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Protein

Double-strand-break repair protein rad21 homolog

Gene

Rad21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. Plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway (By similarity). The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.By similarity

Miscellaneous

Seems to bind calcium.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis, Cell cycle, Cell division, Chromosome partition, DNA damage, DNA repair, Mitosis
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-2467813 Separation of Sister Chromatids
R-MMU-2468052 Establishment of Sister Chromatid Cohesion
R-MMU-2470946 Cohesin Loading onto Chromatin
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand-break repair protein rad21 homolog
Alternative name(s):
Pokeweed agglutinin-binding protein 29
Short name:
PW29
SCC1 homolog
Gene namesi
Name:Rad21
Synonyms:Hr21, Scc1By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:108016 Rad21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000978731 – 635Double-strand-break repair protein rad21 homologAdd BLAST635

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei46PhosphoserineBy similarity1
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei153PhosphoserineCombined sources1
Modified residuei175PhosphoserineCombined sources1
Cross-linki216Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei249PhosphoserineBy similarity1
Modified residuei394PhosphothreonineBy similarity1
Cross-linki418Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei458PhosphoserineBy similarity1
Modified residuei549PhosphoserineBy similarity1
Modified residuei627PhosphothreonineBy similarity1

Post-translational modificationi

Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites (By similarity).By similarity
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei172 – 173Cleavage; by ESPL1By similarity2
Sitei279 – 280Cleavage; by caspase-3 or caspase-7By similarity2
Sitei454 – 455Cleavage; by ESPL1By similarity2

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61550
MaxQBiQ61550
PaxDbiQ61550
PeptideAtlasiQ61550
PRIDEiQ61550

PTM databases

iPTMnetiQ61550
PhosphoSitePlusiQ61550

Expressioni

Tissue specificityi

Widely expressed with highest levels in testis, brain, kidney, heart and thymus. Lowest levels in skeletal muscle.1 Publication

Developmental stagei

Abundance does not change during cell cycle.

Gene expression databases

BgeeiENSMUSG00000022314
CleanExiMM_RAD21
GenevisibleiQ61550 MM

Interactioni

Subunit structurei

Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Found in a complex with SMC1A, SMC3, CDCA5, PDS5A/SCC-112 and PDS5B/APRIN. Interacts with PDS5B and WAPL; the interaction is direct. Interacts with DDX11. Found in a cohesin complex with SMC1A, SMC3 and STAG1 (By similarity). Interacts with SMC1A and SMC3 (PubMed:10375619).By similarity1 Publication

Protein-protein interaction databases

BioGridi202560, 23 interactors
CORUMiQ61550
DIPiDIP-56655N
IntActiQ61550, 19 interactors
MINTiQ61550
STRINGi10090.ENSMUSP00000022927

Structurei

3D structure databases

ProteinModelPortaliQ61550
SMRiQ61550
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni287 – 403Interaction with WAPL and PDS5BBy similarityAdd BLAST117
Regioni362 – 403Interaction with STAG1By similarityAdd BLAST42

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi473 – 514Pro-richAdd BLAST42
Compositional biasi499 – 557Glu-richAdd BLAST59

Domaini

The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.By similarity

Sequence similaritiesi

Belongs to the rad21 family.Curated

Phylogenomic databases

eggNOGiKOG1213 Eukaryota
ENOG410XRB4 LUCA
GeneTreeiENSGT00390000011606
HOGENOMiHOG000233800
HOVERGENiHBG059956
InParanoidiQ61550
KOiK06670
OMAiKRLMHWK
OrthoDBiEOG091G03QW
TreeFamiTF101215

Family and domain databases

InterProiView protein in InterPro
IPR006909 Rad21/Rec8_C_eu
IPR006910 Rad21_Rec8_N
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF04824 Rad21_Rec8, 1 hit
PF04825 Rad21_Rec8_N, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

Q61550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK
60 70 80 90 100
MALRTSGHLL LGVVRIYHRK AKYLLADCNE AFIKIKMAFR PGVVDLPEEN
110 120 130 140 150
REAAYNAITL PEEFHDFDQP LPDLDDIDVA QQFSLNQSRV EEITMREEVG
160 170 180 190 200
NISILQENDF GDFGMDDREI MREGSAFEDD DMLVSTSASN LLLEPEQSTS
210 220 230 240 250
NLNEKMNHLE YEDQYKDDNF GEGNDGGILD DKLISNNDGG IFDDPPALSE
260 270 280 290 300
AGVMLPEQPA HDDMDEDDNG SLGGPDSPDS VDPVEPMPTM TDQTTLVPNE
310 320 330 340 350
EEAFALEPID ITVKETKAKR KRKLIVDSVK ELDSKTIRAQ LSDYSDIVTT
360 370 380 390 400
LDLAPPTKKL MMWKETGGVE KLFFLPAQPL WNNRLLKLFT RCLTPLVPED
410 420 430 440 450
LRKRRKGGEA DNLDEFLKEF ENPEVPREEQ QPQQQQPQPQ RDVIDEPIIE
460 470 480 490 500
EPSRLQDSVM EASRTTIEES AMPPPPPQGV KRKAGQIDPE PSIPPQQVEQ
510 520 530 540 550
MEIPPVELPP EEPPNICQLI PELELLPEKE KEKEKEKEEE EEEEDEDASG
560 570 580 590 600
GDQDQEERRW NKRTQQMLHG LQRALAKTGA ESISLLELCR NTNRKQAAAK
610 620 630
FYSFLVLKKQ QAIELTQEEP YSDIIATPGP RFHII
Length:635
Mass (Da):72,083
Last modified:July 27, 2011 - v3
Checksum:i2EC1AC08E358E9CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147E → G in BAB23527 (PubMed:16141072).Curated1
Sequence conflicti205K → E in BAB23527 (PubMed:16141072).Curated1
Sequence conflicti374F → S in BAA08408 (PubMed:8521526).Curated1
Sequence conflicti374F → S in AAK56113 (PubMed:11471062).Curated1
Sequence conflicti374F → S in AAK56114 (PubMed:11471062).Curated1
Sequence conflicti544Missing in BAA08408 (PubMed:8521526).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49429 mRNA Translation: BAA08408.1
X98293 mRNA Translation: CAA66939.1
AF332086 mRNA Translation: AAK56114.1
AF332085 mRNA Translation: AAK56113.1
AK004746 mRNA Translation: BAB23527.1
AK163992 mRNA Translation: BAE37576.1
BC043032 mRNA Translation: AAH43032.2
CCDSiCCDS27463.1
PIRiJC4248
RefSeqiNP_033035.3, NM_009009.4
XP_006520707.1, XM_006520644.1
XP_006520708.1, XM_006520645.1
UniGeneiMm.182628

Genome annotation databases

EnsembliENSMUST00000022927; ENSMUSP00000022927; ENSMUSG00000022314
GeneIDi19357
KEGGimmu:19357
UCSCiuc007vrd.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRAD21_MOUSE
AccessioniPrimary (citable) accession number: Q61550
Secondary accession number(s): P70219
, Q3TQ09, Q810A8, Q91VB9, Q9DBU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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