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Protein

cGMP-dependent protein kinase 2

Gene

Prkg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Crucial regulator of intestinal secretion and bone growth (PubMed:8953039). Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (By similarity). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (By similarity). Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 AND MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (PubMed:19282289).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Binding of cGMP results in enzyme activation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei347cAMP or cGMP 2By similarity1
Binding sitei412cAMP or cGMP 2By similarity1
Binding sitei415cAMP or cGMP 2By similarity1
Binding sitei482ATPPROSITE-ProRule annotation1
Active sitei576Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi232 – 235cAMP or cGMP 1By similarity4
Nucleotide bindingi242 – 243cAMP or cGMP 1By similarity2
Nucleotide bindingi357 – 359cAMP or cGMP 2By similarity3
Nucleotide bindingi366 – 367cAMP or cGMP 2By similarity2
Nucleotide bindingi459 – 467ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cellular hypotonic response Source: CAFA
  • circadian regulation of gene expression Source: MGI
  • circadian rhythm Source: MGI
  • negative regulation of cell volume Source: CAFA
  • protein phosphorylation Source: MGI

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.12. 3474.
ReactomeiR-MMU-4086398. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent protein kinase 2 (EC:2.7.11.12)
Short name:
cGK 2
Short name:
cGK2
Alternative name(s):
cGMP-dependent protein kinase II
Short name:
cGKII
Gene namesi
Name:Prkg2
Synonyms:Prkgr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:108173. Prkg2.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • plasma membrane Source: MGI

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Dwarfism, caused by a severe defect in endochondral ossification at the growth plates.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000861242 – 762cGMP-dependent protein kinase 2Add BLAST761

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei110PhosphoserineBy similarity1
Modified residuei117PhosphoserineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei609PhosphothreonineCombined sources1

Post-translational modificationi

Myristoylation mediates membrane localization.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiQ61410.
PaxDbiQ61410.
PRIDEiQ61410.

PTM databases

iPTMnetiQ61410.
PhosphoSitePlusiQ61410.

Expressioni

Gene expression databases

CleanExiMM_PRKG2.

Interactioni

Subunit structurei

Interacts with GRIA1/GLUR1.By similarity

Protein-protein interaction databases

IntActiQ61410. 1 interactor.
STRINGi10090.ENSMUSP00000124963.

Structurei

3D structure databases

ProteinModelPortaliQ61410.
SMRiQ61410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini453 – 711Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini712 – 762AGC-kinase C-terminalAdd BLAST51

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 283cGMP-binding, high affinity; cAMP-binding, moderate affinityBy similarityAdd BLAST116
Regioni286 – 416cGMP-binding, high affinity; cAMP-binding, low affinityBy similarityAdd BLAST131

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0616. Eukaryota.
ENOG410XPQQ. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG006211.
InParanoidiQ61410.

Family and domain databases

CDDicd05572. STKc_cGK. 1 hit.
Gene3Di2.60.120.10. 2 hits.
InterProiView protein in InterPro
IPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR008271. Ser/Thr_kinase_AS.
IPR035014. STKc_cGK.
PfamiView protein in Pfam
PF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
PIRSFiPIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTSiPR00104. CGMPKINASE.
SMARTiView protein in SMART
SM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61410-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNGSVKPKH AKHPDGHSGN LSNEALRSKV LELERELRRK DAELQEREYH
60 70 80 90 100
LKELREQLAK QTVAIAELTE ELQSKCIQLN KLQDVIHVQG GSPLQASPDK
110 120 130 140 150
VPLDVHRKTS GLVSLHSRRG AKAGVSAEPT TRTYDLNKPP EFSFEKARVR
160 170 180 190 200
KDSSEKKLIT DALNKNQFLK RLDPQQIKDM VECMYGEKLS TGSYVIKQGE
210 220 230 240 250
PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC TRTASVKAIT
260 270 280 290 300
NVKTWALDRE VFQNIMRRTA QARDEEYRNF LRSVSLLKNL PEDKLTKIID
310 320 330 340 350
CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ
360 370 380 390 400
KGEYFGEKAL ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFDELQKY
410 420 430 440 450
LEGYVATLNR DDEKRHAKRS MSSWKLSKAL SLEMIQLKEK VARFSSTSPF
460 470 480 490 500
QNLEIIATLG VGGFGRVELV KVKNENVAFA MKCIRKKHIV DTKQQEHVYS
510 520 530 540 550
EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS ILRDRGSFDE
560 570 580 590 600
PTSKFCVACV TEAFDYLHLL GIIYRDLKPE NLILDADGYL KLVDFGFAKK
610 620 630 640 650
IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF
660 670 680 690 700
SGIDQMMTYN LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN
710 720 730 740 750
GINDIKKHRW LNGFNWEGLK ARSLPSPLRR ELSGPIDHSY FDKYPPEKGV
760
PPDEMSGWDK DF
Length:762
Mass (Da):87,085
Last modified:November 1, 1996 - v1
Checksum:i74F9D9A6835711A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12460 mRNA. Translation: AAA02572.1.
CCDSiCCDS19459.1.
PIRiA46590.
UniGeneiMm.263002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12460 mRNA. Translation: AAA02572.1.
CCDSiCCDS19459.1.
PIRiA46590.
UniGeneiMm.263002.

3D structure databases

ProteinModelPortaliQ61410.
SMRiQ61410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61410. 1 interactor.
STRINGi10090.ENSMUSP00000124963.

PTM databases

iPTMnetiQ61410.
PhosphoSitePlusiQ61410.

Proteomic databases

MaxQBiQ61410.
PaxDbiQ61410.
PRIDEiQ61410.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:108173. Prkg2.

Phylogenomic databases

eggNOGiKOG0616. Eukaryota.
ENOG410XPQQ. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG006211.
InParanoidiQ61410.

Enzyme and pathway databases

BRENDAi2.7.11.12. 3474.
ReactomeiR-MMU-4086398. Ca2+ pathway.

Miscellaneous databases

PROiPR:Q61410.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PRKG2.

Family and domain databases

CDDicd05572. STKc_cGK. 1 hit.
Gene3Di2.60.120.10. 2 hits.
InterProiView protein in InterPro
IPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR008271. Ser/Thr_kinase_AS.
IPR035014. STKc_cGK.
PfamiView protein in Pfam
PF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
PIRSFiPIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTSiPR00104. CGMPKINASE.
SMARTiView protein in SMART
SM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKGP2_MOUSE
AccessioniPrimary (citable) accession number: Q61410
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.