cGMP-inhibited 3',5'-cyclic phosphodiesterase B

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Q61409 (PDE3B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
EC=3.1.4.17

Alternative name(s):
CGIPDE1
Cyclic GMP-inhibited phosphodiesterase B
Short name=CGI-PDE B

Gene names

Name:

Pde3b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1100 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism.
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Inhibited by cGMP.
Subunit structure	Interacts with PIK3CG. Ref.5
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Abundant in adipose tissues.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

Ontologies

Keywords
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Ligand	Metal-binding cAMP cGMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to insulin stimulus Inferred from expression pattern Ref.1. Source: BHF-UCL endocrine pancreas development Inferred from direct assay. Source: MGI glucose homeostasis Inferred from direct assay. Source: MGI regulation of insulin secretion Inferred from direct assay. Source: MGI signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from direct assay. Source: BHF-UCL cytosol Traceable author statement. Source: Reactome endoplasmic reticulum Inferred from direct assay. Source: BHF-UCL integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: BHF-UCL
Molecular function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase B binding Inferred from physical interaction Ref.1. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1100

1100

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

PRO_0000198803

Regions

Transmembrane

69 – 89

Helical; Potential

Transmembrane

110 – 130

Helical; Potential

Transmembrane

140 – 160

Helical; Potential

Transmembrane

170 – 190

Helical; Potential

Transmembrane

198 – 218

Helical; Potential

Transmembrane

225 – 245

Helical; Potential

Region

689 – 1054

366

Catalytic By similarity

Coiled coil

1044 – 1079

Potential

Sites

Active site

713

Proton donor By similarity

Metal binding

717

Divalent metal cation 1 By similarity

Metal binding

797

Divalent metal cation 1 By similarity

Metal binding

798

Divalent metal cation 1 By similarity

Metal binding

798

Divalent metal cation 2 By similarity

Metal binding

913

Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue

273

Phosphoserine; by PKB/AKT1 or PKB/AKT2 Ref.1

Modified residue

421

Phosphoserine By similarity

Modified residue

604

Phosphothreonine Ref.4

Experimental info

Sequence conflict

A → T in CAA10639. Ref.1

Sequence conflict

261

E → D in CAA10639. Ref.1

Sequence conflict

288 – 290

SGK → IPE in CAA64775. Ref.3

Sequence conflict

439

S → G in CAA10639. Ref.1

Sequence conflict

444

Y → H in CAA10639. Ref.1

Sequence conflict

465

F → M in CAA10639. Ref.1

Sequence conflict

756

G → S in CAA10639. Ref.1

Sequence conflict

944

A → T in CAA10639. Ref.1

Sequence conflict

944

A → T in CAA64775. Ref.3

Sequence conflict

1082

A → T in CAA64775. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q61409 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 92E36B28D4A5FDF1
Show »

FASTA

1,100

122,154

        10         20         30         40         50         60 
MRKDERERDA PAMRSPPPPP ASAASPPESL RNGYVKSCVS PLRQDPPRSF FFHLCRFCNV 

        70         80         90        100        110        120 
EPPAASLRAG ARLSLGVLAA FVLAALLGAR PERWAAAAAG LRTLLSACSL SLSPLFSIAC 

       130        140        150        160        170        180 
AFFFLTCFLT RAQRGPGRGA GSWWLLALPA CCYLGDFAAW QWWSWLRGEP AAAGRLCLVL 

       190        200        210        220        230        240 
SCVGLLTLAP RVRLRHGVLV LLFAGLVWWV SFSGLGALPP ALRPLLSCLV GGAGCLLALG 

       250        260        270        280        290        300 
LDHFFHVRGA SPPPRSASTA EEKVPVIRPR RRSSCVSLGE SAAGYYGSGK MFRRPSLPCI 

       310        320        330        340        350        360 
SREQMILWDW DLKQWCKPHY QNSGGGNGVD LSVLNEARNM VSDLLIDPSL PPQVISSLRS 

       370        380        390        400        410        420 
ISSLMGAFSG SCRPKINSFT PFPGFYPCSE VEDPVEKGDR KLHKGLSGRT SFPTPQLRRS 

       430        440        450        460        470        480 
SGASSLLTNE HCSRWDRSSG KRSYQELSVS SHGCHLNGPF SSNLFTIPKQ RSSSVSLTHH 

       490        500        510        520        530        540 
AGLRRAGALP SHSLLNSSSH VPVSAGSLTN RSPIGFPDTT DFLTKPNIIL HRSLGSVSSA 

       550        560        570        580        590        600 
ADFHQYLRNS DSNLCSSCGH QILKYVSTCE PDGTDHPSEK SGEEDSSVFS KEPLNIVETQ 

       610        620        630        640        650        660 
EEETMKKACR ELFLEGDSHL MEEAQQPNID QEVSLDPMLV EDYDSLIEKM NNWNFQIFEL 

       670        680        690        700        710        720 
VEKMGEKSGR ILSQVMYTLF QDTGLLETFK IPTQEFMNYF RALENGYRDI PYHNRVHATD 

       730        740        750        760        770        780 
VLHAVWYLTT RPIPGLPQIH NNHETETKAD SDGRLGSGQI AYISSKSCCI PDMSYGCLSS 

       790        800        810        820        830        840 
NIPALELMAL YVAAAMHDYD HPGRTNAFLV ATNAPQAVLY NDRSVLENHH AASAWNLYLS 

       850        860        870        880        890        900 
RPEYNFLLNL DHMEFKRFRF LVIEAILATD LKKHFDFLAE FNAKANDVNS NGIEWSSEND 

       910        920        930        940        950        960 
RLLVCQVCIK LADINGPAKD RDLHLRWTEG IVNEFYEQGD EEAALGLPIS PFMDRSSPQL 

       970        980        990       1000       1010       1020 
AKLQESFITH IVGPLCNSYD AAGLLPGQWI ETEEGDDTES DDDDDDDDGD GGEELDSDDE 

      1030       1040       1050       1060       1070       1080 
ETEDNLNPKP QRRKGRRRIF CQLMHHLTEN HKIWKEIIEE EEEKCKAEGN KLQVDNASLP 

      1090       1100 
QADEIQVIEE ADEEEEQMFE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insulin-induced phosphorylation and activation of cyclic nucleotide phosphodiesterase 3B by the serine-threonine kinase Akt." Kitamura T., Kitamura Y., Kuroda S., Hino Y., Ando M., Kotani K., Konishi H., Matsuzaki H., Kikkawa U., Ogawa W., Kasuga M. Mol. Cell. Biol. 19:6286-6296(1999) [PubMed: 10454575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-273.
[2]	"Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide phosphodiesterase 3B (Pde3b) gene in differentiating 3T3-L1 preadipocytes." Liu H., Tang J.R., Choi Y.H., Napolitano M., Hockman S., Taira M., Degerman E., Manganiello V.C. J. Biol. Chem. 281:21096-21113(2006) [PubMed: 16702214] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: J125.
[3]	"Molecular cloning and chromosomal assignment of the human homologue of the rat cGMP-inhibited phosphodiesterase 1 (PDE3A) -- a gene involved in fat metabolism located at 11p15.1." Loebbert R.W., Winterpacht A., Seipel B., Zabel B.U. Genomics 37:211-218(1996) [PubMed: 8921398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 287-1085. Strain: Swiss. Tissue: Adipose tissue.
[4]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-604, MASS SPECTROMETRY. Tissue: Liver.
[5]	"PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects." Patrucco E., Notte A., Barberis L., Selvetella G., Maffei A., Brancaccio M., Marengo S., Russo G., Azzolino O., Rybalkin S.D., Silengo L., Altruda F., Wetzker R., Wymann M.P., Lembo G., Hirsch E. Cell 118:375-387(2004) [PubMed: 15294162] [Abstract] Cited for: INTERACTION WITH PIK3CG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132271 mRNA. Translation: CAA10639.1. AF547435 mRNA. Translation: AAN52086.1. X95521 mRNA. Translation: CAA64775.1.
IPI	IPI00719996.
RefSeq	NP_035185.2. NM_011055.2.
UniGene	Mm.430730.
3D structure databases
ProteinModelPortal	Q61409.
SMR	Q61409. Positions 632-1055.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61409.
Proteomic databases
PRIDE	Q61409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	18576.
KEGG	mmu:18576.
UCSC	uc009jic.1. mouse.
Organism-specific databases
CTD	5140.
MGI	MGI:1333863. Pde3b.
Phylogenomic databases
GeneTree	ENSGT00590000082978.
HOGENOM	HBG712991.
HOVERGEN	HBG053541.
InParanoid	Q61409.
OrthoDB	EOG4GB75F.
Enzyme and pathway databases
Reactome	REACT_115202. Signal Transduction. REACT_77193. Inhibition of HSL.
Gene expression databases
ArrayExpress	Q61409.
Bgee	Q61409.
CleanEx	MM_PDE3B.
Genevestigator	Q61409.
GermOnline	ENSMUSG00000030671. Mus musculus.
Family and domain databases
InterPro	IPR003607. Metal-dep_PHydrolase_HD_dom. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 3 hits.
KO	K13296.
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294434.
SOURCE	Search...

Entry information

Entry name

PDE3B_MOUSE

Accession

Primary (citable) accession number: Q61409
Secondary accession number(s): Q8CIX5, Q9Z1J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 9, 2007
Last modified:	November 16, 2011
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents