Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Intraflagellar transport protein 88 homolog

Gene

Ift88

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.2 Publications

GO - Molecular functioni

  • kinesin binding Source: MGI

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • brain development Source: MGI
  • cardiac muscle cell differentiation Source: MGI
  • cardiac septum morphogenesis Source: MGI
  • cilium assembly Source: MGI
  • cilium morphogenesis Source: UniProtKB
  • cochlea development Source: MGI
  • cytoplasmic microtubule organization Source: MGI
  • determination of left/right symmetry Source: MGI
  • dorsal/ventral pattern formation Source: MGI
  • embryonic digit morphogenesis Source: MGI
  • epidermal stem cell homeostasis Source: MGI
  • epidermis development Source: MGI
  • epithelial cell morphogenesis Source: MGI
  • eye development Source: MGI
  • forebrain morphogenesis Source: MGI
  • heart development Source: MGI
  • heart formation Source: MGI
  • inner ear receptor stereocilium organization Source: MGI
  • kidney development Source: MGI
  • limb development Source: MGI
  • liver development Source: MGI
  • lung development Source: MGI
  • lung vasculature development Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • nervous system development Source: MGI
  • neural precursor cell proliferation Source: MGI
  • nonmotile primary cilium assembly Source: MGI
  • Notch signaling pathway Source: MGI
  • organ morphogenesis Source: MGI
  • palate development Source: MGI
  • pancreas development Source: MGI
  • positive regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  • protein localization Source: MGI
  • regulation of autophagosome assembly Source: UniProtKB
  • regulation of cilium assembly Source: UniProtKB
  • regulation of fat cell differentiation Source: MGI
  • regulation of feeding behavior Source: MGI
  • regulation of odontogenesis of dentin-containing tooth Source: MGI
  • regulation of protein processing Source: MGI
  • regulation of smoothened signaling pathway Source: MGI
  • response to fluid shear stress Source: MGI
  • smoothened signaling pathway Source: MGI
  • spermatid nucleus elongation Source: MGI
  • sperm axoneme assembly Source: MGI
  • spinal cord dorsal/ventral patterning Source: MGI
  • telencephalon development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Intraflagellar transport protein 88 homolog
Alternative name(s):
Recessive polycystic kidney disease protein Tg737
Tetratricopeptide repeat protein 10
Short name:
TPR repeat protein 10
TgN(Imorpk)737Rpw
Gene namesi
Name:Ift88
Synonyms:Tg737, Tg737Rpw, TgN737Rpw, Ttc10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98715. Ift88.

Subcellular locationi

GO - Cellular componenti

  • acrosomal membrane Source: MGI
  • apical part of cell Source: BHF-UCL
  • axonemal basal plate Source: MGI
  • axoneme Source: BHF-UCL
  • centriole Source: UniProtKB
  • centrosome Source: MGI
  • ciliary basal body Source: UniProtKB
  • ciliary base Source: MGI
  • ciliary tip Source: MGI
  • cilium Source: UniProtKB
  • intraciliary transport particle B Source: UniProtKB
  • kinocilium Source: MGI
  • motile primary cilium Source: BHF-UCL
  • outer acrosomal membrane Source: MGI
  • photoreceptor connecting cilium Source: UniProtKB
  • primary cilium Source: BHF-UCL
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Involvement in diseasei

Defects in Ift88 are the cause of recessive bilateral polycystic kidney disease (PKD) with collecting duct and tubule ectasia, and a liver lesion involving biliary dysplasia and/or portal fibrosis.

Disruption phenotypei

Cilia absent or reduced, virtually no cilia of the normal 5 µM mean length.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 824824Intraflagellar transport protein 88 homologPRO_0000106392Add
BLAST

Proteomic databases

MaxQBiQ61371.
PaxDbiQ61371.
PRIDEiQ61371.

PTM databases

iPTMnetiQ61371.
PhosphoSiteiQ61371.

Expressioni

Gene expression databases

BgeeiQ61371.

Interactioni

Subunit structurei

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT20, IFT22, HSPB11, IFT27, IFT46, IFT52, TRAF3IP1, IFT57, IFT74, IFT80, IFT81, IFT88 AND IFT172. Interacts with C2CD3.4 Publications

GO - Molecular functioni

  • kinesin binding Source: MGI

Protein-protein interaction databases

BioGridi204171. 13 interactions.
STRINGi10090.ENSMUSP00000113768.

Structurei

3D structure databases

ProteinModelPortaliQ61371.
SMRiQ61371. Positions 199-330, 420-716.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati196 – 22934TPR 1Add
BLAST
Repeati232 – 26534TPR 2Add
BLAST
Repeati271 – 30434TPR 3Add
BLAST
Repeati415 – 44834TPR 4Add
BLAST
Repeati450 – 48334TPR 5Add
BLAST
Repeati484 – 51734TPR 6Add
BLAST
Repeati518 – 55134TPR 7Add
BLAST
Repeati552 – 58534TPR 8Add
BLAST
Repeati586 – 61934TPR 9Add
BLAST
Repeati620 – 65334TPR 10Add
BLAST
Repeati654 – 68734TPR 11Add
BLAST

Sequence similaritiesi

Contains 11 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG2003. Eukaryota.
ENOG410XPPW. LUCA.
HOGENOMiHOG000258203.
HOVERGENiHBG059328.
InParanoidiQ61371.
KOiK16474.
OMAiMTKNKMF.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13174. TPR_6. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 11 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 10 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENVHLAPET DEDDLYSGFN DYNPAYDTEE LENDTGFQQA VRTSHGRRPP
60 70 80 90 100
VTAKIPSTAV SRPIATGYGS KTSLTSSMGR PMTGTIQDGV ARPMTAVRAA
110 120 130 140 150
GFSKAALRGS AFDPLGQSRG PAPPLEAKNE DSPEEKIRQL EKKVNELVEE
160 170 180 190 200
SCIANSCGDL KLALEKAKDA GRKERVLVRQ REQVTSPENI NLDLTYSVLF
210 220 230 240 250
NLASQYSANE MYAEALNTYQ VIVKNKMFSN AGRLKVNMGN IYLKQRNYSK
260 270 280 290 300
AIKFYRMALD QIPSVHKEMR IKIMQNIGIT FIKTGQYSDA INSFEHIMSM
310 320 330 340 350
APSLKAGFNL ILSCFAIGDR EKMKKAFQKL IAVPLEIDED DKYISPSDDP
360 370 380 390 400
HTNLLIEAIK NDHLRQMERE RKAMAEKYIM TAAKLIAPVI EASFAVGYNW
410 420 430 440 450
CVEVVKASQY VELANDLEIN KAITYLRQKD FNQAVDTLKM FEKKDSRVKS
460 470 480 490 500
AAATNLSFLY YLENEFAQAS SYADLAVNSD RYNPSALTNK GNTVFANGDY
510 520 530 540 550
EKAAEFYKEA LRNDSSCTEA LYNIGLTYKK LNRLDEALDS FLKLHAILRN
560 570 580 590 600
SAQVLCQIAN IYELMEDPNQ AIEWLMQLIS VVPTDSQALS KLGELYDSEG
610 620 630 640 650
DKSQAFQYYY ESYRYFPSNI EVIEWLGAYY IDTQFCEKAI QYFERASLIQ
660 670 680 690 700
PTQVKWQLMV ASCFRRSGNY QKALDTYKEI HRKFPENVEC LRFLVRLCTD
710 720 730 740 750
IGLKEVQEYA TKLKRLEKMK EMREQRIKSG RDSSGGSRSK REGSAGSDSG
760 770 780 790 800
QNNSASSKSE RLSAKLRALP GTDEPYESSG NKEIDASYVD PLGPQIERPK
810 820
TAAKKRIDED DFADEELGDD LLPE
Length:824
Mass (Da):92,994
Last modified:July 27, 2011 - v2
Checksum:iBC239BA4B8FE3B5D
GO

Sequence cautioni

The sequence BAE22424.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641I → T in AAH12250 (PubMed:15489334).Curated
Sequence conflicti242 – 2421Y → H in AAH12250 (PubMed:15489334).Curated
Sequence conflicti459 – 4591L → M in BAE22424 (PubMed:16141072).Curated
Sequence conflicti700 – 7001D → E in AAH12250 (PubMed:15489334).Curated
Sequence conflicti823 – 8231P → S in AAB59705 (PubMed:8191288).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31959 mRNA. Translation: AAB59705.1.
AK135110 mRNA. Translation: BAE22424.1. Different initiation.
AC124462 Genomic DNA. No translation available.
AC154675 Genomic DNA. No translation available.
BC012250 mRNA. Translation: AAH12250.1.
PIRiI49564.
RefSeqiNP_033402.2. NM_009376.2.
UniGeneiMm.4653.

Genome annotation databases

GeneIDi21821.
KEGGimmu:21821.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31959 mRNA. Translation: AAB59705.1.
AK135110 mRNA. Translation: BAE22424.1. Different initiation.
AC124462 Genomic DNA. No translation available.
AC154675 Genomic DNA. No translation available.
BC012250 mRNA. Translation: AAH12250.1.
PIRiI49564.
RefSeqiNP_033402.2. NM_009376.2.
UniGeneiMm.4653.

3D structure databases

ProteinModelPortaliQ61371.
SMRiQ61371. Positions 199-330, 420-716.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204171. 13 interactions.
STRINGi10090.ENSMUSP00000113768.

PTM databases

iPTMnetiQ61371.
PhosphoSiteiQ61371.

Proteomic databases

MaxQBiQ61371.
PaxDbiQ61371.
PRIDEiQ61371.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21821.
KEGGimmu:21821.

Organism-specific databases

CTDi8100.
MGIiMGI:98715. Ift88.

Phylogenomic databases

eggNOGiKOG2003. Eukaryota.
ENOG410XPPW. LUCA.
HOGENOMiHOG000258203.
HOVERGENiHBG059328.
InParanoidiQ61371.
KOiK16474.
OMAiMTKNKMF.

Miscellaneous databases

ChiTaRSiIft88. mouse.
NextBioi301236.
PROiQ61371.
SOURCEiSearch...

Gene expression databases

BgeeiQ61371.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13174. TPR_6. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 11 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 10 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Candidate gene associated with a mutation causing recessive polycystic kidney disease in mice."
    Moyer J.H., Lee-Tischler M.J., Kwon H.-Y., Schrick J.J., Avner E.D., Sweeney W.E., Godfrey V.L., Cacheiro N.L., Wilkinson J.E., Woychik R.P.
    Science 264:1329-1333(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INVOLVEMENT IN PKD.
    Strain: C3HF/RL.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Olfactory bulb.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  5. "Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella."
    Pazour G.J., Dickert B.L., Vucica Y., Seeley E.S., Rosenbaum J.L., Witman G.B., Cole D.G.
    J. Cell Biol. 151:709-718(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH IFT57 AND IFT172.
  6. "Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella."
    Hou Y., Qin H., Follit J.A., Pazour G.J., Rosenbaum J.L., Witman G.B.
    J. Cell Biol. 176:653-665(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IFT46.
  7. Cited for: IDENTIFICATION IN THE IFT COMPLEX B, INTERACTION WITH IFT20; IFT22; HSPB11; IFT27; IFT46; IFT52; TRAF3IP1; IFT57; IFT74; IFT80; IFT81; IFT88, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  9. "Ofd1, a human disease gene, regulates the length and distal structure of centrioles."
    Singla V., Romaguera-Ros M., Garcia-Verdugo J.M., Reiter J.F.
    Dev. Cell 18:410-424(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Functional characterization of putative cilia genes by high-content analysis."
    Lai C.K., Gupta N., Wen X., Rangell L., Chih B., Peterson A.S., Bazan J.F., Li L., Scales S.J.
    Mol. Biol. Cell 22:1104-1119(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  11. Cited for: SUBCELLULAR LOCATION.
  12. "C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals."
    Ye X., Zeng H., Ning G., Reiter J.F., Liu A.
    Proc. Natl. Acad. Sci. U.S.A. 111:2164-2169(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH C2CD3.

Entry informationi

Entry nameiIFT88_MOUSE
AccessioniPrimary (citable) accession number: Q61371
Secondary accession number(s): E9QKU0, Q3UXY9, Q921J5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.