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Q61502

- E2F5_MOUSE

UniProt

Q61502 - E2F5_MOUSE

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Protein

Transcription factor E2F5

Gene

E2f5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi37 – 10872Sequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: MGI
  2. organ morphogenesis Source: MGI
  3. regulation of cell cycle Source: MGI
  4. regulation of transcription, DNA-templated Source: MGI
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cilium biogenesis/degradation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_199110. G0 and Early G1.
REACT_203903. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_258573. Cyclin D associated events in G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F5
Short name:
E2F-5
Gene namesi
Name:E2f5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:105091. E2f5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nuclear envelope Source: MGI
  3. nucleus Source: MGI
  4. transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Transcription factor E2F5PRO_0000219470Add
BLAST

Proteomic databases

MaxQBiQ61502.
PRIDEiQ61502.

PTM databases

PhosphoSiteiQ61502.

Expressioni

Developmental stagei

In the developing epidermis, first detected in 13.5-14.5 dpc embryos. With the appearance of stratified epithelium, levels of E2F5 expression increase and by 16.5 dpc, high expression found in the suprabasal cell layers. High expression also found in other regions with stratified squamous epithelia including the developing palate, lip and tongue. In the developing nervous system, first detected in the forebrain at 9.5 dpc. At 10.5 dpc, strongly expressed in the rostral region of the spinal cord. By 11.5 dpc, E2F5 is expressed throughout the developing central nervous system. In 12.5-15.5 dpc embryos, expression found in the undifferentiated ventricular regions of the brain. In the retina, expressed, in 14.5-18.5 dpc embryos, in the retinoblastic cell layer. In other developing tissues, highly expressed in the choroid plexus. Also found in the kidney, liver, lung, heart and weakly, in developing skeletal muscle and chondrocytes.2 Publications

Gene expression databases

BgeeiQ61502.
CleanExiMM_E2F5.
ExpressionAtlasiQ61502. baseline and differential.
GenevestigatoriQ61502.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tfdp2Q64163-42EBI-7225685,EBI-8077763

Protein-protein interaction databases

BioGridi199352. 2 interactions.
IntActiQ61502. 3 interactions.
MINTiMINT-1784965.
STRINGi10090.ENSMUSP00000029069.

Structurei

3D structure databases

ProteinModelPortaliQ61502.
SMRiQ61502. Positions 39-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni66 – 8823Leucine-zipperAdd
BLAST
Regioni109 – 20597DimerizationSequence AnalysisAdd
BLAST
Regioni277 – 33559TransactivationSequence AnalysisAdd
BLAST
Regioni312 – 32918RBL2 associationSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 10838DEF boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi223 – 2264Poly-Ser

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiNOG289227.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiQ61502.
KOiK04682.
OrthoDBiEOG7WHHB1.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR028316. E2F5.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PTHR12081:SF35. PTHR12081:SF35. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61502-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAEPTSSA QPTPQAQAQP PPHGAPSSQP SAALAGGSSR HEKSLGLLTT
60 70 80 90 100
KFVSLLQEAQ DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN
110 120 130 140 150
SIQWKGVGAG CNTKEVIDRL RCLKAEIEDL ELKERELDQQ KLWLQQSIKN
160 170 180 190 200
VMEDSINNRF SYVTHEDICN CFHGDTLLAI QAPSGTQLEV PIPEMGQNGQ
210 220 230 240 250
KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT QPSSQSSTSV
260 270 280 290 300
TPQKSTMAAQ NLPEQHVSER SQTFQQTPAA EVSSGSISGD IIDELMSSDV
310 320 330
FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY
Length:335
Mass (Da):36,555
Last modified:July 27, 2011 - v2
Checksum:i7922ACE79C73944B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 354AALA → RRSR in CAA60508. (PubMed:7542760)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86925 mRNA. Translation: CAA60508.1.
AK156760 mRNA. Translation: BAE33842.1.
BC003220 mRNA. Translation: AAH03220.1.
CCDSiCCDS38391.1.
PIRiI48338.
RefSeqiNP_031918.2. NM_007892.2.
UniGeneiMm.153415.

Genome annotation databases

EnsembliENSMUST00000029069; ENSMUSP00000029069; ENSMUSG00000027552.
GeneIDi13559.
KEGGimmu:13559.
UCSCiuc008oqk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86925 mRNA. Translation: CAA60508.1 .
AK156760 mRNA. Translation: BAE33842.1 .
BC003220 mRNA. Translation: AAH03220.1 .
CCDSi CCDS38391.1.
PIRi I48338.
RefSeqi NP_031918.2. NM_007892.2.
UniGenei Mm.153415.

3D structure databases

ProteinModelPortali Q61502.
SMRi Q61502. Positions 39-105.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199352. 2 interactions.
IntActi Q61502. 3 interactions.
MINTi MINT-1784965.
STRINGi 10090.ENSMUSP00000029069.

PTM databases

PhosphoSitei Q61502.

Proteomic databases

MaxQBi Q61502.
PRIDEi Q61502.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029069 ; ENSMUSP00000029069 ; ENSMUSG00000027552 .
GeneIDi 13559.
KEGGi mmu:13559.
UCSCi uc008oqk.1. mouse.

Organism-specific databases

CTDi 1875.
MGIi MGI:105091. E2f5.

Phylogenomic databases

eggNOGi NOG289227.
GeneTreei ENSGT00550000074403.
HOGENOMi HOG000232045.
HOVERGENi HBG002227.
InParanoidi Q61502.
KOi K04682.
OrthoDBi EOG7WHHB1.

Enzyme and pathway databases

Reactomei REACT_199110. G0 and Early G1.
REACT_203903. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_258573. Cyclin D associated events in G1.

Miscellaneous databases

ChiTaRSi E2f5. mouse.
NextBioi 284178.
PROi Q61502.
SOURCEi Search...

Gene expression databases

Bgeei Q61502.
CleanExi MM_E2F5.
ExpressionAtlasi Q61502. baseline and differential.
Genevestigatori Q61502.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR015633. E2F.
IPR028316. E2F5.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR12081. PTHR12081. 1 hit.
PTHR12081:SF35. PTHR12081:SF35. 1 hit.
Pfami PF02319. E2F_TDP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and functional characterisation of E2F-5, a new member of the E2F family."
    Buck V., Allen K.E., Soerensen T., Bybee A., Hijmans E.M., Voorhoeve P.M., Bernards R., la Thangue N.B.
    Oncogene 11:31-38(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Spleen.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Expression patterns of the E2F family of transcription factors during murine epithelial development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiE2F5_MOUSE
AccessioniPrimary (citable) accession number: Q61502
Secondary accession number(s): Q99LK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3