Reviewed, UniProtKB/Swiss-Prot Q61502 (E2F5_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcription factor E2F5
      Short name=E2F-5

Gene names

Name:

E2f5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction.
Subunit structure	Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 By similarity.
Subcellular location	Nucleus.
Developmental stage	In the developing epidermis, first detected in 13.5-14.5 dpc embryos. With the appearance of stratified epithelium, levels of E2F-5 expression increase and by 16.5 dpc, high expression found in the suprabasal cell layers. High expression also found in other regions with stratified squamous epithelia including the developing palate, lip and tongue. In the developing nervous system, first detected in the forebrain at 9.5 dpc. At 10.5 dpc, strongly expressed in the rostral region of the spinal cord. By 11.5 dpc, E2F-5 is expressed throughout the developing central nervous system. In 12.5-15.5 dpc embryos, expression found in the undifferentiated ventricular regions of the brain. In the retina, expressed, in 14.5-18.5 dpc embryos, in the retinoblastic cell layer. In other developing tissues, highly expressed in the choroid plexus. Also found in the kidney, liver, lung, heart and weakly, in developing skeletal muscle and chondrocytes. Ref.2 Ref.3
Sequence similarities	Belongs to the E2F/DP family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Traceable author statement. Source: MGI organ morphogenesis Inferred from mutant phenotype. Source: MGI regulation of cell cycle Traceable author statement. Source: MGI regulation of transcription, DNA-dependent Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nuclear envelope Traceable author statement. Source: MGI transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: MGI transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Transcription factor E2F5		PRO_0000219470
Regions
	Domain	66 – 88	23	Leucine-zipper
	DNA binding	37 – 108	72	Potential
	Region	109 – 205	97	Dimerization Potential
	Region	277 – 335	59	Transactivation Potential
	Region	312 – 329	18	RBL2 association Potential
	Motif	71 – 108	38	DEF box
	Compositional bias	223 – 226	4	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q61502-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EAFCCD5B9AF5DBE7	FASTA	335	36,784
	10 20 30 40 50 60 MAAAEPTSSA QPTPQAQAQP PPHGAPSSQP SRRSRGGSSR HEKSLGLLTT KFVSLLQEAQ 70 80 90 100 110 120 DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN SIQWKGVGAG CNTKEVIDRL 130 140 150 160 170 180 RCLKAEIEDL ELKERELDQQ KLWLQQSIKN VMEDSINNRF SYVTHEDICN CFHGDTLLAI 190 200 210 220 230 240 QAPSGTQLEV PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT 250 260 270 280 290 300 QPSSQSSTSV TPQKSTMAAQ NLPEQHVSER SQTFQQTPAA EVSSGSISGD IIDELMSSDV 310 320 330 FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY

References

[1]	"Molecular and functional characterisation of E2F-5, a new member of the E2F family." Buck V., Allen K.E., Soerensen T., Bybee A., Hijmans E.M., Voorhoeve P.M., Bernards R., la Thangue N.B. Oncogene 11:31-38(1995) [PubMed: 7542760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression patterns of the E2F family of transcription factors during mouse nervous system development." Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A. Mech. Dev. 66:13-25(1997) [PubMed: 9376316] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[3]	"Expression patterns of the E2F family of transcription factors during murine epithelial development." Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A. Cell Growth Differ. 8:553-563(1997) [PubMed: 9149906] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X86925 mRNA. Translation: CAA60508.1.
IPI	IPI00331569.
PIR	I48338.
UniGene	Mm.153415
3D structure databases
HSSP	HSSP built from PDB template 1CF7 based on UniProtKB Q16254.
SMR	Q61502. Positions 39-105.
ModBase	Search...
PTM databases
PhosphoSite	Q61502.
Genome annotation databases
Ensembl	ENSMUSG00000027552. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:105091. E2f5.
Phylogenomic databases
HOGENOM	Q61502.
HOVERGEN	Q61502.
Gene expression databases
ArrayExpress	Q61502.
Bgee	Q61502.
CleanEx	MM_E2F5.
GermOnline	ENSMUSG00000027552. Mus musculus.
Family and domain databases
InterPro	IPR015633. E2F. IPR003316. E2F_TDP. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR12081. E2F. 1 hit.
Pfam	PF02319. E2F_TDP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

E2F5_MOUSE

Accession

Primary (citable) accession number: Q61502

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)