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Protein

Procollagen C-endopeptidase enhancer 1

Gene

Pcolce

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.

GO - Molecular functioni

GO - Biological processi

  • positive regulation of peptidase activity Source: MGI
  • proteolysis Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen C-endopeptidase enhancer 1
Alternative name(s):
P14
Procollagen COOH-terminal proteinase enhancer 1
Short name:
PCPE-1
Short name:
Procollagen C-proteinase enhancer 1
Type 1 procollagen C-proteinase enhancer protein
Type I procollagen COOH-terminal proteinase enhancer
Gene namesi
Name:Pcolce
Synonyms:Pcpe1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:105099. Pcolce.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 468444Procollagen C-endopeptidase enhancer 1PRO_0000022024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...)1 Publication
Disulfide bondi36 ↔ 62PROSITE-ProRule annotation
Modified residuei41 – 411PhosphothreonineBy similarity
Modified residuei49 – 491PhosphoserineBy similarity
Disulfide bondi89 ↔ 111PROSITE-ProRule annotation
Disulfide bondi158 ↔ 185PROSITE-ProRule annotation
Disulfide bondi212 ↔ 235PROSITE-ProRule annotation
Disulfide bondi341 ↔ 409By similarity
Disulfide bondi356 ↔ 460By similarity
Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Processed from a 55 kDa form to 36 kDa and 34 kDa forms.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ61398.
PaxDbiQ61398.
PRIDEiQ61398.

PTM databases

iPTMnetiQ61398.
PhosphoSiteiQ61398.

Miscellaneous databases

PMAP-CutDBQ61398.

Expressioni

Tissue specificityi

Expressed in interstitial connective tissues like tendons, calvaria, skin and at a lower level in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ61398.
CleanExiMM_PCOLCE.
ExpressionAtlasiQ61398. baseline and differential.
GenevisibleiQ61398. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031731.

Structurei

3D structure databases

ProteinModelPortaliQ61398.
SMRiQ61398. Positions 36-317, 333-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 148113CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini158 – 272115CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini341 – 460120NTRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IPS8. Eukaryota.
ENOG410Y9AW. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000059592.
HOVERGENiHBG053528.
InParanoidiQ61398.
OrthoDBiEOG7BCNBH.
PhylomeDBiQ61398.
TreeFamiTF316506.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR028870. PCOLCE.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERiPTHR10127:SF628. PTHR10127:SF628. 2 hits.
PfamiPF00431. CUB. 2 hits.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00042. CUB. 2 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50242. SSF50242. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS50189. NTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61398-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPAALTSFL GPFLLAWVLP LARGQTPNYT RPVFLCGGDV TGESGYVASE
60 70 80 90 100
GFPNLYPPNK KCIWTITVPE GQTVSLSFRV FDMELHPSCR YDALEVFAGS
110 120 130 140 150
GTSGQRLGRF CGTFRPAPVV APGNQVTLRM TTDEGTGGRG FLLWYSGRAT
160 170 180 190 200
SGTEHQFCGG RMEKAQGTLT TPNWPESDYP PGISCSWHII APSNQVIMLT
210 220 230 240 250
FGKFDVEPDT YCRYDSVSVF NGAVSDDSKR LGKFCGDKAP SPISSEGNEL
260 270 280 290 300
LVQFVSDLSV TADGFSASYR TLPRDAVEKE SALSPGEDVQ RGPQSRSDPK
310 320 330 340 350
TGTGPKVKPP TKPKSQPAET PEASPATQAT PVAPAAPSIT CPKQYKRSGT
360 370 380 390 400
LQSNFCSSSL VVTGTVKTMV RGPGEGLTVT VSLLGVYKTG GLDLPSPPSG
410 420 430 440 450
TSLKLYVPCR QMPPMKKGAS YLLMGQVEEN RGPILPPESF VVLYRSNQDQ
460
ILNNLSKRKC PSQPRTAA
Length:468
Mass (Da):50,168
Last modified:May 30, 2000 - v2
Checksum:iF9B55558147FABAB
GO

Sequence cautioni

The sequence CAA40612.1 differs from that shown. Reason: Frameshift at positions 391 and 445. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781D → Y in CAA40612 (PubMed:2027019).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008548 mRNA. Translation: BAA23280.1.
X57337 mRNA. Translation: CAA40612.1. Frameshift.
CCDSiCCDS39334.1.
PIRiJH0403.
RefSeqiNP_032814.2. NM_008788.2.
UniGeneiMm.262345.

Genome annotation databases

EnsembliENSMUST00000031731; ENSMUSP00000031731; ENSMUSG00000029718.
GeneIDi18542.
KEGGimmu:18542.
UCSCiuc009adb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008548 mRNA. Translation: BAA23280.1.
X57337 mRNA. Translation: CAA40612.1. Frameshift.
CCDSiCCDS39334.1.
PIRiJH0403.
RefSeqiNP_032814.2. NM_008788.2.
UniGeneiMm.262345.

3D structure databases

ProteinModelPortaliQ61398.
SMRiQ61398. Positions 36-317, 333-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031731.

PTM databases

iPTMnetiQ61398.
PhosphoSiteiQ61398.

Proteomic databases

MaxQBiQ61398.
PaxDbiQ61398.
PRIDEiQ61398.

Protocols and materials databases

DNASUi18542.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031731; ENSMUSP00000031731; ENSMUSG00000029718.
GeneIDi18542.
KEGGimmu:18542.
UCSCiuc009adb.1. mouse.

Organism-specific databases

CTDi5118.
MGIiMGI:105099. Pcolce.

Phylogenomic databases

eggNOGiENOG410IPS8. Eukaryota.
ENOG410Y9AW. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000059592.
HOVERGENiHBG053528.
InParanoidiQ61398.
OrthoDBiEOG7BCNBH.
PhylomeDBiQ61398.
TreeFamiTF316506.

Enzyme and pathway databases

ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

ChiTaRSiPcolce. mouse.
NextBioi294324.
PMAP-CutDBQ61398.
PROiQ61398.
SOURCEiSearch...

Gene expression databases

BgeeiQ61398.
CleanExiMM_PCOLCE.
ExpressionAtlasiQ61398. baseline and differential.
GenevisibleiQ61398. MM.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR028870. PCOLCE.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERiPTHR10127:SF628. PTHR10127:SF628. 2 hits.
PfamiPF00431. CUB. 2 hits.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00042. CUB. 2 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50242. SSF50242. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Type I procollagen COOH-terminal proteinase enhancer protein: identification, primary structure, and chromosomal localization of the cognate human gene (PCOLCE)."
    Takahara K., Kessler E., Biniaminov L., Brusel M., Eddy R.L., Jani-Sait S., Shows T.B., Greenspan D.S.
    J. Biol. Chem. 269:26280-26285(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Smooth muscle cell derived procollagen C-protease enhancer protein."
    Hirahara I., Syoufuda K., Harada K., Tomita M., Urakami K., Terai H., Morisaki N., Saito Y.
    Cell Struct. Funct. 21:662-662(1996)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Heart.
  3. "Isolation of a novel cDNA corresponding to a transcript expressed in the choroid plexus and leptomeninges."
    Lecain E., Zelenika D., Laine M.C., Rhyner T., Pessac B.
    J. Neurochem. 56:2133-2138(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: CNS.
  4. "Type I procollagen C-proteinase from mouse fibroblasts. Purification and demonstration of a 55-kDa enhancer glycoprotein."
    Kessler E., Adar R.
    Eur. J. Biochem. 186:115-121(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Procollagen type I C-proteinase enhancer is a naturally occurring connective tissue glycoprotein."
    Kessler E., Mould A.P., Hulmes D.J.S.
    Biochem. Biophys. Res. Commun. 173:81-86(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
    Strain: ICR.
  6. Kessler E.
    Unpublished observations (JAN-2000)
    Cited for: GLYCOSYLATION AT ASN-28.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung.

Entry informationi

Entry nameiPCOC1_MOUSE
AccessioniPrimary (citable) accession number: Q61398
Secondary accession number(s): O35113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: January 20, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.