G1/S-specific cyclin-E1 - Mus musculus (Mouse)

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Q61457 (CCNE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
G1/S-specific cyclin-E1

Gene names

Name:	Ccne1
Synonyms:	Ccne

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G1/S (start) transition.
Subunit structure	Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Part of a complex consisting of UHRF2, CDK2 and CCNE1 By similarity. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1.
Subcellular location	Nucleus By similarity.
Tissue specificity	Found in adult spleen, and to a lesser extent in adult testis and brain.
Post-translational modification	Phosphorylation of Thr-393 and Ser-397 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the cyclin family. Cyclin E subfamily.
Sequence caution	The sequence CAA53482.1 differs from that shown. Reason: Frameshift at position 401.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Nucleus
Molecular function	Cyclin
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from mutant phenotype. Source: MGI Wnt receptor signaling pathway Inferred from direct assay. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein phosphorylation Inferred from direct assay. Source: MGI regulation of cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: InterPro
Cellular component	cyclin-dependent protein kinase holoenzyme complex Inferred from physical interaction. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	cyclin-dependent protein kinase regulator activity Inferred from direct assay. Source: MGI kinase activity Inferred from direct assay. Source: MGI protein kinase binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cdk1	P11440	2	EBI-643090,EBI-846949
Cdk2	P97377	2	EBI-643090,EBI-847048

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 408

408

G1/S-specific cyclin-E1

PRO_0000080450

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphothreonine By similarity

Modified residue

101

Phosphoserine By similarity

Modified residue

379

Phosphoserine By similarity

Modified residue

385

Phosphoserine By similarity

Modified residue

393

Phosphothreonine By similarity

Modified residue

397

Phosphoserine By similarity

Experimental info

Sequence conflict

P → Q in AAH84588. Ref.2

Sequence conflict

179

H → Q in CAA53482. Ref.1

Sequence conflict

179

H → Q in AAH62152. Ref.2

Sequence conflict

179

H → Q in AAH84588. Ref.2

Sequence conflict

290 – 291

LE → FR in AAH62152. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q61457 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: 4916B5D7117ADA69
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FASTA

408

46,986

        10         20         30         40         50         60 
MPRERDSTDH SNMKEEGGSD LSVRSRKRKA NVAVFLQDPD EEIAKIDKTV KSEDSSQPWD 

        70         80         90        100        110        120 
DNSACVDPCS FIPTPNKEED NELEYPRTAF QPRKIRPPRA SPLPVLNWGN REEVWRIMLN 

       130        140        150        160        170        180 
KEKTYLRDEH FLQRHPLLQA RMRAVLLDWL MEVCEVYKLH RETFYLAQDF FDRYMASQHN 

       190        200        210        220        230        240 
IIKTLLQLIG ISALFIASKL EEIYPPKLHQ FAYVTDGACS GDEILTMELM MMKALKWRLS 

       250        260        270        280        290        300 
PLTIVSWLNV YVQVAYVNDT GEVLMPQYPQ QVFVQIAELL DLCVLDVGCL EFPYGVLAAS 

       310        320        330        340        350        360 
ALYHFSSLEL MQKVSGYQWC DIEKCVKWMV PFAMVIREMG SSKLKHFRGV PMEDSHNIQT 

       370        380        390        400 
HTNSLDLLDK AQAKKAILSE QNRISPPPSV VLTPPPSSKK QSSEQETE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of murine cyclin E." Damjanov I., Shan J., Wang R.F., Damjanov A., Deloia J.A. Biochem. Biophys. Res. Commun. 201:994-1000(1994) [PubMed: 8003041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo and Limb.
[3]	"Cables enhances cdk2 tyrosine 15 phosphorylation by Wee1, inhibits cell growth, and is lost in many human colon and squamous cancers." Wu C.-L., Kirley S.D., Xiao H., Chuang Y., Chung D.C., Zukerberg L.R. Cancer Res. 61:7325-7332(2001) [PubMed: 11585773] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH CDK2; CCNA1 AND CABLES1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75888 mRNA. Translation: CAA53482.1. Frameshift. BC062152 mRNA. Translation: AAH62152.1. BC084588 mRNA. Translation: AAH84588.1. BC106191 mRNA. Translation: AAI06192.1.
IPI	IPI00121227.
PIR	JC2497.
RefSeq	NP_031659.2. NM_007633.2.
UniGene	Mm.16110.
3D structure databases
ProteinModelPortal	Q61457.
SMR	Q61457. Positions 101-370.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61457. 3 interactions.
STRING	Q61457.
PTM databases
PhosphoSite	Q61457.
Proteomic databases
PRIDE	Q61457.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000108023; ENSMUSP00000103658; ENSMUSG00000002068.
GeneID	12447.
KEGG	mmu:12447.
UCSC	uc009gkr.1. mouse.
Organism-specific databases
CTD	898.
MGI	MGI:88316. Ccne1.
Phylogenomic databases
GeneTree	ENSGT00560000076942.
HOVERGEN	HBG050834.
OrthoDB	EOG45756T.
Gene expression databases
ArrayExpress	Q61457.
Bgee	Q61457.
CleanEx	MM_CCNE1.
Genevestigator	Q61457.
GermOnline	ENSMUSG00000002068. Mus musculus.
Family and domain databases
InterPro	IPR013763. Cyclin-like. IPR014400. Cyclin_A/B/D/E. IPR004367. Cyclin_C. IPR006671. Cyclin_N. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 2 hits.
KO	K06626.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 1 hit. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 1 hit.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281272.
SOURCE	Search...

Entry information

Entry name

CCNE1_MOUSE

Accession

Primary (citable) accession number: Q61457
Secondary accession number(s): Q05BA1, Q05BA6, Q05BB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 13, 2007
Last modified:	November 16, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents