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Protein

Extracellular matrix protein 1

Gene

Ecm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • biomineral tissue development Source: UniProtKB-KW
  • inflammatory response Source: MGI
  • negative regulation of bone mineralization Source: UniProtKB
  • negative regulation of cytokine-mediated signaling pathway Source: MGI
  • negative regulation of peptidase activity Source: UniProtKB
  • ossification Source: UniProtKB-KW
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of endothelial cell proliferation Source: UniProtKB
  • regulation of T cell migration Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • regulation of type 2 immune response Source: MGI
  • signal transduction Source: InterPro

Keywordsi

Biological processAngiogenesis, Biomineralization, Mineral balance, Osteogenesis

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular matrix protein 1
Alternative name(s):
Secretory component p85
Gene namesi
Name:Ecm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:103060 Ecm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000002114720 – 559Extracellular matrix protein 1Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi373N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi463N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Glycosylationi535N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Modified residuei556PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ61508
PeptideAtlasiQ61508
PRIDEiQ61508

PTM databases

iPTMnetiQ61508
PhosphoSitePlusiQ61508
SwissPalmiQ61508
UniCarbKBiQ61508

Expressioni

Tissue specificityi

Expressed in the connective tissues surronding developing long bones, but not in the cartilage. The long isoform is expressed in a number of tissues including liver, heart and lungs. The short isoform is expressed in skin and cartilage-containing tissues such as tail and front paw. No expression is found in brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000028108
CleanExiMM_ECM1
ExpressionAtlasiQ61508 baseline and differential
GenevisibleiQ61508 MM

Interactioni

Subunit structurei

Interacts (via C-terminus) with HSPG2 (via C-terminus). Interacts with EFEMP1/FBLN3 and LAMB3. Interacts with MMP9.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ61508, 1 interactor
STRINGi10090.ENSMUSP00000112665

Structurei

3D structure databases

ProteinModelPortaliQ61508
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati170 – 2981Add BLAST129
Repeati302 – 4242Add BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 4242 X approximate repeatsAdd BLAST255

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IVN8 Eukaryota
ENOG4111TGN LUCA
GeneTreeiENSGT00390000006215
HOGENOMiHOG000112317
HOVERGENiHBG005561
InParanoidiQ61508
OMAiCCHYPPS
OrthoDBiEOG091G0NAE
PhylomeDBiQ61508
TreeFamiTF330786

Family and domain databases

InterProiView protein in InterPro
IPR008605 ECM1
IPR020858 Serum_albumin-like
PANTHERiPTHR16776 PTHR16776, 1 hit
PfamiView protein in Pfam
PF05782 ECM1, 1 hit
SUPFAMiSSF48552 SSF48552, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q61508-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTVSRAALI LACLALASAA SEGAFKASDQ REMTPERLFQ HLHEVGYAAP
60 70 80 90 100
PSPPQTRRLR VDHSVTSLHD PPLFEEQREV QPPSSPEDIP VYEEDWPTFL
110 120 130 140 150
NPNVDKAGPA VPQEAIPLQK EQPPPQVHIE QKEIDPPAQP QEEIVQKEVK
160 170 180 190 200
PHTLAGQLPP EPRTWNPARH CQQGRRGVWG HRLDGFPPGR PSPDNLKQIC
210 220 230 240 250
LPERQHVIYG PWNLPQTGYS HLSRQGETLN VLETGYSRCC RCRSDTNRLD
260 270 280 290 300
CLKLVWEDAM TQFCEAEFSV KTRPHLCCRL RGEERFSCFQ KEAPRPDYLL
310 320 330 340 350
RPCPVHQNGM SSGPQLPFPP GLPTPDNVKN ICLLRRFRAV PRNLPATDAI
360 370 380 390 400
QRQLQALTRL ETEFQRCCRQ GHNHTCTWKA WEGTLDGYCE RELAIKTHPH
410 420 430 440 450
SCCHYPPSPA RDECFAHLAP YPNYDRDILT LDLSRVTPNL MGQLCGSGRV
460 470 480 490 500
LSKHKQIPGL IQNMTIRCCE LPYPEQACCG EEEKLAFIEN LCGPRRNSWK
510 520 530 540 550
DPALCCDLSP EDKQINCFNT NYLRNVALVA GDTGNATGLG EQGPTRGTDA

NPAPGSKEE
Length:559
Mass (Da):62,832
Last modified:July 27, 2011 - v2
Checksum:iF5282E5D7B78E696
GO
Isoform Short (identifier: Q61508-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-380: Missing.

Show »
Length:434
Mass (Da):48,357
Checksum:iFB6101384A5F7150
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53P → L in AAA37535 (PubMed:7608209).Curated1
Sequence conflicti241R → P in AAA37535 (PubMed:7608209).Curated1
Sequence conflicti466I → V in AAA37535 (PubMed:7608209).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004230256 – 380Missing in isoform Short. 1 PublicationAdd BLAST125

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33416 mRNA Translation: AAA37535.1
AK153009 mRNA Translation: BAE31648.1
AK153471 mRNA Translation: BAE32022.1
CCDSiCCDS17619.1 [Q61508-1]
RefSeqiNP_031925.2, NM_007899.2 [Q61508-1]
UniGeneiMm.3433

Genome annotation databases

EnsembliENSMUST00000117507; ENSMUSP00000112665; ENSMUSG00000028108 [Q61508-1]
GeneIDi13601
KEGGimmu:13601
UCSCiuc008qko.2 mouse [Q61508-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiECM1_MOUSE
AccessioniPrimary (citable) accession number: Q61508
Secondary accession number(s): Q3U5Q5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health