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Q61508

- ECM1_MOUSE

UniProt

Q61508 - ECM1_MOUSE

Protein

Extracellular matrix protein 1

Gene

Ecm1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity By similarity.By similarity

    GO - Molecular functioni

    1. interleukin-2 receptor binding Source: MGI
    2. signal transducer activity Source: Ensembl

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. biomineral tissue development Source: UniProtKB-KW
    3. inflammatory response Source: MGI
    4. negative regulation of bone mineralization Source: UniProtKB
    5. negative regulation of cytokine-mediated signaling pathway Source: MGI
    6. negative regulation of peptidase activity Source: UniProtKB
    7. ossification Source: UniProtKB-KW
    8. positive regulation of angiogenesis Source: UniProtKB
    9. positive regulation of endothelial cell proliferation Source: UniProtKB
    10. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    11. regulation of T cell migration Source: MGI
    12. regulation of transcription from RNA polymerase II promoter Source: MGI
    13. regulation of type 2 immune response Source: MGI

    Keywords - Biological processi

    Angiogenesis, Biomineralization, Mineral balance, Osteogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Extracellular matrix protein 1
    Alternative name(s):
    Secretory component p85
    Gene namesi
    Name:Ecm1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:103060. Ecm1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI
    2. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – 559540Extracellular matrix protein 1PRO_0000021147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi463 – 4631N-linked (GlcNAc...) (high mannose)1 Publication
    Glycosylationi535 – 5351N-linked (GlcNAc...) (high mannose)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ61508.
    PaxDbiQ61508.
    PRIDEiQ61508.

    PTM databases

    PhosphoSiteiQ61508.

    Expressioni

    Tissue specificityi

    Expressed in the connective tissues surronding developing long bones, but not in the cartilage. The long isoform is expressed in a number of tissues including liver, heart and lungs. The short isoform is expressed in skin and cartilage-containing tissues such as tail and front paw. No expression is found in brain.1 Publication

    Gene expression databases

    ArrayExpressiQ61508.
    BgeeiQ61508.
    CleanExiMM_ECM1.
    GenevestigatoriQ61508.

    Interactioni

    Subunit structurei

    Interacts (via C-terminus) with HSPG2 (via C-terminus). Interacts with EFEMP1/FBLN3 and LAMB3. Interacts with MMP9.By similarity

    Protein-protein interaction databases

    IntActiQ61508. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61508.
    SMRiQ61508. Positions 213-299, 365-423, 440-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati170 – 2981291Add
    BLAST
    Repeati302 – 4241232Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni170 – 4242552 X approximate repeatsAdd
    BLAST

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG45869.
    GeneTreeiENSGT00390000006215.
    HOGENOMiHOG000112317.
    HOVERGENiHBG005561.
    InParanoidiQ3U5Q5.
    OMAiCCHYPPS.
    OrthoDBiEOG7KWSHX.
    PhylomeDBiQ61508.
    TreeFamiTF330786.

    Family and domain databases

    InterProiIPR008605. ECM1.
    IPR020858. Serum_albumin-like.
    [Graphical view]
    PfamiPF05782. ECM1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48552. SSF48552. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q61508-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGTVSRAALI LACLALASAA SEGAFKASDQ REMTPERLFQ HLHEVGYAAP    50
    PSPPQTRRLR VDHSVTSLHD PPLFEEQREV QPPSSPEDIP VYEEDWPTFL 100
    NPNVDKAGPA VPQEAIPLQK EQPPPQVHIE QKEIDPPAQP QEEIVQKEVK 150
    PHTLAGQLPP EPRTWNPARH CQQGRRGVWG HRLDGFPPGR PSPDNLKQIC 200
    LPERQHVIYG PWNLPQTGYS HLSRQGETLN VLETGYSRCC RCRSDTNRLD 250
    CLKLVWEDAM TQFCEAEFSV KTRPHLCCRL RGEERFSCFQ KEAPRPDYLL 300
    RPCPVHQNGM SSGPQLPFPP GLPTPDNVKN ICLLRRFRAV PRNLPATDAI 350
    QRQLQALTRL ETEFQRCCRQ GHNHTCTWKA WEGTLDGYCE RELAIKTHPH 400
    SCCHYPPSPA RDECFAHLAP YPNYDRDILT LDLSRVTPNL MGQLCGSGRV 450
    LSKHKQIPGL IQNMTIRCCE LPYPEQACCG EEEKLAFIEN LCGPRRNSWK 500
    DPALCCDLSP EDKQINCFNT NYLRNVALVA GDTGNATGLG EQGPTRGTDA 550
    NPAPGSKEE 559
    Length:559
    Mass (Da):62,832
    Last modified:July 27, 2011 - v2
    Checksum:iF5282E5D7B78E696
    GO
    Isoform Short (identifier: Q61508-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         256-380: Missing.

    Show »
    Length:434
    Mass (Da):48,357
    Checksum:iFB6101384A5F7150
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531P → L in AAA37535. (PubMed:7608209)Curated
    Sequence conflicti241 – 2411R → P in AAA37535. (PubMed:7608209)Curated
    Sequence conflicti466 – 4661I → V in AAA37535. (PubMed:7608209)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei256 – 380125Missing in isoform Short. 1 PublicationVSP_004230Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33416 mRNA. Translation: AAA37535.1.
    AK153009 mRNA. Translation: BAE31648.1.
    AK153471 mRNA. Translation: BAE32022.1.
    CCDSiCCDS17619.1. [Q61508-1]
    RefSeqiNP_031925.2. NM_007899.2. [Q61508-1]
    UniGeneiMm.3433.

    Genome annotation databases

    EnsembliENSMUST00000117507; ENSMUSP00000112665; ENSMUSG00000028108. [Q61508-1]
    GeneIDi13601.
    KEGGimmu:13601.
    UCSCiuc008qko.2. mouse. [Q61508-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33416 mRNA. Translation: AAA37535.1 .
    AK153009 mRNA. Translation: BAE31648.1 .
    AK153471 mRNA. Translation: BAE32022.1 .
    CCDSi CCDS17619.1. [Q61508-1 ]
    RefSeqi NP_031925.2. NM_007899.2. [Q61508-1 ]
    UniGenei Mm.3433.

    3D structure databases

    ProteinModelPortali Q61508.
    SMRi Q61508. Positions 213-299, 365-423, 440-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q61508. 1 interaction.

    PTM databases

    PhosphoSitei Q61508.

    Proteomic databases

    MaxQBi Q61508.
    PaxDbi Q61508.
    PRIDEi Q61508.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000117507 ; ENSMUSP00000112665 ; ENSMUSG00000028108 . [Q61508-1 ]
    GeneIDi 13601.
    KEGGi mmu:13601.
    UCSCi uc008qko.2. mouse. [Q61508-1 ]

    Organism-specific databases

    CTDi 1893.
    MGIi MGI:103060. Ecm1.

    Phylogenomic databases

    eggNOGi NOG45869.
    GeneTreei ENSGT00390000006215.
    HOGENOMi HOG000112317.
    HOVERGENi HBG005561.
    InParanoidi Q3U5Q5.
    OMAi CCHYPPS.
    OrthoDBi EOG7KWSHX.
    PhylomeDBi Q61508.
    TreeFami TF330786.

    Miscellaneous databases

    NextBioi 284228.
    PROi Q61508.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61508.
    Bgeei Q61508.
    CleanExi MM_ECM1.
    Genevestigatori Q61508.

    Family and domain databases

    InterProi IPR008605. ECM1.
    IPR020858. Serum_albumin-like.
    [Graphical view ]
    Pfami PF05782. ECM1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48552. SSF48552. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, characterization, and genetic mapping of the cDNA coding for a novel secretory protein of mouse. Demonstration of alternative splicing in skin and cartilage."
      Bhalerao J., Tylzanowski P., Filie J.D., Kozak C.A., Merregaert J.
      J. Biol. Chem. 270:16385-16394(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), PROTEIN SEQUENCE OF 20-37; 71-83 AND 109-112.
      Strain: BALB/c.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Strain: C57BL/6J.
      Tissue: Bone marrow.
    3. "Recombinant human extracellular matrix protein 1 inhibits alkaline phosphatase activity and mineralization of mouse embryonic metatarsals in vitro."
      Deckers M.M.L., Smits P., Karperien M., Ni J., Tylzanowski P., Feng P., Parmelee D., Zhang J., Bouffard E., Gentz R., Loewik C.W.G.M., Merregaert J.
      Bone 28:14-20(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis."
      Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S.
      Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-463 AND ASN-535.
      Tissue: Epidermis.

    Entry informationi

    Entry nameiECM1_MOUSE
    AccessioniPrimary (citable) accession number: Q61508
    Secondary accession number(s): Q3U5Q5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3