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Q61502

- E2F5_MOUSE

UniProt

Q61502 - E2F5_MOUSE

Protein

Transcription factor E2F5

Gene

E2f5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi37 – 10872Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: MGI
    2. organ morphogenesis Source: MGI
    3. regulation of cell cycle Source: MGI
    4. regulation of transcription, DNA-templated Source: MGI
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_199110. G0 and Early G1.
    REACT_203903. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor E2F5
    Short name:
    E2F-5
    Gene namesi
    Name:E2f5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:105091. E2f5.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nuclear envelope Source: MGI
    3. nucleus Source: MGI
    4. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Transcription factor E2F5PRO_0000219470Add
    BLAST

    Proteomic databases

    PRIDEiQ61502.

    PTM databases

    PhosphoSiteiQ61502.

    Expressioni

    Developmental stagei

    In the developing epidermis, first detected in 13.5-14.5 dpc embryos. With the appearance of stratified epithelium, levels of E2F5 expression increase and by 16.5 dpc, high expression found in the suprabasal cell layers. High expression also found in other regions with stratified squamous epithelia including the developing palate, lip and tongue. In the developing nervous system, first detected in the forebrain at 9.5 dpc. At 10.5 dpc, strongly expressed in the rostral region of the spinal cord. By 11.5 dpc, E2F5 is expressed throughout the developing central nervous system. In 12.5-15.5 dpc embryos, expression found in the undifferentiated ventricular regions of the brain. In the retina, expressed, in 14.5-18.5 dpc embryos, in the retinoblastic cell layer. In other developing tissues, highly expressed in the choroid plexus. Also found in the kidney, liver, lung, heart and weakly, in developing skeletal muscle and chondrocytes.2 Publications

    Gene expression databases

    ArrayExpressiQ61502.
    BgeeiQ61502.
    CleanExiMM_E2F5.
    GenevestigatoriQ61502.

    Interactioni

    Subunit structurei

    Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Tfdp2Q64163-42EBI-7225685,EBI-8077763

    Protein-protein interaction databases

    BioGridi199352. 2 interactions.
    IntActiQ61502. 3 interactions.
    MINTiMINT-1784965.
    STRINGi10090.ENSMUSP00000029069.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61502.
    SMRiQ61502. Positions 39-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni66 – 8823Leucine-zipperAdd
    BLAST
    Regioni109 – 20597DimerizationSequence AnalysisAdd
    BLAST
    Regioni277 – 33559TransactivationSequence AnalysisAdd
    BLAST
    Regioni312 – 32918RBL2 associationSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi71 – 10838DEF boxAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi223 – 2264Poly-Ser

    Sequence similaritiesi

    Belongs to the E2F/DP family.Curated

    Phylogenomic databases

    eggNOGiNOG289227.
    GeneTreeiENSGT00550000074403.
    HOGENOMiHOG000232045.
    HOVERGENiHBG002227.
    InParanoidiQ99LK0.
    KOiK04682.
    OrthoDBiEOG7WHHB1.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR015633. E2F.
    IPR028316. E2F5.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR12081. PTHR12081. 1 hit.
    PTHR12081:SF35. PTHR12081:SF35. 1 hit.
    PfamiPF02319. E2F_TDP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61502-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAEPTSSA QPTPQAQAQP PPHGAPSSQP SAALAGGSSR HEKSLGLLTT    50
    KFVSLLQEAQ DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN 100
    SIQWKGVGAG CNTKEVIDRL RCLKAEIEDL ELKERELDQQ KLWLQQSIKN 150
    VMEDSINNRF SYVTHEDICN CFHGDTLLAI QAPSGTQLEV PIPEMGQNGQ 200
    KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT QPSSQSSTSV 250
    TPQKSTMAAQ NLPEQHVSER SQTFQQTPAA EVSSGSISGD IIDELMSSDV 300
    FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY 335
    Length:335
    Mass (Da):36,555
    Last modified:July 27, 2011 - v2
    Checksum:i7922ACE79C73944B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti32 – 354AALA → RRSR in CAA60508. (PubMed:7542760)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86925 mRNA. Translation: CAA60508.1.
    AK156760 mRNA. Translation: BAE33842.1.
    BC003220 mRNA. Translation: AAH03220.1.
    CCDSiCCDS38391.1.
    PIRiI48338.
    RefSeqiNP_031918.2. NM_007892.2.
    UniGeneiMm.153415.

    Genome annotation databases

    EnsembliENSMUST00000029069; ENSMUSP00000029069; ENSMUSG00000027552.
    GeneIDi13559.
    KEGGimmu:13559.
    UCSCiuc008oqk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86925 mRNA. Translation: CAA60508.1 .
    AK156760 mRNA. Translation: BAE33842.1 .
    BC003220 mRNA. Translation: AAH03220.1 .
    CCDSi CCDS38391.1.
    PIRi I48338.
    RefSeqi NP_031918.2. NM_007892.2.
    UniGenei Mm.153415.

    3D structure databases

    ProteinModelPortali Q61502.
    SMRi Q61502. Positions 39-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199352. 2 interactions.
    IntActi Q61502. 3 interactions.
    MINTi MINT-1784965.
    STRINGi 10090.ENSMUSP00000029069.

    PTM databases

    PhosphoSitei Q61502.

    Proteomic databases

    PRIDEi Q61502.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029069 ; ENSMUSP00000029069 ; ENSMUSG00000027552 .
    GeneIDi 13559.
    KEGGi mmu:13559.
    UCSCi uc008oqk.1. mouse.

    Organism-specific databases

    CTDi 1875.
    MGIi MGI:105091. E2f5.

    Phylogenomic databases

    eggNOGi NOG289227.
    GeneTreei ENSGT00550000074403.
    HOGENOMi HOG000232045.
    HOVERGENi HBG002227.
    InParanoidi Q99LK0.
    KOi K04682.
    OrthoDBi EOG7WHHB1.

    Enzyme and pathway databases

    Reactomei REACT_199110. G0 and Early G1.
    REACT_203903. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.

    Miscellaneous databases

    NextBioi 284178.
    PROi Q61502.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61502.
    Bgeei Q61502.
    CleanExi MM_E2F5.
    Genevestigatori Q61502.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR015633. E2F.
    IPR028316. E2F5.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR12081. PTHR12081. 1 hit.
    PTHR12081:SF35. PTHR12081:SF35. 1 hit.
    Pfami PF02319. E2F_TDP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and functional characterisation of E2F-5, a new member of the E2F family."
      Buck V., Allen K.E., Soerensen T., Bybee A., Hijmans E.M., Voorhoeve P.M., Bernards R., la Thangue N.B.
      Oncogene 11:31-38(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
      Tissue: Spleen.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
      Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
      Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "Expression patterns of the E2F family of transcription factors during murine epithelial development."
      Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
      Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiE2F5_MOUSE
    AccessioniPrimary (citable) accession number: Q61502
    Secondary accession number(s): Q99LK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3