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Q61501

- E2F1_MOUSE

UniProt

Q61501 - E2F1_MOUSE

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Protein

Transcription factor E2F1

Gene
E2f1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.1 Publication

Enzyme regulationi

BIRC2/c-IAP1 stimulates its transcriptional activity By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi105 – 18985 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. core promoter binding Source: Ensembl
  2. DNA binding Source: MGI
  3. protein binding Source: IntAct
  4. sequence-specific DNA binding Source: Ensembl
  5. sequence-specific DNA binding transcription factor activity Source: MGI
  6. transcription factor binding Source: UniProtKB

GO - Biological processi

  1. anoikis Source: MGI
  2. cellular response to fatty acid Source: Ensembl
  3. cellular response to hypoxia Source: Ensembl
  4. DNA damage checkpoint Source: UniProtKB
  5. forebrain development Source: MGI
  6. intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  7. intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
  8. lens fiber cell apoptotic process Source: MGI
  9. mRNA stabilization Source: Ensembl
  10. negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
  11. positive regulation of fibroblast proliferation Source: Ensembl
  12. positive regulation of transcription, DNA-templated Source: MGI
  13. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  14. regulation of cell cycle Source: MGI
  15. regulation of G1/S transition of mitotic cell cycle Source: Ensembl
  16. regulation of transcription, DNA-templated Source: MGI
  17. spermatogenesis Source: Ensembl
  18. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Apoptosis, Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198608. G1/S-Specific Transcription.
REACT_199110. G0 and Early G1.
REACT_206830. E2F mediated regulation of DNA replication.
REACT_208887. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
REACT_227891. G2 Phase.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F1
Short name:
E2F-1
Gene namesi
Name:E2f1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:101941. E2f1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleoplasm Source: Reactome
  3. nucleus Source: MGI
  4. Rb-E2F complex Source: Ensembl
  5. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430Transcription factor E2F1PRO_0000219462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei112 – 1121N6-acetyllysine By similarity
Modified residuei115 – 1151N6-acetyllysine By similarity
Modified residuei120 – 1201N6-acetyllysine By similarity
Modified residuei368 – 3681Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis By similarity.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ61501.

PTM databases

PhosphoSiteiQ61501.

Expressioni

Developmental stagei

In the developing nervous system, first detected in the neural tube at day 9.5 dpc. By day 10.5, levels increase throughout the brain, with highest levels in the hindbrain and in the spinal cord, expressed only in the rostral half. By day 11.5, expression found throughout the brain and spinal cord. From day 12.5, expression restricted to the ventricular regions of the brain, peaks at day 13.5 and declines thereafter. Only weak expression in the developing spinal cord from day 11.5-16.5. In the developing retina, expression is confined to the undifferentiated retinoblastic cell layer. In other developing tissues, E2F1 is expressed in kidney, lung, liver hepatocytes, heart and thymus. Highest levels in liver. Absent in choroid plexus.1 Publication

Gene expression databases

ArrayExpressiQ61501.
BgeeiQ61501.
CleanExiMM_E2F1.
GenevestigatoriQ61501.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1. Interacts with ARID3A. Interacts with TRIM28; the interaction represses the transcriptional activity through recruiting HDAC1 which deacetylates E2F1. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity By similarity. Binds EAPP. Interacts with BIRC2/c-IAP1 (via BIR domains) By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NdnP252335EBI-1025536,EBI-1801080
Ndnl2Q9CPR85EBI-1025536,EBI-5529102

Protein-protein interaction databases

BioGridi199350. 17 interactions.
IntActiQ61501. 6 interactions.
MINTiMINT-4302111.

Structurei

3D structure databases

ProteinModelPortaliQ61501.
SMRiQ61501. Positions 121-185, 197-296.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 10342Cyclin A/CDK2 binding Reviewed predictionAdd
BLAST
Regioni84 – 186103Interaction with BIRC2/c-IAP1 By similarityAdd
BLAST
Regioni148 – 16922Leucine-zipperAdd
BLAST
Regioni190 – 27990Dimerization Reviewed predictionAdd
BLAST
Regioni361 – 43070Transactivation Reviewed predictionAdd
BLAST
Regioni402 – 41918Retinoblastoma protein RB1 binding Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi153 – 18937DEF boxAdd
BLAST

Sequence similaritiesi

Belongs to the E2F/DP family.

Phylogenomic databases

eggNOGiNOG328718.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiQ61501.
KOiK17454.
OMAiRHPGKGV.
OrthoDBiEOG738058.
PhylomeDBiQ61501.
TreeFamiTF105566.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61501-1 [UniParc]FASTAAdd to Basket

« Hide

MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP    50
TGPRDSDVLL FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS 100
GPFRGRGRHP GKGVKSPGEK SRYETSLNLT TKRFLELLSR SADGVVDLNW 150
AAEVLKVQKR RIYDITNVLE GIQLIAKKSK NHIQWLGSHT MVGIGKRLEG 200
LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY VTCQDLRSIA 250
DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG 300
ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA 350
VLPRMGHLRV PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE 400
ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF 430
Length:430
Mass (Da):46,323
Last modified:November 1, 1996 - v1
Checksum:iC5DF18AD3B4DFEFA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21973 mRNA. Translation: AAA83217.1.
BC052160 mRNA. Translation: AAH52160.2.
CCDSiCCDS16935.1.
PIRiA56209.
RefSeqiNP_031917.1. NM_007891.5.
UniGeneiMm.18036.
Mm.441323.

Genome annotation databases

EnsembliENSMUST00000103145; ENSMUSP00000099434; ENSMUSG00000027490.
GeneIDi13555.
KEGGimmu:13555.
UCSCiuc008njk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21973 mRNA. Translation: AAA83217.1 .
BC052160 mRNA. Translation: AAH52160.2 .
CCDSi CCDS16935.1.
PIRi A56209.
RefSeqi NP_031917.1. NM_007891.5.
UniGenei Mm.18036.
Mm.441323.

3D structure databases

ProteinModelPortali Q61501.
SMRi Q61501. Positions 121-185, 197-296.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199350. 17 interactions.
IntActi Q61501. 6 interactions.
MINTi MINT-4302111.

PTM databases

PhosphoSitei Q61501.

Proteomic databases

PRIDEi Q61501.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000103145 ; ENSMUSP00000099434 ; ENSMUSG00000027490 .
GeneIDi 13555.
KEGGi mmu:13555.
UCSCi uc008njk.1. mouse.

Organism-specific databases

CTDi 1869.
MGIi MGI:101941. E2f1.

Phylogenomic databases

eggNOGi NOG328718.
GeneTreei ENSGT00550000074403.
HOGENOMi HOG000232045.
HOVERGENi HBG002227.
InParanoidi Q61501.
KOi K17454.
OMAi RHPGKGV.
OrthoDBi EOG738058.
PhylomeDBi Q61501.
TreeFami TF105566.

Enzyme and pathway databases

Reactomei REACT_198608. G1/S-Specific Transcription.
REACT_199110. G0 and Early G1.
REACT_206830. E2F mediated regulation of DNA replication.
REACT_208887. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
REACT_227891. G2 Phase.

Miscellaneous databases

NextBioi 284170.
PROi Q61501.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61501.
Bgeei Q61501.
CleanExi MM_E2F1.
Genevestigatori Q61501.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR12081. PTHR12081. 1 hit.
Pfami PF02319. E2F_TDP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, chromosomal location, and characterization of mouse E2F1."
    Li Y., Slansky J.E., Myers D.J., Drinkwater N.R., Kaelin W.G. Jr., Farnham P.J.
    Mol. Cell. Biol. 14:1861-1869(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss albino.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  3. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. "E2F-1-induced p53-independent apoptosis in transgenic mice."
    Holmberg C., Helin K., Sehested M., Karlstroem O.
    Oncogene 17:143-155(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN APOPTOSIS.
  5. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
    Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
    Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EAPP.

Entry informationi

Entry nameiE2F1_MOUSE
AccessioniPrimary (citable) accession number: Q61501
Secondary accession number(s): Q80VZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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