Q61553 (FSCN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fascin

Alternative name(s):
Singed-like protein

Gene names

Name:	Fscn1
Synonyms:	Fan1, Snl

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration By similarity.
Subunit structure	Associates with beta-catenin.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cell projection › filopodium By similarity. Cell projection › invadopodium By similarity.
Tissue specificity	Most abundant in brain. Found in other tissues including uterus, small intestine and spleen.
Domain	Composed of four beta-trefoil domains By similarity.
Post-translational modification	Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin By similarity.
Sequence similarities	Belongs to the fascin family.

Ontologies

Keywords
Cellular component	Cell junction Cell projection Cytoplasm Cytoskeleton
Ligand	Actin-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	actin filament bundle assembly Inferred from direct assay Ref.1. Source: MGI cell migration Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW filopodium Inferred from direct assay. Source: MGI invadopodium Inferred from sequence or structural similarity. Source: UniProtKB stress fiber Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	actin filament binding Inferred from mutant phenotype. Source: MGI drug binding Inferred from direct assay Ref.6. Source: UniProtKB protein binding, bridging Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 493	492	Fascin		PRO_0000219380
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity
	Modified residue	38	1	Phosphoserine By similarity
	Modified residue	39	1	Phosphoserine; by PKC By similarity
	Modified residue	471	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	64	1	S → T in AAB41126. Ref.1
	Sequence conflict	64	1	S → T in AAB58784. Ref.2
	Sequence conflict	262	1	Q → E in AAB41126. Ref.1
	Sequence conflict	262	1	Q → E in AAB58784. Ref.2
	Sequence conflict	271	1	R → G in AAB41126. Ref.1
	Sequence conflict	271	1	R → G in AAB58784. Ref.2
	Sequence conflict	362 – 363	2	QL → HV in AAB41126. Ref.1
	Sequence conflict	362 – 363	2	QL → HV in AAB58784. Ref.2
	Sequence conflict	387	1	V → A in AAB41126. Ref.1
	Sequence conflict	387	1	V → A in AAB58784. Ref.2
	Sequence conflict	396	1	G → A in AAB41126. Ref.1
	Sequence conflict	396	1	G → A in AAB58784. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61553 [UniParc]. Last modified May 1, 2007. Version 4. Checksum: 1C6DCD5211B74969	FASTA	493	54,508
	10 20 30 40 50 60 MTANGTAEAV QIQFGLISCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV 70 80 90 100 110 120 CLRSHLGRYL AADKDGNVTC EREVPDGDCR FLVVAHDDGR WSLQSEAHRR YFGGTEDRLS 130 140 150 160 170 180 CFAQSVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA 190 200 210 220 230 240 FQDQRYSVQT SDHRFLRHDG RLVARPEPAT GFTLEFRSGK VAFRDCEGRY LAPSGPSGTL 250 260 270 280 290 300 KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR 310 320 330 340 350 360 DTRKCAFRTH TGKYWTLTAT GGVQSTASTK NASCYFDIEW CDRRITLRAS NGKFVTAKKN 370 380 390 400 410 420 GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND 430 440 450 460 470 480 GAYNIKDSTG KYWTVGSDSS VTSSSDTPVD FFLEFCDYNK VALKVGGRYL KGDHAGVLKA 490 CAETIDPASL WEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a murine fascin homolog from mouse brain." Edwards R.A., Herrera-Sosa H., Otto J., Bryan J. J. Biol. Chem. 270:10764-10770(1995) [PubMed: 7738015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11. Strain: BALB/c. Tissue: Brain.
[2]	"Beta-catenin associates with the actin-bundling protein fascin in a noncadherin complex." Tao Y.S., Edwards R.A., Tubb B., Wang S., Bryan J., McCrea P.D. J. Cell Biol. 134:1271-1281(1996) [PubMed: 8794867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Placenta.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Embryonic brain and Mammary gland.
[5]	Lubec G., Klug S., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-32; 69-82; 132-149; 168-185; 202-217; 248-271; 380-389 AND 409-426, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain and Hippocampus.
[6]	"Migrastatin analogues target fascin to block tumour metastasis." Chen L., Yang S., Jakoncic J., Zhang J.J., Huang X.Y. Nature 464:1062-1066(2010) [PubMed: 20393565] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33726 mRNA. Translation: AAB41126.1. U90355 Genomic DNA. Translation: AAB58784.1. AK167670 mRNA. Translation: BAE39719.1. BC010338 mRNA. Translation: AAH10338.1. BC037137 mRNA. Translation: AAH37137.2. BC052408 mRNA. Translation: AAH52408.1.
IPI	IPI00353563.
PIR	A56430.
RefSeq	NP_032010.2. NM_007984.2.
UniGene	Mm.289707.
3D structure databases
ProteinModelPortal	Q61553.
SMR	Q61553. Positions 8-493.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61553. 1 interaction.
STRING	Q61553.
PTM databases
PhosphoSite	Q61553.
2D gel databases
REPRODUCTION-2DPAGE	IPI00353563.
Proteomic databases
PRIDE	Q61553.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031565; ENSMUSP00000031565; ENSMUSG00000029581.
GeneID	14086.
KEGG	mmu:14086.
UCSC	uc009ajl.1. mouse.
Organism-specific databases
CTD	6624.
MGI	MGI:1352745. Fscn1.
Phylogenomic databases
eggNOG	roNOG15184.
GeneTree	ENSGT00530000063373.
HOGENOM	HBG446238.
HOVERGEN	HBG000968.
InParanoid	Q61553.
OMA	VNIYSLT.
OrthoDB	EOG41NTKS.
PhylomeDB	Q61553.
Gene expression databases
ArrayExpress	Q61553.
Bgee	Q61553.
Genevestigator	Q61553.
GermOnline	ENSMUSG00000029581. Mus musculus.
Family and domain databases
InterPro	IPR008999. Actin_cross-linking. IPR024703. Fascin. IPR022768. Fascin-domain. [Graphical view]
Pfam	PF06268. Fascin. 4 hits. [Graphical view]
PIRSF	PIRSF005682. Fascin. 1 hit.
SUPFAM	SSF50405. Actin_crosslink. 4 hits.
ProtoNet	Search...
Other
NextBio	285112.
SOURCE	Search...

Entry information

Entry name

FSCN1_MOUSE

Accession

Primary (citable) accession number: Q61553
Secondary accession number(s): O09099 , O09156, Q05DK3, Q7TN32, Q80V75

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 2007
Last modified:	November 16, 2011
This is version 99 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program