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Protein

Adhesion G protein-coupled receptor E1

Gene

Adgre1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Adaptive immunity, Immunity

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiS63.951.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor E1
Alternative name(s):
Cell surface glycoprotein F4/80
EGF-like module receptor 1
EGF-like module-containing mucin-like hormone receptor-like 1
EMR1 hormone receptor
Gene namesi
Name:Adgre1
Synonyms:Emr1, Gpf480
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:106912. Adgre1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 644617ExtracellularCuratedAdd
BLAST
Transmembranei645 – 67228Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini673 – 6797CytoplasmicCurated
Transmembranei680 – 70122Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini702 – 71110ExtracellularCurated
Transmembranei712 – 73524Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini736 – 75419CytoplasmicCuratedAdd
BLAST
Transmembranei755 – 77622Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini777 – 79216ExtracellularCuratedAdd
BLAST
Transmembranei793 – 82129Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini822 – 83918CytoplasmicCuratedAdd
BLAST
Transmembranei840 – 85920Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini860 – 87415ExtracellularCuratedAdd
BLAST
Transmembranei875 – 89723Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini898 – 93134CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • cell periphery Source: MGI
  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice fail to develop peripheral tolerance after inoculation with antigen because of a lack of efferent regulatory T cell development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 931904Adhesion G protein-coupled receptor E1PRO_0000012874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 48PROSITE-ProRule annotation
Disulfide bondi42 ↔ 57PROSITE-ProRule annotation
Disulfide bondi59 ↔ 79PROSITE-ProRule annotation
Disulfide bondi85 ↔ 98PROSITE-ProRule annotation
Disulfide bondi92 ↔ 107PROSITE-ProRule annotation
Disulfide bondi109 ↔ 131PROSITE-ProRule annotation
Disulfide bondi137 ↔ 149PROSITE-ProRule annotation
Disulfide bondi143 ↔ 158PROSITE-ProRule annotation
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi160 ↔ 171PROSITE-ProRule annotation
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi177 ↔ 189PROSITE-ProRule annotation
Disulfide bondi183 ↔ 198PROSITE-ProRule annotation
Disulfide bondi200 ↔ 220PROSITE-ProRule annotation
Disulfide bondi226 ↔ 239PROSITE-ProRule annotation
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi233 ↔ 248PROSITE-ProRule annotation
Disulfide bondi250 ↔ 270PROSITE-ProRule annotation
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi276 ↔ 286PROSITE-ProRule annotation
Disulfide bondi280 ↔ 295PROSITE-ProRule annotation
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi297 ↔ 317PROSITE-ProRule annotation
Disulfide bondi323 ↔ 336PROSITE-ProRule annotation
Disulfide bondi330 ↔ 345PROSITE-ProRule annotation
Disulfide bondi347 ↔ 366PROSITE-ProRule annotation
Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi474 – 4741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi498 – 4981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi706 – 7061N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61549.
PaxDbiQ61549.
PRIDEiQ61549.

PTM databases

PhosphoSiteiQ61549.

Expressioni

Tissue specificityi

In macrophages; but absent from those which are localized within T-cell areas of lymph nodes and spleen. Low level of expression on blood monocytes.

Gene expression databases

BgeeiQ61549.
ExpressionAtlasiQ61549. baseline and differential.
GenevisibleiQ61549. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004850.

Structurei

3D structure databases

ProteinModelPortaliQ61549.
SMRiQ61549. Positions 42-355, 436-629, 649-893.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 8049EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini81 – 13252EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 17240EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini173 – 22149EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini222 – 27150EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini272 – 31847EGF-like 6; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini319 – 36749EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini592 – 64150GPSPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi506 – 5083Cell attachment siteSequence Analysis

Sequence similaritiesi

Contains 7 EGF-like domains.PROSITE-ProRule annotation
Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239437.
GeneTreeiENSGT00760000118787.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ61549.
KOiK04591.
OMAiRNHNTYL.
OrthoDBiEOG7WHH8Q.
PhylomeDBiQ61549.
TreeFamiTF316380.

Family and domain databases

InterProiIPR026823. cEGF.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR009030. Growth_fac_rcpt_N_dom.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF12662. cEGF. 2 hits.
PF07645. EGF_CA. 3 hits.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00181. EGF. 1 hit.
SM00179. EGF_CA. 6 hits.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 6 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 7 hits.
PS01187. EGF_CA. 5 hits.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGFWLLLFW GFSGMYRWGM TTLPTLGQTL GGVNECQDTT TCPAYATCTD
60 70 80 90 100
TTDSYYCTCK RGFLSSNGQT NFQGPGVECQ DVNECLQSDS PCGPNSVCTN
110 120 130 140 150
ILGRAKCSCL RGFSSSTGKD WILGSLDNFL CADVDECLTI GICPKYSNCS
160 170 180 190 200
NSVGSYSCTC QPGFVLNGSI CEDEDECVTR DVCPEHATCH NTLGSYYCTC
210 220 230 240 250
NSGLESSGGG PMFQGLDESC EDVDECSRNS TLCGPTFICI NTLGSYSCSC
260 270 280 290 300
PAGFSLPTFQ ILGHPADGNC TDIDECDDTC PLNSSCTNTI GSYFCTCHPG
310 320 330 340 350
FASSNGQLNF KDLEVTCEDI DECTQDPLQC GLNSVCTNVP GSYICGCLPD
360 370 380 390 400
FQMDPEGSQG YGNFNCKRIL FKCKEDLILQ SEQIQQCQAV QGRDLGYASF
410 420 430 440 450
CTLVNATFTI LDNTCENKSA PVSLQSAATS VSLVLEQATT WFELSKEETS
460 470 480 490 500
TLGTILLETV ESTMLAALLI PSGNASQMIQ TEYLDIESKV INEECKENES
510 520 530 540 550
INLAARGDKM NVGCFIIKES VSTGAPGVAF VSFAHMESVL NERFFEDGQS
560 570 580 590 600
FRKLRMNSRV VGGTVTGEKK EDFSKPIIYT LQHIQPKQKS ERPICVSWNT
610 620 630 640 650
DVEDGRWTPS GCEIVEASET HTVCSCNRMA NLAIIMASGE LTMEFSLYII
660 670 680 690 700
SHVGTVISLV CLALAIATFL LCRAVQNHNT YMHLHLCVCL FLAKILFLTG
710 720 730 740 750
IDKTDNQTAC AIIAGFLHYL FLACFFWMLV EAVMLFLMVR NLKVVNYFSS
760 770 780 790 800
RNIKMLHLCA FGYGLPVLVV IISASVQPRG YGMHNRCWLN TETGFIWSFL
810 820 830 840 850
GPVCMIITIN SVLLAWTLWV LRQKLCSVSS EVSKLKDTRL LTFKAIAQIF
860 870 880 890 900
ILGCSWVLGI FQIGPLASIM AYLFTIINSL QGAFIFLIHC LLNRQVRDEY
910 920 930
KKLLTRKTDL SSHSQTSGIL LSSMPSTSKM G
Length:931
Mass (Da):102,130
Last modified:November 1, 1997 - v1
Checksum:i52963A667E8B76B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93328 mRNA. Translation: CAA63720.1.
U66888 mRNA. Translation: AAC53184.1.
BC075688 mRNA. Translation: AAH75688.1.
CCDSiCCDS50160.1.
RefSeqiNP_034260.1. NM_010130.4.
XP_006523664.1. XM_006523601.1.
UniGeneiMm.2254.

Genome annotation databases

EnsembliENSMUST00000004850; ENSMUSP00000004850; ENSMUSG00000004730.
ENSMUST00000086763; ENSMUSP00000083971; ENSMUSG00000004730.
GeneIDi13733.
KEGGimmu:13733.
UCSCiuc008det.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93328 mRNA. Translation: CAA63720.1.
U66888 mRNA. Translation: AAC53184.1.
BC075688 mRNA. Translation: AAH75688.1.
CCDSiCCDS50160.1.
RefSeqiNP_034260.1. NM_010130.4.
XP_006523664.1. XM_006523601.1.
UniGeneiMm.2254.

3D structure databases

ProteinModelPortaliQ61549.
SMRiQ61549. Positions 42-355, 436-629, 649-893.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004850.

Protein family/group databases

MEROPSiS63.951.
GPCRDBiSearch...

PTM databases

PhosphoSiteiQ61549.

Proteomic databases

MaxQBiQ61549.
PaxDbiQ61549.
PRIDEiQ61549.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004850; ENSMUSP00000004850; ENSMUSG00000004730.
ENSMUST00000086763; ENSMUSP00000083971; ENSMUSG00000004730.
GeneIDi13733.
KEGGimmu:13733.
UCSCiuc008det.2. mouse.

Organism-specific databases

CTDi2015.
MGIiMGI:106912. Adgre1.

Phylogenomic databases

eggNOGiNOG239437.
GeneTreeiENSGT00760000118787.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ61549.
KOiK04591.
OMAiRNHNTYL.
OrthoDBiEOG7WHH8Q.
PhylomeDBiQ61549.
TreeFamiTF316380.

Miscellaneous databases

NextBioi284538.
PROiQ61549.
SOURCEiSearch...

Gene expression databases

BgeeiQ61549.
ExpressionAtlasiQ61549. baseline and differential.
GenevisibleiQ61549. MM.

Family and domain databases

InterProiIPR026823. cEGF.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR009030. Growth_fac_rcpt_N_dom.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF12662. cEGF. 2 hits.
PF07645. EGF_CA. 3 hits.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00181. EGF. 1 hit.
SM00179. EGF_CA. 6 hits.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 6 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 7 hits.
PS01187. EGF_CA. 5 hits.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of F4/80, a murine macrophage-restricted cell surface glycoprotein with homology to the G-protein-linked transmembrane 7 hormone receptor family."
    McKnight A.J., Macfarlane A.J., Dri P., Turley L., Willis A.C., Gordon S.
    J. Biol. Chem. 271:486-489(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Peritoneal cavity.
  2. "Identification and characterization of a seven transmembrane hormone receptor using differential display."
    Lin H.H., Stubbs L.J., Mucenski M.L.
    Genomics 41:301-308(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  4. "The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance."
    Lin H.H., Faunce D.E., Stacey M., Terajewicz A., Nakamura T., Zhang-Hoover J., Kerley M., Mucenski M.L., Gordon S., Stein-Streilein J.
    J. Exp. Med. 201:1615-1625(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiAGRE1_MOUSE
AccessioniPrimary (citable) accession number: Q61549
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Most adhesion GPCRs proteins undergo autoproteolysis at the GPS domain. ADGRE1 is predicted non-cleavable because of the lack of a consensus catalytic triad sequence within GPS domain.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.