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Protein

5'-nucleotidase

Gene

Nt5e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Zinc 1By similarity1
Metal bindingi40Zinc 1By similarity1
Metal bindingi87Zinc 1By similarity1
Metal bindingi87Zinc 2By similarity1
Metal bindingi119Zinc 2By similarity1
Sitei120Transition state stabilizerBy similarity1
Sitei123Transition state stabilizerBy similarity1
Metal bindingi222Zinc 2By similarity1
Metal bindingi245Zinc 2By similarity1
Binding sitei247SubstrateBy similarity1
Binding sitei356SubstrateBy similarity1
Binding sitei392SubstrateBy similarity1
Binding sitei397SubstrateBy similarity1
Binding sitei419SubstrateBy similarity1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • adenosine biosynthetic process Source: MGI
  • AMP catabolic process Source: MGI
  • leukocyte cell-cell adhesion Source: MGI
  • negative regulation of inflammatory response Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-73621. Pyrimidine catabolism.
R-MMU-74259. Purine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Alternative name(s):
Ecto-5'-nucleotidase
CD_antigen: CD73
Gene namesi
Name:Nt5e
Synonyms:Nt5, Nte
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:99782. Nt5e.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • cell surface Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28By similarityAdd BLAST28
ChainiPRO_000000001729 – 5515'-nucleotidaseAdd BLAST523
PropeptideiPRO_0000000018552 – 576Removed in mature formBy similarityAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 59By similarity
Glycosylationi55N-linked (GlcNAc...)Sequence analysis1
Glycosylationi313N-linked (GlcNAc...)1 Publication1
Glycosylationi335N-linked (GlcNAc...)1 Publication1
Disulfide bondi355 ↔ 360By similarity
Disulfide bondi367 ↔ 389By similarity
Glycosylationi405N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi478 ↔ 481By similarity
Lipidationi551GPI-anchor amidated serineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ61503.
PaxDbiQ61503.
PeptideAtlasiQ61503.
PRIDEiQ61503.

PTM databases

iPTMnetiQ61503.
PhosphoSitePlusiQ61503.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032420.
CleanExiMM_NT5E.
ExpressionAtlasiQ61503. baseline and differential.
GenevisibleiQ61503. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ61503. 3 interactors.
MINTiMINT-4086307.
STRINGi10090.ENSMUSP00000034992.

Structurei

3D structure databases

ProteinModelPortaliQ61503.
SMRiQ61503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni502 – 508Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4419. Eukaryota.
COG0737. LUCA.
GeneTreeiENSGT00530000063775.
HOGENOMiHOG000247215.
HOVERGENiHBG000026.
InParanoidiQ61503.
KOiK19970.
OMAiLFHTIAR.
OrthoDBiEOG091G08IK.
PhylomeDBiQ61503.
TreeFamiTF323589.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPAAAKVPK WLLLALSALL PQWPAASAWE LTILHTNDVH SRLEQTSDDS
60 70 80 90 100
TKCLNASLCV GGVARLFTKV QQIRKEEPNV LFLDAGDQYQ GTIWFTVYKG
110 120 130 140 150
LEVAHFMNIL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG
160 170 180 190 200
PLAHQISGLF LPSKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEIS
210 220 230 240 250
ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDI VVGGHSNTFL
260 270 280 290 300
YTGNPPSKEV PAGKYPFIVT ADDGRQVPVV QAYAFGKYLG YLKVEFDDKG
310 320 330 340 350
NVITSYGNPI LLNSSIPEDA TIKADINQWR IKLDNYSTQE LGRTIVYLDG
360 370 380 390 400
STQTCRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI
410 420 430 440 450
DEKNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE
460 470 480 490 500
FLQVGGIHVV YDINRKPWNR VVQLEVLCTK CRVPIYEPLE MDKVYKVTLP
510 520 530 540 550
SYLANGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVV YPAVEGRIKF
560 570
SAASHYQGSF PLVILSFWAM ILILYQ
Length:576
Mass (Da):63,864
Last modified:August 1, 1998 - v2
Checksum:i29D697928A5D5915
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12059 mRNA. Translation: AAC13542.1.
AK028723 mRNA. Translation: BAC26084.1.
AK029979 mRNA. Translation: BAC26714.1.
AK154614 mRNA. Translation: BAE32714.1.
CCDSiCCDS23386.1.
PIRiJC2001.
RefSeqiNP_035981.1. NM_011851.4.
UniGeneiMm.244235.

Genome annotation databases

EnsembliENSMUST00000034992; ENSMUSP00000034992; ENSMUSG00000032420.
GeneIDi23959.
KEGGimmu:23959.
UCSCiuc009qyj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12059 mRNA. Translation: AAC13542.1.
AK028723 mRNA. Translation: BAC26084.1.
AK029979 mRNA. Translation: BAC26714.1.
AK154614 mRNA. Translation: BAE32714.1.
CCDSiCCDS23386.1.
PIRiJC2001.
RefSeqiNP_035981.1. NM_011851.4.
UniGeneiMm.244235.

3D structure databases

ProteinModelPortaliQ61503.
SMRiQ61503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61503. 3 interactors.
MINTiMINT-4086307.
STRINGi10090.ENSMUSP00000034992.

PTM databases

iPTMnetiQ61503.
PhosphoSitePlusiQ61503.

Proteomic databases

MaxQBiQ61503.
PaxDbiQ61503.
PeptideAtlasiQ61503.
PRIDEiQ61503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034992; ENSMUSP00000034992; ENSMUSG00000032420.
GeneIDi23959.
KEGGimmu:23959.
UCSCiuc009qyj.2. mouse.

Organism-specific databases

CTDi4907.
MGIiMGI:99782. Nt5e.

Phylogenomic databases

eggNOGiKOG4419. Eukaryota.
COG0737. LUCA.
GeneTreeiENSGT00530000063775.
HOGENOMiHOG000247215.
HOVERGENiHBG000026.
InParanoidiQ61503.
KOiK19970.
OMAiLFHTIAR.
OrthoDBiEOG091G08IK.
PhylomeDBiQ61503.
TreeFamiTF323589.

Enzyme and pathway databases

ReactomeiR-MMU-73621. Pyrimidine catabolism.
R-MMU-74259. Purine catabolism.

Miscellaneous databases

PROiQ61503.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032420.
CleanExiMM_NT5E.
ExpressionAtlasiQ61503. baseline and differential.
GenevisibleiQ61503. MM.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5NTD_MOUSE
AccessioniPrimary (citable) accession number: Q61503
Secondary accession number(s): Q3U3S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.