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Protein

Clathrin coat assembly protein AP180

Gene

Snap91

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: BHF-UCL
  • clathrin heavy chain binding Source: BHF-UCL
  • protein kinase binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • clathrin coat assembly Source: BHF-UCL
  • neurotransmitter secretion Source: BHF-UCL
  • protein transport Source: UniProtKB-KW
  • regulation of clathrin-mediated endocytosis Source: MGI
  • synaptic transmission Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin coat assembly protein AP180
Alternative name(s):
91 kDa synaptosomal-associated protein
Clathrin coat-associated protein AP180
Phosphoprotein F1-20
Gene namesi
Name:Snap91
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109132. Snap91.

Subcellular locationi

GO - Cellular componenti

  • AP-2 adaptor complex Source: BHF-UCL
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 901901Clathrin coat assembly protein AP180PRO_0000193865Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei296 – 2961PhosphoserineBy similarity
Modified residuei300 – 3001PhosphoserineBy similarity
Modified residuei306 – 3061PhosphoserineBy similarity
Glycosylationi310 – 3101O-linked (GlcNAc)By similarity
Modified residuei313 – 3131PhosphoserineCombined sources
Modified residuei317 – 3171PhosphothreonineCombined sources
Modified residuei594 – 5941PhosphoserineBy similarity
Modified residuei600 – 6001PhosphoserineBy similarity
Modified residuei621 – 6211PhosphoserineBy similarity
Modified residuei627 – 6271PhosphoserineBy similarity
Modified residuei761 – 7611PhosphoserineBy similarity

Post-translational modificationi

Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ61548.
MaxQBiQ61548.
PaxDbiQ61548.
PRIDEiQ61548.

PTM databases

iPTMnetiQ61548.
PhosphoSiteiQ61548.
SwissPalmiQ61548.

Expressioni

Tissue specificityi

Brain. Associated with the synapses.

Developmental stagei

Developmentally regulated in a pattern coincident with active synaptogenesis and synaptic maturation.

Gene expression databases

CleanExiMM_SNAP91.

Interactioni

Subunit structurei

Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity).By similarity

GO - Molecular functioni

  • clathrin heavy chain binding Source: BHF-UCL
  • protein kinase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

IntActiQ61548. 3 interactions.
MINTiMINT-1636263.
STRINGi10090.ENSMUSP00000074066.

Structurei

3D structure databases

ProteinModelPortaliQ61548.
SMRiQ61548. Positions 18-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 145132ENTHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi410 – 4134Poly-Thr
Compositional biasi535 – 5395Poly-Ala
Compositional biasi547 – 5504Poly-Ala
Compositional biasi659 – 6646Poly-Ser
Compositional biasi704 – 7107Poly-Ser

Domaini

Possesses a three domain structure: the N-terminal 300 residues harbor a clathrin binding site, an acidic middle domain 450 residues, interrupted by an Ala-rich segment, and the C-terminal domain (166 residues).

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0251. Eukaryota.
ENOG410XQ90. LUCA.
HOGENOMiHOG000015763.
HOVERGENiHBG049391.
InParanoidiQ61548.
PhylomeDBiQ61548.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR030428. AP180_vert.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR22951:SF4. PTHR22951:SF4. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q61548-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN
60 70 80 90 100
ETNVNIPQMA DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGSHGYDM STFIRRYSRY LNEKAFSYRQ MAFDFARVKK
160 170 180 190 200
GADGVMRTMV PEKLLKSMPI LQGQIDALLE FDVHPNELTN GVINAAFMLL
210 220 230 240 250
FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF LTRMTRVSEF
260 270 280 290 300
LKVAEQVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS
310 320 330 340 350
PLSKSSPATT VTSPNSTPAK TIDTSPPVDI FATASAAAPV SSAKPSSDLL
360 370 380 390 400
DLQPDFSGAA AGAAAPVVPP SGGATAWGDL LGEDSLAALS SVPCEAPISD
410 420 430 440 450
PFAPEPSPPT TTTEPASASA STTTAVTAVT TEVDLFGDAF AASPGEAPAA
460 470 480 490 500
SEGATAPATP APVAAALDAC SGNDPFAPSE GSAEAAPELD LFAMKPPETS
510 520 530 540 550
APVVTPTAST APPVPATAPS PAPTAVAATA ATTTAAAAAT TTATTSAAAA
560 570 580 590 600
TTAAAPPALD IFGDLFDSAP EVAAAPKPDA APSIDLFGTD AFSSPPRGAS
610 620 630 640 650
PVPESSLTAD LLSVDAFAAP SPASTASPAK AESSGVIDLF GDAFGSGASE
660 670 680 690 700
TQPAPQAVSS SSASADLLAG FGGSFMAPST TPVTPAQNNL LQPSFEAAFG
710 720 730 740 750
TTPSTSSSSS FDPSVFDGLG DLLMPTMAPS GQPAPVSMVP PSPAMAASKG
760 770 780 790 800
LGSDLDSSLA SLVGNLGISG TTSKKGDLQW NAGEKKLTGG ANWQPKVTPA
810 820 830 840 850
TWSAGVPPQG TVPPTSSVPP GAGAPSVGQP GAGFGMPPSG TGMTMMSQQP
860 870 880 890 900
VMFAQPMMRP PFGAAAVPGT QLSPSPTPAT QSPKKPPAKD PLADLNIKDF

L
Length:901
Mass (Da):91,851
Last modified:November 1, 1996 - v1
Checksum:i24A98FBACE8DB8B1
GO
Isoform Short (identifier: Q61548-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     715-719: Missing.

Show »
Length:896
Mass (Da):91,320
Checksum:i39A36ADC3B905055
GO
Isoform 3 (identifier: Q61548-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     715-719: Missing.
     809-836: Missing.

Note: No experimental confirmation available.
Show »
Length:868
Mass (Da):88,829
Checksum:i0DED8D018E5D7D9C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei715 – 7195Missing in isoform Short and isoform 3. 2 PublicationsVSP_000172
Alternative sequencei809 – 83628Missing in isoform 3. 1 PublicationVSP_022635Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83985 mRNA. Translation: AAA37587.1.
M83985 mRNA. Translation: AAA37586.1.
BC031773 mRNA. Translation: AAH31773.1.
PIRiA44825.
RefSeqiNP_001264911.1. NM_001277982.1. [Q61548-3]
NP_001264915.1. NM_001277986.1. [Q61548-2]
NP_038697.1. NM_013669.2. [Q61548-1]
XP_006510957.1. XM_006510894.2. [Q61548-1]
XP_006510963.1. XM_006510900.2. [Q61548-3]
UniGeneiMm.281651.
Mm.472993.

Genome annotation databases

GeneIDi20616.
KEGGimmu:20616.
UCSCiuc029xff.2. mouse. [Q61548-3]
uc033jlv.1. mouse. [Q61548-2]
uc033jlw.1. mouse. [Q61548-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83985 mRNA. Translation: AAA37587.1.
M83985 mRNA. Translation: AAA37586.1.
BC031773 mRNA. Translation: AAH31773.1.
PIRiA44825.
RefSeqiNP_001264911.1. NM_001277982.1. [Q61548-3]
NP_001264915.1. NM_001277986.1. [Q61548-2]
NP_038697.1. NM_013669.2. [Q61548-1]
XP_006510957.1. XM_006510894.2. [Q61548-1]
XP_006510963.1. XM_006510900.2. [Q61548-3]
UniGeneiMm.281651.
Mm.472993.

3D structure databases

ProteinModelPortaliQ61548.
SMRiQ61548. Positions 18-264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61548. 3 interactions.
MINTiMINT-1636263.
STRINGi10090.ENSMUSP00000074066.

PTM databases

iPTMnetiQ61548.
PhosphoSiteiQ61548.
SwissPalmiQ61548.

Proteomic databases

EPDiQ61548.
MaxQBiQ61548.
PaxDbiQ61548.
PRIDEiQ61548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20616.
KEGGimmu:20616.
UCSCiuc029xff.2. mouse. [Q61548-3]
uc033jlv.1. mouse. [Q61548-2]
uc033jlw.1. mouse. [Q61548-1]

Organism-specific databases

CTDi9892.
MGIiMGI:109132. Snap91.

Phylogenomic databases

eggNOGiKOG0251. Eukaryota.
ENOG410XQ90. LUCA.
HOGENOMiHOG000015763.
HOVERGENiHBG049391.
InParanoidiQ61548.
PhylomeDBiQ61548.

Miscellaneous databases

ChiTaRSiSnap91. mouse.
NextBioi298991.
PROiQ61548.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SNAP91.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR030428. AP180_vert.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR22951:SF4. PTHR22951:SF4. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel synapse-specific protein. II. cDNA cloning and sequence analysis of the F1-20 protein."
    Zhou S., Sousa R., Tannery N.H., Lafer E.M.
    J. Neurosci. 12:2144-2155(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Eye.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313 AND THR-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue and Heart.

Entry informationi

Entry nameiAP180_MOUSE
AccessioniPrimary (citable) accession number: Q61548
Secondary accession number(s): Q61547, Q8K0D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.