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Protein

H(+)/Cl(-) exchange transporter 4

Gene

Clcn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei168 – 1681ChlorideBy similarity
Sitei211 – 2111Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity
Sitei268 – 2681Mediates proton transfer from the protein to the inner aqueous phaseBy similarity
Binding sitei456 – 4561Chloride; via amide nitrogenBy similarity
Binding sitei559 – 5591ChlorideBy similarity
Binding sitei597 – 5971ATP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi618 – 6203ATPBy similarity
Nucleotide bindingi725 – 7284ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Chloride, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter 4
Alternative name(s):
Chloride channel protein 4
Short name:
ClC-4
Chloride transporter ClC-4
Gene namesi
Name:Clcn4
Synonyms:Clc4, Clcn4-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104571. Clcn4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5454CytoplasmicBy similarityAdd
BLAST
Transmembranei55 – 9238HelicalBy similarityAdd
BLAST
Transmembranei138 – 16124HelicalBy similarityAdd
BLAST
Intramembranei170 – 1778HelicalBy similarity
Transmembranei187 – 20519HelicalBy similarityAdd
BLAST
Transmembranei211 – 23020HelicalBy similarityAdd
BLAST
Intramembranei242 – 25413HelicalBy similarityAdd
BLAST
Intramembranei258 – 2669HelicalBy similarity
Transmembranei278 – 29619HelicalBy similarityAdd
BLAST
Transmembranei320 – 34526HelicalBy similarityAdd
BLAST
Transmembranei352 – 37221HelicalBy similarityAdd
BLAST
Transmembranei429 – 44921HelicalBy similarityAdd
BLAST
Transmembranei454 – 47320HelicalBy similarityAdd
BLAST
Intramembranei501 – 51515HelicalBy similarityAdd
BLAST
Intramembranei519 – 53012HelicalBy similarityAdd
BLAST
Intramembranei531 – 5344Note=Loop between two helicesBy similarity
Transmembranei535 – 55319HelicalBy similarityAdd
BLAST
Topological domaini554 – 747194CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 747747H(+)/Cl(-) exchange transporter 4PRO_0000094444Add
BLAST

Proteomic databases

EPDiQ61418.
MaxQBiQ61418.
PaxDbiQ61418.
PRIDEiQ61418.

PTM databases

iPTMnetiQ61418.
PhosphoSiteiQ61418.

Expressioni

Gene expression databases

BgeeiQ61418.
CleanExiMM_CLCN4-2.
GenevisibleiQ61418. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000619.

Structurei

3D structure databases

ProteinModelPortaliQ61418.
SMRiQ61418. Positions 135-559, 579-740.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini587 – 65367CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini680 – 74263CBS 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi167 – 1715Selectivity filter part_1By similarity
Motifi209 – 2135Selectivity filter part_2By similarity
Motifi454 – 4585Selectivity filter part_3By similarity

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0475. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
HOVERGENiHBG050984.
InParanoidiQ61418.
KOiK05012.
OMAiRESIWEF.
OrthoDBiEOG7MWGXT.
TreeFamiTF313867.

Family and domain databases

Gene3Di1.10.3080.10. 3 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002246. Cl_channel-4.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01115. CLCHANNEL4.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFLEEPFPD VGTYEDFHTI DWLREKSRDT DRHRKITSKS KESIWEFIKS
60 70 80 90 100
LLDAWSGWVV MLLIGLLAGT LAGVIDLAVD WMTDLKEGVC LSAFWYSHEQ
110 120 130 140 150
CCWTSNETTF EDRDKCPLWQ KWSELLLSQS EGASAYILNY LMYILWALLF
160 170 180 190 200
AFLAVSLVRV FAPYACGSGI PEIKTILSGF IIRGYLGKWT LLIKTVTLVL
210 220 230 240 250
VVSSGLSLGK EGPLVHVACC CGNFFSSLFS KYSKNEGKRR EVLSAAAAAG
260 270 280 290 300
VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN
310 320 330 340 350
SRLVLFYVEY HTPWYMAELF PFILLGVFGG LWGTLFTRCN IAWCRRRKTT
360 370 380 390 400
RLGRYPVLEV IAVTAVTAIV AYPNPYTRQS TSELISELFN DCGALESSQL
410 420 430 440 450
CDYINDPNMT RPVDDIPDRP AGVGVYTAMW QLALALIFKI VITIFTFGMK
460 470 480 490 500
IPSGLFIPSM AVGAMAGRMV GIGVEQLAYH HHDWIIFRNW CRPGADCVTP
510 520 530 540 550
GLYAMVGAAA CLGGVTRMTV SLVVIMFELT GGLEYIVPLM AAAVTSKWVA
560 570 580 590 600
DAFGKEGIYE AHIHLNGYPF LDVKDEFTHR TLATDVMRPR RGEPPLSVLT
610 620 630 640 650
QDSMTVEDVE TLIKETDYNG FPVLVSRDSE RLIGFAQRRE LILAIKNARQ
660 670 680 690 700
RQEGIVSNSI MYFTEEPPEL PANSPHPLKL RRILNLSPFT VTDHTPMETV
710 720 730 740
VDIFRKLGLR QCLVTRSGRL LGIITKKDVL RHMAQMANQD PESIMFN
Length:747
Mass (Da):83,733
Last modified:July 27, 2011 - v2
Checksum:iF88340263F140E30
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti284 – 2841A → R in CAA90150 (PubMed:7670496).Curated
Sequence conflicti437 – 4371I → Y in CAA90150 (PubMed:7670496).Curated
Sequence conflicti592 – 5921G → E in CAA90150 (PubMed:7670496).Curated
Sequence conflicti684 – 6841L → F in CAA90150 (PubMed:7670496).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49916 mRNA. Translation: CAA90150.1.
BC110668 mRNA. Translation: AAI10669.1.
CH466983 Genomic DNA. Translation: EDL01090.1.
CCDSiCCDS20786.1.
PIRiI48294.
RefSeqiNP_035464.3. NM_011334.4.
XP_006539564.1. XM_006539501.2.
XP_006539565.1. XM_006539502.2.
XP_011248728.1. XM_011250426.1.
UniGeneiMm.297883.
Mm.488576.

Genome annotation databases

EnsembliENSMUST00000000619; ENSMUSP00000000619; ENSMUSG00000000605.
GeneIDi12727.
KEGGimmu:12727.
UCSCiuc009fcq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49916 mRNA. Translation: CAA90150.1.
BC110668 mRNA. Translation: AAI10669.1.
CH466983 Genomic DNA. Translation: EDL01090.1.
CCDSiCCDS20786.1.
PIRiI48294.
RefSeqiNP_035464.3. NM_011334.4.
XP_006539564.1. XM_006539501.2.
XP_006539565.1. XM_006539502.2.
XP_011248728.1. XM_011250426.1.
UniGeneiMm.297883.
Mm.488576.

3D structure databases

ProteinModelPortaliQ61418.
SMRiQ61418. Positions 135-559, 579-740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000619.

PTM databases

iPTMnetiQ61418.
PhosphoSiteiQ61418.

Proteomic databases

EPDiQ61418.
MaxQBiQ61418.
PaxDbiQ61418.
PRIDEiQ61418.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000619; ENSMUSP00000000619; ENSMUSG00000000605.
GeneIDi12727.
KEGGimmu:12727.
UCSCiuc009fcq.2. mouse.

Organism-specific databases

CTDi1183.
MGIiMGI:104571. Clcn4.

Phylogenomic databases

eggNOGiKOG0475. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
HOVERGENiHBG050984.
InParanoidiQ61418.
KOiK05012.
OMAiRESIWEF.
OrthoDBiEOG7MWGXT.
TreeFamiTF313867.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

NextBioi282022.
PROiQ61418.
SOURCEiSearch...

Gene expression databases

BgeeiQ61418.
CleanExiMM_CLCN4-2.
GenevisibleiQ61418. MM.

Family and domain databases

Gene3Di1.10.3080.10. 3 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002246. Cl_channel-4.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01115. CLCHANNEL4.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Different chromosomal localization of the Clcn4 gene in Mus spretus and C57BL/6J mice."
    Rugarli E.I., Adler D.A., Borsani G., Tsuchiya K., Franco B., Hauge X., Disteche C., Chapman V., Ballabio A.
    Nat. Genet. 10:466-471(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Retina.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.

Entry informationi

Entry nameiCLCN4_MOUSE
AccessioniPrimary (citable) accession number: Q61418
Secondary accession number(s): Q2TAX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.