UniProtKB - Q61337 (BAD_MOUSE)
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Protein
Bcl2-associated agonist of cell death
Gene
Bad
Organism
Mus musculus (Mouse)
Status
Functioni
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
GO - Molecular functioni
- 14-3-3 protein binding Source: Ensembl
- cysteine-type endopeptidase activator activity involved in apoptotic process Source: UniProtKB
- lipid binding Source: UniProtKB
- phospholipid binding Source: UniProtKB
- protein heterodimerization activity Source: MGI
- protein kinase B binding Source: Ensembl
- protein kinase binding Source: MGI
- protein phosphatase 2B binding Source: Ensembl
- protein phosphatase binding Source: MGI
GO - Biological processi
- activation of cysteine-type endopeptidase activity Source: MGI
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- ADP metabolic process Source: UniProtKB
- apoptotic process Source: MGI
- apoptotic signaling pathway Source: MGI
- ATP metabolic process Source: UniProtKB
- cell proliferation Source: MGI
- cellular process regulating host cell cycle in response to virus Source: MGI
- cellular response to chromate Source: Ensembl
- cellular response to hypoxia Source: Ensembl
- cellular response to lipid Source: UniProtKB
- cellular response to mechanical stimulus Source: Ensembl
- cellular response to nicotine Source: UniProtKB
- cellular response to organic substance Source: MGI
- cerebral cortex development Source: Ensembl
- cytokine-mediated signaling pathway Source: MGI
- extrinsic apoptotic signaling pathway Source: MGI
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
- glucose catabolic process Source: MGI
- glucose homeostasis Source: UniProtKB
- intrinsic apoptotic signaling pathway Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
- negative regulation of cytolysis Source: MGI
- pore complex assembly Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of apoptotic process by virus Source: MGI
- positive regulation of B cell differentiation Source: MGI
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- positive regulation of epithelial cell proliferation Source: UniProtKB
- positive regulation of glucokinase activity Source: UniProtKB
- positive regulation of insulin secretion Source: UniProtKB
- positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
- positive regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Source: CAFA
- positive regulation of mitochondrial membrane potential Source: UniProtKB
- positive regulation of neuron death Source: Ensembl
- positive regulation of proteolysis Source: MGI
- positive regulation of release of cytochrome c from mitochondria Source: MGI
- positive regulation of T cell differentiation Source: MGI
- positive regulation of type B pancreatic cell development Source: UniProtKB
- regulation of apoptotic process Source: MGI
- regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- regulation of mitochondrial membrane permeability Source: UniProtKB
- release of cytochrome c from mitochondria Source: MGI
- response to amino acid Source: Ensembl
- response to calcium ion Source: Ensembl
- response to estradiol Source: Ensembl
- response to ethanol Source: Ensembl
- response to glucocorticoid Source: Ensembl
- response to glucose Source: Ensembl
- response to hydrogen peroxide Source: Ensembl
- response to oleic acid Source: Ensembl
- response to progesterone Source: Ensembl
- response to testosterone Source: Ensembl
- spermatogenesis Source: Ensembl
- suppression by virus of host apoptotic process Source: MGI
- type B pancreatic cell proliferation Source: UniProtKB
Keywordsi
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-MMU-111447. Activation of BAD and translocation to mitochondria. R-MMU-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members. R-MMU-193648. NRAGE signals death through JNK. |
Names & Taxonomyi
Protein namesi | Recommended name: Bcl2-associated agonist of cell deathShort name: BAD Alternative name(s): Bcl-2-binding component 6 Bcl-xL/Bcl-2-associated death promoter Bcl2 antagonist of cell death |
Gene namesi | Name:Bad Synonyms:Bbc6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096330. Bad. |
Subcellular locationi
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 112 | S → A: Enhances pro-apoptotic activity; no phosphorylation. 2 Publications | 1 | |
Mutagenesisi | 136 | S → A: No phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 155 | S → A: Enhances pro-apoptotic activity; no phosphorylation; interacts with Bcl-X(L). 1 Publication | 1 | |
Mutagenesisi | 155 | S → D: No pro-apoptotic activity, no interaction with Bcl-X(L). 1 Publication | 1 | |
Mutagenesisi | 170 | S → A: Enhances pro-apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 170 | S → D: No pro-apoptotic activity; even when associated with A-112. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL5385. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000143104 | 1 – 204 | Bcl2-associated agonist of cell deathAdd BLAST | 204 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 67 | PhosphoserineBy similarity | 1 | |
Modified residuei | 112 | Phosphoserine; by PKA, PKB, PIM2, PIM3, PAK1, PAK2, PAK4, PAK5, RPS6KA1 AND RAF16 Publications | 1 | |
Modified residuei | 128 | PhosphoserineBy similarity | 1 | |
Modified residuei | 131 | Asymmetric dimethylarginine; by PRMT1By similarity | 1 | |
Modified residuei | 133 | Asymmetric dimethylarginine; by PRMT1By similarity | 1 | |
Modified residuei | 134 | PhosphoserineBy similarity | 1 | |
Modified residuei | 136 | Phosphoserine; by PKA, PKB, PAK1, RPS6KA1, RPS6KB1 and PKC/PRKCQCombined sources5 Publications | 1 | |
Modified residuei | 155 | PhosphoserineCombined sources | 1 | |
Modified residuei | 155 | Phosphoserine; by PKA and PKBCombined sources1 Publication | 1 | |
Modified residuei | 170 | PhosphoserineCombined sources1 Publication | 1 |
Post-translational modificationi
Phosphorylated on one or more of Ser-112, Ser-136, Ser-155 and Ser-170 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-155, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-136 is the major site of AKT/PKB phosphorylation, Ser-155 the major site of protein kinase A (CAPK) phosphorylation.9 Publications
Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136.5 Publications
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q61337. |
MaxQBi | Q61337. |
PaxDbi | Q61337. |
PRIDEi | Q61337. |
PTM databases
iPTMneti | Q61337. |
PhosphoSitePlusi | Q61337. |
Miscellaneous databases
PMAP-CutDBi | Q61337. |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024959. |
CleanExi | MM_BAD. |
ExpressionAtlasi | Q61337. baseline and differential. |
Genevisiblei | Q61337. MM. |
Interactioni
Subunit structurei
Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-112/Ser-136 phosphorylated form binds 14-3-3 proteins. Interacts with AKT1 and PIM3 (By similarity). Interacts (via BH3 domain) with NOL3 (via CARD domain); preventing the association of BAD with BCL2 (By similarity). Interacts with HIF3A isoform 2 (via C-terminus domain); the interaction reduces the binding between BAD and BAX (PubMed:21546903).By similarity1 Publication
Binary interactionsi
GO - Molecular functioni
- 14-3-3 protein binding Source: Ensembl
- protein heterodimerization activity Source: MGI
- protein kinase B binding Source: Ensembl
- protein kinase binding Source: MGI
- protein phosphatase 2B binding Source: Ensembl
- protein phosphatase binding Source: MGI
Protein-protein interaction databases
BioGridi | 198296. 6 interactors. |
CORUMi | Q61337. |
DIPi | DIP-273N. |
ELMi | Q61337. |
IntActi | Q61337. 17 interactors. |
MINTi | Q61337. |
STRINGi | 10090.ENSMUSP00000025910. |
Chemistry databases
BindingDBi | Q61337. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 139 – 141 | Combined sources | 3 | |
Helixi | 142 – 160 | Combined sources | 19 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BZW | X-ray | 2.30 | B | 137-163 | [»] | |
DisProti | DP00563. | |||||
ProteinModelPortali | Q61337. | |||||
SMRi | Q61337. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q61337. |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 147 – 161 | BH3Add BLAST | 15 |
Domaini
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Sequence similaritiesi
Belongs to the Bcl-2 family.Curated
Phylogenomic databases
eggNOGi | ENOG410IXUQ. Eukaryota. ENOG410Y2J3. LUCA. |
GeneTreei | ENSGT00390000010740. |
HOGENOMi | HOG000095169. |
HOVERGENi | HBG001653. |
InParanoidi | Q61337. |
KOi | K02158. |
OMAi | IQSWWDR. |
OrthoDBi | EOG091G0Z5T. |
PhylomeDBi | Q61337. |
TreeFami | TF102001. |
Family and domain databases
InterProi | View protein in InterPro IPR018868. BAD. |
PANTHERi | PTHR28540. PTHR28540. 1 hit. |
Pfami | View protein in Pfam PF10514. Bcl-2_BAD. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q61337-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTPKQPSLA PAHALGLRKS DPGIRSLGSD AGGRRWRPAA QSMFQIPEFE
60 70 80 90 100
PSEQEDASAT DRGLGPSLTE DQPGPYLAPG LLGSNIHQQG RAATNSHHGG
110 120 130 140 150
AGAMETRSRH SSYPAGTEED EGMEEELSPF RGRSRSAPPN LWAAQRYGRE
160 170 180 190 200
LRRMSDEFEG SFKGLPRPKS AGTATQMRQS AGWTRIIQSW WDRNLGKGGS
TPSQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37296 mRNA. Translation: AAA64465.1. BC006762 mRNA. Translation: AAH06762.1. |
CCDSi | CCDS29513.1. |
PIRi | A55671. |
RefSeqi | NP_031548.1. NM_007522.3. |
UniGenei | Mm.4387. |
Genome annotation databases
Ensembli | ENSMUST00000025910; ENSMUSP00000025910; ENSMUSG00000024959. |
GeneIDi | 12015. |
KEGGi | mmu:12015. |
UCSCi | uc008gjn.2. mouse. |
Similar proteinsi
Entry informationi
Entry namei | BAD_MOUSE | |
Accessioni | Q61337Primary (citable) accession number: Q61337 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | March 28, 2018 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Caution
The protein name 'Bcl2 antagonist of cell death' may be misleading. The protein antagonises Bcl2-mediated repression of cell death, hence it promotes apoptosis.Curated