Reviewed, UniProtKB/Swiss-Prot Q61501 (E2F1_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcription factor E2F1
      Short name=E2F-1

Gene names

Name:

E2f1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. Ref.4
Subunit structure	Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1. Interacts with ARID3A By similarity. Binds EAPP.
Subcellular location	Nucleus.
Developmental stage	In the developing nervous system, first detected in the neural tube at day 9.5 dpc. By day 10.5, levels increase throughout the brain, with highest levels in the hindbrain and in the spinal cord, expressed only in the rostral half. By day 11.5, expression found throughout the brain and spinal cord. From day 12.5, expression restricted to the ventricular regions of the brain, peaks at day 13.5 and declines thereafter. Only weak expression in the developing spinal cord from day 11.5-16.5. In the developing retina, expression is confined to the undifferentiated retinoblastic cell layer. In other developing tissues, E2F-1 is expressed in kidney, lung, liver hepatocytes, heart and thymus. Highest levels in liver. Absent in choroid plexus. Ref.3
Post-translational modification	Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase By similarity.
Sequence similarities	Belongs to the E2F/DP family.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	apoptosis Inferred from direct assay. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW forebrain development Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI regulation of cell cycle Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: MGI transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	transcription activator activity Inferred from direct assay. Source: MGI transcription factor activity Inferred from direct assay. Source: MGI transcription factor binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Parp1	P11103	1	EBI-1025536,EBI-642213
Sp1	O89090	1	EBI-1025536,EBI-645192

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Transcription factor E2F1		PRO_0000219462
Regions
	Domain	148 – 169	22	Leucine-zipper
	DNA binding	105 – 189	85	Potential
	Region	62 – 103	42	Cyclin A/CDK2 binding Potential
	Region	190 – 279	90	Dimerization Potential
	Region	361 – 430	70	Transactivation Potential
	Region	402 – 419	18	Retinoblastoma protein RB1 binding Potential
	Motif	153 – 189	37	DEF box
Amino acid modifications
	Modified residue	368	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q61501-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C5DF18AD3B4DFEFA	FASTA	430	46,323
	10 20 30 40 50 60 MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP TGPRDSDVLL 70 80 90 100 110 120 FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS GPFRGRGRHP GKGVKSPGEK 130 140 150 160 170 180 SRYETSLNLT TKRFLELLSR SADGVVDLNW AAEVLKVQKR RIYDITNVLE GIQLIAKKSK 190 200 210 220 230 240 NHIQWLGSHT MVGIGKRLEG LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY 250 260 270 280 290 300 VTCQDLRSIA DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG 310 320 330 340 350 360 ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA VLPRMGHLRV 370 380 390 400 410 420 PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE ALDYHFGLEE GEGIRDLFDC 430 DFGDLTPLDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, chromosomal location, and characterization of mouse E2F1." Li Y., Slansky J.E., Myers D.J., Drinkwater N.R., Kaelin W.G. Jr., Farnham P.J. Mol. Cell. Biol. 14:1861-1869(1994) [PubMed: 8114719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss albino.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"Expression patterns of the E2F family of transcription factors during mouse nervous system development." Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A. Mech. Dev. 66:13-25(1997) [PubMed: 9376316] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[4]	"E2F-1-induced p53-independent apoptosis in transgenic mice." Holmberg C., Helin K., Sehested M., Karlstroem O. Oncogene 17:143-155(1998) [PubMed: 9674698] [Abstract] Cited for: FUNCTION IN APOPTOSIS.
[5]	"EAPP, a novel E2F binding protein that modulates E2F-dependent transcription." Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H. Mol. Biol. Cell 16:2181-2190(2005) [PubMed: 15716352] [Abstract] Cited for: INTERACTION WITH EAPP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L21973 mRNA. Translation: AAA83217.1. BC052160 mRNA. Translation: AAH52160.2.
IPI	IPI00338528.
PIR	A56209.
RefSeq	NP_031917.1.
UniGene	Mm.18036
3D structure databases
HSSP	HSSP built from PDB template 1CF7 based on UniProtKB Q16254.
SMR	Q61501. Positions 121-185, 197-296.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61501. 4 interactions.
PTM databases
PhosphoSite	Q61501.
Genome annotation databases
Ensembl	ENSMUSG00000027490. Mus musculus. [Contig view]
GeneID	13555.
KEGG	mmu:13555.
Organism-specific databases
MGI	MGI:101941. E2f1.
Phylogenomic databases
HOGENOM	Q61501.
HOVERGEN	Q61501.
OMA	Q61501. QWLGSHT.
Gene expression databases
ArrayExpress	Q61501.
Bgee	Q61501.
CleanEx	MM_E2F1.
GermOnline	ENSMUSG00000027490. Mus musculus.
Family and domain databases
InterPro	IPR015633. E2F. IPR015634. E2F1. IPR003316. E2F_TDP. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR12081. E2F. 1 hit. PTHR12081:SF13. E2F1. 1 hit.
Pfam	PF02319. E2F_TDP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	284170.
SOURCE	Search...

Entry information

Entry name

E2F1_MOUSE

Accession

Primary (citable) accession number: Q61501
Secondary accession number(s): Q80VZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)