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Q61464 (ZN638_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 638
Alternative name(s):
Nuclear protein 220
Zinc finger matrin-like protein
Gene names
Name:Znf638
Synonyms:Np220, Zfml
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1960 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Early regulator of adipogenesis that works as a transcription cofactor of CEBPs, controlling the expression of PPARG and probably of other proadipogenic genes, such as SREBF1. Binds to cytidine clusters in double-stranded DNA. Ref.5

Subunit structure

Interacts with FHL2 By similarity. Interacts with CEBPA, CEBPD and CEBPG. Ref.5

Subcellular location

Nucleus speckle Ref.5.

Developmental stage

In an vitro adipocyte differentiation system, induced at the protein and RNA levels shortly after exposure to the induction mixture. Levels peak before PPARG induction and rapidly decrease during later stages of differentiation. Ref.5

Sequence similarities

Contains 1 matrin-type zinc finger.

Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q61464-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61464-2)

The sequence of this isoform differs from the canonical sequence as follows:
     810-832: Missing.
     1147-1200: KEEPKQALCE...SENIDESALN → PGSETVTQKD...GEEAFQMSEG
     1201-1832: Missing.
     1833-1866: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q61464-3)

The sequence of this isoform differs from the canonical sequence as follows:
     438-454: DWIQHQNTSTHIESCRQ → VSVFKRLLYNDAQCPGF
     456-1960: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q61464-4)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1833-1866: Missing.
Isoform 5 (identifier: Q61464-5)

Also known as: Gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     1147-1832: Missing.
Isoform 6 (identifier: Q61464-7)

Also known as: Delta;

The sequence of this isoform differs from the canonical sequence as follows:
     1147-1866: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19601960Zinc finger protein 638
PRO_0000082012

Regions

Domain676 – 75176RRM 1
Domain902 – 97675RRM 2
Zinc finger1876 – 190631Matrin-type
Compositional bias470 – 575106Arg-rich
Compositional bias1577 – 15826Poly-Lys

Amino acid modifications

Modified residue1281Phosphoserine By similarity
Modified residue3811Phosphoserine By similarity
Modified residue4181Phosphoserine By similarity
Modified residue5541Phosphoserine By similarity
Modified residue6061Phosphoserine By similarity
Modified residue6151Phosphoserine By similarity
Modified residue10991Phosphoserine By similarity
Modified residue14001Phosphoserine By similarity
Modified residue16611Phosphoserine By similarity
Modified residue18641Phosphoserine By similarity

Natural variations

Alternative sequence438 – 45417DWIQH…ESCRQ → VSVFKRLLYNDAQCPGF in isoform 3.
VSP_014807
Alternative sequence456 – 19601505Missing in isoform 3.
VSP_014808
Alternative sequence810 – 83223Missing in isoform 2.
VSP_014809
Alternative sequence1147 – 1866720Missing in isoform 6.
VSP_014810
Alternative sequence1147 – 1832686Missing in isoform 5.
VSP_014811
Alternative sequence1147 – 120054KEEPK…ESALN → PGSETVTQKDLKTMPERHLA AKTPMKRVRIGKSSPSQKVA EPTKGEEAFQMSEG in isoform 2.
VSP_014812
Alternative sequence1201 – 1832632Missing in isoform 2.
VSP_014813
Alternative sequence1833 – 186634Missing in isoform 2 and isoform 4.
VSP_014814

Experimental info

Sequence conflict321V → L in AAH76615. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha) [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: 27DF6740BFE410ED

FASTA1,960218,134
        10         20         30         40         50         60 
MSRPRFNPRG TFPLQRPRAP NPPGMRPPGP FVRPGSMGLP RFYPAGRARG IPHRFPGHGS 

        70         80         90        100        110        120 
YQNMGPQRMN VQVTQHRTDP RLTKEKLDFP EAQQKKGKPH GSRWDDESHI TPPVEVKQSS 

       130        140        150        160        170        180 
VTQVTEQSPK VQSRYTKESA SSILASFGLS NEDLEELSRY PDEQLTPENM PLILRDIRMR 

       190        200        210        220        230        240 
KMSRRLPNLP SHSRNKETLS NETVSSNVID YGHASKYGYT EDPLEVRIYD PEIPTDEVKN 

       250        260        270        280        290        300 
EFQPQQSISA TVSTPNVICN SVFPGGDMFR QMDFPGESSS QSFFPVESGT KMSGLHISGQ 

       310        320        330        340        350        360 
SVLEPVKSIS QSISQTVSQT TSQSLNPPSM NQVPFTFELD AVLRQQERIS QKSVISSADA 

       370        380        390        400        410        420 
HGGPTESKKD YQSEADLPIR SPFGIVKASW LPKFTQAGAQ KMKRLPTPSM MNDYYAASPR 

       430        440        450        460        470        480 
IFPHLCSLCN VECSHLKDWI QHQNTSTHIE SCRQLRQQYP DWNPEILPSR RNESNRKENE 

       490        500        510        520        530        540 
TPRRRSHSPS PRHSRRSSSG HRIRRSRSPV RYIYRPRSRS PRICHRFISK YRSRSRSRSR 

       550        560        570        580        590        600 
SRSPYRSRNL LRRSPKSYRS ASPERTSRKS VRSDRKKALE DGGQRSVHGT EVTKQKHTET 

       610        620        630        640        650        660 
VDKGLSPAQK PKLASGTKPS AKSLSSVKSD SHLGAYSAHK SENLEDDTLP EGKQESGKSA 

       670        680        690        700        710        720 
LAQRKPQKDQ SLSSNSILLV SELPEDGFTE EDIRKAFLPF GKISDVLLVP CRNEAYLEME 

       730        740        750        760        770        780 
LRKAVTSIMK YIETMPLVIK GKSVKVCVPG KKKPQNKEMK KKPSDIKKSS ASALKKETDA 

       790        800        810        820        830        840 
SKTMETVSSS SSAKSGQIKS STVKVNKCAG KSAGSVKSVV TVAAKGKASI KTAKSGKKSL 

       850        860        870        880        890        900 
EAKKSGNIKN KDSNKPVTVP ANSEIKASSE DKATGKSAEE SPSGTLEATE KEPVNKESEE 

       910        920        930        940        950        960 
MSVVFISNLP NKGYSTEEIY NLAKPFGALK DILVLSSHKK AYIEINKKSA DSMVKFYTCF 

       970        980        990       1000       1010       1020 
PISMDGNQLS ISMAPEHVDL KDEEALFTTL IQENDPEANI DKIYNRFVHL DNLPEDGLQC 

      1030       1040       1050       1060       1070       1080 
VLCVGHQFGK VDRYMFMSNK NKVILQLESP ESALSMYNFL KQNPQNIGEH VLTCTLSPKT 

      1090       1100       1110       1120       1130       1140 
DSEVQRKNDL ELGKGSTFSP DLKNSPVDES EVQTAADSSS VKPSEVEEET TSNIGTETSV 

      1150       1160       1170       1180       1190       1200 
HQEELGKEEP KQALCESDFA IQTLELEAQG AEVSIEIPLV ASTPANIELF SENIDESALN 

      1210       1220       1230       1240       1250       1260 
QQMYTSDFEK EEAEVTNPET ELAVSDSVFI EERNIKGIIE DSPSETEDIF SGIVQPMVDA 

      1270       1280       1290       1300       1310       1320 
IAEVDKHETV SEVLPSACNV TQAPGSYIED EKVVSKKDIA EKVILDEKEE DEFNVKETRM 

      1330       1340       1350       1360       1370       1380 
DLQVKTEKAE KNEAIIFKEK LEKIIAAIRE KPIESSVIKA DPTKGLDQTS KPDETGKSSV 

      1390       1400       1410       1420       1430       1440 
LTVSNVYSSK SSIKATVVSS PKAKSTPSKT ESHSTFPKPV LREQIKADKK VSAKEFGLLK 

      1450       1460       1470       1480       1490       1500 
NTRSGLAESN SKSKPTQIGV NRGCSGRISA LQCKDSKVDY KDITKQSQET ETKPPIMKRD 

      1510       1520       1530       1540       1550       1560 
DSNNKALALQ NTKNSKSTTD RSSKSKEEPL FTFNLDEFVT VDEVIEEVNP SQAKQNPLKG 

      1570       1580       1590       1600       1610       1620 
KRKEALKISP SPELNLKKKK GKTSVPHSVE GELSFVTLDE IGEEEDATVQ ALVTVDEVID 

      1630       1640       1650       1660       1670       1680 
EEELNMEEMV KNSNSLLTLD ELIDQDDCIP HSGPKDVTVL SMAEEQDLQQ ERLVTVDEIG 

      1690       1700       1710       1720       1730       1740 
EVEESADITF ATLNAKRDKR DSIGFISSQM PEDPSTLVTV DEIQDDSSDF HLMTLDEVTE 

      1750       1760       1770       1780       1790       1800 
EDENSLADFN NLKEELNFVT VDEVGDEEDG DNDSKVELAR GKIEHHTDKK GNRKRRAVDP 

      1810       1820       1830       1840       1850       1860 
KKSKLDSFSQ VGPGSETVTQ KDLKTMPERH LAAKTPMKRV RLGKSSPSQK VAEPTKGEEA 

      1870       1880       1890       1900       1910       1920 
FQMSEGVDDA ELKDSEPDEK RRKTQDSSVG KSMTSDVPGD LDFLVPKAGF FCPICSLFYS 

      1930       1940       1950       1960 
GEKAMANHCK STRHKQNTEK FMAKQRKEKE QNETEERSSR 

« Hide

Isoform 2 [UniParc].

Checksum: 342F546EFC525823
Show »

FASTA1,271142,198
Isoform 3 [UniParc].

Checksum: 5B605A1F5153B1C3
Show »

FASTA45550,951
Isoform 4 (Beta) [UniParc].

Checksum: 3B2F3A7AF4580259
Show »

FASTA1,926214,460
Isoform 5 (Gamma) [UniParc].

Checksum: 344E096A235A8168
Show »

FASTA1,274142,134
Isoform 6 (Delta) [UniParc].

Checksum: F2BF9C3AA2F2883B
Show »

FASTA1,240138,461

References

« Hide 'large scale' references
[1]"A family of novel DNA-binding nuclear proteins having polypyrimidine tract-binding motif and arginine/serine-rich motif."
Matsushima Y., Ohshima M., Sonoda M., Kitagawa Y.
Biochem. Biophys. Res. Commun. 223:427-433(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4; 5 AND 6).
Tissue: Heart.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: C57BL/6J.
Tissue: Cerebellum.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Head.
[4]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 761-768; 873-891; 1395-1404; 1506-1513 AND 1874-1881, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[5]"Regulation of adipocyte differentiation by the zinc finger protein ZNF638."
Meruvu S., Hugendubler L., Mueller E.
J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CEBPA; CEBPD AND CEBPG, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83033 mRNA. Translation: BAA11749.1.
AK043029 mRNA. Translation: BAC31439.1.
BC076615 mRNA. Translation: AAH76615.1.
CCDSCCDS20288.1. [Q61464-4]
CCDS51823.1. [Q61464-2]
PIRJC4842.
RefSeqNP_001159843.1. NM_001166371.1. [Q61464-2]
NP_032743.2. NM_008717.3.
UniGeneMm.132392.

3D structure databases

ProteinModelPortalQ61464.
SMRQ61464. Positions 668-754.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201815. 1 interaction.
IntActQ61464. 1 interaction.
MINTMINT-4109575.

PTM databases

PhosphoSiteQ61464.

Proteomic databases

MaxQBQ61464.
PaxDbQ61464.
PRIDEQ61464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000113835; ENSMUSP00000109466; ENSMUSG00000030016. [Q61464-2]
GeneID18139.
KEGGmmu:18139.
UCSCuc009coo.2. mouse. [Q61464-3]
uc009coq.2. mouse. [Q61464-2]

Organism-specific databases

CTD18139.
MGIMGI:1203484. Zfml.

Phylogenomic databases

eggNOGNOG45231.
GeneTreeENSGT00730000111236.
HOGENOMHOG000082488.
HOVERGENHBG094186.
InParanoidQ61464.
PhylomeDBQ61464.

Gene expression databases

ArrayExpressQ61464.
BgeeQ61464.
CleanExMM_ZFML.
GenevestigatorQ61464.

Family and domain databases

Gene3D3.30.70.330. 3 hits.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
SMARTSM00360. RRM. 2 hits.
SM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
PS50171. ZF_MATRIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZNF638. mouse.
NextBio293380.
PROQ61464.
SOURCESearch...

Entry information

Entry nameZN638_MOUSE
AccessionPrimary (citable) accession number: Q61464
Secondary accession number(s): Q6DFV9, Q8C941
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot