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Q61584 (FXR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fragile X mental retardation syndrome-related protein 1

Short name=mFxr1p
Gene names
Name:Fxr1
Synonyms:Fxr1h
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length677 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. Ref.5

Subunit structure

Interacts with FMR1 and FXR2. Interacts with TDRD3 By similarity. Interacts with CYFIP2 but not with CYFIP1. Ref.4

Subcellular location

Cytoplasm By similarity.

Tissue specificity

In early embryogenesis, highest expression in somites and central nervous system. Also expressed in spinal cord, surrounding mesenchymal tissue and undifferentiated gonad. In mid-embryogenesis, most prominent in gonad and muscle tissue. Also expressed in liver, retina, telencephalon and mesencephalon. In late embryogenesis, restricted to skeletal muscle and proliferative active layers of brain. After birth, highly expressed in postmeiotic spermatids. Intermediate levels are found in heart, liver and kidney with lower levels in brain and skeletal muscle. Isoform(s) containing the 27 amino acid pocket (residues 564-590) are present in adult heart and muscle. Ref.2 Ref.6

Domain

The tandem Tudor domains preferentially recognize trimethylated histone peptides By similarity.

Disruption phenotype

Death shortly after birth. Mice expressing low levels of Fxr1 show postnatal growth retardation with reduced increase in muscle mass and strength. They die within 3 weeks of birth. Ref.5

Sequence similarities

Belongs to the FMR1 family.

Contains 2 Agenet-like domains.

Contains 2 KH domains.

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform E (identifier: Q61584-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: Q61584-2)

The sequence of this isoform differs from the canonical sequence as follows:
     380-408: Missing.
     564-568: DDSEK → GKRCD
     569-677: Missing.
Isoform B (identifier: Q61584-3)

The sequence of this isoform differs from the canonical sequence as follows:
     564-568: DDSEK → GKRCD
Isoform C (identifier: Q61584-4)

The sequence of this isoform differs from the canonical sequence as follows:
     564-590: Missing.
Isoform D (identifier: Q61584-5)

The sequence of this isoform differs from the canonical sequence as follows:
     380-408: Missing.
     564-590: Missing.
Isoform F (identifier: Q61584-6)

The sequence of this isoform differs from the canonical sequence as follows:
     380-408: Missing.
Isoform G (identifier: Q61584-7)

The sequence of this isoform differs from the canonical sequence as follows:
     430-455: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 677676Fragile X mental retardation syndrome-related protein 1
PRO_0000050107

Regions

Domain4 – 5047Agenet-like 1
Domain63 – 11553Agenet-like 2
Domain222 – 25130KH 1
Domain285 – 31430KH 2
Region471 – 48616RNA-binding RGG-box
Compositional bias50 – 534Poly-Pro
Compositional bias531 – 5399Poly-Arg

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue681Phosphotyrosine Ref.9
Modified residue4351Phosphoserine Ref.10
Modified residue4381Phosphoserine By similarity
Modified residue4491Phosphoserine Ref.10
Modified residue4521Phosphoserine Ref.10
Modified residue4741Omega-N-methylated arginine By similarity
Modified residue5141Phosphoserine By similarity
Modified residue6431Phosphoserine By similarity
Modified residue6671Phosphothreonine By similarity

Natural variations

Alternative sequence380 – 40829Missing in isoform A, isoform D and isoform F.
VSP_002836
Alternative sequence430 – 45526Missing in isoform G.
VSP_002837
Alternative sequence564 – 59027Missing in isoform C and isoform D.
VSP_002839
Alternative sequence564 – 5685DDSEK → GKRCD in isoform A and isoform B.
VSP_002838
Alternative sequence569 – 677109Missing in isoform A.
VSP_002840

Experimental info

Sequence conflict1361D → H in AAH19139. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform E [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: 908104FC95431A11

FASTA67776,222
        10         20         30         40         50         60 
MAELTVEVRG SNGAFYKGFI KDVHEDSLTV VFENNWQPER QVPFNEVRLP PPPDIKKEIS 

        70         80         90        100        110        120 
EGDEVEVYSR ANDQEPCGWW LAKVRMMKGE FYVIEYAACD ATYNEIVTFE RLRPVNQNKT 

       130        140        150        160        170        180 
VKKNTFFKCT VDVPEDLREA CANENAHKDF KKAVGACRIF YHPETTQLMI LSASEATVKR 

       190        200        210        220        230        240 
VNILSDMHLR SIRTKLMLMS RNEEATKHLE CTKQLAAAFH EEFVVREDLM GLAIGTHGSN 

       250        260        270        280        290        300 
IQQARKVPGV TAIELDEDTG TFRIYGESAE AVKKARGFLE FVEDFIQVPR NLVGKVIGKN 

       310        320        330        340        350        360 
GKVIQEIVDK SGVVRVRIEG DNENKLPRED GMVPFVFVGT KESIGNVQVL LEYHIAYLKE 

       370        380        390        400        410        420 
VEQLRMERLQ IDEQLRQIGM GFRPSSTRGP EREKGYATDE STVSSVQGSR SYSGRGRGRR 

       430        440        450        460        470        480 
GPNYTSGYGT NSELSNPSET ESERKDELSD WSLAGEDDRE TRHQRDSRRR PGGRGRSVSG 

       490        500        510        520        530        540 
GRGRGGPRGG KSSISSVLKD PDSNPYSLLD NTESDQTADT DASESHHSTN RRRRSRRRRT 

       550        560        570        580        590        600 
DEDAVLMDGL TESDTASVNE NGLDDSEKKP QRRNRSRRRR FRGQAEDRQP VTVADYISRA 

       610        620        630        640        650        660 
ESQSRQRNLP RETLAKNKKE MAKDVIEEHG PSEKAINGPT SASGDEIPKL PRTLGEEKTK 

       670 
TLKEDSTQEA AVLNGVS 

« Hide

Isoform A [UniParc].

Checksum: 46FC7A21E1C1697C
Show »

FASTA53960,816
Isoform B [UniParc].

Checksum: 189DF067FC611A20
Show »

FASTA67776,207
Isoform C [UniParc].

Checksum: 4E1E7192CF7A0E85
Show »

FASTA65072,814
Isoform D [UniParc].

Checksum: 92C675950981B238
Show »

FASTA62169,715
Isoform F [UniParc].

Checksum: EF2F32D4150CF24E
Show »

FASTA64873,123
Isoform G [UniParc].

Checksum: 9E24A06DDB03FA63
Show »

FASTA65173,358

References

« Hide 'large scale' references
[1]"Highly conserved 3' UTR and expression pattern of FXR1 points to a divergent gene regulation of FXR1 and FMR1."
Coy J.F., Sedlacek Z., Baechner D., Hameister H., Joos S., Lichter P., Delius H., Poustka A.
Hum. Mol. Genet. 4:2209-2218(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
Tissue: Fetal brain.
[2]"Alternative splicing in the murine and human FXR1 genes."
Kirkpatrick L.L., McIlwain K.A., Nelson D.L.
Genomics 59:193-202(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS A; B; C; D; E; F AND G), TISSUE SPECIFICITY.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Tissue: Kidney.
[4]"A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P."
Schenck A., Bardoni B., Moro A., Bagni C., Mandel J.-L.
Proc. Natl. Acad. Sci. U.S.A. 98:8844-8849(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CYFIP2.
[5]"Fxr1 knockout mice show a striated muscle phenotype: implications for Fxr1p function in vivo."
Mientjes E.J., Willemsen R., Kirkpatrick L.L., Nieuwenhuizen I.M., Hoogeveen-Westerveld M., Verweij M., Reis S., Bardoni B., Hoogeveen A.T., Oostra B.A., Nelson D.L.
Hum. Mol. Genet. 13:1291-1302(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"The RNA-binding protein Fragile X-related 1 regulates somite formation in Xenopus laevis."
Huot M.-E., Bisson N., Davidovic L., Mazroui R., Labelle Y., Moss T., Khandjian E.W.
Mol. Biol. Cell 16:4350-4361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[9]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[10]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-435; SER-449 AND SER-452, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X90875 mRNA. Translation: CAA62383.1.
AF124385 mRNA. Translation: AAD30211.1.
AF124394 expand/collapse EMBL AC list , AF124386, AF124387, AF124388, AF124389, AF124390, AF124391, AF124392, AF124393 Genomic DNA. Translation: AAD30212.1.
AF124394 expand/collapse EMBL AC list , AF124386, AF124387, AF124388, AF124389, AF124390, AF124391, AF124392, AF124393 Genomic DNA. Translation: AAD30213.1.
AF124394 expand/collapse EMBL AC list , AF124386, AF124387, AF124388, AF124389, AF124390, AF124391, AF124392, AF124393 Genomic DNA. Translation: AAD30214.1.
AF124394 expand/collapse EMBL AC list , AF124386, AF124387, AF124388, AF124389, AF124390, AF124391, AF124392, AF124393 Genomic DNA. Translation: AAD30215.1.
AF124394 expand/collapse EMBL AC list , AF124386, AF124387, AF124388, AF124389, AF124390, AF124391, AF124392, AF124393 Genomic DNA. Translation: AAD30216.1.
AF124394 expand/collapse EMBL AC list , AF124386, AF124387, AF124388, AF124389, AF124390, AF124391, AF124392, AF124393 Genomic DNA. Translation: AAD30217.1.
AF124394 expand/collapse EMBL AC list , AF124386, AF124387, AF124388, AF124389, AF124390, AF124391, AF124392, AF124393 Genomic DNA. Translation: AAD30218.1.
BC019139 mRNA. Translation: AAH19139.1.
CCDSCCDS50890.1. [Q61584-2]
CCDS50891.1. [Q61584-1]
RefSeqNP_001106659.1. NM_001113188.1. [Q61584-1]
NP_001106660.1. NM_001113189.1. [Q61584-5]
NP_032079.1. NM_008053.2. [Q61584-2]
XP_006535463.1. XM_006535400.1. [Q61584-4]
XP_006535464.1. XM_006535401.1. [Q61584-6]
XP_006535465.1. XM_006535402.1.
UniGeneMm.259021.

3D structure databases

ProteinModelPortalQ61584.
SMRQ61584. Positions 3-121, 212-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199772. 3 interactions.
IntActQ61584. 4 interactions.
MINTMINT-257148.

PTM databases

PhosphoSiteQ61584.

Proteomic databases

MaxQBQ61584.
PaxDbQ61584.
PRIDEQ61584.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001620; ENSMUSP00000001620; ENSMUSG00000027680. [Q61584-1]
ENSMUST00000167354; ENSMUSP00000130216; ENSMUSG00000027680. [Q61584-2]
GeneID14359.
KEGGmmu:14359.
UCSCuc008oxk.2. mouse. [Q61584-2]
uc008oxl.2. mouse. [Q61584-5]
uc008oxn.2. mouse. [Q61584-1]

Organism-specific databases

CTD8087.
MGIMGI:104860. Fxr1.

Phylogenomic databases

eggNOGNOG75351.
GeneTreeENSGT00390000017033.
HOVERGENHBG005739.
InParanoidQ61584.
KOK15516.
OMAGMVPFTF.
OrthoDBEOG7NKKJT.
PhylomeDBQ61584.
TreeFamTF105427.

Gene expression databases

ArrayExpressQ61584.
BgeeQ61584.
GenevestigatorQ61584.

Family and domain databases

Gene3D3.30.1370.10. 2 hits.
InterProIPR008395. Agenet-like_dom.
IPR022034. Frag_X_MRP_fam.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamPF05641. Agenet. 1 hit.
PF12235. FXR1P_C. 1 hit.
PF00013. KH_1. 2 hits.
[Graphical view]
SMARTSM00322. KH. 2 hits.
[Graphical view]
SUPFAMSSF54791. SSF54791. 2 hits.
PROSITEPS51641. AGENET_LIKE. 2 hits.
PS50084. KH_TYPE_1. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio285817.
PROQ61584.
SOURCESearch...

Entry information

Entry nameFXR1_MOUSE
AccessionPrimary (citable) accession number: Q61584
Secondary accession number(s): Q8VCU4 expand/collapse secondary AC list , Q9R1E2, Q9R1E3, Q9R1E4, Q9R1E5, Q9WUA7, Q9WUA8, Q9WUA9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot