Reviewed, UniProtKB/Swiss-Prot Q61503 (5NTD_MOUSE)

Names and origin

Protein names

Recommended name:
    5'-nucleotidase
      Short name=5'-NT
    EC=3.1.3.5
Alternative name(s):
    Ecto-5'-nucleotidase
    CD_antigen=CD73

Gene names

Name:	Nt5e
Synonyms:	Nt5, Nte

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Zinc By similarity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the 5'-nucleotidase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	AMP catabolic process Inferred from mutant phenotype. Source: MGI adenosine biosynthetic process Inferred from mutant phenotype. Source: MGI negative regulation of inflammatory response Inferred from mutant phenotype. Source: MGI
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Inferred from direct assay. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5'-nucleotidase activity Inferred from mutant phenotype. Source: MGI nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	By similarity
	Chain	29 – 551	523	5'-nucleotidase		PRO_0000000017
	Propeptide	552 – 576	25	Removed in mature form By similarity		PRO_0000000018
Regions
	Region	502 – 508	7	Substrate binding By similarity
Sites
	Metal binding	38	1	Zinc 1 By similarity
	Metal binding	40	1	Zinc 1 By similarity
	Metal binding	87	1	Zinc 1 By similarity
	Metal binding	87	1	Zinc 2 By similarity
	Metal binding	119	1	Zinc 2 By similarity
	Metal binding	222	1	Zinc 2 By similarity
	Metal binding	245	1	Zinc 2 By similarity
	Binding site	419	1	Substrate By similarity
	Site	120	1	Transition state stabilizer By similarity
	Site	123	1	Transition state stabilizer By similarity
Amino acid modifications
	Lipidation	551	1	GPI-anchor amidated serine By similarity
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	313	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	335	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	405	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q61503-1 [UniParc]. Last modified August 1, 1998. Version 2. Checksum: 29D697928A5D5915	FASTA	576	63,864
	10 20 30 40 50 60 MRPAAAKVPK WLLLALSALL PQWPAASAWE LTILHTNDVH SRLEQTSDDS TKCLNASLCV 70 80 90 100 110 120 GGVARLFTKV QQIRKEEPNV LFLDAGDQYQ GTIWFTVYKG LEVAHFMNIL GYDAMALGNH 130 140 150 160 170 180 EFDNGVEGLI DPLLRNVKFP ILSANIKARG PLAHQISGLF LPSKVLSVGG EVVGIVGYTS 190 200 210 220 230 240 KETPFLSNPG TNLVFEDEIS ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDI 250 260 270 280 290 300 VVGGHSNTFL YTGNPPSKEV PAGKYPFIVT ADDGRQVPVV QAYAFGKYLG YLKVEFDDKG 310 320 330 340 350 360 NVITSYGNPI LLNSSIPEDA TIKADINQWR IKLDNYSTQE LGRTIVYLDG STQTCRFREC 370 380 390 400 410 420 NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI DEKNNGTITW ENLAAVLPFG 430 440 450 460 470 480 GTFDLVQLKG STLKKAFEHS VHRYGQSTGE FLQVGGIHVV YDINRKPWNR VVQLEVLCTK 490 500 510 520 530 540 CRVPIYEPLE MDKVYKVTLP SYLANGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVV 550 560 570 YPAVEGRIKF SAASHYQGSF PLVILSFWAM ILILYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Murine ecto-5'-nucleotidase (CD73): cDNA cloning and tissue distribution." Resta R., Hooker S.W., Hansen K.R., Laurent A.B., Park J.L., Blackburn M.R., Knudsen T.B., Thompson L.F. Gene 133:171-177(1993) [PubMed: 8224905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Kidney.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Skin and Testis.
[3]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-313 AND ASN-335, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12059 mRNA. Translation: AAC13542.1. AK028723 mRNA. Translation: BAC26084.1. AK029979 mRNA. Translation: BAC26714.1. AK154614 mRNA. Translation: BAE32714.1.
IPI	IPI00122257.
PIR	JC2001.
RefSeq	NP_035981.1.
UniGene	Mm.244235
3D structure databases
SMR	Q61503. Positions 29-552.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61503.
Proteomic databases
PRIDE	Q61503.
Genome annotation databases
Ensembl	ENSMUST00000034992; ENSMUSP00000034992; ENSMUSG00000032420; Mus musculus. [Genome view]
GeneID	23959.
KEGG	mmu:23959.
NMPDR	fig|10090.3.peg.20616.
UCSC	uc009qyj.1. mouse.
Organism-specific databases
CTD	23959.
MGI	MGI:99782. Nt5e.
Phylogenomic databases
HOGENOM	HBG534106.
HOVERGEN	Q61503.
InParanoid	Q61503.
OMA	KDELLRH.
OrthoDB	EOG92NMKC.
PhylomeDB	Q61503.
Enzyme and pathway databases
BRENDA	3.1.3.5. 244.
Gene expression databases
ArrayExpress	Q61503.
Bgee	Q61503.
CleanEx	MM_NT5E.
Genevestigator	Q61503.
GermOnline	ENSMUSG00000032420. Mus musculus.
Family and domain databases
InterPro	IPR008334. 5'-Nucleotdase_C. IPR006146. 5'-Nucleotdase_CS. IPR006179. 5_nucleotidase/apyrase. IPR004843. M-pesterase. [Graphical view]
Gene3D	G3DSA:3.90.780.10. 5'-Nucleotdase_C. 1 hit.
PANTHER	PTHR11575. 5_nucleotidase. 1 hit.
Pfam	PF02872. 5_nucleotid_C. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR01607. APYRASEFAMLY.
PROSITE	PS00785. 5_NUCLEOTIDASE_1. 1 hit. PS00786. 5_NUCLEOTIDASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	303800.
SOURCE	Search...

Entry information

Entry name

5NTD_MOUSE

Accession

Primary (citable) accession number: Q61503
Secondary accession number(s): Q3U3S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)