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Q61464

- ZN638_MOUSE

UniProt

Q61464 - ZN638_MOUSE

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Protein

Zinc finger protein 638

Gene
Znf638, Np220, Zfml
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Early regulator of adipogenesis that works as a transcription cofactor of CEBPs, controlling the expression of PPARG and probably of other proadipogenic genes, such as SREBF1. Binds to cytidine clusters in double-stranded DNA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1876 – 190631Matrin-typeAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. nucleotide binding Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 638
Alternative name(s):
Nuclear protein 220
Zinc finger matrin-like protein
Gene namesi
Name:Znf638
Synonyms:Np220, Zfml
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1203484. Zfml.

Subcellular locationi

Nucleus speckle 1 Publication

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19601960Zinc finger protein 638PRO_0000082012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity
Modified residuei381 – 3811Phosphoserine By similarity
Modified residuei418 – 4181Phosphoserine By similarity
Modified residuei554 – 5541Phosphoserine By similarity
Modified residuei606 – 6061Phosphoserine By similarity
Modified residuei615 – 6151Phosphoserine By similarity
Modified residuei1099 – 10991Phosphoserine By similarity
Modified residuei1400 – 14001Phosphoserine By similarity
Modified residuei1661 – 16611Phosphoserine By similarity
Modified residuei1864 – 18641Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ61464.
PaxDbiQ61464.
PRIDEiQ61464.

PTM databases

PhosphoSiteiQ61464.

Expressioni

Developmental stagei

In an vitro adipocyte differentiation system, induced at the protein and RNA levels shortly after exposure to the induction mixture. Levels peak before PPARG induction and rapidly decrease during later stages of differentiation.1 Publication

Gene expression databases

ArrayExpressiQ61464.
BgeeiQ61464.
CleanExiMM_ZFML.
GenevestigatoriQ61464.

Interactioni

Subunit structurei

Interacts with FHL2 By similarity. Interacts with CEBPA, CEBPD and CEBPG.1 Publication

Protein-protein interaction databases

BioGridi201815. 1 interaction.
IntActiQ61464. 1 interaction.
MINTiMINT-4109575.

Structurei

3D structure databases

ProteinModelPortaliQ61464.
SMRiQ61464. Positions 668-754.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini676 – 75176RRM 1Add
BLAST
Domaini902 – 97675RRM 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi470 – 575106Arg-richAdd
BLAST
Compositional biasi1577 – 15826Poly-Lys

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG45231.
GeneTreeiENSGT00730000111236.
HOGENOMiHOG000082488.
HOVERGENiHBG094186.
InParanoidiQ61464.
PhylomeDBiQ61464.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
SM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q61464-1) [UniParc]FASTAAdd to Basket

Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSRPRFNPRG TFPLQRPRAP NPPGMRPPGP FVRPGSMGLP RFYPAGRARG     50
IPHRFPGHGS YQNMGPQRMN VQVTQHRTDP RLTKEKLDFP EAQQKKGKPH 100
GSRWDDESHI TPPVEVKQSS VTQVTEQSPK VQSRYTKESA SSILASFGLS 150
NEDLEELSRY PDEQLTPENM PLILRDIRMR KMSRRLPNLP SHSRNKETLS 200
NETVSSNVID YGHASKYGYT EDPLEVRIYD PEIPTDEVKN EFQPQQSISA 250
TVSTPNVICN SVFPGGDMFR QMDFPGESSS QSFFPVESGT KMSGLHISGQ 300
SVLEPVKSIS QSISQTVSQT TSQSLNPPSM NQVPFTFELD AVLRQQERIS 350
QKSVISSADA HGGPTESKKD YQSEADLPIR SPFGIVKASW LPKFTQAGAQ 400
KMKRLPTPSM MNDYYAASPR IFPHLCSLCN VECSHLKDWI QHQNTSTHIE 450
SCRQLRQQYP DWNPEILPSR RNESNRKENE TPRRRSHSPS PRHSRRSSSG 500
HRIRRSRSPV RYIYRPRSRS PRICHRFISK YRSRSRSRSR SRSPYRSRNL 550
LRRSPKSYRS ASPERTSRKS VRSDRKKALE DGGQRSVHGT EVTKQKHTET 600
VDKGLSPAQK PKLASGTKPS AKSLSSVKSD SHLGAYSAHK SENLEDDTLP 650
EGKQESGKSA LAQRKPQKDQ SLSSNSILLV SELPEDGFTE EDIRKAFLPF 700
GKISDVLLVP CRNEAYLEME LRKAVTSIMK YIETMPLVIK GKSVKVCVPG 750
KKKPQNKEMK KKPSDIKKSS ASALKKETDA SKTMETVSSS SSAKSGQIKS 800
STVKVNKCAG KSAGSVKSVV TVAAKGKASI KTAKSGKKSL EAKKSGNIKN 850
KDSNKPVTVP ANSEIKASSE DKATGKSAEE SPSGTLEATE KEPVNKESEE 900
MSVVFISNLP NKGYSTEEIY NLAKPFGALK DILVLSSHKK AYIEINKKSA 950
DSMVKFYTCF PISMDGNQLS ISMAPEHVDL KDEEALFTTL IQENDPEANI 1000
DKIYNRFVHL DNLPEDGLQC VLCVGHQFGK VDRYMFMSNK NKVILQLESP 1050
ESALSMYNFL KQNPQNIGEH VLTCTLSPKT DSEVQRKNDL ELGKGSTFSP 1100
DLKNSPVDES EVQTAADSSS VKPSEVEEET TSNIGTETSV HQEELGKEEP 1150
KQALCESDFA IQTLELEAQG AEVSIEIPLV ASTPANIELF SENIDESALN 1200
QQMYTSDFEK EEAEVTNPET ELAVSDSVFI EERNIKGIIE DSPSETEDIF 1250
SGIVQPMVDA IAEVDKHETV SEVLPSACNV TQAPGSYIED EKVVSKKDIA 1300
EKVILDEKEE DEFNVKETRM DLQVKTEKAE KNEAIIFKEK LEKIIAAIRE 1350
KPIESSVIKA DPTKGLDQTS KPDETGKSSV LTVSNVYSSK SSIKATVVSS 1400
PKAKSTPSKT ESHSTFPKPV LREQIKADKK VSAKEFGLLK NTRSGLAESN 1450
SKSKPTQIGV NRGCSGRISA LQCKDSKVDY KDITKQSQET ETKPPIMKRD 1500
DSNNKALALQ NTKNSKSTTD RSSKSKEEPL FTFNLDEFVT VDEVIEEVNP 1550
SQAKQNPLKG KRKEALKISP SPELNLKKKK GKTSVPHSVE GELSFVTLDE 1600
IGEEEDATVQ ALVTVDEVID EEELNMEEMV KNSNSLLTLD ELIDQDDCIP 1650
HSGPKDVTVL SMAEEQDLQQ ERLVTVDEIG EVEESADITF ATLNAKRDKR 1700
DSIGFISSQM PEDPSTLVTV DEIQDDSSDF HLMTLDEVTE EDENSLADFN 1750
NLKEELNFVT VDEVGDEEDG DNDSKVELAR GKIEHHTDKK GNRKRRAVDP 1800
KKSKLDSFSQ VGPGSETVTQ KDLKTMPERH LAAKTPMKRV RLGKSSPSQK 1850
VAEPTKGEEA FQMSEGVDDA ELKDSEPDEK RRKTQDSSVG KSMTSDVPGD 1900
LDFLVPKAGF FCPICSLFYS GEKAMANHCK STRHKQNTEK FMAKQRKEKE 1950
QNETEERSSR 1960
Length:1,960
Mass (Da):218,134
Last modified:July 19, 2005 - v2
Checksum:i27DF6740BFE410ED
GO
Isoform 2 (identifier: Q61464-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     810-832: Missing.
     1147-1200: KEEPKQALCE...SENIDESALN → PGSETVTQKD...GEEAFQMSEG
     1201-1832: Missing.
     1833-1866: Missing.

Note: No experimental confirmation available.

Show »
Length:1,271
Mass (Da):142,198
Checksum:i342F546EFC525823
GO
Isoform 3 (identifier: Q61464-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     438-454: DWIQHQNTSTHIESCRQ → VSVFKRLLYNDAQCPGF
     456-1960: Missing.

Note: No experimental confirmation available.

Show »
Length:455
Mass (Da):50,951
Checksum:i5B605A1F5153B1C3
GO
Isoform 4 (identifier: Q61464-4) [UniParc]FASTAAdd to Basket

Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1833-1866: Missing.

Show »
Length:1,926
Mass (Da):214,460
Checksum:i3B2F3A7AF4580259
GO
Isoform 5 (identifier: Q61464-5) [UniParc]FASTAAdd to Basket

Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1147-1832: Missing.

Show »
Length:1,274
Mass (Da):142,134
Checksum:i344E096A235A8168
GO
Isoform 6 (identifier: Q61464-7) [UniParc]FASTAAdd to Basket

Also known as: Delta

The sequence of this isoform differs from the canonical sequence as follows:
     1147-1866: Missing.

Show »
Length:1,240
Mass (Da):138,461
Checksum:iF2BF9C3AA2F2883B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei438 – 45417DWIQH…ESCRQ → VSVFKRLLYNDAQCPGF in isoform 3. VSP_014807Add
BLAST
Alternative sequencei456 – 19601505Missing in isoform 3. VSP_014808Add
BLAST
Alternative sequencei810 – 83223Missing in isoform 2. VSP_014809Add
BLAST
Alternative sequencei1147 – 1866720Missing in isoform 6. VSP_014810Add
BLAST
Alternative sequencei1147 – 1832686Missing in isoform 5. VSP_014811Add
BLAST
Alternative sequencei1147 – 120054KEEPK…ESALN → PGSETVTQKDLKTMPERHLA AKTPMKRVRIGKSSPSQKVA EPTKGEEAFQMSEG in isoform 2. VSP_014812Add
BLAST
Alternative sequencei1201 – 1832632Missing in isoform 2. VSP_014813Add
BLAST
Alternative sequencei1833 – 186634Missing in isoform 2 and isoform 4. VSP_014814Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321V → L in AAH76615. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83033 mRNA. Translation: BAA11749.1.
AK043029 mRNA. Translation: BAC31439.1.
BC076615 mRNA. Translation: AAH76615.1.
CCDSiCCDS20288.1. [Q61464-4]
CCDS51823.1. [Q61464-2]
PIRiJC4842.
RefSeqiNP_001159843.1. NM_001166371.1. [Q61464-2]
NP_032743.2. NM_008717.3.
UniGeneiMm.132392.

Genome annotation databases

EnsembliENSMUST00000113835; ENSMUSP00000109466; ENSMUSG00000030016. [Q61464-2]
GeneIDi18139.
KEGGimmu:18139.
UCSCiuc009coo.2. mouse. [Q61464-3]
uc009coq.2. mouse. [Q61464-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83033 mRNA. Translation: BAA11749.1 .
AK043029 mRNA. Translation: BAC31439.1 .
BC076615 mRNA. Translation: AAH76615.1 .
CCDSi CCDS20288.1. [Q61464-4 ]
CCDS51823.1. [Q61464-2 ]
PIRi JC4842.
RefSeqi NP_001159843.1. NM_001166371.1. [Q61464-2 ]
NP_032743.2. NM_008717.3.
UniGenei Mm.132392.

3D structure databases

ProteinModelPortali Q61464.
SMRi Q61464. Positions 668-754.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201815. 1 interaction.
IntActi Q61464. 1 interaction.
MINTi MINT-4109575.

PTM databases

PhosphoSitei Q61464.

Proteomic databases

MaxQBi Q61464.
PaxDbi Q61464.
PRIDEi Q61464.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000113835 ; ENSMUSP00000109466 ; ENSMUSG00000030016 . [Q61464-2 ]
GeneIDi 18139.
KEGGi mmu:18139.
UCSCi uc009coo.2. mouse. [Q61464-3 ]
uc009coq.2. mouse. [Q61464-2 ]

Organism-specific databases

CTDi 18139.
MGIi MGI:1203484. Zfml.

Phylogenomic databases

eggNOGi NOG45231.
GeneTreei ENSGT00730000111236.
HOGENOMi HOG000082488.
HOVERGENi HBG094186.
InParanoidi Q61464.
PhylomeDBi Q61464.

Miscellaneous databases

ChiTaRSi ZNF638. mouse.
NextBioi 293380.
PROi Q61464.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61464.
Bgeei Q61464.
CleanExi MM_ZFML.
Genevestigatori Q61464.

Family and domain databases

Gene3Di 3.30.70.330. 3 hits.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view ]
SMARTi SM00360. RRM. 2 hits.
SM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 2 hits.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
PS50171. ZF_MATRIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A family of novel DNA-binding nuclear proteins having polypyrimidine tract-binding motif and arginine/serine-rich motif."
    Matsushima Y., Ohshima M., Sonoda M., Kitagawa Y.
    Biochem. Biophys. Res. Commun. 223:427-433(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4; 5 AND 6).
    Tissue: Heart.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Head.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 761-768; 873-891; 1395-1404; 1506-1513 AND 1874-1881, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Regulation of adipocyte differentiation by the zinc finger protein ZNF638."
    Meruvu S., Hugendubler L., Mueller E.
    J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CEBPA; CEBPD AND CEBPG, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiZN638_MOUSE
AccessioniPrimary (citable) accession number: Q61464
Secondary accession number(s): Q6DFV9, Q8C941
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi