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Protein

Protein Tob1

Gene

Tob1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex.By similarity

GO - Molecular functioni

  • receptor tyrosine kinase binding Source: UniProtKB
  • SMAD binding Source: MGI
  • transcription corepressor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Tob1
Alternative name(s):
Transducer of erbB-2 1
Gene namesi
Name:Tob1
Synonyms:Tob, Trob
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1349721. Tob1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Only a small fraction localizes to the cytoplasm except in late S-phase where more than half of proteins become cytoplasmic.

GO - Cellular componenti

  • CCR4-NOT complex Source: UniProtKB
  • cytoplasm Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi22 – 243RRR → QQQ: Localizes to cytoplasm; when associated with 37-NNN-39. 1 Publication
Mutagenesisi37 – 393KKK → NNN: Localizes to cytoplasm; when associated with 22-QQQ-24. 1 Publication
Mutagenesisi234 – 2341L → A: Not exported to the cytoplasm; when associated with A-236. 1 Publication
Mutagenesisi236 – 2361L → A: Not exported to the cytoplasm; when associated with A-234. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Protein Tob1PRO_0000143813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei204 – 2041PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on Ser and Thr residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ61471.
PRIDEiQ61471.

PTM databases

PhosphoSiteiQ61471.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

CleanExiMM_TOB1.
GenevisibleiQ61471. MM.

Interactioni

Subunit structurei

Interacts with ERBB2. Interacts with CNOT7 and CNOT8 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CORO7P577373EBI-8527498,EBI-6916167From a different organism.

GO - Molecular functioni

  • receptor tyrosine kinase binding Source: UniProtKB
  • SMAD binding Source: MGI

Protein-protein interaction databases

BioGridi204322. 2 interactions.
IntActiQ61471. 1 interaction.
STRINGi10090.ENSMUSP00000036039.

Structurei

3D structure databases

ProteinModelPortaliQ61471.
SMRiQ61471. Positions 1-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 9211Important for nuclear localizationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi22 – 3918Bipartite nuclear localization signalAdd
BLAST
Motifi228 – 2369Nuclear export signal

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi243 – 25311Poly-GlnAdd
BLAST
Compositional biasi259 – 2646Poly-Pro
Compositional biasi265 – 28016Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the BTG family.Curated

Phylogenomic databases

eggNOGiKOG4006. Eukaryota.
ENOG410ZZC0. LUCA.
GeneTreeiENSGT00550000074461.
HOGENOMiHOG000253939.
HOVERGENiHBG006617.
InParanoidiQ61471.
KOiK14443.
OMAiSIFPGDS.
OrthoDBiEOG7VTDNJ.
TreeFamiTF105274.

Family and domain databases

InterProiIPR002087. Anti_prolifrtn.
IPR015677. Tob1.
IPR015676. Tob1/2.
[Graphical view]
PANTHERiPTHR17537. PTHR17537. 2 hits.
PTHR17537:SF6. PTHR17537:SF6. 2 hits.
PfamiPF07742. BTG. 1 hit.
[Graphical view]
PRINTSiPR00310. ANTIPRLFBTG1.
SMARTiSM00099. btg1. 1 hit.
[Graphical view]
SUPFAMiSSF160696. SSF160696. 1 hit.
PROSITEiPS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLEIQVALN FIISYLYNKL PRRRVNIFGE ELERLLKKKY EGHWYPEKPY
60 70 80 90 100
KGSGFRCIHV GEKVDPVIEQ ASKESGLDID DVRGNLPQDL SVWIDPFEVS
110 120 130 140 150
YQIGEKGPVK VLYVDDSSET GCELDKEIKN SFNPEAQVFM PISDPASSVS
160 170 180 190 200
SSPSPPFGHS AAVSPTFMPR STQPLTFTTA TFAATKFGST KMKNSGRSSK
210 220 230 240 250
VARTSPINLG LTVNVNDLLK QKAISSSVHS LYGLGLGSQQ QPQPQPQQQQ
260 270 280 290 300
QQQPSSSQPP PPLPQQQQQQ PQQQQQQQQQ TSALSPNAKE FIFPNMQGQG
310 320 330 340 350
SSTNGMFPGD SPLNLSPLQY SNAFDVFAAY GGLNEKSFVD GLNFSLNNMQ
360
YSNQQFQPVM AN
Length:362
Mass (Da):40,056
Last modified:September 18, 2013 - v2
Checksum:i4D4F1F492A7CA6FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361A → P in BAA11384 (PubMed:9210596).Curated
Sequence conflicti217 – 2171D → H in BAA11384 (PubMed:9210596).Curated
Sequence conflicti271 – 2711P → PQ in BAA11384 (PubMed:9210596).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78382 Genomic DNA. Translation: BAA11384.1.
AL645846 Genomic DNA. No translation available.
BC082588 mRNA. Translation: AAH82588.1.
CCDSiCCDS25250.1.
RefSeqiNP_033453.2. NM_009427.2.
UniGeneiMm.259673.

Genome annotation databases

EnsembliENSMUST00000041589; ENSMUSP00000036039; ENSMUSG00000037573.
GeneIDi22057.
KEGGimmu:22057.
UCSCiuc007kyf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78382 Genomic DNA. Translation: BAA11384.1.
AL645846 Genomic DNA. No translation available.
BC082588 mRNA. Translation: AAH82588.1.
CCDSiCCDS25250.1.
RefSeqiNP_033453.2. NM_009427.2.
UniGeneiMm.259673.

3D structure databases

ProteinModelPortaliQ61471.
SMRiQ61471. Positions 1-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204322. 2 interactions.
IntActiQ61471. 1 interaction.
STRINGi10090.ENSMUSP00000036039.

PTM databases

PhosphoSiteiQ61471.

Proteomic databases

PaxDbiQ61471.
PRIDEiQ61471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041589; ENSMUSP00000036039; ENSMUSG00000037573.
GeneIDi22057.
KEGGimmu:22057.
UCSCiuc007kyf.1. mouse.

Organism-specific databases

CTDi10140.
MGIiMGI:1349721. Tob1.

Phylogenomic databases

eggNOGiKOG4006. Eukaryota.
ENOG410ZZC0. LUCA.
GeneTreeiENSGT00550000074461.
HOGENOMiHOG000253939.
HOVERGENiHBG006617.
InParanoidiQ61471.
KOiK14443.
OMAiSIFPGDS.
OrthoDBiEOG7VTDNJ.
TreeFamiTF105274.

Miscellaneous databases

PROiQ61471.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TOB1.
GenevisibleiQ61471. MM.

Family and domain databases

InterProiIPR002087. Anti_prolifrtn.
IPR015677. Tob1.
IPR015676. Tob1/2.
[Graphical view]
PANTHERiPTHR17537. PTHR17537. 2 hits.
PTHR17537:SF6. PTHR17537:SF6. 2 hits.
PfamiPF07742. BTG. 1 hit.
[Graphical view]
PRINTSiPR00310. ANTIPRLFBTG1.
SMARTiSM00099. btg1. 1 hit.
[Graphical view]
SUPFAMiSSF160696. SSF160696. 1 hit.
PROSITEiPS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the mouse tob gene."
    Yoshida Y., Matuda S., Yamamoto T.
    Gene 191:109-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Nuclear localization of Tob is important for regulation of its antiproliferative activity."
    Kawamura-Tsuzuku J., Suzuki T., Yoshida Y., Yamamoto T.
    Oncogene 23:6630-6638(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, NUCLEAR EXPORT SIGNAL, MUTAGENESIS OF 22-ARG--ARG-24; 37-LYS--LYS-39; LEU-234 AND LEU-236.

Entry informationi

Entry nameiTOB1_MOUSE
AccessioniPrimary (citable) accession number: Q61471
Secondary accession number(s): Q640M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 18, 2013
Last modified: June 8, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.