Mitogen-activated protein kinase 6 - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot Q61532 (MK06_MOUSE)

Last modified November 3, 2009. Version 86. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase 6
    EC=2.7.11.24
Alternative name(s):
    Extracellular signal-regulated kinase 3
      Short name=ERK-3

Gene names

Name:	Mapk6
Synonyms:	Erk3, Prkm4, Prkm6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity.
Developmental stage	Expression increases markedly from days 9 to 11 in the developing embryo, followed by a gradual decrease up to birth. Ref.1
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-625 and Tyr-627, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

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Ontologies

Keywords
Biological process	Cell cycle
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 720

720

Mitogen-activated protein kinase 6

PRO_0000186258

Regions

Domain

20 – 316

297

Protein kinase

Nucleotide binding

26 – 34

ATP By similarity

Motif

625 – 627

TXY

Sites

Active site

152

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

189

Phosphoserine Ref.5

Modified residue

386

Phosphoserine By similarity

Modified residue

389

Phosphothreonine By similarity

Modified residue

625

Phosphothreonine By similarity

Modified residue

627

Phosphotyrosine By similarity

Experimental info

Sequence conflict

R → T in AAN64588. Ref.2

Sequence conflict

40 – 41

ND → KY in AAN64588. Ref.2

Sequence conflict

L → P in AAI00386. Ref.3

Sequence conflict

123

P → S in AAF61348. Ref.1

Sequence conflict

574

E → K in AAN64588. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Q61532-1 [UniParc].

Last modified September 13, 2004. Version 3.
Checksum: D8BC667DEF6F62E2
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FASTA

720

82,199

        10         20         30         40         50         60 
MAEKFESLMN IHGFDLGSRY MDLKPLGCGG NGLVFSAVDN DCDKRVAIKK IVLTDPQSVK 

        70         80         90        100        110        120 
HALREIKIIR RLDHDNIVKV FEILGPSGSQ LTDDVGSLTE LNSVYIVQEY METDLANVLE 

       130        140        150        160        170        180 
QGPLLEEHAR LFMYQLLRGL KYIHSANVLH RDLKPANLFI NTEDLVLKIG DFGLARIMDP 

       190        200        210        220        230        240 
HYSHKGHLSE GLVTKWYRSP RLLLSPNNYT KAIDMWAAGC IFAEMLTGKT LFAGAHELEQ 

       250        260        270        280        290        300 
MQLILDSIPV VHEEDRQELL SVIPVYIRND MTEPHRPLTQ LLPGISREAL DFLEQILTFS 

       310        320        330        340        350        360 
PMDRLTAEEA LSHPYMSIYS FPTDEPISSH PFHIEDEVDD ILLMDETHSH IYNWERYHDC 

       370        380        390        400        410        420 
QFSEHDWPIH NNFDIDEVQL DPRALSDVTD EEEVQVDPRK YLDGDREKYL EDPAFDTSYS 

       430        440        450        460        470        480 
AEPCWQYPDH HENKYCDLEC SHTCNYKTRS SPYLDNLVWR ESEVNHYYEP KLIIDLSNWK 

       490        500        510        520        530        540 
EQSKEKSDKR GKSKCERNGL VKAQIALEEA SQQLAERERG QGFDFDSFIA GTIQLSAQHQ 

       550        560        570        580        590        600 
SADVVDKLND LNSSVSQLEL KSLISKSVSR EKQEKGRANL AQLGALYQSS WDSQFVSGGE 

       610        620        630        640        650        660 
ECFLISQFCC EVRKDEHAEK ENTYTSYLDK FFSRKEDSEM LETEPVEEGK RGERGREAGL 

       670        680        690        700        710        720 
LSGGGEFLLS KQLESIGTPQ FHSPVGSPLK SIQATLTPSA MKSSPQIPHK TYSSILKHLN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of mouse extracellular-signal-regulated protein kinase 3 as a unique gene product of 100 kDa." Turgeon B., Saba-El-Leil M.K., Meloche S. Biochem. J. 346:169-175(2000) [PubMed: 10657254] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Tissue: Pituitary.
[2]	"The protein kinase ERK3 is encoded by a single functional gene: genomic analysis of the ERK3 gene family." Turgeon B., Lang B.F., Meloche S. Genomics 80:673-680(2002) [PubMed: 12504858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and Czech II. Tissue: Embryonic brain, Embryonic germ cell, Mammary tumor and Olfactory epithelium.
[4]	"Novel CDC2-related protein kinases produced in murine hematopoietic stem cells." Ershler M.A., Nagorskaya T.V., Visser J.W.M., Belyavsky A.V. Gene 124:305-306(1993) [PubMed: 8444355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-195. Strain: CBA. Tissue: Hematopoietic.
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF132850 mRNA. Translation: AAF61348.1. AY134883 , AY134660, AY134880, AY134881, AY134882 Genomic DNA. Translation: AAN64588.1. BC006778 mRNA. Translation: AAH06778.1. BC048779 mRNA. Translation: AAH48779.1. BC052420 mRNA. Translation: AAH52420.2. BC100385 mRNA. Translation: AAI00386.1. X64607 mRNA. Translation: CAA45891.1.
IPI	IPI00122366.
PIR	PN0481.
RefSeq	NP_056621.4. NP_081694.1.
UniGene	Mm.18856
3D structure databases
HSSP	HSSP built from PDB template 1KV1 based on UniProtKB Q16539.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61532.
PTM databases
PhosphoSite	Q61532.
Proteomic databases
PRIDE	Q61532.
Genome annotation databases
Ensembl	ENSMUST00000049355; ENSMUSP00000040315; ENSMUSG00000042688; Mus musculus. [Genome view]
GeneID	50772.
KEGG	mmu:50772.
NMPDR	fig\|10090.3.peg.20505.
UCSC	uc009qsd.1. mouse.
Organism-specific databases
CTD	50772.
MGI	MGI:1354946. Mapk6.
Phylogenomic databases
HOVERGEN	Q61532.
OMA	PAFDTSY.
Enzyme and pathway databases
BRENDA	2.7.11.24. 244.
Gene expression databases
ArrayExpress	Q61532.
Bgee	Q61532.
CleanEx	MM_MAPK6.
Genevestigator	Q61532.
GermOnline	ENSMUSG00000042688. Mus musculus.
Family and domain databases
InterPro	IPR008350. Erk_3_4_MAPK. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01771. ERK3ERK4MAPK.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	307695.
SOURCE	Search...

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Entry information

Entry name

MK06_MOUSE

Accession

Primary (citable) accession number: Q61532
Secondary accession number(s): Q497T9

Q9JLU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 13, 2004
Last modified:	November 3, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents