Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor E2F5

Gene

E2f5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 108Sequence analysisAdd BLAST72

GO - Molecular functioni

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • cell projection organization Source: UniProtKB-KW
  • G1/S transition of mitotic cell cycle Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cilium biogenesis/degradation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1538133. G0 and Early G1.
R-MMU-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-MMU-69231. Cyclin D associated events in G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F5
Short name:
E2F-5
Gene namesi
Name:E2f5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:105091. E2f5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nuclear envelope Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
  • transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194701 – 335Transcription factor E2F5Add BLAST335

Proteomic databases

MaxQBiQ61502.
PaxDbiQ61502.
PeptideAtlasiQ61502.
PRIDEiQ61502.

PTM databases

iPTMnetiQ61502.
PhosphoSitePlusiQ61502.

Expressioni

Developmental stagei

In the developing epidermis, first detected in 13.5-14.5 dpc embryos. With the appearance of stratified epithelium, levels of E2F5 expression increase and by 16.5 dpc, high expression found in the suprabasal cell layers. High expression also found in other regions with stratified squamous epithelia including the developing palate, lip and tongue. In the developing nervous system, first detected in the forebrain at 9.5 dpc. At 10.5 dpc, strongly expressed in the rostral region of the spinal cord. By 11.5 dpc, E2F5 is expressed throughout the developing central nervous system. In 12.5-15.5 dpc embryos, expression found in the undifferentiated ventricular regions of the brain. In the retina, expressed, in 14.5-18.5 dpc embryos, in the retinoblastic cell layer. In other developing tissues, highly expressed in the choroid plexus. Also found in the kidney, liver, lung, heart and weakly, in developing skeletal muscle and chondrocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000027552.
CleanExiMM_E2F5.
ExpressionAtlasiQ61502. baseline and differential.
GenevisibleiQ61502. MM.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tfdp2Q64163-42EBI-7225685,EBI-8077763

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199352. 4 interactors.
IntActiQ61502. 3 interactors.
MINTiMINT-1784965.
STRINGi10090.ENSMUSP00000029069.

Structurei

3D structure databases

ProteinModelPortaliQ61502.
SMRiQ61502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni66 – 88Leucine-zipperAdd BLAST23
Regioni109 – 205DimerizationSequence analysisAdd BLAST97
Regioni277 – 335TransactivationSequence analysisAdd BLAST59
Regioni312 – 329RBL2 associationSequence analysisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi71 – 108DEF boxAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi223 – 226Poly-Ser4

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiQ61502.
KOiK04682.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAEPTSSA QPTPQAQAQP PPHGAPSSQP SAALAGGSSR HEKSLGLLTT
60 70 80 90 100
KFVSLLQEAQ DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN
110 120 130 140 150
SIQWKGVGAG CNTKEVIDRL RCLKAEIEDL ELKERELDQQ KLWLQQSIKN
160 170 180 190 200
VMEDSINNRF SYVTHEDICN CFHGDTLLAI QAPSGTQLEV PIPEMGQNGQ
210 220 230 240 250
KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT QPSSQSSTSV
260 270 280 290 300
TPQKSTMAAQ NLPEQHVSER SQTFQQTPAA EVSSGSISGD IIDELMSSDV
310 320 330
FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY
Length:335
Mass (Da):36,555
Last modified:July 27, 2011 - v2
Checksum:i7922ACE79C73944B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32 – 35AALA → RRSR in CAA60508 (PubMed:7542760).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86925 mRNA. Translation: CAA60508.1.
AK156760 mRNA. Translation: BAE33842.1.
BC003220 mRNA. Translation: AAH03220.1.
CCDSiCCDS38391.1.
PIRiI48338.
RefSeqiNP_031918.2. NM_007892.2.
UniGeneiMm.153415.

Genome annotation databases

EnsembliENSMUST00000029069; ENSMUSP00000029069; ENSMUSG00000027552.
GeneIDi13559.
KEGGimmu:13559.
UCSCiuc008oqk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86925 mRNA. Translation: CAA60508.1.
AK156760 mRNA. Translation: BAE33842.1.
BC003220 mRNA. Translation: AAH03220.1.
CCDSiCCDS38391.1.
PIRiI48338.
RefSeqiNP_031918.2. NM_007892.2.
UniGeneiMm.153415.

3D structure databases

ProteinModelPortaliQ61502.
SMRiQ61502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199352. 4 interactors.
IntActiQ61502. 3 interactors.
MINTiMINT-1784965.
STRINGi10090.ENSMUSP00000029069.

PTM databases

iPTMnetiQ61502.
PhosphoSitePlusiQ61502.

Proteomic databases

MaxQBiQ61502.
PaxDbiQ61502.
PeptideAtlasiQ61502.
PRIDEiQ61502.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029069; ENSMUSP00000029069; ENSMUSG00000027552.
GeneIDi13559.
KEGGimmu:13559.
UCSCiuc008oqk.1. mouse.

Organism-specific databases

CTDi1875.
MGIiMGI:105091. E2f5.

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiQ61502.
KOiK04682.

Enzyme and pathway databases

ReactomeiR-MMU-1538133. G0 and Early G1.
R-MMU-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-MMU-69231. Cyclin D associated events in G1.

Miscellaneous databases

ChiTaRSiE2f5. mouse.
PROiQ61502.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027552.
CleanExiMM_E2F5.
ExpressionAtlasiQ61502. baseline and differential.
GenevisibleiQ61502. MM.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE2F5_MOUSE
AccessioniPrimary (citable) accession number: Q61502
Secondary accession number(s): Q99LK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.