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Q61501

- E2F1_MOUSE

UniProt

Q61501 - E2F1_MOUSE

Protein

Transcription factor E2F1

Gene

E2f1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.1 Publication

    Enzyme regulationi

    BIRC2/c-IAP1 stimulates its transcriptional activity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi105 – 18985Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. core promoter binding Source: Ensembl
    2. DNA binding Source: MGI
    3. protein binding Source: IntAct
    4. sequence-specific DNA binding Source: Ensembl
    5. sequence-specific DNA binding transcription factor activity Source: MGI
    6. transcription factor binding Source: UniProtKB

    GO - Biological processi

    1. anoikis Source: MGI
    2. cellular response to fatty acid Source: Ensembl
    3. cellular response to hypoxia Source: Ensembl
    4. DNA damage checkpoint Source: UniProtKB
    5. forebrain development Source: MGI
    6. intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
    7. intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
    8. lens fiber cell apoptotic process Source: MGI
    9. mRNA stabilization Source: Ensembl
    10. negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
    11. positive regulation of fibroblast proliferation Source: Ensembl
    12. positive regulation of transcription, DNA-templated Source: MGI
    13. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    14. regulation of cell cycle Source: MGI
    15. regulation of G1/S transition of mitotic cell cycle Source: Ensembl
    16. regulation of transcription, DNA-templated Source: MGI
    17. spermatogenesis Source: Ensembl
    18. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Apoptosis, Cell cycle, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198608. G1/S-Specific Transcription.
    REACT_199110. G0 and Early G1.
    REACT_206830. E2F mediated regulation of DNA replication.
    REACT_208887. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
    REACT_227891. G2 Phase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor E2F1
    Short name:
    E2F-1
    Gene namesi
    Name:E2f1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:101941. E2f1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleoplasm Source: Reactome
    3. nucleus Source: MGI
    4. Rb-E2F complex Source: Ensembl
    5. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 430430Transcription factor E2F1PRO_0000219462Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei112 – 1121N6-acetyllysineBy similarity
    Modified residuei115 – 1151N6-acetyllysineBy similarity
    Modified residuei120 – 1201N6-acetyllysineBy similarity
    Modified residuei368 – 3681PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis By similarity.By similarity
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ61501.

    PTM databases

    PhosphoSiteiQ61501.

    Expressioni

    Developmental stagei

    In the developing nervous system, first detected in the neural tube at day 9.5 dpc. By day 10.5, levels increase throughout the brain, with highest levels in the hindbrain and in the spinal cord, expressed only in the rostral half. By day 11.5, expression found throughout the brain and spinal cord. From day 12.5, expression restricted to the ventricular regions of the brain, peaks at day 13.5 and declines thereafter. Only weak expression in the developing spinal cord from day 11.5-16.5. In the developing retina, expression is confined to the undifferentiated retinoblastic cell layer. In other developing tissues, E2F1 is expressed in kidney, lung, liver hepatocytes, heart and thymus. Highest levels in liver. Absent in choroid plexus.1 Publication

    Gene expression databases

    ArrayExpressiQ61501.
    BgeeiQ61501.
    CleanExiMM_E2F1.
    GenevestigatoriQ61501.

    Interactioni

    Subunit structurei

    Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1. Interacts with ARID3A. Interacts with TRIM28; the interaction represses the transcriptional activity through recruiting HDAC1 which deacetylates E2F1. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity By similarity. Binds EAPP. Interacts with BIRC2/c-IAP1 (via BIR domains) By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NdnP252335EBI-1025536,EBI-1801080
    Ndnl2Q9CPR85EBI-1025536,EBI-5529102

    Protein-protein interaction databases

    BioGridi199350. 17 interactions.
    IntActiQ61501. 6 interactions.
    MINTiMINT-4302111.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61501.
    SMRiQ61501. Positions 121-185, 197-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni62 – 10342Cyclin A/CDK2 bindingSequence AnalysisAdd
    BLAST
    Regioni84 – 186103Interaction with BIRC2/c-IAP1By similarityAdd
    BLAST
    Regioni148 – 16922Leucine-zipperAdd
    BLAST
    Regioni190 – 27990DimerizationSequence AnalysisAdd
    BLAST
    Regioni361 – 43070TransactivationSequence AnalysisAdd
    BLAST
    Regioni402 – 41918Retinoblastoma protein RB1 bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi153 – 18937DEF boxAdd
    BLAST

    Sequence similaritiesi

    Belongs to the E2F/DP family.Curated

    Phylogenomic databases

    eggNOGiNOG328718.
    GeneTreeiENSGT00550000074403.
    HOGENOMiHOG000232045.
    HOVERGENiHBG002227.
    InParanoidiQ61501.
    KOiK17454.
    OMAiRHPGKGV.
    OrthoDBiEOG738058.
    PhylomeDBiQ61501.
    TreeFamiTF105566.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR12081. PTHR12081. 1 hit.
    PfamiPF02319. E2F_TDP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61501-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQLPAAPP    50
    TGPRDSDVLL FATPQAPRPA PSAPRPALGR PPVKRRLDLE TDHQYLAGSS 100
    GPFRGRGRHP GKGVKSPGEK SRYETSLNLT TKRFLELLSR SADGVVDLNW 150
    AAEVLKVQKR RIYDITNVLE GIQLIAKKSK NHIQWLGSHT MVGIGKRLEG 200
    LTQDLQQLQE SEQQLDHLMH ICTTQLQLLS EDSDTQRLAY VTCQDLRSIA 250
    DPAEQMVIVI KAPPETQLQA VDSSETFQIS LKSKQGPIDV FLCPEESADG 300
    ISPGKTSCQE TSSGEDRTAD SGPAGPPPSP PSTSPALDPS QSLLGLEQEA 350
    VLPRMGHLRV PMEEDQLSPL VAADSLLEHV KEDFSGLLPG EFISLSPPHE 400
    ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF 430
    Length:430
    Mass (Da):46,323
    Last modified:November 1, 1996 - v1
    Checksum:iC5DF18AD3B4DFEFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21973 mRNA. Translation: AAA83217.1.
    BC052160 mRNA. Translation: AAH52160.2.
    CCDSiCCDS16935.1.
    PIRiA56209.
    RefSeqiNP_031917.1. NM_007891.5.
    UniGeneiMm.18036.
    Mm.441323.

    Genome annotation databases

    EnsembliENSMUST00000103145; ENSMUSP00000099434; ENSMUSG00000027490.
    GeneIDi13555.
    KEGGimmu:13555.
    UCSCiuc008njk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21973 mRNA. Translation: AAA83217.1 .
    BC052160 mRNA. Translation: AAH52160.2 .
    CCDSi CCDS16935.1.
    PIRi A56209.
    RefSeqi NP_031917.1. NM_007891.5.
    UniGenei Mm.18036.
    Mm.441323.

    3D structure databases

    ProteinModelPortali Q61501.
    SMRi Q61501. Positions 121-185, 197-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199350. 17 interactions.
    IntActi Q61501. 6 interactions.
    MINTi MINT-4302111.

    PTM databases

    PhosphoSitei Q61501.

    Proteomic databases

    PRIDEi Q61501.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103145 ; ENSMUSP00000099434 ; ENSMUSG00000027490 .
    GeneIDi 13555.
    KEGGi mmu:13555.
    UCSCi uc008njk.1. mouse.

    Organism-specific databases

    CTDi 1869.
    MGIi MGI:101941. E2f1.

    Phylogenomic databases

    eggNOGi NOG328718.
    GeneTreei ENSGT00550000074403.
    HOGENOMi HOG000232045.
    HOVERGENi HBG002227.
    InParanoidi Q61501.
    KOi K17454.
    OMAi RHPGKGV.
    OrthoDBi EOG738058.
    PhylomeDBi Q61501.
    TreeFami TF105566.

    Enzyme and pathway databases

    Reactomei REACT_198608. G1/S-Specific Transcription.
    REACT_199110. G0 and Early G1.
    REACT_206830. E2F mediated regulation of DNA replication.
    REACT_208887. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
    REACT_227891. G2 Phase.

    Miscellaneous databases

    NextBioi 284170.
    PROi Q61501.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61501.
    Bgeei Q61501.
    CleanExi MM_E2F1.
    Genevestigatori Q61501.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR12081. PTHR12081. 1 hit.
    Pfami PF02319. E2F_TDP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, chromosomal location, and characterization of mouse E2F1."
      Li Y., Slansky J.E., Myers D.J., Drinkwater N.R., Kaelin W.G. Jr., Farnham P.J.
      Mol. Cell. Biol. 14:1861-1869(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss albino.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.
    3. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
      Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
      Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    4. "E2F-1-induced p53-independent apoptosis in transgenic mice."
      Holmberg C., Helin K., Sehested M., Karlstroem O.
      Oncogene 17:143-155(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN APOPTOSIS.
    5. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
      Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
      Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EAPP.

    Entry informationi

    Entry nameiE2F1_MOUSE
    AccessioniPrimary (citable) accession number: Q61501
    Secondary accession number(s): Q80VZ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3