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Q61409

- PDE3B_MOUSE

UniProt

Q61409 - PDE3B_MOUSE

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Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Gene

Pde3b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by cGMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei713 – 7131Proton donorBy similarity
Metal bindingi717 – 7171Divalent metal cation 1By similarity
Metal bindingi797 – 7971Divalent metal cation 1By similarity
Metal bindingi798 – 7981Divalent metal cation 1By similarity
Metal bindingi798 – 7981Divalent metal cation 2By similarity
Metal bindingi913 – 9131Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. protein kinase B binding Source: BHF-UCL

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. cellular response to insulin stimulus Source: BHF-UCL
  3. endocrine pancreas development Source: MGI
  4. glucose homeostasis Source: MGI
  5. negative regulation of angiogenesis Source: UniProtKB
  6. regulation of insulin secretion Source: MGI
  7. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Angiogenesis

Keywords - Ligandi

cAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_239644. PDE3B signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC:3.1.4.17)
Alternative name(s):
CGIPDE1
Cyclic GMP-inhibited phosphodiesterase B
Short name:
CGI-PDE B
Gene namesi
Name:Pde3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1333863. Pde3b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei69 – 8921HelicalSequence AnalysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence AnalysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Transmembranei198 – 21821HelicalSequence AnalysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endoplasmic reticulum Source: BHF-UCL
  3. Golgi apparatus Source: BHF-UCL
  4. integral component of membrane Source: UniProtKB-KW
  5. membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11001100cGMP-inhibited 3',5'-cyclic phosphodiesterase BPRO_0000198803Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei273 – 2731Phosphoserine; by PKB/AKT1 or PKB/AKT21 Publication
Modified residuei421 – 4211PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ61409.
PRIDEiQ61409.

Expressioni

Tissue specificityi

Abundant in adipose tissues.

Gene expression databases

CleanExiMM_PDE3B.
GenevestigatoriQ61409.

Interactioni

Subunit structurei

Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4997265.

Structurei

3D structure databases

ProteinModelPortaliQ61409.
SMRiQ61409. Positions 632-1055.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2828Interaction with RAPGEF3By similarityAdd
BLAST
Regioni415 – 43925Interaction with PIK3R6By similarityAdd
BLAST
Regioni689 – 1054366CatalyticBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1044 – 107936Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145074.
HOGENOMiHOG000060144.
HOVERGENiHBG053541.
InParanoidiQ61409.
KOiK13296.
PhylomeDBiQ61409.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61409-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKDERERDA PAMRSPPPPP ASAASPPESL RNGYVKSCVS PLRQDPPRSF
60 70 80 90 100
FFHLCRFCNV EPPAASLRAG ARLSLGVLAA FVLAALLGAR PERWAAAAAG
110 120 130 140 150
LRTLLSACSL SLSPLFSIAC AFFFLTCFLT RAQRGPGRGA GSWWLLALPA
160 170 180 190 200
CCYLGDFAAW QWWSWLRGEP AAAGRLCLVL SCVGLLTLAP RVRLRHGVLV
210 220 230 240 250
LLFAGLVWWV SFSGLGALPP ALRPLLSCLV GGAGCLLALG LDHFFHVRGA
260 270 280 290 300
SPPPRSASTA EEKVPVIRPR RRSSCVSLGE SAAGYYGSGK MFRRPSLPCI
310 320 330 340 350
SREQMILWDW DLKQWCKPHY QNSGGGNGVD LSVLNEARNM VSDLLIDPSL
360 370 380 390 400
PPQVISSLRS ISSLMGAFSG SCRPKINSFT PFPGFYPCSE VEDPVEKGDR
410 420 430 440 450
KLHKGLSGRT SFPTPQLRRS SGASSLLTNE HCSRWDRSSG KRSYQELSVS
460 470 480 490 500
SHGCHLNGPF SSNLFTIPKQ RSSSVSLTHH AGLRRAGALP SHSLLNSSSH
510 520 530 540 550
VPVSAGSLTN RSPIGFPDTT DFLTKPNIIL HRSLGSVSSA ADFHQYLRNS
560 570 580 590 600
DSNLCSSCGH QILKYVSTCE PDGTDHPSEK SGEEDSSVFS KEPLNIVETQ
610 620 630 640 650
EEETMKKACR ELFLEGDSHL MEEAQQPNID QEVSLDPMLV EDYDSLIEKM
660 670 680 690 700
NNWNFQIFEL VEKMGEKSGR ILSQVMYTLF QDTGLLETFK IPTQEFMNYF
710 720 730 740 750
RALENGYRDI PYHNRVHATD VLHAVWYLTT RPIPGLPQIH NNHETETKAD
760 770 780 790 800
SDGRLGSGQI AYISSKSCCI PDMSYGCLSS NIPALELMAL YVAAAMHDYD
810 820 830 840 850
HPGRTNAFLV ATNAPQAVLY NDRSVLENHH AASAWNLYLS RPEYNFLLNL
860 870 880 890 900
DHMEFKRFRF LVIEAILATD LKKHFDFLAE FNAKANDVNS NGIEWSSEND
910 920 930 940 950
RLLVCQVCIK LADINGPAKD RDLHLRWTEG IVNEFYEQGD EEAALGLPIS
960 970 980 990 1000
PFMDRSSPQL AKLQESFITH IVGPLCNSYD AAGLLPGQWI ETEEGDDTES
1010 1020 1030 1040 1050
DDDDDDDDGD GGEELDSDDE ETEDNLNPKP QRRKGRRRIF CQLMHHLTEN
1060 1070 1080 1090 1100
HKIWKEIIEE EEEKCKAEGN KLQVDNASLP QADEIQVIEE ADEEEEQMFE
Length:1,100
Mass (Da):122,154
Last modified:January 9, 2007 - v2
Checksum:i92E36B28D4A5FDF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101A → T in CAA10639. (PubMed:10454575)Curated
Sequence conflicti261 – 2611E → D in CAA10639. (PubMed:10454575)Curated
Sequence conflicti288 – 2903SGK → IPE in CAA64775. (PubMed:8921398)Curated
Sequence conflicti439 – 4391S → G in CAA10639. (PubMed:10454575)Curated
Sequence conflicti444 – 4441Y → H in CAA10639. (PubMed:10454575)Curated
Sequence conflicti465 – 4651F → M in CAA10639. (PubMed:10454575)Curated
Sequence conflicti756 – 7561G → S in CAA10639. (PubMed:10454575)Curated
Sequence conflicti944 – 9441A → T in CAA10639. (PubMed:10454575)Curated
Sequence conflicti944 – 9441A → T in CAA64775. (PubMed:8921398)Curated
Sequence conflicti1082 – 10821A → T in CAA64775. (PubMed:8921398)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132271 mRNA. Translation: CAA10639.1.
AF547435 mRNA. Translation: AAN52086.1.
X95521 mRNA. Translation: CAA64775.1.
RefSeqiNP_035185.2. NM_011055.2.
UniGeneiMm.430730.

Genome annotation databases

GeneIDi18576.
KEGGimmu:18576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132271 mRNA. Translation: CAA10639.1 .
AF547435 mRNA. Translation: AAN52086.1 .
X95521 mRNA. Translation: CAA64775.1 .
RefSeqi NP_035185.2. NM_011055.2.
UniGenei Mm.430730.

3D structure databases

ProteinModelPortali Q61409.
SMRi Q61409. Positions 632-1055.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4997265.

Proteomic databases

PaxDbi Q61409.
PRIDEi Q61409.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 18576.
KEGGi mmu:18576.

Organism-specific databases

CTDi 5140.
MGIi MGI:1333863. Pde3b.

Phylogenomic databases

eggNOGi NOG145074.
HOGENOMi HOG000060144.
HOVERGENi HBG053541.
InParanoidi Q61409.
KOi K13296.
PhylomeDBi Q61409.

Enzyme and pathway databases

Reactomei REACT_239644. PDE3B signalling.

Miscellaneous databases

ChiTaRSi Pde3b. mouse.
NextBioi 294434.
PROi Q61409.
SOURCEi Search...

Gene expression databases

CleanExi MM_PDE3B.
Genevestigatori Q61409.

Family and domain databases

Gene3Di 1.10.1300.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Insulin-induced phosphorylation and activation of cyclic nucleotide phosphodiesterase 3B by the serine-threonine kinase Akt."
    Kitamura T., Kitamura Y., Kuroda S., Hino Y., Ando M., Kotani K., Konishi H., Matsuzaki H., Kikkawa U., Ogawa W., Kasuga M.
    Mol. Cell. Biol. 19:6286-6296(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-273.
  2. "Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide phosphodiesterase 3B (Pde3b) gene in differentiating 3T3-L1 preadipocytes."
    Liu H., Tang J.R., Choi Y.H., Napolitano M., Hockman S., Taira M., Degerman E., Manganiello V.C.
    J. Biol. Chem. 281:21096-21113(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: J125.
  3. "Molecular cloning and chromosomal assignment of the human homologue of the rat cGMP-inhibited phosphodiesterase 1 (PDE3A) -- a gene involved in fat metabolism located at 11p15.1."
    Loebbert R.W., Winterpacht A., Seipel B., Zabel B.U.
    Genomics 37:211-218(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 287-1085.
    Strain: Swiss.
    Tissue: Adipose tissue.
  4. "PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects."
    Patrucco E., Notte A., Barberis L., Selvetella G., Maffei A., Brancaccio M., Marengo S., Russo G., Azzolino O., Rybalkin S.D., Silengo L., Altruda F., Wetzker R., Wymann M.P., Lembo G., Hirsch E.
    Cell 118:375-387(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIK3CG.

Entry informationi

Entry nameiPDE3B_MOUSE
AccessioniPrimary (citable) accession number: Q61409
Secondary accession number(s): Q8CIX5, Q9Z1J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 9, 2007
Last modified: November 26, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3