Reviewed, UniProtKB/Swiss-Prot Q61411 (RASH_MOUSE)

Names and origin

Protein names

Recommended name:
    GTPase HRas
Alternative name(s):
    Transforming protein p21
    p21ras
    H-Ras-1
    c-H-ras
Cleaved into the following chain:
    1- Recommended name:
            GTPase HRas, N-terminally processed

Gene names

Name:	Hras1
Synonyms:	Hras

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Enzyme regulation	Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Subunit structure	Forms a signaling complex with RASGRP1 and DGKZ. In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C and RASSF5 By similarity. Interacts with RGL3.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Note: Shuttles between the plasma membrane and the Golgi apparatus By similarity.
Post-translational modification	Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi By similarity. S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Golgi apparatus Membrane
Disease	Proto-oncogene
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Palmitate Prenylation S-nitrosylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	Ras protein signal transduction Traceable author statement. Source: MGI cell aging Inferred from direct assay. Source: MGI cell proliferation Inferred from physical interaction. Source: MGI endocytosis Inferred from direct assay. Source: MGI negative regulation of neuron apoptosis Inferred from direct assay. Source: MGI positive regulation of cell proliferation Inferred from direct assay. Source: MGI regulation of long-term neuronal synaptic plasticity Inferred from mutant phenotype. Source: MGI
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pik3r1	P26450	1	EBI-400273,EBI-641764
Rassf5	Q5EBH1	2	EBI-400273,EBI-960530
Rassf5	Q5EBH1-1	2	EBI-400273,EBI-960543

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	GTPase HRas		PRO_0000042998
	Initiator methionine	1	1	Removed; alternate By similarity
	Chain	2 – 186	185	GTPase HRas, N-terminally processed		PRO_0000326478
	Propeptide	187 – 189	3	Removed in mature form By similarity		PRO_0000042999
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	116 – 119	4	GTP By similarity
	Region	166 – 185	20	Hypervariable region
	Motif	32 – 40	9	Effector region
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine; in GTPase HRas; alternate By similarity
	Modified residue	2	1	N-acetylthreonine; in GTPase HRas, N-terminally processed By similarity
	Modified residue	118	1	S-nitrosocysteine By similarity
	Modified residue	186	1	Cysteine methyl ester By similarity
	Lipidation	181	1	S-palmitoyl cysteine By similarity
	Lipidation	184	1	S-palmitoyl cysteine By similarity
	Lipidation	186	1	S-farnesyl cysteine By similarity
Experimental info
	Mutagenesis	61	1	Q → R: Found in chemically induced liver tumors. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61411-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: FA3DC2D927B28A3A	FASTA	189	21,348
	10 20 30 40 50 60 MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDYLDTAG 70 80 90 100 110 120 QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL 130 140 150 160 170 180 AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS

References

[1]	"Nucleotide sequence of c-H-ras-1 gene from B6C3F1 mice." Przybojewska B., Plucienniczak G. Acta Biochim. Pol. 43:575-578(1996) [PubMed: 8922043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X C3H. Tissue: Liver.
[2]	"Spontaneous mutation at codon 61 of the Ha-ras gene in the nascent liver of B6C3F1, C3H/He and C57BL/6 mice." Moulds B.A., Goodman J.I. Mutat. Res. 311:1-7(1994) [PubMed: 7526162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-75, MUTAGENESIS OF GLN-61.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 136-147, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"A novel RalGEF-like protein, RGL3, as a candidate effector for rit and Ras." Shao H., Andres D.A. J. Biol. Chem. 275:26914-26924(2000) [PubMed: 10869344] [Abstract] Cited for: INTERACTION WITH RGL3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z50013 Genomic DNA. Translation: CAA90306.1. S74119 Genomic DNA. Translation: AAP21090.1.
IPI	IPI00121071.
PIR	S57718.
UniGene	Mm.334313
3D structure databases
SMR	Q61411. Positions 1-166.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61411. 8 interactions.
STRING	Q61411.
PTM databases
PhosphoSite	Q61411.
Proteomic databases
PRIDE	Q61411.
Genome annotation databases
Ensembl	ENSMUST00000026572; ENSMUSP00000026572; ENSMUSG00000025499; Mus musculus. [Genome view] ENSMUST00000097957; ENSMUSP00000095570; ENSMUSG00000025499; Mus musculus. [Genome view]
UCSC	uc009kjw.1. mouse.
Organism-specific databases
MGI	MGI:96224. Hras1.
Phylogenomic databases
HOVERGEN	Q61411.
Gene expression databases
ArrayExpress	Q61411.
Bgee	Q61411.
CleanEx	MM_HRAS1.
Genevestigator	Q61411.
GermOnline	ENSMUSG00000025499. Mus musculus.
Family and domain databases
InterPro	IPR003577. GTPase_Ras. IPR013753. Ras. IPR001806. Ras_GTPase. IPR015592. Ras_Ras_related. IPR020849. Ras_small_GTPase. IPR005225. Small_GTP_bd. [Graphical view]
PANTHER	PTHR11708:SF125. Ras_Ras_related. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288278.
SOURCE	Search...

Entry information

Entry name

RASH_MOUSE

Accession

Primary (citable) accession number: Q61411
Secondary accession number(s): Q80WD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)