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Q61508

- ECM1_MOUSE

UniProt

Q61508 - ECM1_MOUSE

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Protein

Extracellular matrix protein 1

Gene

Ecm1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).By similarity

GO - Molecular functioni

  1. interleukin-2 receptor binding Source: MGI
  2. signal transducer activity Source: Ensembl

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. biomineral tissue development Source: UniProtKB-KW
  3. inflammatory response Source: MGI
  4. negative regulation of bone mineralization Source: UniProtKB
  5. negative regulation of cytokine-mediated signaling pathway Source: MGI
  6. negative regulation of peptidase activity Source: UniProtKB
  7. ossification Source: UniProtKB-KW
  8. positive regulation of angiogenesis Source: UniProtKB
  9. positive regulation of endothelial cell proliferation Source: UniProtKB
  10. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  11. regulation of T cell migration Source: MGI
  12. regulation of transcription from RNA polymerase II promoter Source: MGI
  13. regulation of type 2 immune response Source: MGI
Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Biomineralization, Mineral balance, Osteogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular matrix protein 1
Alternative name(s):
Secretory component p85
Gene namesi
Name:Ecm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:103060. Ecm1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
  2. extracellular vesicular exosome Source: Ensembl
  3. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 559540Extracellular matrix protein 1PRO_0000021147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi463 – 4631N-linked (GlcNAc...) (high mannose)1 Publication
Glycosylationi535 – 5351N-linked (GlcNAc...) (high mannose)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ61508.
PaxDbiQ61508.
PRIDEiQ61508.

PTM databases

PhosphoSiteiQ61508.

Expressioni

Tissue specificityi

Expressed in the connective tissues surronding developing long bones, but not in the cartilage. The long isoform is expressed in a number of tissues including liver, heart and lungs. The short isoform is expressed in skin and cartilage-containing tissues such as tail and front paw. No expression is found in brain.1 Publication

Gene expression databases

BgeeiQ61508.
CleanExiMM_ECM1.
ExpressionAtlasiQ61508. baseline and differential.
GenevestigatoriQ61508.

Interactioni

Subunit structurei

Interacts (via C-terminus) with HSPG2 (via C-terminus). Interacts with EFEMP1/FBLN3 and LAMB3. Interacts with MMP9.By similarity

Protein-protein interaction databases

IntActiQ61508. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ61508.
SMRiQ61508. Positions 213-299, 365-423, 440-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati170 – 2981291Add
BLAST
Repeati302 – 4241232Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 4242552 X approximate repeatsAdd
BLAST

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG45869.
GeneTreeiENSGT00390000006215.
HOGENOMiHOG000112317.
HOVERGENiHBG005561.
InParanoidiQ61508.
OMAiCCHYPPS.
OrthoDBiEOG7KWSHX.
PhylomeDBiQ61508.
TreeFamiTF330786.

Family and domain databases

InterProiIPR008605. ECM1.
IPR020858. Serum_albumin-like.
[Graphical view]
PfamiPF05782. ECM1. 1 hit.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q61508-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTVSRAALI LACLALASAA SEGAFKASDQ REMTPERLFQ HLHEVGYAAP
60 70 80 90 100
PSPPQTRRLR VDHSVTSLHD PPLFEEQREV QPPSSPEDIP VYEEDWPTFL
110 120 130 140 150
NPNVDKAGPA VPQEAIPLQK EQPPPQVHIE QKEIDPPAQP QEEIVQKEVK
160 170 180 190 200
PHTLAGQLPP EPRTWNPARH CQQGRRGVWG HRLDGFPPGR PSPDNLKQIC
210 220 230 240 250
LPERQHVIYG PWNLPQTGYS HLSRQGETLN VLETGYSRCC RCRSDTNRLD
260 270 280 290 300
CLKLVWEDAM TQFCEAEFSV KTRPHLCCRL RGEERFSCFQ KEAPRPDYLL
310 320 330 340 350
RPCPVHQNGM SSGPQLPFPP GLPTPDNVKN ICLLRRFRAV PRNLPATDAI
360 370 380 390 400
QRQLQALTRL ETEFQRCCRQ GHNHTCTWKA WEGTLDGYCE RELAIKTHPH
410 420 430 440 450
SCCHYPPSPA RDECFAHLAP YPNYDRDILT LDLSRVTPNL MGQLCGSGRV
460 470 480 490 500
LSKHKQIPGL IQNMTIRCCE LPYPEQACCG EEEKLAFIEN LCGPRRNSWK
510 520 530 540 550
DPALCCDLSP EDKQINCFNT NYLRNVALVA GDTGNATGLG EQGPTRGTDA

NPAPGSKEE
Length:559
Mass (Da):62,832
Last modified:July 27, 2011 - v2
Checksum:iF5282E5D7B78E696
GO
Isoform Short (identifier: Q61508-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-380: Missing.

Show »
Length:434
Mass (Da):48,357
Checksum:iFB6101384A5F7150
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531P → L in AAA37535. (PubMed:7608209)Curated
Sequence conflicti241 – 2411R → P in AAA37535. (PubMed:7608209)Curated
Sequence conflicti466 – 4661I → V in AAA37535. (PubMed:7608209)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei256 – 380125Missing in isoform Short. 1 PublicationVSP_004230Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33416 mRNA. Translation: AAA37535.1.
AK153009 mRNA. Translation: BAE31648.1.
AK153471 mRNA. Translation: BAE32022.1.
CCDSiCCDS17619.1. [Q61508-1]
RefSeqiNP_031925.2. NM_007899.2. [Q61508-1]
UniGeneiMm.3433.

Genome annotation databases

EnsembliENSMUST00000117507; ENSMUSP00000112665; ENSMUSG00000028108. [Q61508-1]
GeneIDi13601.
KEGGimmu:13601.
UCSCiuc008qko.2. mouse. [Q61508-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33416 mRNA. Translation: AAA37535.1 .
AK153009 mRNA. Translation: BAE31648.1 .
AK153471 mRNA. Translation: BAE32022.1 .
CCDSi CCDS17619.1. [Q61508-1 ]
RefSeqi NP_031925.2. NM_007899.2. [Q61508-1 ]
UniGenei Mm.3433.

3D structure databases

ProteinModelPortali Q61508.
SMRi Q61508. Positions 213-299, 365-423, 440-482.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q61508. 1 interaction.

PTM databases

PhosphoSitei Q61508.

Proteomic databases

MaxQBi Q61508.
PaxDbi Q61508.
PRIDEi Q61508.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000117507 ; ENSMUSP00000112665 ; ENSMUSG00000028108 . [Q61508-1 ]
GeneIDi 13601.
KEGGi mmu:13601.
UCSCi uc008qko.2. mouse. [Q61508-1 ]

Organism-specific databases

CTDi 1893.
MGIi MGI:103060. Ecm1.

Phylogenomic databases

eggNOGi NOG45869.
GeneTreei ENSGT00390000006215.
HOGENOMi HOG000112317.
HOVERGENi HBG005561.
InParanoidi Q61508.
OMAi CCHYPPS.
OrthoDBi EOG7KWSHX.
PhylomeDBi Q61508.
TreeFami TF330786.

Miscellaneous databases

NextBioi 284228.
PROi Q61508.
SOURCEi Search...

Gene expression databases

Bgeei Q61508.
CleanExi MM_ECM1.
ExpressionAtlasi Q61508. baseline and differential.
Genevestigatori Q61508.

Family and domain databases

InterProi IPR008605. ECM1.
IPR020858. Serum_albumin-like.
[Graphical view ]
Pfami PF05782. ECM1. 1 hit.
[Graphical view ]
SUPFAMi SSF48552. SSF48552. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, characterization, and genetic mapping of the cDNA coding for a novel secretory protein of mouse. Demonstration of alternative splicing in skin and cartilage."
    Bhalerao J., Tylzanowski P., Filie J.D., Kozak C.A., Merregaert J.
    J. Biol. Chem. 270:16385-16394(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), PROTEIN SEQUENCE OF 20-37; 71-83 AND 109-112.
    Strain: BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  3. "Recombinant human extracellular matrix protein 1 inhibits alkaline phosphatase activity and mineralization of mouse embryonic metatarsals in vitro."
    Deckers M.M.L., Smits P., Karperien M., Ni J., Tylzanowski P., Feng P., Parmelee D., Zhang J., Bouffard E., Gentz R., Loewik C.W.G.M., Merregaert J.
    Bone 28:14-20(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis."
    Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S.
    Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-463 AND ASN-535.
    Tissue: Epidermis.

Entry informationi

Entry nameiECM1_MOUSE
AccessioniPrimary (citable) accession number: Q61508
Secondary accession number(s): Q3U5Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3