Q61496 (DDX4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent RNA helicase DDX4
EC=3.6.4.13

Alternative name(s):
DEAD box protein 4
Mvh
Vasa homolog

Gene names

Name:	Ddx4
Synonyms:	Vasa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	702 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in germ cell development. May play a role in sperm motility By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Ref.3 Ref.4 Ref.5 Ref.6
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region.
Tissue specificity	Testis.
Developmental stage	Expressed in spermatogenic cells from the spermatocyte stage to the round spermatid stage.
Sequence similarities	Belongs to the DEAD box helicase family. DDX4/VASA subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	male meiosis I Inferred from mutant phenotype PubMed 10766740. Source: MGI multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of protein localization Inferred from mutant phenotype Ref.6. Source: MGI spermatogenesis Inferred from mutant phenotype PubMed 10766740. Source: MGI
Cellular_component	chromatoid body Inferred from direct assay PubMed 10781947PubMed 22792342. Source: MGI nucleus Inferred from direct assay PubMed 12798292. Source: MGI perinuclear region of cytoplasm Inferred from direct assay PubMed 10781947PubMed 21991325. Source: MGI pi-body Inferred from direct assay PubMed 20011505. Source: UniProtKB piP-body Inferred from direct assay PubMed 20011505. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 702	702	Probable ATP-dependent RNA helicase DDX4		PRO_0000054979
Regions
	Domain	292 – 475	184	Helicase ATP-binding
	Domain	503 – 648	146	Helicase C-terminal
	Nucleotide binding	305 – 312	8	ATP By similarity
	Motif	261 – 289	29	Q motif
	Motif	419 – 422	4	DEAD box
	Compositional bias	58 – 207	150	Gly-rich
Experimental info
	Sequence conflict	152	1	R → C in BAA03584. Ref.2
	Sequence conflict	156 – 160	5	LFGSR → FLVLG in BAA03584. Ref.2
	Sequence conflict	277	1	A → R in BAA03584. Ref.2
	Sequence conflict	291	1	S → T in BAA03584. Ref.2
	Sequence conflict	368 – 369	2	RA → IS in BAA03584. Ref.2
	Sequence conflict	423	1	R → S in BAA03584. Ref.2
	Sequence conflict	430	1	G → A in BAA03584. Ref.2
	Sequence conflict	433	1	M → I in BAA03584. Ref.2
	Sequence conflict	448	1	R → H in BAA03584. Ref.2
	Sequence conflict	472	1	S → N in BAA03584. Ref.2
	Sequence conflict	499 – 509	11	SKREKLVEILR → QKEKSLLRFYE in BAA03584. Ref.2
	Sequence conflict	540	1	T → S in BAA03584. Ref.2
	Sequence conflict	680 – 702	23	HTLNT…DESWD → AHVEYSGDFFFTSSQSS Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61496 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 5D1E4CC0BDF39748	FASTA	702	76,470
	10 20 30 40 50 60 MGDEDWEAEI LKPHVSSYVP VFEKDKYSSG ANGDTFNRTS ASSDIGESSK KENTSTTGGF 70 80 90 100 110 120 GRGKGFGNRG FLNNKFEEGD SSGFWKESNN DCEDNQTRSR GFSKRGGCQD GNDSEASGPF 130 140 150 160 170 180 RRGGRGSFRG CRGGFGLGRP NSESDQDQGT QRGGGLFGSR KPAASDSGNG DTYQSRSGSG 190 200 210 220 230 240 RGGYKGLNEE VVTGSGKNSW KSETEGGESS DSQGPKVTYI PPPPPEDEDS IFAHYQTGIN 250 260 270 280 290 300 FDKYDTILVE VSGHDAPPAI LTFEEANLCQ TLNNNIAKAG YTKLTPVQKY SIPIVLAGRD 310 320 330 340 350 360 LMACAQTGSG KTAAFLLPIL AHMMRDGITA SRFKELQEPE CIIVAPTREL INQIYLEARK 370 380 390 400 410 420 FSFGTCVRAV VIYGGTQFGH SVRQIVQGCN ILCATPGRLM DIIGKEKIGL KQVKYLVLDE 430 440 450 460 470 480 ADRMLDMGFG PEMKKLISCP GMPSKEQRQT LLFSATFPEE IQRLAGDFLK SSYLFVAVGQ 490 500 510 520 530 540 VGGACRDVQQ TILQVGQYSK REKLVEILRN IGDERTMVFV ETKKKADFIA TFLCQEKIST 550 560 570 580 590 600 TSIHGDREQR EREQALGDFR CGKCPVLVAT SVAARGLDIE NVQHVINFDL PSTIDEYVHR 610 620 630 640 650 660 IGRTGRCGNT GRAISFFDTD SDNHLAQPLV KVLSDAQQDV PAWLEEIAFS TYVPPSFSSS 670 680 690 700 TRGGAVFASV DTRKNYQGKH TLNTAGISSS QAPNPVDDES WD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[2]	"Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage." Fujiwara Y., Komiya T., Kawabata H., Sato M., Fujimoto H., Furusawa M., Noce T. Proc. Natl. Acad. Sci. U.S.A. 91:12258-12262(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-702. Strain: BALB/c. Tissue: Testis.
[3]	"Mili, a mammalian member of piwi family gene, is essential for spermatogenesis." Kuramochi-Miyagawa S., Kimura T., Ijiri T.W., Isobe T., Asada N., Fujita Y., Ikawa M., Iwai N., Okabe M., Deng W., Lin H., Matsuda Y., Nakano T. Development 131:839-849(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PIWIL2.
[4]	"Mouse RanBPM is a partner gene to a germline specific RNA helicase, mouse vasa homolog protein." Shibata N., Tsunekawa N., Okamoto-Ito S., Akasu R., Tokumasu A., Noce T. Mol. Reprod. Dev. 67:1-7(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RANBP9. Tissue: Testis.
[5]	"Mouse MAELSTROM: the link between meiotic silencing of unsynapsed chromatin and microRNA pathway?" Costa Y., Speed R.M., Gautier P., Semple C.A., Maratou K., Turner J.M.A., Cooke H.J. Hum. Mol. Genet. 15:2324-2334(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAEL.
[6]	"Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice." Hosokawa M., Shoji M., Kitamura K., Tanaka T., Noce T., Chuma S., Nakatsuji N. Dev. Biol. 301:38-52(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A MRNP COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK014844 mRNA. Translation: BAB29578.1. D14859 mRNA. Translation: BAA03584.1.
IPI	IPI00121394.
RefSeq	NP_034159.1. NM_010029.2.
UniGene	Mm.12818.
3D structure databases
ProteinModelPortal	Q61496.
SMR	Q61496. Positions 219-648.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000096769.
PTM databases
PhosphoSite	Q61496.
2D gel databases
REPRODUCTION-2DPAGE	IPI00121394. Q61496.
Proteomic databases
PaxDb	Q61496.
PRIDE	Q61496.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075748; ENSMUSP00000075157; ENSMUSG00000021758.
GeneID	13206.
KEGG	mmu:13206.
UCSC	uc007rwm.2. mouse.
Organism-specific databases
CTD	54514.
MGI	MGI:102670. Ddx4.
Phylogenomic databases
eggNOG	COG0513.
GeneTree	ENSGT00620000087942.
HOGENOM	HOG000268804.
HOVERGEN	HBG015893.
KO	K13982.
Gene expression databases
ArrayExpress	Q61496.
Bgee	Q61496.
CleanEx	MM_DDX4.
Genevestigator	Q61496.
GermOnline	ENSMUSG00000021758. Mus musculus.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DDX4. mouse.
NextBio	283368.
SOURCE	Search...

Entry information

Entry name

DDX4_MOUSE

Accession

Primary (citable) accession number: Q61496
Secondary accession number(s): Q9D5X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2002
Last modified:	May 29, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program