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Protein

Desert hedgehog protein

Gene

Dhh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi90 – 901Calcium 1By similarity
Metal bindingi91 – 911Calcium 1By similarity
Metal bindingi91 – 911Calcium 2By similarity
Metal bindingi96 – 961Calcium 1By similarity
Metal bindingi126 – 1261Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi127 – 1271Calcium 1By similarity
Metal bindingi127 – 1271Calcium 2By similarity
Metal bindingi130 – 1301Calcium 2By similarity
Metal bindingi132 – 1321Calcium 2By similarity
Metal bindingi141 – 1411ZincBy similarity
Metal bindingi148 – 1481ZincBy similarity
Metal bindingi183 – 1831ZincBy similarity
Sitei244 – 2441Involved in cholesterol transferBy similarity
Sitei268 – 2681Involved in auto-cleavageBy similarity
Sitei271 – 2711Essential for auto-cleavageBy similarity

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • patched binding Source: BHF-UCL
  • peptidase activity Source: UniProtKB-KW
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cell-cell signaling Source: InterPro
  • intein-mediated protein splicing Source: InterPro
  • Leydig cell differentiation Source: MGI
  • male sex determination Source: MGI
  • myelination Source: Ensembl
  • regulation of steroid biosynthetic process Source: MGI
  • response to estradiol Source: Ensembl
  • spermatid development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiC46.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Desert hedgehog protein
Short name:
DHH
Alternative name(s):
HHG-3
Cleaved into the following 2 chains:
Gene namesi
Name:Dhh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:94891. Dhh.

Subcellular locationi

Desert hedgehog protein N-product :
Desert hedgehog protein C-product :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 396374Desert hedgehog proteinPRO_0000013247Add
BLAST
Chaini23 – 198176Desert hedgehog protein N-productPRO_0000013248Add
BLAST
Chaini199 – 396198Desert hedgehog protein C-productPRO_0000013249Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi23 – 231N-palmitoyl cysteineBy similarity
Lipidationi198 – 1981Cholesterol glycine esterBy similarity

Post-translational modificationi

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei198 – 1992Cleavage; by autolysis

Keywords - PTMi

Autocatalytic cleavage, Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ61488.
PRIDEiQ61488.

PTM databases

iPTMnetiQ61488.

Expressioni

Tissue specificityi

Expressed in adult testes. Not expressed in limb buds.

Gene expression databases

BgeeiQ61488.
CleanExiMM_DHH.
ExpressionAtlasiQ61488. baseline and differential.
GenevisibleiQ61488. MM.

Interactioni

Subunit structurei

Interacts with BOC and CDON. Interacts with HHIP (By similarity).By similarity

GO - Molecular functioni

  • patched binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023737.

Structurei

3D structure databases

ProteinModelPortaliQ61488.
SMRiQ61488. Positions 39-189, 199-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The desert hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain.By similarity

Sequence similaritiesi

Belongs to the hedgehog family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3638. Eukaryota.
ENOG410XQA3. LUCA.
GeneTreeiENSGT00390000001117.
HOGENOMiHOG000233428.
HOVERGENiHBG005480.
InParanoidiQ61488.
KOiK11990.
OMAiPSMPERT.
OrthoDBiEOG779NZ5.
PhylomeDBiQ61488.
TreeFamiTF106458.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProiIPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_N.
[Graphical view]
PfamiPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFiPIRSF009400. Peptidase_C46. 1 hit.
PRINTSiPR00632. SONICHHOG.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.
PROSITEiPS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPASLLPL CCLALLALSA QSCGPGRGPV GRRRYVRKQL VPLLYKQFVP
60 70 80 90 100
SMPERTLGAS GPAEGRVTRG SERFRDLVPN YNPDIIFKDE ENSGADRLMT
110 120 130 140 150
ERCKERVNAL AIAVMNMWPG VRLRVTEGWD EDGHHAQDSL HYEGRALDIT
160 170 180 190 200
TSDRDRNKYG LLARLAVEAG FDWVYYESRN HIHVSVKADN SLAVRAGGCF
210 220 230 240 250
PGNATVRLRS GERKGLRELH RGDWVLAADA AGRVVPTPVL LFLDRDLQRR
260 270 280 290 300
ASFVAVETER PPRKLLLTPW HLVFAARGPA PAPGDFAPVF ARRLRAGDSV
310 320 330 340 350
LAPGGDALQP ARVARVAREE AVGVFAPLTA HGTLLVNDVL ASCYAVLESH
360 370 380 390
QWAHRAFAPL RLLHALGALL PGGAVQPTGM HWYSRLLYRL AEELMG
Length:396
Mass (Da):43,542
Last modified:November 1, 1996 - v1
Checksum:iAFFEB051BE950FD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76292 Genomic DNA. Translation: CAA53924.1.
CCDSiCCDS27810.1.
PIRiB49425.
RefSeqiNP_031883.1. NM_007857.5.
UniGeneiMm.324688.
Mm.384073.

Genome annotation databases

EnsembliENSMUST00000023737; ENSMUSP00000023737; ENSMUSG00000023000.
GeneIDi13363.
KEGGimmu:13363.
UCSCiuc007xoh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76292 Genomic DNA. Translation: CAA53924.1.
CCDSiCCDS27810.1.
PIRiB49425.
RefSeqiNP_031883.1. NM_007857.5.
UniGeneiMm.324688.
Mm.384073.

3D structure databases

ProteinModelPortaliQ61488.
SMRiQ61488. Positions 39-189, 199-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023737.

Protein family/group databases

MEROPSiC46.004.

PTM databases

iPTMnetiQ61488.

Proteomic databases

PaxDbiQ61488.
PRIDEiQ61488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023737; ENSMUSP00000023737; ENSMUSG00000023000.
GeneIDi13363.
KEGGimmu:13363.
UCSCiuc007xoh.2. mouse.

Organism-specific databases

CTDi50846.
MGIiMGI:94891. Dhh.

Phylogenomic databases

eggNOGiKOG3638. Eukaryota.
ENOG410XQA3. LUCA.
GeneTreeiENSGT00390000001117.
HOGENOMiHOG000233428.
HOVERGENiHBG005480.
InParanoidiQ61488.
KOiK11990.
OMAiPSMPERT.
OrthoDBiEOG779NZ5.
PhylomeDBiQ61488.
TreeFamiTF106458.

Miscellaneous databases

PROiQ61488.
SOURCEiSearch...

Gene expression databases

BgeeiQ61488.
CleanExiMM_DHH.
ExpressionAtlasiQ61488. baseline and differential.
GenevisibleiQ61488. MM.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProiIPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_N.
[Graphical view]
PfamiPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFiPIRSF009400. Peptidase_C46. 1 hit.
PRINTSiPR00632. SONICHHOG.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.
PROSITEiPS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity."
    Echelard Y., Epstein D.J., St Jacques B., Shen L., Mohler J., McMahon J.A., McMahon A.P.
    Cell 75:1417-1430(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 56-66, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  3. "Products, genetic linkage and limb patterning activity of a murine hedgehog gene."
    Chang D.T., Lopez A., von Kessler D.P., Chiang C., Simandl B.K., Zhao R., Seldin M.F., Fallon J.F., Beachy P.A.
    Development 120:3339-3353(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 120-168.

Entry informationi

Entry nameiDHH_MOUSE
AccessioniPrimary (citable) accession number: Q61488
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.