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Q61581 (IBP7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor-binding protein 7

Short name=IBP-7
Short name=IGF-binding protein 7
Short name=IGFBP-7
Alternative name(s):
MAC25 protein
Gene names
Name:Igfbp7
Synonyms:Mac25
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds IGF-I and IGF-II with a relatively low affinity Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion By similarity.

Subcellular location

Secreted.

Tissue specificity

Expressed at high levels in lung, kidney, small intestine, testis and uterus and at moderate levels in liver. Ref.3

Induction

By retinoic acid.

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 IGFBP N-terminal domain.

Contains 1 Kazal-like domain.

RNA editing

Edited at positions 77 and 94.
Partially edited. Position 77 seems to be edited at about 56% and position 94 at about 58%. Ref.1 Ref.5

Sequence caution

The sequence BAB17228.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentSecreted
   Coding sequence diversityRNA editing
   DomainImmunoglobulin domain
Signal
   LigandGrowth factor binding
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

inner ear development

Inferred from direct assay PubMed 21795542. Source: MGI

regulation of cell growth

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 281256Insulin-like growth factor-binding protein 7
PRO_0000014393

Regions

Domain27 – 10377IGFBP N-terminal
Domain98 – 15760Kazal-like
Domain159 – 263105Ig-like C2-type

Amino acid modifications

Glycosylation1701N-linked (GlcNAc...) Potential
Disulfide bond180 ↔ 247 By similarity

Natural variations

Natural variant771R → G in RNA edited version.
Natural variant941K → R in RNA edited version.

Experimental info

Sequence conflict1461A → P in AAC37696. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61581 [UniParc].

Last modified September 19, 2002. Version 3.
Checksum: A70AE6B7D2D4AB58

FASTA28128,969
        10         20         30         40         50         60 
MERPPRALLL GAAGLLLLLL PLSSSSSSDA CGPCVPASCP ALPRLGCPLG ETRDACGCCP 

        70         80         90        100        110        120 
VCARGEGEPC GGGAAGRGHC APGMECVKSR KRRKGKAGAA AGGPATLAVC VCKSRYPVCG 

       130        140        150        160        170        180 
SNGITYPSGC QLRAASLRAE SRGEKAITQV SKGTCEQGPS IVTPPKDIWN VTGAKVFLSC 

       190        200        210        220        230        240 
EVIGIPTPVL IWNKVKRDHS GVQRTELLPG DRENLAIQTR GGPEKHEVTG WVLVSPLSKE 

       250        260        270        280 
DAGEYECHAS NSQGQASAAA KITVVDALHE IPLKKGEGAQ L 

« Hide

References

[1]"A follistatin-like gene, mac25, may act as a growth suppressor of osteosarcoma cells."
Kato M.V., Sato H., Tsukada T., Ikawa Y., Aizawa S., Nagayoshi M.
Oncogene 12:1361-1364(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING OF POSITION 94.
Tissue: Spleen.
[2]"A secreted tumor-suppressor, mac25, with activin-binding activity."
Kato M.V.
Mol. Med. 6:126-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Methylation and downregulated expression of mac25/insulin-like growth factor binding protein-7 is associated with liver tumorigenesis in SV40T/t antigen transgenic mice, screened by restriction landmark genomic scanning for methylation (RLGS-M)."
Komatsu S., Okazaki Y., Tateno M., Kawai J., Konno H., Kusakabe M., Yoshiki A., Muramatsu M., Held W.A., Hayashizaki Y.
Biochem. Biophys. Res. Commun. 267:109-117(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6.
[4]"Characterization of the promoter of the murine mac25 gene."
Kanemitsu N., Kato M.V., Miki T., Komatsu S., Okazaki Y., Hayashizaki Y., Sakai T.
Biochem. Biophys. Res. Commun. 279:251-257(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.
[5]"Screening of human SNP database identifies recoding sites of A-to-I RNA editing."
Gommans W.M., Tatalias N.E., Sie C.P., Dupuis D., Vendetti N., Smith L., Kaushal R., Maas S.
RNA 14:2074-2085(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA EDITING OF POSITIONS 77 AND 94.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L75822 mRNA. Translation: AAC37696.2.
AB012886 mRNA. Translation: BAA33569.1.
AB042198 Genomic DNA. Translation: BAB17228.1. Different initiation.
RefSeqNP_032074.3. NM_008048.3.
UniGeneMm.233470.

3D structure databases

ProteinModelPortalQ61581.
SMRQ61581. Positions 34-264.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid205897. 6 interactions.
IntActQ61581. 1 interaction.
MINTMINT-4098305.

PTM databases

PhosphoSiteQ61581.

Proteomic databases

MaxQBQ61581.
PaxDbQ61581.
PRIDEQ61581.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29817.
KEGGmmu:29817.

Organism-specific databases

CTD3490.
MGIMGI:1352480. Igfbp7.

Phylogenomic databases

eggNOGNOG40288.
HOVERGENHBG031621.
InParanoidQ61581.
PhylomeDBQ61581.

Gene expression databases

ArrayExpressQ61581.
BgeeQ61581.
CleanExMM_IGFBP7.
GenevestigatorQ61581.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR009030. Growth_fac_rcpt_N_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR000867. IGFBP-like.
IPR011390. IGFBP_rP_mac25.
IPR002350. Kazal_dom.
[Graphical view]
PANTHERPTHR14186. PTHR14186. 1 hit.
PfamPF07679. I-set. 1 hit.
PF00219. IGFBP. 1 hit.
PF07648. Kazal_2. 1 hit.
[Graphical view]
PIRSFPIRSF018239. IGFBP_rP_mac25. 1 hit.
SMARTSM00121. IB. 1 hit.
SM00409. IG. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIGFBP7. mouse.
NextBio306982.
PROQ61581.
SOURCESearch...

Entry information

Entry nameIBP7_MOUSE
AccessionPrimary (citable) accession number: Q61581
Secondary accession number(s): O88812, Q9EQW0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: September 19, 2002
Last modified: July 9, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot