Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q61468 (MSLN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mesothelin
Alternative name(s):
Pre-pro-megakaryocyte-potentiating factor

Cleaved into the following 2 chains:

  1. Megakaryocyte-potentiating factor
    Short name=MPF
  2. Mesothelin, cleaved form
Gene names
Name:Msln
Synonyms:Mes, Mpf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Membrane-anchored forms may play a role in cellular adhesion. Ref.4 Ref.6

Megakaryocyte-potentiating factor (MPF) may potentiate megakaryocyte colony formation By similarity. Ref.4 Ref.6

Subunit structure

Interacts with MUC16. Ref.6

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity. Golgi apparatus By similarity.

Megakaryocyte-potentiating factor: Secreted By similarity.

Tissue specificity

Highly expressed in lung and heart. Expressed at low levels in spleen, liver, kidney and testis. Present in lung (at protein level). Ref.4

Developmental stage

Expressed at E7, down-regulated at E11, re-expressed at E15 and peaks at E17. Present in embryonic diaphragm (at protein level). Ref.4 Ref.6

Induction

By WNT1 but not by WNT5A in mammary epithelial cells. Ref.5

Post-translational modification

Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin By similarity.

Disruption phenotype

Mice have normal growth and reproductive function, and normal platelet counts. Ref.4

Sequence similarities

Belongs to the mesothelin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Chain36 – 600565Mesothelin
PRO_0000253562
Chain36 – 288253Megakaryocyte-potentiating factor
PRO_0000253563
Chain298 – 600303Mesothelin, cleaved form
PRO_0000253564
Propeptide601 – 62525Removed in mature form Potential
PRO_0000253565

Amino acid modifications

Lipidation6001GPI-anchor amidated serine Potential
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation3901N-linked (GlcNAc...) Potential
Glycosylation4881N-linked (GlcNAc...) Potential
Glycosylation5171N-linked (GlcNAc...) Potential
Disulfide bond304 ↔ 328 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q61468 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9A5E9C3222C6983F

FASTA62569,423
        10         20         30         40         50         60 
MALPTARPLL GSCGSPICSR SFLLLLLSLG WIPRLQTQTT KTSQEATLLH AVNGAADFAS 

        70         80         90        100        110        120 
LPTGLFLGLT CEEVSDLSME QAKGLAMAVR QKNITLRGHQ LRCLARRLPR HLTDEELNAL 

       130        140        150        160        170        180 
PLDLLLFLNP AMFPGQQACA HFFSLISKAN VDVLPRRSLE RQRLLMEALK CQGVYGFQVS 

       190        200        210        220        230        240 
EADVRALGGL ACDLPGKFVA RSSEVLLPWL AGCQGPLDQS QEKAVREVLR SGRTQYGPPS 

       250        260        270        280        290        300 
KWSVSTLDAL QSLVAVLDES IVQSIPKDVK AEWLQHISRD PSRLGSKLTV IHPRFRRDAE 

       310        320        330        340        350        360 
QKACPPGKEP YKVDEDLIFY QNWELEACVD GTMLARQMDL VNEIPFTYEQ LSIFKHKLDK 

       370        380        390        400        410        420 
TYPQGYPESL IQQLGHFFRY VSPEDIHQWN VTSPDTVKTL LKVSKGQKMN AQAIALVACY 

       430        440        450        460        470        480 
LRGGGQLDED MVKALGDIPL SYLCDFSPQD LHSVPSSVMW LVGPQDLDKC SQRHLGLLYQ 

       490        500        510        520        530        540 
KACSAFQNVS GLEYFEKIKT FLGGASVKDL RALSQHNVSM DIATFKRLQV DSLVGLSVAE 

       550        560        570        580        590        600 
VQKLLGPNIV DLKTEEDKSP VRDWLFRQHQ KDLDRLGLGL QGGIPNGYLV LDFNVREAFS 

       610        620 
SRASLLGPGF VLIWIPALLP ALRLS 

« Hide

References

« Hide 'large scale' references
[1]"Mouse megakaryocyte potentiating factor cDNA."
Kojima T., Taniguchi Y., Hattori K., Oh-eda M.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Seminal vesicle.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Mesothelin is not required for normal mouse development or reproduction."
Bera T.K., Pastan I.
Mol. Cell. Biol. 20:2902-2906(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[5]"Stromelysin-1 and mesothelin are differentially regulated by Wnt-5a and Wnt-1 in C57mg mouse mammary epithelial cells."
Prieve M.G., Moon R.T.
BMC Dev. Biol. 3:2-2(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY WNT1.
[6]"Binding of ovarian cancer antigen CA125/MUC16 to mesothelin mediates cell adhesion."
Rump A., Morikawa Y., Tanaka M., Minami S., Umesaki N., Takeuchi M., Miyajima A.
J. Biol. Chem. 279:9190-9198(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MUC16, DEVELOPMENTAL STAGE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86370 mRNA. Translation: BAA13077.1.
AK146799 mRNA. Translation: BAE27441.1.
AK144391 mRNA. Translation: BAE25864.1.
BC023753 mRNA. Translation: AAH23753.1.
CCDSCCDS28526.1.
RefSeqNP_061345.1. NM_018857.1.
XP_006524713.1. XM_006524650.1.
UniGeneMm.17510.

3D structure databases

ProteinModelPortalQ61468.
SMRQ61468. Positions 304-360.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61468. 1 interaction.
MINTMINT-4109628.
STRING10090.ENSMUSP00000075279.

PTM databases

PhosphoSiteQ61468.

Proteomic databases

PaxDbQ61468.
PRIDEQ61468.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075884; ENSMUSP00000075279; ENSMUSG00000063011.
GeneID56047.
KEGGmmu:56047.
UCSCuc008bbo.1. mouse.

Organism-specific databases

CTD10232.
MGIMGI:1888992. Msln.

Phylogenomic databases

eggNOGNOG40535.
GeneTreeENSGT00530000064019.
HOGENOMHOG000113666.
HOVERGENHBG081988.
InParanoidQ61468.
OMACDLPGRF.
OrthoDBEOG7M3HZW.
PhylomeDBQ61468.
TreeFamTF331713.

Gene expression databases

BgeeQ61468.
CleanExMM_MSLN.
GenevestigatorQ61468.

Family and domain databases

InterProIPR010335. Mesothelin.
IPR026664. Stereocilin_related.
[Graphical view]
PANTHERPTHR23412. PTHR23412. 1 hit.
PfamPF06060. Mesothelin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311824.
PROQ61468.
SOURCESearch...

Entry information

Entry nameMSLN_MOUSE
AccessionPrimary (citable) accession number: Q61468
Entry history
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot