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Q61457

- CCNE1_MOUSE

UniProt

Q61457 - CCNE1_MOUSE

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Protein
G1/S-specific cyclin-E1
Gene
Ccne1, Ccne
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G1/S (start) transition.

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase regulator activity Source: MGI
  2. kinase activity Source: MGI
  3. protein binding Source: IntAct
  4. protein kinase binding Source: MGI

GO - Biological processi

  1. DNA replication initiation Source: MGI
  2. G1/S transition of mitotic cell cycle Source: InterPro
  3. Wnt signaling pathway Source: MGI
  4. cell division Source: UniProtKB-KW
  5. protein phosphorylation Source: MGI
  6. regulation of cell cycle Source: MGI
  7. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiREACT_188819. DNA Damage/Telomere Stress Induced Senescence.
REACT_198608. G1/S-Specific Transcription.
REACT_199110. G0 and Early G1.
REACT_206830. E2F mediated regulation of DNA replication.
REACT_219010. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.

Names & Taxonomyi

Protein namesi
Recommended name:
G1/S-specific cyclin-E1
Gene namesi
Name:Ccne1
Synonyms:Ccne
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:88316. Ccne1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408G1/S-specific cyclin-E1
PRO_0000080450Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei74 – 741Phosphothreonine By similarity
Modified residuei101 – 1011Phosphoserine By similarity
Modified residuei385 – 3851Phosphoserine By similarity
Modified residuei393 – 3931Phosphothreonine By similarity
Modified residuei397 – 3971Phosphoserine By similarity

Post-translational modificationi

Phosphorylation of both Thr-393 by GSK3 and Ser-397 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-74 creates a low affinity degron also recognized by FBXW7 By similarity.
Ubiquitinated by UHRF2; appears to occur independently of phosphorylation By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ61457.

PTM databases

PhosphoSiteiQ61457.

Expressioni

Tissue specificityi

Found in adult spleen, and to a lesser extent in adult testis and brain.

Gene expression databases

ArrayExpressiQ61457.
BgeeiQ61457.
CleanExiMM_CCNE1.
GenevestigatoriQ61457.

Interactioni

Subunit structurei

Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation By similarity. Found in a complex with CDK2, CABLES1 and CCNA1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdk1P114402EBI-643090,EBI-846949
Cdk2P973773EBI-643090,EBI-847048

Protein-protein interaction databases

BioGridi198552. 11 interactions.
DIPiDIP-45868N.
IntActiQ61457. 3 interactions.
MINTiMINT-1706339.

Structurei

3D structure databases

ProteinModelPortaliQ61457.
SMRiQ61457. Positions 101-370.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5024.
GeneTreeiENSGT00750000117276.
HOGENOMiHOG000231743.
HOVERGENiHBG050834.
KOiK06626.
OMAiPLLQPKM.
OrthoDBiEOG7HB595.
PhylomeDBiQ61457.
TreeFamiTF101005.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR028858. Cyclin_E.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERiPTHR10177:SF71. PTHR10177:SF71. 1 hit.
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61457-1 [UniParc]FASTAAdd to Basket

« Hide

MPRERDSTDH SNMKEEGGSD LSVRSRKRKA NVAVFLQDPD EEIAKIDKTV    50
KSEDSSQPWD DNSACVDPCS FIPTPNKEED NELEYPRTAF QPRKIRPPRA 100
SPLPVLNWGN REEVWRIMLN KEKTYLRDEH FLQRHPLLQA RMRAVLLDWL 150
MEVCEVYKLH RETFYLAQDF FDRYMASQHN IIKTLLQLIG ISALFIASKL 200
EEIYPPKLHQ FAYVTDGACS GDEILTMELM MMKALKWRLS PLTIVSWLNV 250
YVQVAYVNDT GEVLMPQYPQ QVFVQIAELL DLCVLDVGCL EFPYGVLAAS 300
ALYHFSSLEL MQKVSGYQWC DIEKCVKWMV PFAMVIREMG SSKLKHFRGV 350
PMEDSHNIQT HTNSLDLLDK AQAKKAILSE QNRISPPPSV VLTPPPSSKK 400
QSSEQETE 408
Length:408
Mass (Da):46,986
Last modified:November 13, 2007 - v2
Checksum:i4916B5D7117ADA69
GO

Sequence cautioni

The sequence CAA53482.1 differs from that shown. Reason: Frameshift at position 401.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21P → Q in AAH84588. 1 Publication
Sequence conflicti179 – 1791H → Q in CAA53482. 1 Publication
Sequence conflicti179 – 1791H → Q in AAH62152. 1 Publication
Sequence conflicti179 – 1791H → Q in AAH84588. 1 Publication
Sequence conflicti290 – 2912LE → FR in AAH62152. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75888 mRNA. Translation: CAA53482.1. Frameshift.
BC062152 mRNA. Translation: AAH62152.1.
BC084588 mRNA. Translation: AAH84588.1.
BC106191 mRNA. Translation: AAI06192.1.
CCDSiCCDS39914.1.
PIRiJC2497.
RefSeqiNP_031659.2. NM_007633.2.
UniGeneiMm.16110.

Genome annotation databases

EnsembliENSMUST00000108023; ENSMUSP00000103658; ENSMUSG00000002068.
GeneIDi12447.
KEGGimmu:12447.
UCSCiuc009gkr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75888 mRNA. Translation: CAA53482.1 . Frameshift.
BC062152 mRNA. Translation: AAH62152.1 .
BC084588 mRNA. Translation: AAH84588.1 .
BC106191 mRNA. Translation: AAI06192.1 .
CCDSi CCDS39914.1.
PIRi JC2497.
RefSeqi NP_031659.2. NM_007633.2.
UniGenei Mm.16110.

3D structure databases

ProteinModelPortali Q61457.
SMRi Q61457. Positions 101-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198552. 11 interactions.
DIPi DIP-45868N.
IntActi Q61457. 3 interactions.
MINTi MINT-1706339.

PTM databases

PhosphoSitei Q61457.

Proteomic databases

PRIDEi Q61457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000108023 ; ENSMUSP00000103658 ; ENSMUSG00000002068 .
GeneIDi 12447.
KEGGi mmu:12447.
UCSCi uc009gkr.1. mouse.

Organism-specific databases

CTDi 898.
MGIi MGI:88316. Ccne1.

Phylogenomic databases

eggNOGi COG5024.
GeneTreei ENSGT00750000117276.
HOGENOMi HOG000231743.
HOVERGENi HBG050834.
KOi K06626.
OMAi PLLQPKM.
OrthoDBi EOG7HB595.
PhylomeDBi Q61457.
TreeFami TF101005.

Enzyme and pathway databases

Reactomei REACT_188819. DNA Damage/Telomere Stress Induced Senescence.
REACT_198608. G1/S-Specific Transcription.
REACT_199110. G0 and Early G1.
REACT_206830. E2F mediated regulation of DNA replication.
REACT_219010. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.

Miscellaneous databases

ChiTaRSi CCNE1. mouse.
NextBioi 281272.
PROi Q61457.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61457.
Bgeei Q61457.
CleanExi MM_CCNE1.
Genevestigatori Q61457.

Family and domain databases

Gene3Di 1.10.472.10. 2 hits.
InterProi IPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR028858. Cyclin_E.
IPR006671. Cyclin_N.
[Graphical view ]
PANTHERi PTHR10177:SF71. PTHR10177:SF71. 1 hit.
Pfami PF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTi SM00385. CYCLIN. 1 hit.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 2 hits.
PROSITEi PS00292. CYCLINS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo and Limb.
  3. "Cables enhances cdk2 tyrosine 15 phosphorylation by Wee1, inhibits cell growth, and is lost in many human colon and squamous cancers."
    Wu C.-L., Kirley S.D., Xiao H., Chuang Y., Chung D.C., Zukerberg L.R.
    Cancer Res. 61:7325-7332(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH CDK2; CCNA1 AND CABLES1.

Entry informationi

Entry nameiCCNE1_MOUSE
AccessioniPrimary (citable) accession number: Q61457
Secondary accession number(s): Q05BA1, Q05BA6, Q05BB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 13, 2007
Last modified: September 3, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi