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Protein

TNF receptor-associated factor 4

Gene

Traf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions (By similarity). Required for normal skeleton development, and for normal development of the respiratory tract.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5841RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri101 – 15454TRAF-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri155 – 20854TRAF-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri209 – 26759TRAF-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
TNF receptor-associated factor 4
Alternative name(s):
Cysteine-rich motif associated to RING and Traf domains protein 1
Gene namesi
Name:Traf4
Synonyms:Cart1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1202880. Traf4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Tight junction

Pathology & Biotechi

Disruption phenotypei

Mice exhibit considerable phenotypic variability, depending in part on the strain. In one strain pups are born at the expected Mendelian frequency, while in another strain up to one third die during embryogenesis. Mutants that reach adulthood are fertile, but have on average three pups per litter instead of ten in wild-type. Mutants have an apparently normal immune response, with no defects in the development of T and B-lymphocytes, granulocytes, macrophages and dendritic cells. Mutants have respiratory problems, due to developmental defects of the trachea, stem bronchi and rib cage. They exhibit severe skeletal alterations at the level of the spinal column, including scoliosis and kyphosis, and have curly tails. Many also display neural tube closure defects.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470TNF receptor-associated factor 4PRO_0000056404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei426 – 4261PhosphoserineCombined sources

Post-translational modificationi

Polyubiquitinated, leading to its proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61382.
MaxQBiQ61382.
PaxDbiQ61382.
PeptideAtlasiQ61382.
PRIDEiQ61382.

PTM databases

iPTMnetiQ61382.
PhosphoSiteiQ61382.

Expressioni

Tissue specificityi

Predominantly expressed in brain. Preferentially expressed by post-mitotic undifferentiated neurons in developing central (CNS) and peripheral (PNS) nervous system, and in nervous tissues of sensory organs. In the embryo, protein expression was shown in brain, thymus, salivary glands and intestine. In the adult, protein expression is restricted to the brain (hippocampus and olfactory bulb).1 Publication

Developmental stagei

Strongly expressed throughout embryogenesis with a maximum from 8.5 to 13.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000017386.
CleanExiMM_TRAF4.
GenevisibleiQ61382. MM.

Interactioni

Subunit structurei

Homotrimer (Probable). Interacts with LTBR/TNFRSF3, NGFR/TNFRSF16, RPS6KB1 and TGFB1I1. Interacts with SMURF1. Interacts (via TRAF domain) with MAP3K4 (via kinase domain). Interacts with NCF1, TICAM1, IRAK1 and TRAF6, and is probably part of a complex containing TRAF4, NCF1, TICAM1, IRAK1 and TRAF6 (By similarity).By similarityCurated

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204305. 3 interactions.
STRINGi10090.ENSMUSP00000017530.

Structurei

3D structure databases

ProteinModelPortaliQ61382.
SMRiQ61382. Positions 3-164, 190-248, 291-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini307 – 462156MATHPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili277 – 31034Sequence analysisAdd
BLAST

Domaini

The coiled coil domain mediates homo- and hetero-oligomerization.By similarity
The MATH/TRAF domain binds to receptor cytoplasmic domains.By similarity

Sequence similaritiesi

Contains 1 MATH domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 3 TRAF-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5841RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri101 – 15454TRAF-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri155 – 20854TRAF-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri209 – 26759TRAF-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0297. Eukaryota.
ENOG410Z7YX. LUCA.
GeneTreeiENSGT00550000074359.
HOGENOMiHOG000004841.
HOVERGENiHBG103438.
InParanoidiQ61382.
KOiK09848.
OMAiYESHEGV.
OrthoDBiEOG091G0GHD.
TreeFamiTF321154.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
3.30.40.10. 1 hit.
3.90.890.10. 1 hit.
InterProiIPR002083. MATH/TRAF_dom.
IPR013323. SIAH-type.
IPR012227. TNF_rcpt--assoc_TRAF.
IPR008974. TRAF-like.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
IPR001293. Znf_TRAF.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
PF02176. zf-TRAF. 2 hits.
[Graphical view]
PIRSFiPIRSF015614. TRAF. 1 hit.
SMARTiSM00061. MATH. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 4 hits.
PROSITEiPS50144. MATH. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
PS50145. ZF_TRAF. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGFDYKFLE KPKRRLLCPL CGKPMREPVQ VSTCGHRFCD TCLQEFLSEG
60 70 80 90 100
VFKCPEDQLP LDYAKIYPDP ELEVQVLGLA IRCIHSEEGC RWSGPLRHLQ
110 120 130 140 150
GHLNTCSFNV VPCPNRCPAK LSRRDLPAHL QHDCPKRRLK CEFCGCDFSG
160 170 180 190 200
EAYESHEGVC PQESVYCENK CGARMMRRLL AQHATSECPK RTQPCAYCTK
210 220 230 240 250
EFVYDTIQSH QYQCPRLPVP CPNQCGVGTV AREDLPTHLK DSCSTAFVLC
260 270 280 290 300
PFKESGCKHR CPKLAMGRHV EESVKPHLAM MCALVSRQRQ ELQELRRELE
310 320 330 340 350
ELSIGSDGVL IWKIGSYGRR LQEAKAKPNL ECFSPAFYTH KYGYKLQVSA
360 370 380 390 400
FLNGNGSGEG THLSIYIRVL PGAFDNLLEW PFARRVTFSL LDQSDPGLAK
410 420 430 440 450
PQHVTETFHP DPNWKNFQKP GTWRGSLDES SLGFGYPKFI SHQDIRKRNY
460 470
VRDDAVFIRA SVELPRKILS
Length:470
Mass (Da):53,504
Last modified:July 27, 2011 - v2
Checksum:i2C99A876E98B99C5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41F → L in CAA63103 (PubMed:9507120).Curated
Sequence conflicti9 – 91L → P in CAA63103 (PubMed:9507120).Curated
Sequence conflicti27 – 304EPVQ → DSVE in CAA63103 (PubMed:9507120).Curated
Sequence conflicti38 – 381F → Y in CAA63103 (PubMed:9507120).Curated
Sequence conflicti41 – 411T → N in CAA63103 (PubMed:9507120).Curated
Sequence conflicti166 – 1661Y → F in CAA63103 (PubMed:9507120).Curated
Sequence conflicti244 – 2441S → R in CAA63103 (PubMed:9507120).Curated
Sequence conflicti351 – 3511F → S in CAA63103 (PubMed:9507120).Curated
Sequence conflicti382 – 3821F → L in CAA63103 (PubMed:9507120).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92346 mRNA. Translation: CAA63103.1.
AF233449 mRNA. Translation: AAF44757.1.
AK035056 mRNA. Translation: BAC28928.1.
AK040877 mRNA. Translation: BAC30729.1.
AK053900 mRNA. Translation: BAC35582.1.
AL591070 Genomic DNA. Translation: CAI24336.1.
CH466596 Genomic DNA. Translation: EDL12913.1.
CCDSiCCDS25090.1.
RefSeqiNP_033449.2. NM_009423.4.
UniGeneiMm.390418.

Genome annotation databases

EnsembliENSMUST00000017530; ENSMUSP00000017530; ENSMUSG00000017386.
GeneIDi22032.
KEGGimmu:22032.
UCSCiuc007kif.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92346 mRNA. Translation: CAA63103.1.
AF233449 mRNA. Translation: AAF44757.1.
AK035056 mRNA. Translation: BAC28928.1.
AK040877 mRNA. Translation: BAC30729.1.
AK053900 mRNA. Translation: BAC35582.1.
AL591070 Genomic DNA. Translation: CAI24336.1.
CH466596 Genomic DNA. Translation: EDL12913.1.
CCDSiCCDS25090.1.
RefSeqiNP_033449.2. NM_009423.4.
UniGeneiMm.390418.

3D structure databases

ProteinModelPortaliQ61382.
SMRiQ61382. Positions 3-164, 190-248, 291-468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204305. 3 interactions.
STRINGi10090.ENSMUSP00000017530.

PTM databases

iPTMnetiQ61382.
PhosphoSiteiQ61382.

Proteomic databases

EPDiQ61382.
MaxQBiQ61382.
PaxDbiQ61382.
PeptideAtlasiQ61382.
PRIDEiQ61382.

Protocols and materials databases

DNASUi22032.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017530; ENSMUSP00000017530; ENSMUSG00000017386.
GeneIDi22032.
KEGGimmu:22032.
UCSCiuc007kif.2. mouse.

Organism-specific databases

CTDi9618.
MGIiMGI:1202880. Traf4.

Phylogenomic databases

eggNOGiKOG0297. Eukaryota.
ENOG410Z7YX. LUCA.
GeneTreeiENSGT00550000074359.
HOGENOMiHOG000004841.
HOVERGENiHBG103438.
InParanoidiQ61382.
KOiK09848.
OMAiYESHEGV.
OrthoDBiEOG091G0GHD.
TreeFamiTF321154.

Miscellaneous databases

PROiQ61382.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017386.
CleanExiMM_TRAF4.
GenevisibleiQ61382. MM.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
3.30.40.10. 1 hit.
3.90.890.10. 1 hit.
InterProiIPR002083. MATH/TRAF_dom.
IPR013323. SIAH-type.
IPR012227. TNF_rcpt--assoc_TRAF.
IPR008974. TRAF-like.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
IPR001293. Znf_TRAF.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
PF02176. zf-TRAF. 2 hits.
[Graphical view]
PIRSFiPIRSF015614. TRAF. 1 hit.
SMARTiSM00061. MATH. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 4 hits.
PROSITEiPS50144. MATH. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
PS50145. ZF_TRAF. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRAF4_MOUSE
AccessioniPrimary (citable) accession number: Q61382
Secondary accession number(s): Q8BHD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.