Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q61502 (E2F5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor E2F5

Short name=E2F-5
Gene names
Name:E2f5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction.

Subunit structure

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 By similarity.

Subcellular location

Nucleus.

Developmental stage

In the developing epidermis, first detected in 13.5-14.5 dpc embryos. With the appearance of stratified epithelium, levels of E2F5 expression increase and by 16.5 dpc, high expression found in the suprabasal cell layers. High expression also found in other regions with stratified squamous epithelia including the developing palate, lip and tongue. In the developing nervous system, first detected in the forebrain at 9.5 dpc. At 10.5 dpc, strongly expressed in the rostral region of the spinal cord. By 11.5 dpc, E2F5 is expressed throughout the developing central nervous system. In 12.5-15.5 dpc embryos, expression found in the undifferentiated ventricular regions of the brain. In the retina, expressed, in 14.5-18.5 dpc embryos, in the retinoblastic cell layer. In other developing tissues, highly expressed in the choroid plexus. Also found in the kidney, liver, lung, heart and weakly, in developing skeletal muscle and chondrocytes. Ref.4 Ref.5

Sequence similarities

Belongs to the E2F/DP family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Tfdp2Q64163-42EBI-7225685,EBI-8077763

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Transcription factor E2F5
PRO_0000219470

Regions

DNA binding37 – 10872 Potential
Region66 – 8823Leucine-zipper
Region109 – 20597Dimerization Potential
Region277 – 33559Transactivation Potential
Region312 – 32918RBL2 association Potential
Motif71 – 10838DEF box
Compositional bias223 – 2264Poly-Ser

Experimental info

Sequence conflict32 – 354AALA → RRSR in CAA60508. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61502 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 7922ACE79C73944B

FASTA33536,555
        10         20         30         40         50         60 
MAAAEPTSSA QPTPQAQAQP PPHGAPSSQP SAALAGGSSR HEKSLGLLTT KFVSLLQEAQ 

        70         80         90        100        110        120 
DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN SIQWKGVGAG CNTKEVIDRL 

       130        140        150        160        170        180 
RCLKAEIEDL ELKERELDQQ KLWLQQSIKN VMEDSINNRF SYVTHEDICN CFHGDTLLAI 

       190        200        210        220        230        240 
QAPSGTQLEV PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT 

       250        260        270        280        290        300 
QPSSQSSTSV TPQKSTMAAQ NLPEQHVSER SQTFQQTPAA EVSSGSISGD IIDELMSSDV 

       310        320        330 
FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and functional characterisation of E2F-5, a new member of the E2F family."
Buck V., Allen K.E., Soerensen T., Bybee A., Hijmans E.M., Voorhoeve P.M., Bernards R., la Thangue N.B.
Oncogene 11:31-38(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Spleen.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Expression patterns of the E2F family of transcription factors during mouse nervous system development."
Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"Expression patterns of the E2F family of transcription factors during murine epithelial development."
Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86925 mRNA. Translation: CAA60508.1.
AK156760 mRNA. Translation: BAE33842.1.
BC003220 mRNA. Translation: AAH03220.1.
CCDSCCDS38391.1.
PIRI48338.
RefSeqNP_031918.2. NM_007892.2.
UniGeneMm.153415.

3D structure databases

ProteinModelPortalQ61502.
SMRQ61502. Positions 39-105.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199352. 2 interactions.
IntActQ61502. 3 interactions.
MINTMINT-1784965.
STRING10090.ENSMUSP00000029069.

PTM databases

PhosphoSiteQ61502.

Proteomic databases

PRIDEQ61502.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029069; ENSMUSP00000029069; ENSMUSG00000027552.
GeneID13559.
KEGGmmu:13559.
UCSCuc008oqk.1. mouse.

Organism-specific databases

CTD1875.
MGIMGI:105091. E2f5.

Phylogenomic databases

eggNOGNOG289227.
GeneTreeENSGT00550000074403.
HOGENOMHOG000232045.
HOVERGENHBG002227.
InParanoidQ99LK0.
KOK04682.
OrthoDBEOG7WHHB1.

Gene expression databases

ArrayExpressQ61502.
BgeeQ61502.
CleanExMM_E2F5.
GenevestigatorQ61502.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR015633. E2F.
IPR028316. E2F5.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR12081. PTHR12081. 1 hit.
PTHR12081:SF35. PTHR12081:SF35. 1 hit.
PfamPF02319. E2F_TDP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284178.
PROQ61502.
SOURCESearch...

Entry information

Entry nameE2F5_MOUSE
AccessionPrimary (citable) accession number: Q61502
Secondary accession number(s): Q99LK0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot