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Q61457

- CCNE1_MOUSE

UniProt

Q61457 - CCNE1_MOUSE

Protein

G1/S-specific cyclin-E1

Gene

Ccne1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Essential for the control of the cell cycle at the G1/S (start) transition.

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase regulator activity Source: MGI
    2. kinase activity Source: MGI
    3. protein binding Source: IntAct
    4. protein kinase binding Source: MGI

    GO - Biological processi

    1. cell division Source: UniProtKB-KW
    2. DNA replication initiation Source: MGI
    3. G1/S transition of mitotic cell cycle Source: InterPro
    4. protein phosphorylation Source: MGI
    5. regulation of cell cycle Source: MGI
    6. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
    7. Wnt signaling pathway Source: MGI

    Keywords - Molecular functioni

    Cyclin

    Keywords - Biological processi

    Cell cycle, Cell division

    Enzyme and pathway databases

    ReactomeiREACT_188819. DNA Damage/Telomere Stress Induced Senescence.
    REACT_198608. G1/S-Specific Transcription.
    REACT_199110. G0 and Early G1.
    REACT_206830. E2F mediated regulation of DNA replication.
    REACT_219010. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
    REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G1/S-specific cyclin-E1
    Gene namesi
    Name:Ccne1
    Synonyms:Ccne
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:88316. Ccne1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 408408G1/S-specific cyclin-E1PRO_0000080450Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei74 – 741PhosphothreonineBy similarity
    Modified residuei101 – 1011PhosphoserineBy similarity
    Modified residuei385 – 3851PhosphoserineBy similarity
    Modified residuei393 – 3931PhosphothreonineBy similarity
    Modified residuei397 – 3971PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation of both Thr-393 by GSK3 and Ser-397 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-74 creates a low affinity degron also recognized by FBXW7 By similarity.By similarity
    Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ61457.

    PTM databases

    PhosphoSiteiQ61457.

    Expressioni

    Tissue specificityi

    Found in adult spleen, and to a lesser extent in adult testis and brain.

    Gene expression databases

    ArrayExpressiQ61457.
    BgeeiQ61457.
    CleanExiMM_CCNE1.
    GenevestigatoriQ61457.

    Interactioni

    Subunit structurei

    Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation By similarity. Found in a complex with CDK2, CABLES1 and CCNA1.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cdk1P114402EBI-643090,EBI-846949
    Cdk2P973773EBI-643090,EBI-847048

    Protein-protein interaction databases

    BioGridi198552. 11 interactions.
    DIPiDIP-45868N.
    IntActiQ61457. 3 interactions.
    MINTiMINT-1706339.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61457.
    SMRiQ61457. Positions 101-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cyclin family. Cyclin E subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5024.
    GeneTreeiENSGT00750000117276.
    HOGENOMiHOG000231743.
    HOVERGENiHBG050834.
    KOiK06626.
    OMAiPLLQPKM.
    OrthoDBiEOG7HB595.
    PhylomeDBiQ61457.
    TreeFamiTF101005.

    Family and domain databases

    Gene3Di1.10.472.10. 2 hits.
    InterProiIPR013763. Cyclin-like.
    IPR014400. Cyclin_A/B/D/E/F.
    IPR004367. Cyclin_C-dom.
    IPR028858. Cyclin_E.
    IPR006671. Cyclin_N.
    [Graphical view]
    PANTHERiPTHR10177:SF71. PTHR10177:SF71. 1 hit.
    PfamiPF02984. Cyclin_C. 1 hit.
    PF00134. Cyclin_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
    SMARTiSM00385. CYCLIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.
    PROSITEiPS00292. CYCLINS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRERDSTDH SNMKEEGGSD LSVRSRKRKA NVAVFLQDPD EEIAKIDKTV    50
    KSEDSSQPWD DNSACVDPCS FIPTPNKEED NELEYPRTAF QPRKIRPPRA 100
    SPLPVLNWGN REEVWRIMLN KEKTYLRDEH FLQRHPLLQA RMRAVLLDWL 150
    MEVCEVYKLH RETFYLAQDF FDRYMASQHN IIKTLLQLIG ISALFIASKL 200
    EEIYPPKLHQ FAYVTDGACS GDEILTMELM MMKALKWRLS PLTIVSWLNV 250
    YVQVAYVNDT GEVLMPQYPQ QVFVQIAELL DLCVLDVGCL EFPYGVLAAS 300
    ALYHFSSLEL MQKVSGYQWC DIEKCVKWMV PFAMVIREMG SSKLKHFRGV 350
    PMEDSHNIQT HTNSLDLLDK AQAKKAILSE QNRISPPPSV VLTPPPSSKK 400
    QSSEQETE 408
    Length:408
    Mass (Da):46,986
    Last modified:November 13, 2007 - v2
    Checksum:i4916B5D7117ADA69
    GO

    Sequence cautioni

    The sequence CAA53482.1 differs from that shown. Reason: Frameshift at position 401.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21P → Q in AAH84588. (PubMed:15489334)Curated
    Sequence conflicti179 – 1791H → Q in CAA53482. (PubMed:8003041)Curated
    Sequence conflicti179 – 1791H → Q in AAH62152. (PubMed:15489334)Curated
    Sequence conflicti179 – 1791H → Q in AAH84588. (PubMed:15489334)Curated
    Sequence conflicti290 – 2912LE → FR in AAH62152. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75888 mRNA. Translation: CAA53482.1. Frameshift.
    BC062152 mRNA. Translation: AAH62152.1.
    BC084588 mRNA. Translation: AAH84588.1.
    BC106191 mRNA. Translation: AAI06192.1.
    CCDSiCCDS39914.1.
    PIRiJC2497.
    RefSeqiNP_031659.2. NM_007633.2.
    UniGeneiMm.16110.

    Genome annotation databases

    EnsembliENSMUST00000108023; ENSMUSP00000103658; ENSMUSG00000002068.
    GeneIDi12447.
    KEGGimmu:12447.
    UCSCiuc009gkr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75888 mRNA. Translation: CAA53482.1 . Frameshift.
    BC062152 mRNA. Translation: AAH62152.1 .
    BC084588 mRNA. Translation: AAH84588.1 .
    BC106191 mRNA. Translation: AAI06192.1 .
    CCDSi CCDS39914.1.
    PIRi JC2497.
    RefSeqi NP_031659.2. NM_007633.2.
    UniGenei Mm.16110.

    3D structure databases

    ProteinModelPortali Q61457.
    SMRi Q61457. Positions 101-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198552. 11 interactions.
    DIPi DIP-45868N.
    IntActi Q61457. 3 interactions.
    MINTi MINT-1706339.

    PTM databases

    PhosphoSitei Q61457.

    Proteomic databases

    PRIDEi Q61457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000108023 ; ENSMUSP00000103658 ; ENSMUSG00000002068 .
    GeneIDi 12447.
    KEGGi mmu:12447.
    UCSCi uc009gkr.1. mouse.

    Organism-specific databases

    CTDi 898.
    MGIi MGI:88316. Ccne1.

    Phylogenomic databases

    eggNOGi COG5024.
    GeneTreei ENSGT00750000117276.
    HOGENOMi HOG000231743.
    HOVERGENi HBG050834.
    KOi K06626.
    OMAi PLLQPKM.
    OrthoDBi EOG7HB595.
    PhylomeDBi Q61457.
    TreeFami TF101005.

    Enzyme and pathway databases

    Reactomei REACT_188819. DNA Damage/Telomere Stress Induced Senescence.
    REACT_198608. G1/S-Specific Transcription.
    REACT_199110. G0 and Early G1.
    REACT_206830. E2F mediated regulation of DNA replication.
    REACT_219010. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
    REACT_220647. SCF(Skp2)-mediated degradation of p27/p21.

    Miscellaneous databases

    ChiTaRSi CCNE1. mouse.
    NextBioi 281272.
    PROi Q61457.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61457.
    Bgeei Q61457.
    CleanExi MM_CCNE1.
    Genevestigatori Q61457.

    Family and domain databases

    Gene3Di 1.10.472.10. 2 hits.
    InterProi IPR013763. Cyclin-like.
    IPR014400. Cyclin_A/B/D/E/F.
    IPR004367. Cyclin_C-dom.
    IPR028858. Cyclin_E.
    IPR006671. Cyclin_N.
    [Graphical view ]
    PANTHERi PTHR10177:SF71. PTHR10177:SF71. 1 hit.
    Pfami PF02984. Cyclin_C. 1 hit.
    PF00134. Cyclin_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001771. Cyclin_A_B_D_E. 1 hit.
    SMARTi SM00385. CYCLIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    PROSITEi PS00292. CYCLINS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo and Limb.
    3. "Cables enhances cdk2 tyrosine 15 phosphorylation by Wee1, inhibits cell growth, and is lost in many human colon and squamous cancers."
      Wu C.-L., Kirley S.D., Xiao H., Chuang Y., Chung D.C., Zukerberg L.R.
      Cancer Res. 61:7325-7332(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH CDK2; CCNA1 AND CABLES1.

    Entry informationi

    Entry nameiCCNE1_MOUSE
    AccessioniPrimary (citable) accession number: Q61457
    Secondary accession number(s): Q05BA1, Q05BA6, Q05BB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3