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Protein

Chitinase-3-like protein 1

Gene

Chi3l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei150ChitooligosaccharideBy similarity1
Binding sitei361ChitooligosaccharideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial

Keywords - Biological processi

Apoptosis, Inflammatory response

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase-3-like protein 1
Alternative name(s):
BRP39 protein
Breast regression protein 39
Cartilage glycoprotein 39
Short name:
CGP-39
Short name:
GP-39
Gene namesi
Name:Chi3l1
Synonyms:Brp39, Chil1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1340899. Chil1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular region Source: GO_Central
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice are viable, fertile and appear normal, but have defective antigen-induced Th2 inflammatory responses and defective IL-13-induced responses, displaying accelerated cell death responses and diminished M2 macrophage differentiation. Mutant mice are more sensitive to S.pneumoniae infection, displaying enhanced mortality, exacerbated lung injury and decreased bacterial clearance compared to wild-type mice. Mutant mice also have an exacerbated response to hyperoxia, displaying enhanced protein leak, tissue inflammation and chemokine production and premature death.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136L → E: No effect on chiting-binding. No restoration of chitinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29By similarityAdd BLAST29
ChainiPRO_000001196630 – 389Chitinase-3-like protein 1Add BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi34 ↔ 59By similarity
Glycosylationi68N-linked (GlcNAc...)By similarity1
Disulfide bondi309 ↔ 372By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ61362.
PeptideAtlasiQ61362.
PRIDEiQ61362.

PTM databases

PhosphoSitePlusiQ61362.

Expressioni

Tissue specificityi

Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon.4 Publications

Inductioni

Up-regulated in colon under several inflammatory conditions. Up-regulated upon pulmonary inflammation elicited by sensitization and challenge with the chitin-free aeroallergen ovalbumin or with chitin-containing antigen house dust mite (HDM) extract. Up-regulated in lungs after S.pneumoniae infection. Up-regulated in splenic cells of mammary tumor-bearing animals. Down-regulated by hyperoxia in lung.5 Publications

Gene expression databases

BgeeiENSMUSG00000064246.
CleanExiMM_CHI3L1.
ExpressionAtlasiQ61362. baseline and differential.
GenevisibleiQ61362. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ61362. 1 interactor.
MINTiMINT-4090719.
STRINGi10090.ENSMUSP00000080717.

Structurei

3D structure databases

ProteinModelPortaliQ61362.
SMRiQ61362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 80Chitooligosaccharide bindingBy similarity2
Regioni106 – 109Chitooligosaccharide bindingBy similarity4
Regioni213 – 216Chitooligosaccharide bindingBy similarity4
Regioni333 – 347Important for AKT1 activation and IL8 productionAdd BLAST15

Sequence similaritiesi

Belongs to the glycosyl hydrolase 18 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
GeneTreeiENSGT00550000074323.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiQ61362.
KOiK17523.
OMAiSNTERRT.
OrthoDBiEOG091G014W.
TreeFamiTF315610.

Family and domain databases

Gene3Di3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028538. CHI3L1.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11177:SF202. PTHR11177:SF202. 1 hit.
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q61362-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTSTEARMG MRAALTGFAV LMLLQSCSAY KLVCYFTSWS QYREGVGSFL
60 70 80 90 100
PDAIQPFLCT HIIYSFANIS SDNMLSTWEW NDESNYDKLN KLKTRNTNLK
110 120 130 140 150
TLLSVGGWKF GEKRFSEIAS NTERRTAFVR SVAPFLRSYG FDGLDLAWLY
160 170 180 190 200
PRLRDKQYFS TLIKELNAEF TKEVQPGREK LLLSAALSAG KVAIDTGYDI
210 220 230 240 250
AQIAQHLDFI NLMTYDFHGV WRQITGHHSP LFQGQKDTRF DRYSNVNYAV
260 270 280 290 300
QYMIRLGAQA SKLLMGIPTF GKSFTLASSE NQLGAPISGE GLPGRFTKEA
310 320 330 340 350
GTLAYYEICD FLKGAEVHRL SNEKVPFATK GNQWVGYEDK ESVKNKVGFL
360 370 380
KEKKLAGAMV WALDLDDFQG TCQPKEFFPL TNAIKDALA
Length:389
Mass (Da):43,893
Last modified:May 14, 2014 - v3
Checksum:i9E7D66069B233834
GO
Isoform 2 (identifier: Q61362-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Note: Produced by alternative initiation at Met-9 of isoform 1.
Show »
Length:381
Mass (Da):42,979
Checksum:iEF6588C5AE9D4450
GO
Isoform 3 (identifier: Q61362-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MHTSTEARMGMRAALT → MTLQLA

Note: May be produced by alternative splicing of isoform 1. Gene prediction based on EST data.
Show »
Length:379
Mass (Da):42,805
Checksum:i289A0DCD41EE5E9D
GO

Sequence cautioni

The sequence AAH03780 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH04734 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH05611 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA63603 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112E → G in ABY53363 (Ref. 4) Curated1
Sequence conflicti258A → V in BAE33251 (PubMed:16141072).Curated1
Sequence conflicti339D → H in CAA63603 (PubMed:7970700).Curated1
Sequence conflicti348G → R in ABY53363 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0545231 – 16MHTST…RAALT → MTLQLA in isoform 3. CuratedAdd BLAST16
Alternative sequenceiVSP_0545241 – 8Missing in isoform 2. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93035 mRNA. Translation: CAA63603.1. Different initiation.
EU313768 mRNA. Translation: ABY53363.1.
AK051475 mRNA. Translation: BAC34654.1.
AK155412 mRNA. Translation: BAE33251.1.
CT010283 mRNA. Translation: CAJ18491.1.
AC137104 Genomic DNA. No translation available.
BC003780 mRNA. Translation: AAH03780.1. Different initiation.
BC004734 mRNA. Translation: AAH04734.1. Different initiation.
BC005611 mRNA. Translation: AAH05611.1. Different initiation.
CCDSiCCDS48368.1. [Q61362-1]
PIRiS61551.
RefSeqiNP_031721.2. NM_007695.3. [Q61362-1]
XP_006529174.1. XM_006529111.3. [Q61362-3]
UniGeneiMm.38274.

Genome annotation databases

EnsembliENSMUST00000082060; ENSMUSP00000080717; ENSMUSG00000064246. [Q61362-1]
ENSMUST00000153856; ENSMUSP00000117117; ENSMUSG00000064246. [Q61362-2]
ENSMUST00000156873; ENSMUSP00000119205; ENSMUSG00000064246. [Q61362-3]
GeneIDi12654.
KEGGimmu:12654.
UCSCiuc007crg.2. mouse. [Q61362-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93035 mRNA. Translation: CAA63603.1. Different initiation.
EU313768 mRNA. Translation: ABY53363.1.
AK051475 mRNA. Translation: BAC34654.1.
AK155412 mRNA. Translation: BAE33251.1.
CT010283 mRNA. Translation: CAJ18491.1.
AC137104 Genomic DNA. No translation available.
BC003780 mRNA. Translation: AAH03780.1. Different initiation.
BC004734 mRNA. Translation: AAH04734.1. Different initiation.
BC005611 mRNA. Translation: AAH05611.1. Different initiation.
CCDSiCCDS48368.1. [Q61362-1]
PIRiS61551.
RefSeqiNP_031721.2. NM_007695.3. [Q61362-1]
XP_006529174.1. XM_006529111.3. [Q61362-3]
UniGeneiMm.38274.

3D structure databases

ProteinModelPortaliQ61362.
SMRiQ61362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61362. 1 interactor.
MINTiMINT-4090719.
STRINGi10090.ENSMUSP00000080717.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

PTM databases

PhosphoSitePlusiQ61362.

Proteomic databases

PaxDbiQ61362.
PeptideAtlasiQ61362.
PRIDEiQ61362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082060; ENSMUSP00000080717; ENSMUSG00000064246. [Q61362-1]
ENSMUST00000153856; ENSMUSP00000117117; ENSMUSG00000064246. [Q61362-2]
ENSMUST00000156873; ENSMUSP00000119205; ENSMUSG00000064246. [Q61362-3]
GeneIDi12654.
KEGGimmu:12654.
UCSCiuc007crg.2. mouse. [Q61362-1]

Organism-specific databases

CTDi12654.
MGIiMGI:1340899. Chil1.

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
GeneTreeiENSGT00550000074323.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiQ61362.
KOiK17523.
OMAiSNTERRT.
OrthoDBiEOG091G014W.
TreeFamiTF315610.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ61362.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064246.
CleanExiMM_CHI3L1.
ExpressionAtlasiQ61362. baseline and differential.
GenevisibleiQ61362. MM.

Family and domain databases

Gene3Di3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028538. CHI3L1.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11177:SF202. PTHR11177:SF202. 1 hit.
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCH3L1_MOUSE
AccessioniPrimary (citable) accession number: Q61362
Secondary accession number(s): B0FEU7
, D3Z2P2, Q3U291, Q4FJW9, Q8BKL8, Q99J84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 14, 2014
Last modified: November 30, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although it belongs to the glycosyl hydrolase 18 family, Leu-149 is present instead of the conserved Glu which is an active site residue. Therefore this protein lacks chitinase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.