Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADPH:adrenodoxin oxidoreductase, mitochondrial

Gene

Fdxr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.

Catalytic activityi

2 reduced adrenodoxin + NADP+ = 2 oxidized adrenodoxin + NADPH.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511FAD; via amide nitrogenBy similarity
Binding sitei72 – 721FADBy similarity
Binding sitei80 – 801FAD; via amide nitrogenBy similarity
Binding sitei116 – 1161FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei243 – 2431NADPBy similarity
Binding sitei401 – 4011FAD; via amide nitrogenBy similarity
Binding sitei408 – 4081NADP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi187 – 1904NADPBy similarity
Nucleotide bindingi231 – 2322NADPBy similarity
Nucleotide bindingi408 – 4103FADBy similarity

GO - Molecular functioni

  1. NADPH-adrenodoxin reductase activity Source: GO_Central
  2. NADPH binding Source: Ensembl

GO - Biological processi

  1. cholesterol metabolic process Source: UniProtKB-UniPathway
  2. NADPH oxidation Source: Ensembl
  3. ubiquinone biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Electron transport, Lipid metabolism, Steroid metabolism, Sterol metabolism, Transport

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiREACT_281686. Endogenous sterols.
REACT_294407. Pregnenolone biosynthesis.
REACT_304388. Electron transport from NADPH to Ferredoxin.
REACT_332536. Defective CYP11A1 causes Adrenal insufficiency, congenital, with 46,XY sex reversal (AICSR).
UniPathwayiUPA00296.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH:adrenodoxin oxidoreductase, mitochondrial (EC:1.18.1.6)
Short name:
AR
Short name:
Adrenodoxin reductase
Alternative name(s):
Ferredoxin--NADP(+) reductase
Short name:
Ferredoxin reductase
Gene namesi
Name:Fdxr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104724. Fdxr.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: MGI
  2. mitochondrial matrix Source: UniProtKB-SubCell
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionSequence AnalysisAdd
BLAST
Chaini35 – 494460NADPH:adrenodoxin oxidoreductase, mitochondrialPRO_0000019421Add
BLAST

Proteomic databases

MaxQBiQ61578.
PaxDbiQ61578.
PRIDEiQ61578.

2D gel databases

REPRODUCTION-2DPAGEQ61578.

PTM databases

PhosphoSiteiQ61578.

Expressioni

Tissue specificityi

Expressed in the adrenal, testis and ovary and to a lesser extent in the liver and kidney.

Gene expression databases

BgeeiQ61578.
CleanExiMM_FDXR.
ExpressionAtlasiQ61578. baseline and differential.
GenevestigatoriQ61578.

Interactioni

Subunit structurei

Monomer. Interacts directly with FDX1.By similarity

Protein-protein interaction databases

IntActiQ61578. 1 interaction.
MINTiMINT-151907.

Structurei

3D structure databases

SMRiQ61578. Positions 40-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0493.
GeneTreeiENSGT00390000013574.
HOVERGENiHBG002132.
InParanoidiQ61578.
KOiK18914.
OMAiCHTSTPG.
OrthoDBiEOG7H1JJX.
TreeFamiTF314193.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR021163. Ferredox_Rdtase_adrenod.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000362. FNR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRCWHWWR WSAWSGLRPS PSRSTPTPGF CQKFSTQEKT PQICVVGSGP
60 70 80 90 100
AGFYTAQHLL KHHTHAHVDI YEKQLVPFGL VRFGVAPDHP EVKNVINTFT
110 120 130 140 150
QTARSDRCAF QGNVVVGRDV SVPELREAYH AVVLSYGAED HQPLGIPGEE
160 170 180 190 200
LPGVVSARAF VGWYNGLPEN QELAPDLSCD TAVILGQGNV ALDVARILLT
210 220 230 240 250
PPEHLEKTDI TEAALGALRQ SRVKTVWIVG RRGPLQVAFT IKELREMIQL
260 270 280 290 300
PGTRPILDPS DFLGLQDRIK DVPRPRRRLT ELLLRTATEK PGVEEAARQA
310 320 330 340 350
LASRAWGLRF FRSPQQVLPT PDGQRVAGIR LAVTSLEGVG ESTRAVPTGD
360 370 380 390 400
VEDLPCGLLL SSVGYKSRPI DPSVPFDPKL GVIPNTEGRV VNVPGLYCSG
410 420 430 440 450
WVKRGPTGVI TTTMTDSFLT SQALLEDLKA GLLPSGPRPG YVAIQALLSN
460 470 480 490
RGVRPVSFSD WEKLDAEEVS RGQGTGKPRE KLVDRREMLR LLGH
Length:494
Mass (Da):54,202
Last modified:November 1, 1996 - v1
Checksum:i4BD279DFC606A5C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49920 mRNA. Translation: BAA08659.1.
CCDSiCCDS25625.1.
PIRiS60028.
RefSeqiNP_032023.1. NM_007997.1.
UniGeneiMm.4719.

Genome annotation databases

EnsembliENSMUST00000021078; ENSMUSP00000021078; ENSMUSG00000018861.
GeneIDi14149.
KEGGimmu:14149.
UCSCiuc007mgy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49920 mRNA. Translation: BAA08659.1.
CCDSiCCDS25625.1.
PIRiS60028.
RefSeqiNP_032023.1. NM_007997.1.
UniGeneiMm.4719.

3D structure databases

SMRiQ61578. Positions 40-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61578. 1 interaction.
MINTiMINT-151907.

PTM databases

PhosphoSiteiQ61578.

2D gel databases

REPRODUCTION-2DPAGEQ61578.

Proteomic databases

MaxQBiQ61578.
PaxDbiQ61578.
PRIDEiQ61578.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021078; ENSMUSP00000021078; ENSMUSG00000018861.
GeneIDi14149.
KEGGimmu:14149.
UCSCiuc007mgy.1. mouse.

Organism-specific databases

CTDi2232.
MGIiMGI:104724. Fdxr.

Phylogenomic databases

eggNOGiCOG0493.
GeneTreeiENSGT00390000013574.
HOVERGENiHBG002132.
InParanoidiQ61578.
KOiK18914.
OMAiCHTSTPG.
OrthoDBiEOG7H1JJX.
TreeFamiTF314193.

Enzyme and pathway databases

UniPathwayiUPA00296.
ReactomeiREACT_281686. Endogenous sterols.
REACT_294407. Pregnenolone biosynthesis.
REACT_304388. Electron transport from NADPH to Ferredoxin.
REACT_332536. Defective CYP11A1 causes Adrenal insufficiency, congenital, with 46,XY sex reversal (AICSR).

Miscellaneous databases

NextBioi285258.
PROiQ61578.
SOURCEiSearch...

Gene expression databases

BgeeiQ61578.
CleanExiMM_FDXR.
ExpressionAtlasiQ61578. baseline and differential.
GenevestigatoriQ61578.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR021163. Ferredox_Rdtase_adrenod.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000362. FNR. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning of mouse ferredoxin reductase from kidney."
    Itoh S., Iemura O., Yamada E., Yoshimura T., Tsujikawa K., Kohama Y., Mimura T.
    Biochim. Biophys. Acta 1264:159-162(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.

Entry informationi

Entry nameiADRO_MOUSE
AccessioniPrimary (citable) accession number: Q61578
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.