GTPase HRas precursor - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (?) or add your own

Q61411 (RASH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTPase HRas

Alternative name(s):
H-Ras-1
Transforming protein p21
c-H-ras
p21ras

Cleaved into the following chain:

GTPase HRas, N-terminally processed

Gene names

Name:	Hras1
Synonyms:	Hras

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Enzyme regulation	Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Subunit structure	Forms a signaling complex with RASGRP1 and DGKZ. In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C and RASSF5. Interacts with PDE6D. Interacts with IKZF3. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain). Interacts with GNB2L1 By similarity. Interacts with RGL3. Interacts with HSPD1. Ref.7 Ref.8
Subcellular location	Cell membrane By similarity. Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Note: Shuttles between the plasma membrane and the Golgi apparatus. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form By similarity.
Post-translational modification	Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi By similarity. S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.
Sequence caution	The sequence AAH61885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cell membrane Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Disease	Proto-oncogene
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Palmitate Prenylation S-nitrosylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	Ras protein signal transduction Traceable author statement. Source: MGI cell aging Inferred from direct assay. Source: MGI cell proliferation Inferred from physical interaction. Source: MGI endocytosis Inferred from direct assay. Source: MGI induction of apoptosis by intracellular signals Inferred from mutant phenotype. Source: MGI negative regulation of neuron apoptosis Inferred from direct assay. Source: MGI positive regulation of cell proliferation Inferred from direct assay. Source: MGI positive regulation of gene expression Inferred from direct assay. Source: MGI regulation of long-term neuronal synaptic plasticity Inferred from mutant phenotype. Source: MGI
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	GTP binding Inferred from direct assay. Source: MGI GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rassf5	Q5EBH1	2	EBI-400273,EBI-960530

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q61411-1)

Also known as: p21; H-Ras4A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q61411-2)

Also known as: p19; H-RasIDX;

The sequence of this isoform differs from the canonical sequence as follows:
152-189: VEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS → SRSGSSSGTLWDPPSPGTHVTQRPSSWRGGCLLYTSP

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sequence conflict

163

D → G in CAD24593. Ref.6

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 186

186

GTPase HRas

PRO_0000042998

Initiator methionine

Removed; alternate By similarity

Chain

2 – 186

185

GTPase HRas, N-terminally processed

PRO_0000326478

Propeptide

187 – 189

Removed in mature form By similarity

PRO_0000042999

Regions

Nucleotide binding

10 – 17

GTP By similarity

Nucleotide binding

57 – 61

GTP By similarity

Nucleotide binding

116 – 119

GTP By similarity

Region

166 – 185

Hypervariable region

Motif

32 – 40

Effector region

Amino acid modifications

Modified residue

N-acetylmethionine; in GTPase HRas; alternate By similarity

Modified residue

N-acetylthreonine; in GTPase HRas, N-terminally processed By similarity

Modified residue

118

S-nitrosocysteine By similarity

Modified residue

186

Cysteine methyl ester By similarity

Lipidation

181

S-palmitoyl cysteine By similarity

Lipidation

184

S-(15-deoxy-Delta12,14-prostaglandin J2-9-yl)cysteine; alternate By similarity

Lipidation

184

S-palmitoyl cysteine; alternate By similarity

Lipidation

186

S-farnesyl cysteine By similarity

Natural variations

Alternative sequence

152 – 189

VEDAF…KCVLS → SRSGSSSGTLWDPPSPGTHV TQRPSSWRGGCLLYTSP in isoform 2.

VSP_041598

Experimental info

Mutagenesis

Q → R: Found in chemically induced liver tumors. Ref.4

Sequence conflict

I → Y in CAA90306. Ref.1

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (p21) (H-Ras4A) [UniParc]. Last modified July 27, 2011. Version 2. Checksum: EE6DC2D933E2856A Show »	FASTA	189	21,298
10 20 30 40 50 60 MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG 70 80 90 100 110 120 QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL 130 140 150 160 170 180 AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS « Hide
Isoform 2 (p19) (H-RasIDX) [UniParc]. Checksum: BD42AE50A77341FF Show »	FASTA	188	20,880
10 20 30 40 50 60 MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG 70 80 90 100 110 120 QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL 130 140 150 160 170 180 AARTVESRQA QDLARSYGIP YIETSAKTRQ GSRSGSSSGT LWDPPSPGTH VTQRPSSWRG GCLLYTSP « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of c-H-ras-1 gene from B6C3F1 mice." Przybojewska B., Plucienniczak G. Acta Biochim. Pol. 43:575-578(1996) [PubMed: 8922043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X C3H. Tissue: Liver.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Olfactory epithelium.
[4]	"Spontaneous mutation at codon 61 of the Ha-ras gene in the nascent liver of B6C3F1, C3H/He and C57BL/6 mice." Moulds B.A., Goodman J.I. Mutat. Res. 311:1-7(1994) [PubMed: 7526162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-75, MUTAGENESIS OF GLN-61.
[5]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 136-147, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[6]	"Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus." Guil S., de La Iglesia N., Fernandez-Larrea J., Cifuentes D., Ferrer J.C., Guinovart J.J., Bach-Elias M. Cancer Res. 63:5178-5187(2003) [PubMed: 14500341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 151-189 (ISOFORM 2), ALTERNATIVE SPLICING. Strain: NIH/3T3.
[7]	"An interaction between p21ras and heat shock protein hsp60, a chaperonin." Ikawa S., Weinberg R.A. Proc. Natl. Acad. Sci. U.S.A. 89:2012-2016(1992) [PubMed: 1347942] [Abstract] Cited for: INTERACTION WITH HSPD1.
[8]	"A novel RalGEF-like protein, RGL3, as a candidate effector for rit and Ras." Shao H., Andres D.A. J. Biol. Chem. 275:26914-26924(2000) [PubMed: 10869344] [Abstract] Cited for: INTERACTION WITH RGL3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50013 Genomic DNA. Translation: CAA90306.1. AC108908 Genomic DNA. No translation available. BC061885 mRNA. Translation: AAH61885.1. Different initiation. S74119 Genomic DNA. Translation: AAP21090.1. AJ437023 mRNA. Translation: CAD24593.1.
IPI	IPI00605942. IPI01023179.
PIR	S57718.
RefSeq	NP_001123915.1. NM_001130443.1. NP_001123916.1. NM_001130444.1. NP_032310.2. NM_008284.2.
UniGene	Mm.334313.
3D structure databases
ProteinModelPortal	Q61411.
SMR	Q61411. Positions 1-166.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29361N.
IntAct	Q61411. 8 interactions.
MINT	MINT-1215825.
STRING	Q61411.
PTM databases
PhosphoSite	Q61411.
Proteomic databases
PRIDE	Q61411.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026572; ENSMUSP00000026572; ENSMUSG00000025499. ENSMUST00000097957; ENSMUSP00000095570; ENSMUSG00000025499. ENSMUST00000168550; ENSMUSP00000132110; ENSMUSG00000025499.
GeneID	15461.
KEGG	mmu:15461.
Organism-specific databases
CTD	15461.
MGI	MGI:96224. Hras1.
Phylogenomic databases
HOVERGEN	HBG009351.
OrthoDB	EOG4BRWMX.
PhylomeDB	Q61411.
Gene expression databases
ArrayExpress	Q61411.
Bgee	Q61411.
CleanEx	MM_HRAS1.
Genevestigator	Q61411.
GermOnline	ENSMUSG00000025499. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
KO	K02833.
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	288278.
SOURCE	Search...

Entry information

Entry name

RASH_MOUSE

Accession

Primary (citable) accession number: Q61411
Secondary accession number(s): Q6P716, Q80WD2, Q811B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents