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Q61409

- PDE3B_MOUSE

UniProt

Q61409 - PDE3B_MOUSE

Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Gene

Pde3b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis By similarity.By similarity

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by cGMP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei713 – 7131Proton donorBy similarity
    Metal bindingi717 – 7171Divalent metal cation 1By similarity
    Metal bindingi797 – 7971Divalent metal cation 1By similarity
    Metal bindingi798 – 7981Divalent metal cation 1By similarity
    Metal bindingi798 – 7981Divalent metal cation 2By similarity
    Metal bindingi913 – 9131Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. protein kinase B binding Source: BHF-UCL

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. cellular response to insulin stimulus Source: BHF-UCL
    3. endocrine pancreas development Source: MGI
    4. glucose homeostasis Source: MGI
    5. negative regulation of angiogenesis Source: UniProtKB
    6. regulation of insulin secretion Source: MGI
    7. signal transduction Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Angiogenesis

    Keywords - Ligandi

    cAMP, cGMP, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC:3.1.4.17)
    Alternative name(s):
    CGIPDE1
    Cyclic GMP-inhibited phosphodiesterase B
    Short name:
    CGI-PDE B
    Gene namesi
    Name:Pde3b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1333863. Pde3b.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endoplasmic reticulum Source: BHF-UCL
    3. Golgi apparatus Source: BHF-UCL
    4. integral component of membrane Source: UniProtKB-KW
    5. membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11001100cGMP-inhibited 3',5'-cyclic phosphodiesterase BPRO_0000198803Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei273 – 2731Phosphoserine; by PKB/AKT1 or PKB/AKT21 Publication
    Modified residuei421 – 4211PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ61409.
    PRIDEiQ61409.

    Expressioni

    Tissue specificityi

    Abundant in adipose tissues.

    Gene expression databases

    CleanExiMM_PDE3B.
    GenevestigatoriQ61409.

    Interactioni

    Subunit structurei

    Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6 By similarity.By similarity

    Protein-protein interaction databases

    MINTiMINT-4997265.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61409.
    SMRiQ61409. Positions 632-1055.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei140 – 16021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei198 – 21821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei225 – 24521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2828Interaction with RAPGEF3By similarityAdd
    BLAST
    Regioni415 – 43925Interaction with PIK3R6By similarityAdd
    BLAST
    Regioni689 – 1054366CatalyticBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1044 – 107936Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145074.
    HOGENOMiHOG000060144.
    HOVERGENiHBG053541.
    InParanoidiQ61409.
    KOiK13296.
    PhylomeDBiQ61409.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61409-1 [UniParc]FASTAAdd to Basket

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    MRKDERERDA PAMRSPPPPP ASAASPPESL RNGYVKSCVS PLRQDPPRSF     50
    FFHLCRFCNV EPPAASLRAG ARLSLGVLAA FVLAALLGAR PERWAAAAAG 100
    LRTLLSACSL SLSPLFSIAC AFFFLTCFLT RAQRGPGRGA GSWWLLALPA 150
    CCYLGDFAAW QWWSWLRGEP AAAGRLCLVL SCVGLLTLAP RVRLRHGVLV 200
    LLFAGLVWWV SFSGLGALPP ALRPLLSCLV GGAGCLLALG LDHFFHVRGA 250
    SPPPRSASTA EEKVPVIRPR RRSSCVSLGE SAAGYYGSGK MFRRPSLPCI 300
    SREQMILWDW DLKQWCKPHY QNSGGGNGVD LSVLNEARNM VSDLLIDPSL 350
    PPQVISSLRS ISSLMGAFSG SCRPKINSFT PFPGFYPCSE VEDPVEKGDR 400
    KLHKGLSGRT SFPTPQLRRS SGASSLLTNE HCSRWDRSSG KRSYQELSVS 450
    SHGCHLNGPF SSNLFTIPKQ RSSSVSLTHH AGLRRAGALP SHSLLNSSSH 500
    VPVSAGSLTN RSPIGFPDTT DFLTKPNIIL HRSLGSVSSA ADFHQYLRNS 550
    DSNLCSSCGH QILKYVSTCE PDGTDHPSEK SGEEDSSVFS KEPLNIVETQ 600
    EEETMKKACR ELFLEGDSHL MEEAQQPNID QEVSLDPMLV EDYDSLIEKM 650
    NNWNFQIFEL VEKMGEKSGR ILSQVMYTLF QDTGLLETFK IPTQEFMNYF 700
    RALENGYRDI PYHNRVHATD VLHAVWYLTT RPIPGLPQIH NNHETETKAD 750
    SDGRLGSGQI AYISSKSCCI PDMSYGCLSS NIPALELMAL YVAAAMHDYD 800
    HPGRTNAFLV ATNAPQAVLY NDRSVLENHH AASAWNLYLS RPEYNFLLNL 850
    DHMEFKRFRF LVIEAILATD LKKHFDFLAE FNAKANDVNS NGIEWSSEND 900
    RLLVCQVCIK LADINGPAKD RDLHLRWTEG IVNEFYEQGD EEAALGLPIS 950
    PFMDRSSPQL AKLQESFITH IVGPLCNSYD AAGLLPGQWI ETEEGDDTES 1000
    DDDDDDDDGD GGEELDSDDE ETEDNLNPKP QRRKGRRRIF CQLMHHLTEN 1050
    HKIWKEIIEE EEEKCKAEGN KLQVDNASLP QADEIQVIEE ADEEEEQMFE 1100
    Length:1,100
    Mass (Da):122,154
    Last modified:January 9, 2007 - v2
    Checksum:i92E36B28D4A5FDF1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101A → T in CAA10639. (PubMed:10454575)Curated
    Sequence conflicti261 – 2611E → D in CAA10639. (PubMed:10454575)Curated
    Sequence conflicti288 – 2903SGK → IPE in CAA64775. (PubMed:8921398)Curated
    Sequence conflicti439 – 4391S → G in CAA10639. (PubMed:10454575)Curated
    Sequence conflicti444 – 4441Y → H in CAA10639. (PubMed:10454575)Curated
    Sequence conflicti465 – 4651F → M in CAA10639. (PubMed:10454575)Curated
    Sequence conflicti756 – 7561G → S in CAA10639. (PubMed:10454575)Curated
    Sequence conflicti944 – 9441A → T in CAA10639. (PubMed:10454575)Curated
    Sequence conflicti944 – 9441A → T in CAA64775. (PubMed:8921398)Curated
    Sequence conflicti1082 – 10821A → T in CAA64775. (PubMed:8921398)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132271 mRNA. Translation: CAA10639.1.
    AF547435 mRNA. Translation: AAN52086.1.
    X95521 mRNA. Translation: CAA64775.1.
    RefSeqiNP_035185.2. NM_011055.2.
    UniGeneiMm.430730.

    Genome annotation databases

    GeneIDi18576.
    KEGGimmu:18576.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132271 mRNA. Translation: CAA10639.1 .
    AF547435 mRNA. Translation: AAN52086.1 .
    X95521 mRNA. Translation: CAA64775.1 .
    RefSeqi NP_035185.2. NM_011055.2.
    UniGenei Mm.430730.

    3D structure databases

    ProteinModelPortali Q61409.
    SMRi Q61409. Positions 632-1055.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4997265.

    Proteomic databases

    PaxDbi Q61409.
    PRIDEi Q61409.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 18576.
    KEGGi mmu:18576.

    Organism-specific databases

    CTDi 5140.
    MGIi MGI:1333863. Pde3b.

    Phylogenomic databases

    eggNOGi NOG145074.
    HOGENOMi HOG000060144.
    HOVERGENi HBG053541.
    InParanoidi Q61409.
    KOi K13296.
    PhylomeDBi Q61409.

    Miscellaneous databases

    ChiTaRSi PDE3B. mouse.
    NextBioi 294434.
    PROi Q61409.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_PDE3B.
    Genevestigatori Q61409.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insulin-induced phosphorylation and activation of cyclic nucleotide phosphodiesterase 3B by the serine-threonine kinase Akt."
      Kitamura T., Kitamura Y., Kuroda S., Hino Y., Ando M., Kotani K., Konishi H., Matsuzaki H., Kikkawa U., Ogawa W., Kasuga M.
      Mol. Cell. Biol. 19:6286-6296(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-273.
    2. "Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide phosphodiesterase 3B (Pde3b) gene in differentiating 3T3-L1 preadipocytes."
      Liu H., Tang J.R., Choi Y.H., Napolitano M., Hockman S., Taira M., Degerman E., Manganiello V.C.
      J. Biol. Chem. 281:21096-21113(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: J125.
    3. "Molecular cloning and chromosomal assignment of the human homologue of the rat cGMP-inhibited phosphodiesterase 1 (PDE3A) -- a gene involved in fat metabolism located at 11p15.1."
      Loebbert R.W., Winterpacht A., Seipel B., Zabel B.U.
      Genomics 37:211-218(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 287-1085.
      Strain: Swiss.
      Tissue: Adipose tissue.
    4. "PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects."
      Patrucco E., Notte A., Barberis L., Selvetella G., Maffei A., Brancaccio M., Marengo S., Russo G., Azzolino O., Rybalkin S.D., Silengo L., Altruda F., Wetzker R., Wymann M.P., Lembo G., Hirsch E.
      Cell 118:375-387(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIK3CG.

    Entry informationi

    Entry nameiPDE3B_MOUSE
    AccessioniPrimary (citable) accession number: Q61409
    Secondary accession number(s): Q8CIX5, Q9Z1J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3