Reviewed, UniProtKB/Swiss-Prot Q61457 (CCNE1_MOUSE)

Names and origin

Protein names

Recommended name:
    G1/S-specific cyclin-E1

Gene names

Name:	Ccne1
Synonyms:	Ccne

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G1/S (start) transition.
Subunit structure	Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Part of a complex consisting of UHRF2, CDK2 and CCNE1 By similarity. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1.
Subcellular location	Nucleus By similarity.
Tissue specificity	Found in adult spleen, and to a lesser extent in adult testis and brain.
Post-translational modification	Phosphorylation of Thr-393 and Ser-397 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the cyclin family. Cyclin E subfamily.
Sequence caution	The sequence CAA53482.1 differs from that shown. Reason: Frameshift at position 401.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Nucleus
Molecular function	Cyclin
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA replication initiation Inferred from mutant phenotype. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from direct assay. Source: MGI regulation of cell cycle Inferred from direct assay. Source: MGI
Cellular component	cyclin-dependent protein kinase holoenzyme complex Inferred from physical interaction. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	cyclin-dependent protein kinase regulator activity Inferred from direct assay. Source: MGI kinase activity Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cdc2	P11440	2	EBI-643090,EBI-846949
Cdk2	P97377	2	EBI-643090,EBI-847048

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	G1/S-specific cyclin-E1		PRO_0000080450
Amino acid modifications
	Modified residue	56	1	Phosphoserine By similarity
	Modified residue	74	1	Phosphothreonine By similarity
	Modified residue	101	1	Phosphoserine By similarity
	Modified residue	385	1	Phosphoserine By similarity
	Modified residue	393	1	Phosphothreonine By similarity
	Modified residue	397	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	2	1	P → Q in AAH84588. Ref.2
	Sequence conflict	179	1	H → Q in CAA53482. Ref.1
	Sequence conflict	179	1	H → Q in AAH62152. Ref.2
	Sequence conflict	179	1	H → Q in AAH84588. Ref.2
	Sequence conflict	290 – 291	2	LE → FR in AAH62152. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61457-1 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 4916B5D7117ADA69	FASTA	408	46,986
	10 20 30 40 50 60 MPRERDSTDH SNMKEEGGSD LSVRSRKRKA NVAVFLQDPD EEIAKIDKTV KSEDSSQPWD 70 80 90 100 110 120 DNSACVDPCS FIPTPNKEED NELEYPRTAF QPRKIRPPRA SPLPVLNWGN REEVWRIMLN 130 140 150 160 170 180 KEKTYLRDEH FLQRHPLLQA RMRAVLLDWL MEVCEVYKLH RETFYLAQDF FDRYMASQHN 190 200 210 220 230 240 IIKTLLQLIG ISALFIASKL EEIYPPKLHQ FAYVTDGACS GDEILTMELM MMKALKWRLS 250 260 270 280 290 300 PLTIVSWLNV YVQVAYVNDT GEVLMPQYPQ QVFVQIAELL DLCVLDVGCL EFPYGVLAAS 310 320 330 340 350 360 ALYHFSSLEL MQKVSGYQWC DIEKCVKWMV PFAMVIREMG SSKLKHFRGV PMEDSHNIQT 370 380 390 400 HTNSLDLLDK AQAKKAILSE QNRISPPPSV VLTPPPSSKK QSSEQETE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of murine cyclin E." Damjanov I., Shan J., Wang R.F., Damjanov A., Deloia J.A. Biochem. Biophys. Res. Commun. 201:994-1000(1994) [PubMed: 8003041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo and Limb.
[3]	"Cables enhances cdk2 tyrosine 15 phosphorylation by Wee1, inhibits cell growth, and is lost in many human colon and squamous cancers." Wu C.-L., Kirley S.D., Xiao H., Chuang Y., Chung D.C., Zukerberg L.R. Cancer Res. 61:7325-7332(2001) [PubMed: 11585773] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH CDK2; CCNA1 AND CABLES1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75888 mRNA. Translation: CAA53482.1. Frameshift. BC062152 mRNA. Translation: AAH62152.1. BC084588 mRNA. Translation: AAH84588.1. BC106191 mRNA. Translation: AAI06192.1.
IPI	IPI00875841.
PIR	JC2497.
RefSeq	NP_031659.2.
UniGene	Mm.16110
3D structure databases
SMR	Q61457. Positions 101-370.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61457. 3 interactions.
STRING	Q61457.
PTM databases
PhosphoSite	Q61457.
Proteomic databases
PRIDE	Q61457.
Genome annotation databases
Ensembl	ENSMUST00000108023; ENSMUSP00000103658; ENSMUSG00000002068; Mus musculus. [Genome view]
GeneID	12447.
KEGG	mmu:12447.
Organism-specific databases
CTD	12447.
MGI	MGI:88316. Ccne1.
Phylogenomic databases
HOVERGEN	Q61457.
OrthoDB	EOG986BXT.
Gene expression databases
ArrayExpress	Q61457.
Bgee	Q61457.
CleanEx	MM_CCNE1.
Genevestigator	Q61457.
GermOnline	ENSMUSG00000002068. Mus musculus.
Family and domain databases
InterPro	IPR006670. Cyclin. IPR011028. Cyclin-like. IPR014400. Cyclin_A_B_D_E. IPR004367. Cyclin_C. IPR006671. Cyclin_N. IPR013763. Cyclin_related. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 1 hit.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 1 hit. [Graphical view]
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281272.
SOURCE	Search...

Entry information

Entry name

CCNE1_MOUSE

Accession

Primary (citable) accession number: Q61457
Secondary accession number(s): Q05BA1, Q05BA6, Q05BB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 13, 2007
Last modified:	December 15, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)