Reviewed, UniProtKB/Swiss-Prot Q61475 (DAF1_MOUSE)

Names and origin

Protein names

Recommended name:
    Complement decay-accelerating factor, GPI-anchored
      Short name=DAF-GPI
Alternative name(s):
    CD_antigen=CD55

Gene names

Name:	Cd55
Synonyms:	Cd55a, Daf, Daf1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protection of cells from complement-mediated damage By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Tissue specificity	Brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen and lymph node.
Domain	The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3 By similarity.
Sequence similarities	Belongs to the receptors of complement activation (RCA) family. Contains 4 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Biological process	Complement pathway Immune response Innate immunity
Cellular component	Cell membrane Membrane
Domain	Repeat Signal Sushi
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW external side of plasma membrane Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 362	328	Complement decay-accelerating factor, GPI-anchored		PRO_0000006004
	Propeptide	363 – 390	28	Removed in mature form Potential		PRO_0000006005
Regions
	Domain	35 – 96	62	Sushi 1
	Domain	97 – 160	64	Sushi 2
	Domain	161 – 222	62	Sushi 3
	Domain	223 – 286	64	Sushi 4
	Compositional bias	288 – 362	75	Ser/Thr-rich
Amino acid modifications
	Lipidation	362	1	GPI-anchor amidated glycine Potential
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
	Disulfide bond	36 ↔ 81		By similarity
	Disulfide bond	65 ↔ 94		By similarity
	Disulfide bond	98 ↔ 145		By similarity
	Disulfide bond	129 ↔ 158		By similarity
	Disulfide bond	163 ↔ 204		By similarity
	Disulfide bond	190 ↔ 220		By similarity
	Disulfide bond	225 ↔ 267		By similarity
	Disulfide bond	253 ↔ 284		By similarity
Experimental info
	Sequence conflict	7 – 9	3	PRT → ARA in BAA09830. Ref.5
	Sequence conflict	83	1	E → G in BAA09830. Ref.5
	Sequence conflict	91	1	E → G in BAA09830. Ref.5
	Sequence conflict	98	1	C → L in AAH11314. Ref.4
	Sequence conflict	115	1	N → H in AAH11314. Ref.4
	Sequence conflict	135	1	K → E in AAB00091. Ref.1
	Sequence conflict	143	1	A → S in CAJ18536. Ref.3
	Sequence conflict	143	1	A → S in AAH11314. Ref.4
	Sequence conflict	173	1	H → L in BAA09830. Ref.5
	Sequence conflict	180	1	I → T in BAA09830. Ref.5
	Sequence conflict	258	1	I → V in CAJ18536. Ref.3
	Sequence conflict	258	1	I → V in AAH11314. Ref.4
	Sequence conflict	313	1	T → L in AAH11314. Ref.4
	Sequence conflict	381	1	V → A in CAJ18536. Ref.3
	Sequence conflict	381	1	V → A in AAH11314. Ref.4
	Sequence conflict	381	1	V → A in AAD51449. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q61475-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: B4B872186947F8E4	FASTA	390	42,618
	10 20 30 40 50 60 MIRGRAPRTR PSPPPPLLPL LSLSLLLLSP TVRGDCGPPP DIPNARPILG RHSKFAEQSK 70 80 90 100 110 120 VAYSCNNGFK QVPDKSNIVV CLENGQWSSH ETFCEKSCVA PERLSFASLK KEYLNMNFFP 130 140 150 160 170 180 VGTIVEYECR PGFRKQPPLP GKATCLEDLV WSPVAQFCKK KSCPNPKDLD NGHINIPTGI 190 200 210 220 230 240 LFGSEINFSC NPGYRLVGVS STFCSVTGNT VDWDDEFPVC TEIHCPEPPK INNGIMRGES 250 260 270 280 290 300 DSYTYSQVVT YSCDKGFILV GNASIYCTVS KSDVGQWSSP PPRCIEKSKV PTKKPTINVP 310 320 330 340 350 360 STGTPSTPQK PTTESVPNPG DQPTPQKPST VKVSATQHVP VTKTTVRHPI RTSTDKGEPN 370 380 390 TGGDRYIYGH TCLITLTVLH VMLSLIGYLT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and chromosomal localization of the mouse decay-accelerating factor genes. Duplicated genes encode glycosylphosphatidylinositol-anchored and transmembrane forms." Spicer A.P., Seldin M.F., Gendler S.J. J. Immunol. 155:3079-3091(1995) [PubMed: 7545711] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Testis.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
[3]	"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)." Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
[5]	"Molecular cloning of murine decay accelerating factor by immunoscreening." Fukuoka Y., Yasui A., Okada N., Okada H. Int. Immunol. 8:379-385(1996) [PubMed: 8671624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-390. Strain: BALB/c. Tissue: Spleen.
[6]	"A new repetitive sequence uniquely present in the decay-accelerating factor genes." Nonaka M., Nonaka M., Takenaka O., Okada N., Okada H. Immunogenetics 47:246-255(1998) [PubMed: 9435343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 315-353. Strain: BALB/c.
[7]	"Molecular and functional analysis of mouse decay accelerating factor (CD55)." Harris C.L., Rushmere N.K., Morgan B.P. Biochem. J. 341:821-829(1999) [PubMed: 10417349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 369-390. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L41366 mRNA. Translation: AAB00091.1. AK160994 mRNA. Translation: BAE36139.1. CT010328 mRNA. Translation: CAJ18536.1. BC011314 mRNA. Translation: AAH11314.1. D63679 mRNA. Translation: BAA09830.1. AB003320 Genomic DNA. Translation: BAA22908.1. AF143541 mRNA. Translation: AAD51449.1.
IPI	IPI00322234.
RefSeq	NP_034146.2.
UniGene	Mm.101591
3D structure databases
HSSP	HSSP built from PDB template 1H03 based on UniProtKB P08174.
ModBase	Search...
Proteomic databases
PRIDE	Q61475.
Genome annotation databases
Ensembl	ENSMUSG00000026399. Mus musculus. [Contig view]
GeneID	13136.
KEGG	mmu:13136.
Organism-specific databases
MGI	MGI:104850. Cd55.
Phylogenomic databases
HOGENOM	Q61475.
HOVERGEN	Q61475.
OMA	Q61475. NDDEGEW.
Gene expression databases
ArrayExpress	Q61475.
Bgee	Q61475.
CleanEx	MM_CD55.
GermOnline	ENSMUSG00000026399. Mus musculus.
Family and domain databases
InterPro	IPR016060. Complement_control_module. IPR000436. Sushi_SCR_CCP. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 4 hits.
Pfam	PF00084. Sushi. 4 hits. [Graphical view]
SMART	SM00032. CCP. 4 hits. [Graphical view]
PROSITE	PS50923. SUSHI. 4 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283202.
SOURCE	Search...

Entry information

Entry name

DAF1_MOUSE

Accession

Primary (citable) accession number: Q61475
Secondary accession number(s): P97732 , Q3TU32, Q4FJS4, Q61397, Q76N72, Q921P0, Q9R1C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)