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Q61490 (CD166_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD166 antigen
Alternative name(s):
Activated leukocyte cell adhesion molecule
Protein DM-GRASP
CD_antigen=CD166
Gene names
Name:Alcam
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed at high levels in the brain, and lung, and at lower levels in the liver, and the kidney, as well as by activated leukocytes and a number of cell lines.

Domain

The CD6 binding site is located in the N-terminal Ig-like domain.

Sequence similarities

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 583556CD166 antigen
PRO_0000014660

Regions

Topological domain28 – 527500Extracellular Potential
Transmembrane528 – 54922Helical; Potential
Topological domain550 – 58334Cytoplasmic Potential
Domain28 – 12093Ig-like V-type 1
Domain125 – 234110Ig-like V-type 2
Domain245 – 32884Ig-like C2-type 1
Domain333 – 40977Ig-like C2-type 2
Domain416 – 50186Ig-like C2-type 3

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Ref.5
Glycosylation1671N-linked (GlcNAc...) Ref.5
Glycosylation2651N-linked (GlcNAc...) Potential
Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Glycosylation4571N-linked (GlcNAc...) Ref.5
Glycosylation4661N-linked (GlcNAc...); atypical Ref.5
Glycosylation4801N-linked (GlcNAc...) Potential
Glycosylation4961N-linked (GlcNAc...); atypical Ref.5
Glycosylation4991N-linked (GlcNAc...) Ref.5
Glycosylation5181N-linked (GlcNAc...); atypical Ref.5
Disulfide bond43 ↔ 113 Potential
Disulfide bond157 ↔ 220 Potential
Disulfide bond270 ↔ 313 Potential
Disulfide bond354 ↔ 392 Potential
Disulfide bond435 ↔ 485 Potential

Experimental info

Sequence conflict81S → C in BAC27159. Ref.2
Sequence conflict227 – 2326PSGQKT → AAGIPA in AAA37528. Ref.4
Sequence conflict3391S → R Ref.1
Sequence conflict3391S → R Ref.4
Sequence conflict4511G → S in BAC27159. Ref.2
Sequence conflict4541S → F in AAA37528. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q61490 [UniParc].

Last modified May 24, 2004. Version 3.
Checksum: 570AFA8FCAF888F8

FASTA58365,092
        10         20         30         40         50         60 
MASKVSPSCR LVFCLLISAA VLRPGLGWYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK 

        70         80         90        100        110        120 
PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI ANAKISDEKR FVCMLVTEDN 

       130        140        150        160        170        180 
VFEAPTLVKV FKQPSKPEIV NKAPFLETDQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV 

       190        200        210        220        230        240 
EGEVAILFKK EIDPGTQLYT VTSSLEYKTT RSDIQMPFTC SVTYYGPSGQ KTIYSEQEIF 

       250        260        270        280        290        300 
DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQPE GIRSSNTYTL 

       310        320        330        340        350        360 
TDVRRNATGD YKCSLIDKRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR 

       370        380        390        400        410        420 
NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM 

       430        440        450        460        470        480 
TKKTDPSGLS KTIICHVEGF PKPAIHWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN 

       490        500        510        520        530        540 
VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADDISDENRE KVNDQAKLIV GIVVGLLLAA 

       550        560        570        580 
LVAGVVYWLY MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of mouse ALCAM (CD166): the CD6 binding domain is conserved in different homologs and mediates cross-species binding."
Bowen M.A., Bajorath J., D'Egidio M., Whitney G.S., Palmer D., Kobarg J., Starling G.C., Siadak A.W., Aruffo A.
Eur. J. Immunol. 27:1469-1478(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: NFS.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The molecular cloning and characterization of potential chick DM-GRASP homologs in zebrafish and mouse."
Kanki J.P., Chang S., Kuwada J.Y.
J. Neurobiol. 25:831-845(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-583.
Strain: BALB/c.
Tissue: Brain.
[5]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-95; ASN-167; ASN-457; ASN-466; ASN-496; ASN-499 AND ASN-518.
Tissue: Myoblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U95030 mRNA. Translation: AAC06342.1.
AK030851 mRNA. Translation: BAC27159.1.
AK031391 mRNA. Translation: BAC27382.1.
BC027280 mRNA. Translation: AAH27280.1.
L25274 mRNA. Translation: AAA37528.1.
CCDSCCDS37356.1.
RefSeqNP_033785.1. NM_009655.2.
UniGeneMm.288282.

3D structure databases

ProteinModelPortalQ61490.
SMRQ61490. Positions 36-378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61490. 1 interaction.
MINTMINT-4997271.

Protein family/group databases

MEROPSI43.001.

PTM databases

PhosphoSiteQ61490.

Proteomic databases

MaxQBQ61490.
PaxDbQ61490.
PRIDEQ61490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023312; ENSMUSP00000023312; ENSMUSG00000022636.
GeneID11658.
KEGGmmu:11658.
UCSCuc007zli.2. mouse.

Organism-specific databases

CTD214.
MGIMGI:1313266. Alcam.

Phylogenomic databases

eggNOGNOG148902.
GeneTreeENSGT00530000063457.
HOGENOMHOG000070101.
HOVERGENHBG050847.
InParanoidQ61490.
KOK06547.
OMADIYYPTE.
OrthoDBEOG74J97B.
PhylomeDBQ61490.
TreeFamTF321859.

Gene expression databases

ArrayExpressQ61490.
BgeeQ61490.
CleanExMM_ALCAM.
GenevestigatorQ61490.

Family and domain databases

Gene3D2.60.40.10. 5 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTSM00409. IG. 3 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio279279.
PROQ61490.
SOURCESearch...

Entry information

Entry nameCD166_MOUSE
AccessionPrimary (citable) accession number: Q61490
Secondary accession number(s): O70136, Q8CDA5, Q8R2T0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 24, 2004
Last modified: July 9, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot