty acid metabolic process Source: MGI
    6. glycerophospholipid metabolic process Source: MGI
    7. interleukin-2 secretion Source: MGI
    8. negative regulation of activation-induced cell death of T cells Source: MGI
    9. phospholipid homeostasis Source: MGI
    10. positive regulation of activated T cell proliferation Source: MGI
    11. positive regulation of multicellular organism growth Source: MGI
    12. regulation of cytokine secretion Source: MGI
    13. response to glucose Source: MGI
    14. triglyceride biosynthetic process Source: Ensembl
    15. triglyceride metabolic process Source: MGI

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    BRENDAi2.3.1.15. 3474.
    ReactomeiREACT_188640. Synthesis of PA.
    REACT_198969. Activation of gene expression by SREBF (SREBP).
    SABIO-RKQ61586.
    UniPathwayiUPA00557; UER00612.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate acyltransferase 1, mitochondrial (EC:2.3.1.15)
    Short name:
    GPAT-1
    Alternative name(s):
    P90
    Gene namesi
    Name:Gpam
    Synonyms:Gpat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:109162. Gpam.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: MGI
    3. mitochondrial outer membrane Source: UniProtKB-SubCell
    4. mitochondrion Source: MGI
    5. plasma membrane Source: InterPro

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 827Glycerol-3-phosphate acyltransferase 1, mitochondrialPRO_0000024691
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei380 – 3801PhosphoserineBy similarity
    Modified residuei687 – 6871Phosphoserine1 Publication
    Modified residuei694 – 6941Phosphoserine2 Publications
    Modified residuei779 – 7791N6-acetyllysine1 Publication
    Modified residuei783 – 7831N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ61586.
    PaxDbiQ61586.
    PRIDEiQ61586.

    PTM databases

    PhosphoSiteiQ61586.

    Expressioni

    Tissue specificityi

    Highest levels in liver, intermediate levels in muscle and kidney, and lowest levels in lung and brain.

    Gene expression databases

    ArrayExpressiQ61586.
    BgeeiQ61586.
    GenevestigatoriQ61586.

    Interactioni

    Protein-protein interaction databases

    BioGridi200011. 1 interaction.
    IntActiQ61586. 1 interaction.
    MINTiMINT-4119610.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61586.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini? – 471Mitochondrial intermembraneSequence Analysis
    Topological domaini495 – 57480CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini594 – 827234Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei472 – 49423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei575 – 59319HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi230 – 2356HXXXXD motif

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

    Sequence similaritiesi

    Belongs to the GPAT/DAPAT family.Curated

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2937.
    GeneTreeiENSGT00520000055570.
    HOVERGENiHBG000102.
    InParanoidiQ8VCT2.
    KOiK00629.
    OMAiKKNESLW.
    OrthoDBiEOG74R1PZ.
    TreeFamiTF313360.

    Family and domain databases

    InterProiIPR022284. GPAT/DHAPAT.
    IPR028354. GPAT_PlsB.
    IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view]
    PANTHERiPTHR12563. PTHR12563. 1 hit.
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500064. GPAT. 1 hit.
    PIRSF000437. GPAT_DHAPAT. 1 hit.
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61586-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEESSVTVGT IDVSYLPSSS EYSLGRCKHT SEDWVDCGFK PTFFRSATLK    50
    WKESLMSRKR PFVGRCCYSC TPQSWERFFN PSIPSLGLRN VIYINETHTR 100
    HRGWLARRLS YILFVQERDV HKGMFATSVT ENVLSSSRVQ EAIAEVAAEL 150
    NPDGSAQQQS KAIQKVKRKA RKILQEMVAT VSPGMIRLTG WVLLKLFNSF 200
    FWNIQIHKGQ LEMVKAATET NLPLLFLPVH RSHIDYLLLT FILFCHNIKA 250
    PYIASGNNLN IPVFSTLIHK LGGFFIRRRL DETPDGRKDI LYRALLHGHV 300
    VELLRQQQFL EIFLEGTRSR SGKTSCARAG LLSVVVDTLS SNTIPDILVI 350
    PVGISYDRII EGHYNGEQLG KPKKNESLWS VARGVIRMLR KNYGYVRVDF 400
    AQPFSLKEYL EGQSQKPVSA PLSLEQALLP AILPSRPNDV ADEHQDLSSN 450
    ESRNPADEAF RRRLIANLAE HILFTASKSC AIMSTHIVAC LLLYRHRQGI 500
    HLSTLVEDFF VMKEEVLARD FDLGFSGNSE DVVMHAIQLL GNCVTITHTS 550
    RKDEFFITPS TTVPSVFELN FYSNGVLHVF IMEAIIACSI YAVLNKRCSG 600
    GSAGGLGNLI SQEQLVRKAA SLCYLLSNEG TISLPCQTFY QVCHETVGKF 650
    IQYGILTVAE QDDQEDVSPG LAEQQWDKKL PELNWRSDEE DEDSDFGEEQ 700
    RDCYLKVSQS KEHQQFITFL QRLLGPLLEA YSSAAIFVHN FSGPVPESEY 750
    LQKLHRYLIT RTERNVAVYA ESATYCLVKN AVKMFKDIGV FKETKQKRVS 800
    VLELSSTFLP QCNRQKLLEY ILSFVVL 827
    Length:827
    Mass (Da):93,705
    Last modified:July 27, 2011 - v2
    Checksum:i4C177AA15374EE9B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti331 – 3311L → V in AAA37647. (PubMed:1721057)Curated
    Sequence conflicti337 – 3371D → N in AAA37647. (PubMed:1721057)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77003 mRNA. Translation: AAA37647.1.
    DQ479922 Genomic DNA. Translation: ABF48501.1.
    AK137067 mRNA. Translation: BAE23226.1.
    CH466585 Genomic DNA. Translation: EDL01718.1.
    CH466585 Genomic DNA. Translation: EDL01719.1.
    BC019201 mRNA. Translation: AAH19201.1.
    CCDSiCCDS29906.1.
    PIRiA41672.
    RefSeqiNP_032175.2. NM_008149.3.
    XP_006526755.1. XM_006526692.1.
    XP_006526756.1. XM_006526693.1.
    XP_006526757.1. XM_006526694.1.
    UniGeneiMm.210196.

    Genome annotation databases

    EnsembliENSMUST00000061856; ENSMUSP00000057635; ENSMUSG00000024978.
    GeneIDi14732.
    KEGGimmu:14732.
    UCSCiuc008hxk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77003 mRNA. Translation: AAA37647.1 .
    DQ479922 Genomic DNA. Translation: ABF48501.1 .
    AK137067 mRNA. Translation: BAE23226.1 .
    CH466585 Genomic DNA. Translation: EDL01718.1 .
    CH466585 Genomic DNA. Translation: EDL01719.1 .
    BC019201 mRNA. Translation: AAH19201.1 .
    CCDSi CCDS29906.1.
    PIRi A41672.
    RefSeqi NP_032175.2. NM_008149.3.
    XP_006526755.1. XM_006526692.1.
    XP_006526756.1. XM_006526693.1.
    XP_006526757.1. XM_006526694.1.
    UniGenei Mm.210196.

    3D structure databases

    ProteinModelPortali Q61586.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200011. 1 interaction.
    IntActi Q61586. 1 interaction.
    MINTi MINT-4119610.

    PTM databases

    PhosphoSitei Q61586.

    Proteomic databases

    MaxQBi Q61586.
    PaxDbi Q61586.
    PRIDEi Q61586.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000061856 ; ENSMUSP00000057635 ; ENSMUSG00000024978 .
    GeneIDi 14732.
    KEGGi mmu:14732.
    UCSCi uc008hxk.1. mouse.

    Organism-specific databases

    CTDi 57678.
    MGIi MGI:109162. Gpam.

    Phylogenomic databases

    eggNOGi COG2937.
    GeneTreei ENSGT00520000055570.
    HOVERGENi HBG000102.
    InParanoidi Q8VCT2.
    KOi K00629.
    OMAi KKNESLW.
    OrthoDBi EOG74R1PZ.
    TreeFami TF313360.

    Enzyme and pathway databases

    UniPathwayi UPA00557 ; UER00612 .
    BRENDAi 2.3.1.15. 3474.
    Reactomei REACT_188640. Synthesis of PA.
    REACT_198969. Activation of gene expression by SREBF (SREBP).
    SABIO-RK Q61586.

    Miscellaneous databases

    NextBioi 286773.
    PROi Q61586.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61586.
    Bgeei Q61586.
    Genevestigatori Q61586.

    Family and domain databases

    InterProi IPR022284. GPAT/DHAPAT.
    IPR028354. GPAT_PlsB.
    IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view ]
    PANTHERi PTHR12563. PTHR12563. 1 hit.
    Pfami PF01553. Acyltransferase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500064. GPAT. 1 hit.
    PIRSF000437. GPAT_DHAPAT. 1 hit.
    SMARTi SM00563. PlsC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcriptional regulation of p90 with sequence homology to Escherichia coli glycerol-3-phosphate acyltransferase."
      Shin D.-H., Paulauskis J.D., Moustaid N., Sul H.S.
      J. Biol. Chem. 266:23834-23839(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BTBR T+ tf/J.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-687 AND SER-694, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-694, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-779 AND LYS-783, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGPAT1_MOUSE
    AccessioniPrimary (citable) accession number: Q61586
    Secondary accession number(s): Q8VCT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3