Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA-binding protein Musashi homolog 1

Gene

Msi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.2 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI
  • poly(U) RNA binding Source: MGI
  • single-stranded RNA binding Source: MGI
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein Musashi homolog 1
Short name:
Musashi-1
Gene namesi
Name:Msi1
Synonyms:Msi1h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:107376. Msi1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • polysome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63F → L: Abolishes RNA binding; when associated with L-65 and L-68. 1 Publication1
Mutagenesisi65F → L: Abolishes RNA binding; when associated with L-63 and L-68. 1 Publication1
Mutagenesisi68F → L: Abolishes RNA binding; when associated with L-63 and L-65. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816501 – 362RNA-binding protein Musashi homolog 1Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei191PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ61474.
PeptideAtlasiQ61474.
PRIDEiQ61474.

PTM databases

iPTMnetiQ61474.
PhosphoSitePlusiQ61474.

Expressioni

Tissue specificityi

Detected in olfactory bulb, brain stem, small intestine, and at low levels in brain cortex, hippocampus and ovary. Detected in neural progenitor cells, including neural stem cells.1 Publication

Developmental stagei

Highly expressed in embryonic brain at day 12. Expressed at intermediate levels during the rest of embryonic development and in newborns up to day 3. After this expression decreases and stabilizes at low levels of expression around day 13.

Gene expression databases

BgeeiENSMUSG00000054256.
CleanExiMM_MSI1.
ExpressionAtlasiQ61474. baseline and differential.
GenevisibleiQ61474. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8327453,EBI-8327453

Protein-protein interaction databases

IntActiQ61474. 5 interactors.
MINTiMINT-4102437.
STRINGi10090.ENSMUSP00000120516.

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 26Combined sources5
Helixi34 – 40Combined sources7
Turni41 – 43Combined sources3
Beta strandi49 – 52Combined sources4
Beta strandi57 – 59Combined sources3
Beta strandi61 – 66Combined sources6
Helixi73 – 79Combined sources7
Turni80 – 82Combined sources3
Beta strandi83 – 85Combined sources3
Beta strandi91 – 94Combined sources4
Beta strandi111 – 114Combined sources4
Helixi122 – 130Combined sources9
Beta strandi136 – 138Combined sources3
Beta strandi144 – 146Combined sources3
Beta strandi151 – 156Combined sources6
Helixi160 – 167Combined sources8
Beta strandi168 – 170Combined sources3
Beta strandi174 – 176Combined sources3
Beta strandi180 – 182Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UAWNMR-A20-96[»]
2MSSNMR-A110-184[»]
2MSTNMR-A110-184[»]
2RS2NMR-A20-103[»]
ProteinModelPortaliQ61474.
SMRiQ61474.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61474.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 110RRM 1PROSITE-ProRule annotationAdd BLAST91
Domaini109 – 186RRM 2PROSITE-ProRule annotationAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi274 – 281Poly-Ala8

Domaini

The first RNA recognition motif binds more strongly to RNA compared to the second one.

Sequence similaritiesi

Belongs to the Musashi family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4205. Eukaryota.
ENOG410YA8Z. LUCA.
GeneTreeiENSGT00760000118873.
HOVERGENiHBG002295.
InParanoidiQ61474.
KOiK14411.
OMAiHYFEQFG.
OrthoDBiEOG091G1CPI.
PhylomeDBiQ61474.
TreeFamiTF325419.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61474-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL
60 70 80 90 100
VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA
110 120 130 140 150
QPKMVTRTKK IFVGGLSVNT TVEDVKHYFE QFGKVDDAML MFDKTTNRHR
160 170 180 190 200
GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS
210 220 230 240 250
RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER
260 270 280 290 300
SPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST
310 320 330 340 350
PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG
360
PLIATAFTNG YH
Length:362
Mass (Da):39,119
Last modified:November 1, 1996 - v1
Checksum:i75C316BB384AE211
GO
Isoform 2 (identifier: Q61474-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.
     264-362: AIPLTAYGPM...IATAFTNGYH → GQWLRFRASH...GREAKPGRRR

Note: No experimental confirmation available.
Show »
Length:301
Mass (Da):34,029
Checksum:i9DE83A292A51E4B1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0111661 – 21Missing in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_011167264 – 362AIPLT…TNGYH → GQWLRFRASHRPRGEKGGER DCPLPRGEAGRTTVPGHWPV SSATWPSRVGREAKPGRRR in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49654 mRNA. Translation: BAA08530.1.
AK084019 mRNA. Translation: BAC39099.1.
CCDSiCCDS19591.1. [Q61474-1]
RefSeqiNP_032655.1. NM_008629.1. [Q61474-1]
UniGeneiMm.391995.
Mm.5077.

Genome annotation databases

EnsembliENSMUST00000150779; ENSMUSP00000120516; ENSMUSG00000054256. [Q61474-1]
GeneIDi17690.
KEGGimmu:17690.
UCSCiuc008zdu.1. mouse. [Q61474-2]
uc008zdv.1. mouse. [Q61474-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49654 mRNA. Translation: BAA08530.1.
AK084019 mRNA. Translation: BAC39099.1.
CCDSiCCDS19591.1. [Q61474-1]
RefSeqiNP_032655.1. NM_008629.1. [Q61474-1]
UniGeneiMm.391995.
Mm.5077.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UAWNMR-A20-96[»]
2MSSNMR-A110-184[»]
2MSTNMR-A110-184[»]
2RS2NMR-A20-103[»]
ProteinModelPortaliQ61474.
SMRiQ61474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61474. 5 interactors.
MINTiMINT-4102437.
STRINGi10090.ENSMUSP00000120516.

PTM databases

iPTMnetiQ61474.
PhosphoSitePlusiQ61474.

Proteomic databases

PaxDbiQ61474.
PeptideAtlasiQ61474.
PRIDEiQ61474.

Protocols and materials databases

DNASUi17690.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000150779; ENSMUSP00000120516; ENSMUSG00000054256. [Q61474-1]
GeneIDi17690.
KEGGimmu:17690.
UCSCiuc008zdu.1. mouse. [Q61474-2]
uc008zdv.1. mouse. [Q61474-1]

Organism-specific databases

CTDi4440.
MGIiMGI:107376. Msi1.

Phylogenomic databases

eggNOGiKOG4205. Eukaryota.
ENOG410YA8Z. LUCA.
GeneTreeiENSGT00760000118873.
HOVERGENiHBG002295.
InParanoidiQ61474.
KOiK14411.
OMAiHYFEQFG.
OrthoDBiEOG091G1CPI.
PhylomeDBiQ61474.
TreeFamiTF325419.

Miscellaneous databases

EvolutionaryTraceiQ61474.
PROiQ61474.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054256.
CleanExiMM_MSI1.
ExpressionAtlasiQ61474. baseline and differential.
GenevisibleiQ61474. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSI1H_MOUSE
AccessioniPrimary (citable) accession number: Q61474
Secondary accession number(s): Q8BNC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.