Q61581 (IBP7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 7
Short name=IBP-7
Short name=IGF-binding protein 7
Short name=IGFBP-7

Alternative name(s):
MAC25 protein

Gene names

Name:	Igfbp7
Synonyms:	Mac25

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds IGF-I and IGF-II with a relatively low affinity Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed at high levels in lung, kidney, small intestine, testis and uterus and at moderate levels in liver. Ref.3
Induction	By retinoic acid.
Sequence similarities	Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 Kazal-like domain.
RNA editing	Edited at positions 77 and 94. Partially edited. Position 77 seems to be edited at about 56% and position 94 at about 58%. Ref.1 Ref.5
Sequence caution	The sequence BAB17228.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Secreted
Coding sequence diversity	RNA editing
Domain	Immunoglobulin domain Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB inner ear development Inferred from direct assay PubMed 21795542. Source: MGI regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 281	256	Insulin-like growth factor-binding protein 7		PRO_0000014393
Regions
	Domain	27 – 103	77	IGFBP N-terminal
	Domain	98 – 157	60	Kazal-like
	Domain	159 – 263	105	Ig-like C2-type
Amino acid modifications
	Glycosylation	170	1	N-linked (GlcNAc...) Potential
	Disulfide bond	180 ↔ 247		By similarity
Natural variations
	Natural variant	77	1	R → G in RNA edited version.
	Natural variant	94	1	K → R in RNA edited version.
Experimental info
	Sequence conflict	146	1	A → P in AAC37696. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q61581 [UniParc]. Last modified September 19, 2002. Version 3. Checksum: A70AE6B7D2D4AB58	FASTA	281	28,969
	10 20 30 40 50 60 MERPPRALLL GAAGLLLLLL PLSSSSSSDA CGPCVPASCP ALPRLGCPLG ETRDACGCCP 70 80 90 100 110 120 VCARGEGEPC GGGAAGRGHC APGMECVKSR KRRKGKAGAA AGGPATLAVC VCKSRYPVCG 130 140 150 160 170 180 SNGITYPSGC QLRAASLRAE SRGEKAITQV SKGTCEQGPS IVTPPKDIWN VTGAKVFLSC 190 200 210 220 230 240 EVIGIPTPVL IWNKVKRDHS GVQRTELLPG DRENLAIQTR GGPEKHEVTG WVLVSPLSKE 250 260 270 280 DAGEYECHAS NSQGQASAAA KITVVDALHE IPLKKGEGAQ L

References

[1]	"A follistatin-like gene, mac25, may act as a growth suppressor of osteosarcoma cells." Kato M.V., Sato H., Tsukada T., Ikawa Y., Aizawa S., Nagayoshi M. Oncogene 12:1361-1364(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING OF POSITION 94. Tissue: Spleen.
[2]	"A secreted tumor-suppressor, mac25, with activin-binding activity." Kato M.V. Mol. Med. 6:126-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Methylation and downregulated expression of mac25/insulin-like growth factor binding protein-7 is associated with liver tumorigenesis in SV40T/t antigen transgenic mice, screened by restriction landmark genomic scanning for methylation (RLGS-M)." Komatsu S., Okazaki Y., Tateno M., Kawai J., Konno H., Kusakabe M., Yoshiki A., Muramatsu M., Held W.A., Hayashizaki Y. Biochem. Biophys. Res. Commun. 267:109-117(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: C57BL/6.
[4]	"Characterization of the promoter of the murine mac25 gene." Kanemitsu N., Kato M.V., Miki T., Komatsu S., Okazaki Y., Hayashizaki Y., Sakai T. Biochem. Biophys. Res. Commun. 279:251-257(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.
[5]	"Screening of human SNP database identifies recoding sites of A-to-I RNA editing." Gommans W.M., Tatalias N.E., Sie C.P., Dupuis D., Vendetti N., Smith L., Kaushal R., Maas S. RNA 14:2074-2085(2008) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITIONS 77 AND 94.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L75822 mRNA. Translation: AAC37696.2. AB012886 mRNA. Translation: BAA33569.1. AB042198 Genomic DNA. Translation: BAB17228.1. Different initiation.
RefSeq	NP_032074.3. NM_008048.3.
UniGene	Mm.233470.
3D structure databases
ProteinModelPortal	Q61581.
SMR	Q61581. Positions 34-264.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	205897. 6 interactions.
IntAct	Q61581. 1 interaction.
MINT	MINT-4098305.
PTM databases
PhosphoSite	Q61581.
Proteomic databases
MaxQB	Q61581.
PaxDb	Q61581.
PRIDE	Q61581.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29817.
KEGG	mmu:29817.
Organism-specific databases
CTD	3490.
MGI	MGI:1352480. Igfbp7.
Phylogenomic databases
eggNOG	NOG40288.
HOVERGEN	HBG031621.
InParanoid	Q61581.
PhylomeDB	Q61581.
Gene expression databases
ArrayExpress	Q61581.
Bgee	Q61581.
CleanEx	MM_IGFBP7.
Genevestigator	Q61581.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt_N_dom. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR000867. IGFBP-like. IPR011390. IGFBP_rP_mac25. IPR002350. Kazal_dom. [Graphical view]
PANTHER	PTHR14186. PTHR14186. 1 hit.
Pfam	PF07679. I-set. 1 hit. PF00219. IGFBP. 1 hit. PF07648. Kazal_2. 1 hit. [Graphical view]
PIRSF	PIRSF018239. IGFBP_rP_mac25. 1 hit.
SMART	SM00121. IB. 1 hit. SM00409. IG. 1 hit. SM00280. KAZAL. 1 hit. [Graphical view]
SUPFAM	SSF57184. SSF57184. 1 hit.
PROSITE	PS50835. IG_LIKE. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS51465. KAZAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	IGFBP7. mouse.
NextBio	306982.
PRO	Q61581.
SOURCE	Search...

Entry information

Entry name

IBP7_MOUSE

Accession

Primary (citable) accession number: Q61581
Secondary accession number(s): O88812, Q9EQW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	September 19, 2002
Last modified:	July 9, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program