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Protein

H(+)/Cl(-) exchange transporter 4

Gene

Clcn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168ChlorideBy similarity1
Sitei211Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity1
Sitei268Mediates proton transfer from the protein to the inner aqueous phaseBy similarity1
Binding sitei456Chloride; via amide nitrogenBy similarity1
Binding sitei559ChlorideBy similarity1
Binding sitei597ATP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi618 – 620ATPBy similarity3
Nucleotide bindingi725 – 728ATPBy similarity4

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Chloride, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter 4
Alternative name(s):
Chloride channel protein 4
Short name:
ClC-4
Chloride transporter ClC-4
Gene namesi
Name:Clcn4
Synonyms:Clc4, Clcn4-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104571. Clcn4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54CytoplasmicBy similarityAdd BLAST54
Transmembranei55 – 92HelicalBy similarityAdd BLAST38
Transmembranei138 – 161HelicalBy similarityAdd BLAST24
Intramembranei170 – 177HelicalBy similarity8
Transmembranei187 – 205HelicalBy similarityAdd BLAST19
Transmembranei211 – 230HelicalBy similarityAdd BLAST20
Intramembranei242 – 254HelicalBy similarityAdd BLAST13
Intramembranei258 – 266HelicalBy similarity9
Transmembranei278 – 296HelicalBy similarityAdd BLAST19
Transmembranei320 – 345HelicalBy similarityAdd BLAST26
Transmembranei352 – 372HelicalBy similarityAdd BLAST21
Transmembranei429 – 449HelicalBy similarityAdd BLAST21
Transmembranei454 – 473HelicalBy similarityAdd BLAST20
Intramembranei501 – 515HelicalBy similarityAdd BLAST15
Intramembranei519 – 530HelicalBy similarityAdd BLAST12
Intramembranei531 – 534Note=Loop between two helicesBy similarity4
Transmembranei535 – 553HelicalBy similarityAdd BLAST19
Topological domaini554 – 747CytoplasmicBy similarityAdd BLAST194

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944441 – 747H(+)/Cl(-) exchange transporter 4Add BLAST747

Proteomic databases

MaxQBiQ61418.
PaxDbiQ61418.
PRIDEiQ61418.

PTM databases

iPTMnetiQ61418.
PhosphoSitePlusiQ61418.

Expressioni

Gene expression databases

BgeeiENSMUSG00000000605.
CleanExiMM_CLCN4-2.
ExpressionAtlasiQ61418. baseline and differential.
GenevisibleiQ61418. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000619.

Structurei

3D structure databases

ProteinModelPortaliQ61418.
SMRiQ61418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini587 – 653CBS 1PROSITE-ProRule annotationAdd BLAST67
Domaini680 – 742CBS 2PROSITE-ProRule annotationAdd BLAST63

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi167 – 171Selectivity filter part_1By similarity5
Motifi209 – 213Selectivity filter part_2By similarity5
Motifi454 – 458Selectivity filter part_3By similarity5

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0475. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
HOVERGENiHBG050984.
InParanoidiQ61418.
KOiK05012.
OMAiRESIWEF.
OrthoDBiEOG091G01YH.
TreeFamiTF313867.

Family and domain databases

Gene3Di1.10.3080.10. 3 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002246. Cl_channel-4.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01115. CLCHANNEL4.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFLEEPFPD VGTYEDFHTI DWLREKSRDT DRHRKITSKS KESIWEFIKS
60 70 80 90 100
LLDAWSGWVV MLLIGLLAGT LAGVIDLAVD WMTDLKEGVC LSAFWYSHEQ
110 120 130 140 150
CCWTSNETTF EDRDKCPLWQ KWSELLLSQS EGASAYILNY LMYILWALLF
160 170 180 190 200
AFLAVSLVRV FAPYACGSGI PEIKTILSGF IIRGYLGKWT LLIKTVTLVL
210 220 230 240 250
VVSSGLSLGK EGPLVHVACC CGNFFSSLFS KYSKNEGKRR EVLSAAAAAG
260 270 280 290 300
VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN
310 320 330 340 350
SRLVLFYVEY HTPWYMAELF PFILLGVFGG LWGTLFTRCN IAWCRRRKTT
360 370 380 390 400
RLGRYPVLEV IAVTAVTAIV AYPNPYTRQS TSELISELFN DCGALESSQL
410 420 430 440 450
CDYINDPNMT RPVDDIPDRP AGVGVYTAMW QLALALIFKI VITIFTFGMK
460 470 480 490 500
IPSGLFIPSM AVGAMAGRMV GIGVEQLAYH HHDWIIFRNW CRPGADCVTP
510 520 530 540 550
GLYAMVGAAA CLGGVTRMTV SLVVIMFELT GGLEYIVPLM AAAVTSKWVA
560 570 580 590 600
DAFGKEGIYE AHIHLNGYPF LDVKDEFTHR TLATDVMRPR RGEPPLSVLT
610 620 630 640 650
QDSMTVEDVE TLIKETDYNG FPVLVSRDSE RLIGFAQRRE LILAIKNARQ
660 670 680 690 700
RQEGIVSNSI MYFTEEPPEL PANSPHPLKL RRILNLSPFT VTDHTPMETV
710 720 730 740
VDIFRKLGLR QCLVTRSGRL LGIITKKDVL RHMAQMANQD PESIMFN
Length:747
Mass (Da):83,733
Last modified:July 27, 2011 - v2
Checksum:iF88340263F140E30
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti284A → R in CAA90150 (PubMed:7670496).Curated1
Sequence conflicti437I → Y in CAA90150 (PubMed:7670496).Curated1
Sequence conflicti592G → E in CAA90150 (PubMed:7670496).Curated1
Sequence conflicti684L → F in CAA90150 (PubMed:7670496).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49916 mRNA. Translation: CAA90150.1.
BC110668 mRNA. Translation: AAI10669.1.
CH466983 Genomic DNA. Translation: EDL01090.1.
CCDSiCCDS20786.1.
PIRiI48294.
RefSeqiNP_035464.3. NM_011334.4.
XP_006539564.1. XM_006539501.3.
XP_006539565.1. XM_006539502.3.
XP_011248728.1. XM_011250426.2.
UniGeneiMm.297883.
Mm.488576.

Genome annotation databases

EnsembliENSMUST00000000619; ENSMUSP00000000619; ENSMUSG00000000605.
GeneIDi12727.
KEGGimmu:12727.
UCSCiuc009fcq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49916 mRNA. Translation: CAA90150.1.
BC110668 mRNA. Translation: AAI10669.1.
CH466983 Genomic DNA. Translation: EDL01090.1.
CCDSiCCDS20786.1.
PIRiI48294.
RefSeqiNP_035464.3. NM_011334.4.
XP_006539564.1. XM_006539501.3.
XP_006539565.1. XM_006539502.3.
XP_011248728.1. XM_011250426.2.
UniGeneiMm.297883.
Mm.488576.

3D structure databases

ProteinModelPortaliQ61418.
SMRiQ61418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000619.

PTM databases

iPTMnetiQ61418.
PhosphoSitePlusiQ61418.

Proteomic databases

MaxQBiQ61418.
PaxDbiQ61418.
PRIDEiQ61418.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000619; ENSMUSP00000000619; ENSMUSG00000000605.
GeneIDi12727.
KEGGimmu:12727.
UCSCiuc009fcq.2. mouse.

Organism-specific databases

CTDi1183.
MGIiMGI:104571. Clcn4.

Phylogenomic databases

eggNOGiKOG0475. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
HOVERGENiHBG050984.
InParanoidiQ61418.
KOiK05012.
OMAiRESIWEF.
OrthoDBiEOG091G01YH.
TreeFamiTF313867.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ61418.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000605.
CleanExiMM_CLCN4-2.
ExpressionAtlasiQ61418. baseline and differential.
GenevisibleiQ61418. MM.

Family and domain databases

Gene3Di1.10.3080.10. 3 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002246. Cl_channel-4.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01115. CLCHANNEL4.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN4_MOUSE
AccessioniPrimary (citable) accession number: Q61418
Secondary accession number(s): Q2TAX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.