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Protein

Golgi apparatus protein 1

Gene

Glg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds fibroblast growth factor (By similarity). Binds E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils).By similarity

GO - Biological processi

  • bone morphogenesis Source: MGI
  • negative regulation of protein processing Source: MGI
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • regulation of chondrocyte differentiation Source: MGI
Complete GO annotation...

Keywords - Ligandi

Sialic acid

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi apparatus protein 1
Alternative name(s):
E-selectin ligand 1
Short name:
ESL-1
Short name:
Selel
Golgi sialoglycoprotein MG-160
Gene namesi
Name:Glg1
Synonyms:Esl1, Mg160, Selel
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:104967. Glg1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 11411114ExtracellularSequence analysisAdd
BLAST
Transmembranei1142 – 116221HelicalSequence analysisAdd
BLAST
Topological domaini1163 – 117513CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 11751148Golgi apparatus protein 1PRO_0000011121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi161 – 1611N-linked (GlcNAc...)1 Publication
Glycosylationi206 – 2061N-linked (GlcNAc...)1 Publication
Glycosylationi577 – 5771N-linked (GlcNAc...)Sequence analysis
Glycosylationi673 – 6731N-linked (GlcNAc...)1 Publication
Glycosylationi782 – 7821N-linked (GlcNAc...)Sequence analysis
Modified residuei957 – 9571PhosphoserineCombined sources

Post-translational modificationi

Fucosylation is essential for binding to E-selectin.
Contains sialic acid residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ61543.
MaxQBiQ61543.
PaxDbiQ61543.
PRIDEiQ61543.

PTM databases

iPTMnetiQ61543.
PhosphoSiteiQ61543.
SwissPalmiQ61543.

Expressioni

Tissue specificityi

Widely expressed; found in myeloid cells, fibroblasts, colon carcinoma, endothelioma, teratocarcinoma, lymphoma, myeloma.

Gene expression databases

BgeeiQ61543.
CleanExiMM_GLG1.
ExpressionAtlasiQ61543. baseline and differential.
GenevisibleiQ61543. MM.

Interactioni

Protein-protein interaction databases

DIPiDIP-59329N.
IntActiQ61543. 3 interactions.
MINTiMINT-1836383.
STRINGi10090.ENSMUSP00000131355.

Structurei

3D structure databases

ProteinModelPortaliQ61543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati112 – 14534Cys-rich GLG1 1Add
BLAST
Repeati146 – 20863Cys-rich GLG1 2Add
BLAST
Repeati211 – 27464Cys-rich GLG1 3Add
BLAST
Repeati282 – 34261Cys-rich GLG1 4Add
BLAST
Repeati343 – 40967Cys-rich GLG1 5Add
BLAST
Repeati410 – 46960Cys-rich GLG1 6Add
BLAST
Repeati471 – 53363Cys-rich GLG1 7Add
BLAST
Repeati534 – 60067Cys-rich GLG1 8Add
BLAST
Repeati605 – 66460Cys-rich GLG1 9Add
BLAST
Repeati666 – 72459Cys-rich GLG1 10Add
BLAST
Repeati725 – 78460Cys-rich GLG1 11Add
BLAST
Repeati792 – 85261Cys-rich GLG1 12Add
BLAST
Repeati854 – 90754Cys-rich GLG1 13Add
BLAST
Repeati908 – 97568Cys-rich GLG1 14Add
BLAST
Repeati976 – 103156Cys-rich GLG1 15Add
BLAST
Repeati1037 – 109761Cys-rich GLG1 16Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 516Poly-Gly
Compositional biasi66 – 705Poly-Gln
Compositional biasi74 – 829Poly-Gln

Sequence similaritiesi

Contains 16 Cys-rich GLG1 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3648. Eukaryota.
ENOG410XNWV. LUCA.
GeneTreeiENSGT00390000011262.
HOGENOMiHOG000047635.
HOVERGENiHBG051850.
InParanoidiQ61543.
KOiK06816.
OMAiNCQQALQ.
OrthoDBiEOG7XH6P1.
PhylomeDBiQ61543.
TreeFamiTF106112.

Family and domain databases

InterProiIPR001893. Cys-rich_GLG1_repeat.
IPR017873. Cys-rich_GLG1_repeat_euk.
[Graphical view]
PfamiPF00839. Cys_rich_FGFR. 15 hits.
[Graphical view]
PROSITEiPS51289. GLG1_C_RICH. 16 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVCGRVRGM FRLSAALPLL LLAAAGAQNG HGQGQGPGTN FGPFPGQGGG
60 70 80 90 100
GSPAGQQPPQ QPQLSQQQQQ PPPQQQQQQQ QQSLFAAGGL PARRGGAGPG
110 120 130 140 150
GTGGGWKLAE EESCREDVTR VCPKHTWSNN LAVLECLQDV REPENEISSD
160 170 180 190 200
CNHLLWNYKL NLTTDPKFES VAREVCKSTI SEIKECAEEP VGKGYMVSCL
210 220 230 240 250
VDHRGNITEY QCHQYITKMT AIIFSDYRLI CGFMDDCKND INLLKCGSIR
260 270 280 290 300
LGEKDAHSQG EVVSCLEKGL VKEAEEKEPK IQVSELCKKA ILRVAELSSD
310 320 330 340 350
DFHLDRHLYF ACRDDRERFC ENTQAGEGRV YKCLFNHKFE ESMSEKCREA
360 370 380 390 400
LTTRQKLIAQ DYKVSYSLAK SCKSDLKKYR CNVENLPRSR EARLSYLLMC
410 420 430 440 450
LESAVHRGRQ VSSECQGEML DYRRMLMEDF SLSPEIILSC RGEIEHHCSG
460 470 480 490 500
LHRKGRTLHC LMKVVRGEKG NLGMNCQQAL QTLIQETDPG ADYRIDRALN
510 520 530 540 550
EACESVIQTA CKHIRSGDPM ILSCLMEHLY TEKMVEDCEH RLLELQYFIS
560 570 580 590 600
RDWKLDPVLY RKCQGDASRL CHTHGWNETS ELMPPGAVFS CLYRHAYRTE
610 620 630 640 650
EQGRRLSREC RAEVQRILHQ RAMDVKLDPA LQDKCLIDLG KWCSEKTETG
660 670 680 690 700
QELECLQDHL DDLAVECRDI VGNLTELESE DIQIEALLMR ACEPIIQNFC
710 720 730 740 750
HDVADNQIDS GDLMECLIQN KHQKDMNEKC AIGVTHFQLV QMKDFRFSYK
760 770 780 790 800
FKMACKEDVL KLCPNIKKKV DVVICLSTTV RNDTLQEAKE HRVSLKCRKQ
810 820 830 840 850
LRVEELEMTE DIRLEPDLYE ACKSDIKNYC STVQYGNAQI IECLKENKKQ
860 870 880 890 900
LSTRCHQKVF KLQETEMMDP ELDYTLMRVC KQMIKRFCPE ADSKTMLQCL
910 920 930 940 950
KQNKNSELMD PKCKQMITKR QITQNTDYRL NPVLRKACKA DIPKFCHGIL
960 970 980 990 1000
TKAKDDSELE GQVISCLKLR YADQRLSSDC EDQIRIIIQE SALDYRLDPQ
1010 1020 1030 1040 1050
LQLHCSDEIA NLCAEEAAAQ EQTGQVEECL KVNLLKIKTE LCKKEVLNML
1060 1070 1080 1090 1100
KESKADIFVD PVLHTACALD IKHHCAAITP GRGRQMSCLM EALEDKRVRL
1110 1120 1130 1140 1150
QPECKKRLND RIEMWSYAAK VAPADGFSDL AMQVMTSPSK NYILSVISGS
1160 1170
ICILFLIGLM CGRITKRVTR ELKDR
Length:1,175
Mass (Da):133,734
Last modified:November 1, 1996 - v1
Checksum:i105835DD38C7338B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84037 mRNA. Translation: CAA58855.1.
BC021306 mRNA. Translation: AAH21306.1.
Y12462 Genomic DNA. Translation: CAA73066.1.
CCDSiCCDS22671.1.
PIRiS52417.
RefSeqiNP_033175.1. NM_009149.2.
UniGeneiMm.276271.
Mm.440619.

Genome annotation databases

EnsembliENSMUST00000169020; ENSMUSP00000131355; ENSMUSG00000003316.
GeneIDi20340.
KEGGimmu:20340.
UCSCiuc009nma.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84037 mRNA. Translation: CAA58855.1.
BC021306 mRNA. Translation: AAH21306.1.
Y12462 Genomic DNA. Translation: CAA73066.1.
CCDSiCCDS22671.1.
PIRiS52417.
RefSeqiNP_033175.1. NM_009149.2.
UniGeneiMm.276271.
Mm.440619.

3D structure databases

ProteinModelPortaliQ61543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59329N.
IntActiQ61543. 3 interactions.
MINTiMINT-1836383.
STRINGi10090.ENSMUSP00000131355.

PTM databases

iPTMnetiQ61543.
PhosphoSiteiQ61543.
SwissPalmiQ61543.

Proteomic databases

EPDiQ61543.
MaxQBiQ61543.
PaxDbiQ61543.
PRIDEiQ61543.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000169020; ENSMUSP00000131355; ENSMUSG00000003316.
GeneIDi20340.
KEGGimmu:20340.
UCSCiuc009nma.1. mouse.

Organism-specific databases

CTDi2734.
MGIiMGI:104967. Glg1.

Phylogenomic databases

eggNOGiKOG3648. Eukaryota.
ENOG410XNWV. LUCA.
GeneTreeiENSGT00390000011262.
HOGENOMiHOG000047635.
HOVERGENiHBG051850.
InParanoidiQ61543.
KOiK06816.
OMAiNCQQALQ.
OrthoDBiEOG7XH6P1.
PhylomeDBiQ61543.
TreeFamiTF106112.

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.

Miscellaneous databases

ChiTaRSiGlg1. mouse.
PROiQ61543.
SOURCEiSearch...

Gene expression databases

BgeeiQ61543.
CleanExiMM_GLG1.
ExpressionAtlasiQ61543. baseline and differential.
GenevisibleiQ61543. MM.

Family and domain databases

InterProiIPR001893. Cys-rich_GLG1_repeat.
IPR017873. Cys-rich_GLG1_repeat_euk.
[Graphical view]
PfamiPF00839. Cys_rich_FGFR. 15 hits.
[Graphical view]
PROSITEiPS51289. GLG1_C_RICH. 16 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The E-selectin-ligand ESL-1 is a variant of a receptor for fibroblast growth factor."
    Steegmaier M., Levinovitz A., Isenmann S., Borges E., Lenter M., Kocher H.P., Kleuser B., Vestweber D.
    Nature 373:615-620(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 255-266; 357-363; 636-641 AND 744-750.
    Tissue: Neutrophil.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  3. "Structure of the murine E-selectin ligand 1 (ESL-1) gene and assignment to chromosome 8."
    Willmroth F., Beaudet A.L.
    Mamm. Genome 10:1085-1088(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-138.
    Strain: 129/Sv.
    Tissue: Embryonic stem cell.
  4. "The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface."
    Steegmaier M., Borges E., Berger J., Schwarz H., Vestweber D.
    J. Cell Sci. 110:687-694(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-161 AND ASN-673.
    Tissue: Myoblast.
  6. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-957, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiGSLG1_MOUSE
AccessioniPrimary (citable) accession number: Q61543
Secondary accession number(s): Q9QZ40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.