Q61501 (E2F1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor E2F1
Short name=E2F-1

Gene names

Name:

E2f1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. Ref.4
Subunit structure	Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1. Interacts with ARID3A. Interacts with TRIM28; the interaction represses the transcriptional activity through recruiting HDAC1 which deacetylates E2F1. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity By similarity. Binds EAPP. Ref.5
Subcellular location	Nucleus.
Developmental stage	In the developing nervous system, first detected in the neural tube at day 9.5 dpc. By day 10.5, levels increase throughout the brain, with highest levels in the hindbrain and in the spinal cord, expressed only in the rostral half. By day 11.5, expression found throughout the brain and spinal cord. From day 12.5, expression restricted to the ventricular regions of the brain, peaks at day 13.5 and declines thereafter. Only weak expression in the developing spinal cord from day 11.5-16.5. In the developing retina, expression is confined to the undifferentiated retinoblastic cell layer. In other developing tissues, E2F-1 is expressed in kidney, lung, liver hepatocytes, heart and thymus. Highest levels in liver. Absent in choroid plexus. Ref.3
Post-translational modification	Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis By similarity. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B By similarity.
Sequence similarities	Belongs to the E2F/DP family.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA damage checkpoint Inferred from sequence or structural similarity. Source: UniProtKB apoptotic process Inferred from direct assay. Source: MGI forebrain development Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: MGI transcription factor binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Transcription factor E2F1		PRO_0000219462
Regions
	Domain	148 – 169	22	Leucine-zipper
	DNA binding	105 – 189	85	Potential
	Region	62 – 103	42	Cyclin A/CDK2 binding Potential
	Region	190 – 279	90	Dimerization Potential
	Region	361 – 430	70	Transactivation Potential
	Region	402 – 419	18	Retinoblastoma protein RB1 binding Potential
	Motif	153 – 189	37	DEF box
Amino acid modifications
	Modified residue	112	1	N6-acetyllysine By similarity
	Modified residue	115	1	N6-acetyllysine By similarity
	Modified residue	120	1	N6-acetyllysine By similarity
	Modified residue	368	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q61501 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C5DF18AD3B4DFEFA	FASTA	430	46,323
	10 20 30 40 50 60 MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP TGPRDSDVLL 70 80 90 100 110 120 FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS GPFRGRGRHP GKGVKSPGEK 130 140 150 160 170 180 SRYETSLNLT TKRFLELLSR SADGVVDLNW AAEVLKVQKR RIYDITNVLE GIQLIAKKSK 190 200 210 220 230 240 NHIQWLGSHT MVGIGKRLEG LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY 250 260 270 280 290 300 VTCQDLRSIA DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG 310 320 330 340 350 360 ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA VLPRMGHLRV 370 380 390 400 410 420 PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE ALDYHFGLEE GEGIRDLFDC 430 DFGDLTPLDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, chromosomal location, and characterization of mouse E2F1." Li Y., Slansky J.E., Myers D.J., Drinkwater N.R., Kaelin W.G. Jr., Farnham P.J. Mol. Cell. Biol. 14:1861-1869(1994) [PubMed: 8114719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss albino.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"Expression patterns of the E2F family of transcription factors during mouse nervous system development." Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A. Mech. Dev. 66:13-25(1997) [PubMed: 9376316] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[4]	"E2F-1-induced p53-independent apoptosis in transgenic mice." Holmberg C., Helin K., Sehested M., Karlstroem O. Oncogene 17:143-155(1998) [PubMed: 9674698] [Abstract] Cited for: FUNCTION IN APOPTOSIS.
[5]	"EAPP, a novel E2F binding protein that modulates E2F-dependent transcription." Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H. Mol. Biol. Cell 16:2181-2190(2005) [PubMed: 15716352] [Abstract] Cited for: INTERACTION WITH EAPP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L21973 mRNA. Translation: AAA83217.1. BC052160 mRNA. Translation: AAH52160.2.
IPI	IPI00338528.
PIR	A56209.
RefSeq	NP_031917.1. NM_007891.4.
UniGene	Mm.18036.
3D structure databases
ProteinModelPortal	Q61501.
SMR	Q61501. Positions 121-185, 197-296.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61501. 3 interactions.
MINT	MINT-4302111.
STRING	Q61501.
PTM databases
PhosphoSite	Q61501.
Proteomic databases
PRIDE	Q61501.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103145; ENSMUSP00000099434; ENSMUSG00000027490.
GeneID	13555.
KEGG	mmu:13555.
Organism-specific databases
CTD	1869.
MGI	MGI:101941. E2f1.
Phylogenomic databases
GeneTree	ENSGT00550000074403.
HOGENOM	HBG715983.
HOVERGEN	HBG002227.
InParanoid	Q61501.
OMA	ICTTQLR.
OrthoDB	EOG42821X.
Enzyme and pathway databases
Reactome	REACT_111209. Transcription of E2f targets under negative control by p107 (Rbl1) and p130 (Rbl2) in complex with Hdac1.
Gene expression databases
ArrayExpress	Q61501.
Bgee	Q61501.
CleanEx	MM_E2F1.
Genevestigator	Q61501.
GermOnline	ENSMUSG00000027490. Mus musculus.
Family and domain databases
InterPro	IPR015633. E2F. IPR003316. E2F_TDP. IPR015634. Transcription_factor_E2F1. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K06620.
PANTHER	PTHR12081. E2F. 1 hit. PTHR12081:SF13. E2F1. 1 hit.
Pfam	PF02319. E2F_TDP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	284170.
SOURCE	Search...

Entry information

Entry name

E2F1_MOUSE

Accession

Primary (citable) accession number: Q61501
Secondary accession number(s): Q80VZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program