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Protein

Transcription factor SOX-17

Gene

Sox17

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells. Isoform 2 (T-SOX17) shows no DNA-binding activity.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi68 – 13669HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • DNA binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: MGI
  • transcription coactivator activity Source: MGI
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription factor binding Source: MGI
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

  • angiogenesis Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • cardiac cell fate determination Source: MGI
  • cardiogenic plate morphogenesis Source: BHF-UCL
  • cell migration involved in gastrulation Source: MGI
  • common bile duct development Source: MGI
  • embryonic foregut morphogenesis Source: BHF-UCL
  • embryonic heart tube development Source: MGI
  • embryonic heart tube morphogenesis Source: BHF-UCL
  • embryonic organ development Source: MGI
  • endocardial cell differentiation Source: MGI
  • endocardium formation Source: MGI
  • endodermal cell fate determination Source: MGI
  • endodermal digestive tract morphogenesis Source: BHF-UCL
  • endoderm development Source: UniProtKB
  • endoderm formation Source: MGI
  • gall bladder development Source: MGI
  • gastrulation Source: MGI
  • heart looping Source: UniProtKB
  • inner cell mass cellular morphogenesis Source: MGI
  • mRNA transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of canonical Wnt signaling pathway Source: MGI
  • negative regulation of cell growth Source: MGI
  • negative regulation of mesodermal cell fate specification Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of Wnt signaling pathway Source: UniProtKB
  • negative regulation of Wnt signaling pathway involved in heart development Source: BHF-UCL
  • outflow tract morphogenesis Source: BHF-UCL
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of protein catabolic process Source: UniProtKB
  • positive regulation of skeletal muscle tissue development Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein destabilization Source: MGI
  • protein stabilization Source: MGI
  • regulation of cardiac cell fate specification Source: BHF-UCL
  • regulation of cell differentiation Source: MGI
  • regulation of embryonic development Source: UniProtKB
  • regulation of mesodermal cell fate specification Source: MGI
  • regulation of stem cell division Source: MGI
  • regulation of stem cell proliferation Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter involved in definitive endodermal cell fate specification Source: BHF-UCL
  • renal system development Source: MGI
  • rostrocaudal neural tube patterning Source: MGI
  • signal transduction involved in regulation of gene expression Source: MGI
  • spermatogenesis Source: MGI
  • stem cell differentiation Source: MGI
  • stem cell fate specification Source: MGI
  • vasculogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-3769402. Deactivation of the beta-catenin transactivating complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SOX-17
Gene namesi
Name:Sox17
Synonyms:Sox-17
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107543. Sox17.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nuclear transcription factor complex Source: MGI
  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal due to defects in the development of the definite gut endoderm and aberrant heart looping. Embryos die at about 10.5 dpc.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Transcription factor SOX-17PRO_0000048766Add
BLAST

Proteomic databases

PaxDbiQ61473.
PRIDEiQ61473.

PTM databases

iPTMnetiQ61473.
PhosphoSiteiQ61473.

Expressioni

Tissue specificityi

Testis. Detected in lung.

Developmental stagei

Detected in the extraembryonic region of the visceral endoderm of pre-streak and early-streak embryos. Detected in the extraembryonic region of the visceral endoderm and in the definitive endoderm at 7.5 dpc. By the seven to eight somite stage, detected in the posterior endoderm, mainly in the endoderm of the midgut and hindgut invagination. Expressed in spermatogonia. The expression clearly declines from the early pachytene spermatocyte stage onward. In contrast, expression of isoform 2 (T-SOX17) begins at the pachytene spermatocyte stage and is highly accumulated in round spermatids.1 Publication

Gene expression databases

BgeeiENSMUSG00000025902.
CleanExiMM_SOX17.
ExpressionAtlasiQ61473. baseline and differential.
GenevisibleiQ61473. MM.

Interactioni

Subunit structurei

Interacts with CTNNB1, LEF1 and TCF4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ctnnb1P262333EBI-9106822,EBI-7373758From a different organism.
tcf3Q019783EBI-9106822,EBI-9106902From a different organism.
TCF4P158845EBI-9106822,EBI-533224From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203403. 1 interaction.
IntActiQ61473. 7 interactions.
STRINGi10090.ENSMUSP00000027035.

Structurei

Secondary structure

1
419
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi74 – 8916Combined sources
Beta strandi91 – 933Combined sources
Helixi95 – 10814Combined sources
Helixi111 – 13121Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F27X-ray2.75D66-144[»]
ProteinModelPortaliQ61473.
SMRiQ61473. Positions 68-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini278 – 418141Sox C-terminalPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi312 – 34635Gln/Pro-richAdd
BLAST
Compositional biasi322 – 3265Poly-Pro

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation
Contains 1 Sox C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IPZH. Eukaryota.
ENOG41114B8. LUCA.
GeneTreeiENSGT00760000118988.
HOGENOMiHOG000069999.
HOVERGENiHBG000517.
InParanoidiQ61473.
KOiK04495.
OrthoDBiEOG091G0CYU.
PhylomeDBiQ61473.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
IPR033392. Sox7/17/18_central.
IPR021934. Sox_C.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF12067. Sox17_18_mid. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
PS51516. SOX_C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61473-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSPDAGYAS DDQSQPRSAQ PAVMAGLGPC PWAESLSPLG DVKVKGEVVA
60 70 80 90 100
SSGAPAGTSG RAKAESRIRR PMNAFMVWAK DERKRLAQQN PDLHNAELSK
110 120 130 140 150
MLGKSWKALT LAEKRPFVEE AERLRVQHMQ DHPNYKYRPR RRKQVKRMKR
160 170 180 190 200
VEGGFLHALV EPQAGALGPE GGRVAMDGLG LPFPEPGYPA GPPLMSPHMG
210 220 230 240 250
PHYRDCQGLG APALDGYPLP TPDTSPLDGV EQDPAFFAAP LPGDCPAAGT
260 270 280 290 300
YTYAPVSDYA VSVEPPAGPM RVGPDPSGPA MPGILAPPSA LHLYYGAMGS
310 320 330 340 350
PAASAGRGFH AQPQQPLQPQ APPPPPQQQH PAHGPGQPSP PPEALPCRDG
360 370 380 390 400
TESNQPTELL GEVDRTEFEQ YLPFVYKPEM GLPYQGHDCG VNLSDSHGAI
410
SSVVSDASSA VYYCNYPDI
Length:419
Mass (Da):44,646
Last modified:November 1, 1997 - v1
Checksum:i14233EED2CCDD69D
GO
Isoform 2 (identifier: Q61473-2) [UniParc]FASTAAdd to basket
Also known as: T-SOX17

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.

Show »
Length:291
Mass (Da):30,782
Checksum:i2298C8A3EC02F44C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 128128Missing in isoform 2. 1 PublicationVSP_002204Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49474 mRNA. Translation: BAA08444.1.
D49473 mRNA. Translation: BAA08443.1.
AK004781 mRNA. Translation: BAB23559.1.
L29085 mRNA. Translation: AAA56843.1.
CCDSiCCDS14805.1. [Q61473-1]
CCDS78538.1. [Q61473-2]
RefSeqiNP_001276393.1. NM_001289464.1. [Q61473-1]
NP_001276394.1. NM_001289465.1.
NP_001276396.1. NM_001289467.1. [Q61473-2]
NP_035571.1. NM_011441.5. [Q61473-1]
XP_006495537.1. XM_006495474.2. [Q61473-1]
XP_006495538.1. XM_006495475.2. [Q61473-1]
XP_011236665.1. XM_011238363.1. [Q61473-1]
XP_011236666.1. XM_011238364.1. [Q61473-1]
UniGeneiMm.279103.

Genome annotation databases

EnsembliENSMUST00000027035; ENSMUSP00000027035; ENSMUSG00000025902. [Q61473-1]
ENSMUST00000116652; ENSMUSP00000112351; ENSMUSG00000025902. [Q61473-1]
ENSMUST00000195555; ENSMUSP00000141894; ENSMUSG00000025902. [Q61473-2]
GeneIDi20671.
KEGGimmu:20671.
UCSCiuc007aey.1. mouse. [Q61473-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49474 mRNA. Translation: BAA08444.1.
D49473 mRNA. Translation: BAA08443.1.
AK004781 mRNA. Translation: BAB23559.1.
L29085 mRNA. Translation: AAA56843.1.
CCDSiCCDS14805.1. [Q61473-1]
CCDS78538.1. [Q61473-2]
RefSeqiNP_001276393.1. NM_001289464.1. [Q61473-1]
NP_001276394.1. NM_001289465.1.
NP_001276396.1. NM_001289467.1. [Q61473-2]
NP_035571.1. NM_011441.5. [Q61473-1]
XP_006495537.1. XM_006495474.2. [Q61473-1]
XP_006495538.1. XM_006495475.2. [Q61473-1]
XP_011236665.1. XM_011238363.1. [Q61473-1]
XP_011236666.1. XM_011238364.1. [Q61473-1]
UniGeneiMm.279103.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F27X-ray2.75D66-144[»]
ProteinModelPortaliQ61473.
SMRiQ61473. Positions 68-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203403. 1 interaction.
IntActiQ61473. 7 interactions.
STRINGi10090.ENSMUSP00000027035.

PTM databases

iPTMnetiQ61473.
PhosphoSiteiQ61473.

Proteomic databases

PaxDbiQ61473.
PRIDEiQ61473.

Protocols and materials databases

DNASUi20671.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027035; ENSMUSP00000027035; ENSMUSG00000025902. [Q61473-1]
ENSMUST00000116652; ENSMUSP00000112351; ENSMUSG00000025902. [Q61473-1]
ENSMUST00000195555; ENSMUSP00000141894; ENSMUSG00000025902. [Q61473-2]
GeneIDi20671.
KEGGimmu:20671.
UCSCiuc007aey.1. mouse. [Q61473-1]

Organism-specific databases

CTDi64321.
MGIiMGI:107543. Sox17.

Phylogenomic databases

eggNOGiENOG410IPZH. Eukaryota.
ENOG41114B8. LUCA.
GeneTreeiENSGT00760000118988.
HOGENOMiHOG000069999.
HOVERGENiHBG000517.
InParanoidiQ61473.
KOiK04495.
OrthoDBiEOG091G0CYU.
PhylomeDBiQ61473.

Enzyme and pathway databases

ReactomeiR-MMU-3769402. Deactivation of the beta-catenin transactivating complex.

Miscellaneous databases

PROiQ61473.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025902.
CleanExiMM_SOX17.
ExpressionAtlasiQ61473. baseline and differential.
GenevisibleiQ61473. MM.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
IPR033392. Sox7/17/18_central.
IPR021934. Sox_C.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF12067. Sox17_18_mid. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
PS51516. SOX_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSOX17_MOUSE
AccessioniPrimary (citable) accession number: Q61473
Secondary accession number(s): Q61472, Q62248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.