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Q61423

- KCNA4_MOUSE

UniProt

Q61423 - KCNA4_MOUSE

Protein

Potassium voltage-gated channel subfamily A member 4

Gene

Kcna4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. potassium ion binding Source: InterPro
    2. voltage-gated potassium channel activity Source: InterPro

    GO - Biological processi

    1. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199077. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily A member 4
    Alternative name(s):
    Voltage-gated potassium channel subunit Kv1.4
    Gene namesi
    Name:Kcna4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:96661. Kcna4.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: MGI
    2. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 654654Potassium voltage-gated channel subfamily A member 4PRO_0000053982Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
    Modified residuei600 – 6001Phosphoserine; by PKASequence Analysis
    Glycosylationi643 – 6431N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ61423.
    PRIDEiQ61423.

    PTM databases

    PhosphoSiteiQ61423.

    Expressioni

    Gene expression databases

    BgeeiQ61423.
    GenevestigatoriQ61423.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with SIGMAR1 By similarity. Part of a complex containing KCNA1, KCNAB1 and LGI1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200879. 1 interaction.
    IntActiQ61423. 1 interaction.
    MINTiMINT-1780501.
    STRINGi10090.ENSMUSP00000106689.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61423.
    SMRiQ61423. Positions 1-75, 176-572.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei309 – 32719Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei372 – 39322Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei405 – 42521Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei444 – 46219Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei479 – 49820Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei540 – 56223Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi525 – 5306Selectivity filterBy similarity
    Motifi652 – 6543PDZ-bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi38 – 5013Poly-AlaAdd
    BLAST
    Compositional biasi62 – 654Poly-His
    Compositional biasi83 – 897Poly-Arg
    Compositional biasi123 – 13715Poly-GluAdd
    BLAST
    Compositional biasi162 – 1665Poly-Gly
    Compositional biasi434 – 4374Poly-Gln

    Domaini

    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115291.
    HOGENOMiHOG000231015.
    HOVERGENiHBG052230.
    InParanoidiQ8CBF8.
    KOiK04877.
    OMAiKKAHHRQ.
    OrthoDBiEOG7M0NRD.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    1.20.5.600. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR020467. K_chnl_volt-dep_Kv1.4.
    IPR012897. K_chnl_volt-dep_Kv1.4_TID.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF07941. K_channel_TID. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01511. KV14CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q61423-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVAMVSAES SGCNSHMPYG YAAQARARER ERLAHSRAAA AAAVAAATAA    50
    VEGTGGSGGG PHHHHQTRGA YSSHDPQGSR GSRRRRRQRT EKKKLHHRQS 100
    SFPHCSDLMP SGSEEKILRE LSEEEEDEEE EEEEEEEGRF YYSEEDHGDG 150
    CSYTDLLPQD DGGGGGYSSV RYSDCCERVV INVSGLRFET QMKTLAQFPE 200
    TLLGDPEKRT QYFDPLRNEY FFDRNRPSFD AILYYYQSGG RLKRPVNVPF 250
    DIFTEEVKFY QLGEEALLKF REDEGFVREE EDRALPENEF KKQIWLLFEY 300
    PESSSPARGI AIVSVLVILI SIVIFCLETL PEFRDDRDLI MALSAGGHSR 350
    LLNDTSAPHL ENSGHTIFND PFFIVETVCI VWFSFEFVVR CFACPSQALF 400
    FKNIMNIIDI VSILPYFITL GTDLAQQQGG GNGQQQQAMS FAILRIIRLV 450
    RVFRIFKLSR HSKGLQILGH TLRASMRELG LLIFFLFIGV ILFSSAVYFA 500
    EADEPTTHFQ SIPDAFWWAV VTMTTVGYGD MKPITVGGKI VGSLCAIAGV 550
    LTIALPVPVI VSNFNYFYHR ETENEEQTQL TQNAVSCPYL PSNLLKKFRS 600
    STSSSLGDKS EYLEMEEGVK ESLCGKEEKC QGKGDESETD KNNCSNAKAV 650
    ETDV 654
    Length:654
    Mass (Da):73,470
    Last modified:July 27, 2011 - v2
    Checksum:i9322A3DC9CBA2AC4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 1601D → E in AAB60668. (PubMed:8020965)Curated
    Sequence conflicti395 – 3951P → T in AAB60668. (PubMed:8020965)Curated
    Sequence conflicti636 – 6361E → D in AAB60668. (PubMed:8020965)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03723 Genomic DNA. Translation: AAB60668.1.
    AK036112 mRNA. Translation: BAC29309.1.
    BX293548 Genomic DNA. Translation: CAM23761.1.
    CH466519 Genomic DNA. Translation: EDL27774.1.
    BC109014 mRNA. Translation: AAI09015.1.
    CCDSiCCDS16505.1.
    PIRiS09045.
    RefSeqiNP_067250.2. NM_021275.4.
    XP_006498875.1. XM_006498812.1.
    UniGeneiMm.142718.

    Genome annotation databases

    EnsembliENSMUST00000037012; ENSMUSP00000037958; ENSMUSG00000042604.
    GeneIDi16492.
    KEGGimmu:16492.
    UCSCiuc008llu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03723 Genomic DNA. Translation: AAB60668.1 .
    AK036112 mRNA. Translation: BAC29309.1 .
    BX293548 Genomic DNA. Translation: CAM23761.1 .
    CH466519 Genomic DNA. Translation: EDL27774.1 .
    BC109014 mRNA. Translation: AAI09015.1 .
    CCDSi CCDS16505.1.
    PIRi S09045.
    RefSeqi NP_067250.2. NM_021275.4.
    XP_006498875.1. XM_006498812.1.
    UniGenei Mm.142718.

    3D structure databases

    ProteinModelPortali Q61423.
    SMRi Q61423. Positions 1-75, 176-572.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200879. 1 interaction.
    IntActi Q61423. 1 interaction.
    MINTi MINT-1780501.
    STRINGi 10090.ENSMUSP00000106689.

    Chemistry

    GuidetoPHARMACOLOGYi 541.

    PTM databases

    PhosphoSitei Q61423.

    Proteomic databases

    PaxDbi Q61423.
    PRIDEi Q61423.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037012 ; ENSMUSP00000037958 ; ENSMUSG00000042604 .
    GeneIDi 16492.
    KEGGi mmu:16492.
    UCSCi uc008llu.2. mouse.

    Organism-specific databases

    CTDi 3739.
    MGIi MGI:96661. Kcna4.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115291.
    HOGENOMi HOG000231015.
    HOVERGENi HBG052230.
    InParanoidi Q8CBF8.
    KOi K04877.
    OMAi KKAHHRQ.
    OrthoDBi EOG7M0NRD.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199077. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 289795.
    PROi Q61423.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61423.
    Genevestigatori Q61423.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    1.20.5.600. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR020467. K_chnl_volt-dep_Kv1.4.
    IPR012897. K_chnl_volt-dep_Kv1.4_TID.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF07941. K_channel_TID. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01511. KV14CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organization, nucleotide sequence, biophysical properties, and localization of the voltage-gated K+ channel gene KCNA4/Kv1.4 to mouse chromosome 2/human 11p14 and mapping of KCNC1/Kv3.1 to mouse 7/human 11p14.3-p15.2 and KCNA1/Kv1.1 to human 12p13."
      Wymore R.S., Korenberg J.R., Kinoshita K.D., Aiyar J., Coyne C., Chen X.N., Hustad C.M., Copeland N.G., Gutman G.A., Jenkins N.A., Chandy K.G.
      Genomics 20:191-202(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AKR.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiKCNA4_MOUSE
    AccessioniPrimary (citable) accession number: Q61423
    Secondary accession number(s): Q8CBF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3